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Full-Text Articles in Life Sciences

Mutation Or Deletion Of The Saccharomyces Cerevisiae Rat3/Nup133 Gene Causes Temperature-Dependent Nuclear Accumulation Of Poly(A)+ Rna And Constitutive Clustering Of Nuclear Pore Complexes, Ou Li, Catherine V. Heath, David C. Amberg, Thomas C. Dockendorff, Connie S. Copeland, Michael Snyder, Charles N. Cole Oct 1995

Mutation Or Deletion Of The Saccharomyces Cerevisiae Rat3/Nup133 Gene Causes Temperature-Dependent Nuclear Accumulation Of Poly(A)+ Rna And Constitutive Clustering Of Nuclear Pore Complexes, Ou Li, Catherine V. Heath, David C. Amberg, Thomas C. Dockendorff, Connie S. Copeland, Michael Snyder, Charles N. Cole

Open Dartmouth: Faculty Open Access Scholarship

To identify genes whose products play potential roles in the nucleocytoplasmic export of messenger RNA, we isolated temperature-sensitive strains of Saccharomyces cerevisiae and examined them by fluorescent in situ hybridization. With the use of a digoxigen-tagged oligo-(dT)50 probe, we identified those that showed nuclear accumulation of poly(A)+ RNA when cells were shifted to the nonpermissive temperature. We describe here the properties of yeast strains bearing the rat3-1 mutation (RAT-ribonucleic acid trafficking) and the cloning of the RAT3 gene. When cultured at the permissive temperature of 23 degrees C, fewer than 10% of cells carrying the rat3-1 allele ...


Oral Administration Of Coprostanol Producing Microorganisms To Humans To Decrease Plasma Cholesterol Concentration, Donald C. Beitz, Jerry W. Young, Ling Li, Kimberly K. Buhman Aug 1995

Oral Administration Of Coprostanol Producing Microorganisms To Humans To Decrease Plasma Cholesterol Concentration, Donald C. Beitz, Jerry W. Young, Ling Li, Kimberly K. Buhman

Iowa State University Patents

A method of decreasing plasma cholesterol is disclosed. The method includes oral administration of coprostanol-producing bacteria to humans. These organisms, once in the small intestine, will reduce free cholesterol to coprostanol, which is absorbed poorly. Pharmaceutical compositions for delivery are included as are different methods of delivery such as placing the microorganisms in food.


Protein-Peptide Interactions Analyzed With The Yeast Two-Hybrid System, Meijia Yang, Zining Wu, Stanley Fields Apr 1995

Protein-Peptide Interactions Analyzed With The Yeast Two-Hybrid System, Meijia Yang, Zining Wu, Stanley Fields

Open Dartmouth: Faculty Open Access Scholarship

The yeast two-hybrid system was used to screen a library of random peptides fused to a transcriptional activation domain in order to identify peptides capable of binding to the retinoblastoma protein (Rb). Seven peptides were identified, allof which contain the Leu-X-Cys-X-Glu motif found in Rb-binding proteins, although their activity in the yeast assay variedover a 40-fold range. Mutagenesis of the DNA encoding two of these peptides followed by screening in the two-hybrid systemallowed the delineation of residues apart from the invariant Leu, Cys and Glu that affect binding to Rb. Binding affinities of a peptide and one of its variants ...


Assessing Sequential Oncogene Amplification In Human Breast Cancer, Laura E. Janocko, Joseph F. Lucke, David W. Groft, Kathryn A. Brown, Charles A. Smith, Agnese A. Pollice, Sarita G. Singh, Robert Yakulis, Robert J. Hartsock, Stanley E. Shackney Jan 1995

Assessing Sequential Oncogene Amplification In Human Breast Cancer, Laura E. Janocko, Joseph F. Lucke, David W. Groft, Kathryn A. Brown, Charles A. Smith, Agnese A. Pollice, Sarita G. Singh, Robert Yakulis, Robert J. Hartsock, Stanley E. Shackney

Joseph Lucke

Studies of amplification and/or overexpression of c-myc, HER-2/neu, and H-ras in breast cancer have shown that each is associated with a poor prognosis. The purpose of this study was to explore the possibility that there is a preferred sequence of amplification of these oncogenes in breast cancer. The frequencies of amplification and patterns of co-amplification of c-myc, HER-2/neu, and H-ras were studied in a group of 84 breast cancers. The data suggested a preferred sequence of amplification that consisted of c-myc amplification-HER-2/neu amplification-H-ras amplification. This model was supported by loglinear analysis. In addition, the levels of ...


A Phylogenetic Analysis Of Vertebrate And Invertebrate Notch-Related Genes, Eleanor M. Maine, James L. Lissemore, William T. Starmer Jan 1995

A Phylogenetic Analysis Of Vertebrate And Invertebrate Notch-Related Genes, Eleanor M. Maine, James L. Lissemore, William T. Starmer

Biology

Members of the Notch gene family are thought to mediate inductive cell-cell interactions during develop ment of a wide variety of vertebrates and invertebrates. These genes encode transmembrane proteins that appear to act as receptors and contain three repeated sequence motifs. Two of these motifs (an epidermal growth factor like sequence and a cdc10/SW16/ankyrin sequence) have been found in a large number of unrelated proteins, while the third motif (a lin-12/Notch/glp-1 sequence) is unique to proteins of the Notch family. We present a phylogenetic analysis of 17 Notch-related genes from eight species that has implications as ...


Molecular Analyses Of Group 1 Late Embryogenic Abundant (Lea) Genes In Soybean (Glycine Max (L.) Merr.) , Éberson Sanches Calvo Jan 1995

Molecular Analyses Of Group 1 Late Embryogenic Abundant (Lea) Genes In Soybean (Glycine Max (L.) Merr.) , Éberson Sanches Calvo

Retrospective Theses and Dissertations

The entire soybean Sle gene family was cloned and characterized. The five Sle genes (Sle1-5) could be divided into two classes based on sequence identity. Sle1-4 map to 4 different linkage groups. Sle1 and Sle5 are expressed weeks earlier in seed development than Sle2 and Sle3. Sle4 is a pseudogene. Maximal levels of Sle mRNA accumulate in the seeds, before desiccation had occurred, and decline rapidly upon seed imbibition. Desiccation does not induce Sle expression in seeds. Unlike most of its homologs, ABA is not necessary to induce Sle expression in cultured embryos and only moderately increases Sle mRNA levels ...


Cloning And Analysis Of The Autonomous Element, Mudr:Cy, Of The Mutator Family Of Maize , An-Ping Hsia Jan 1995

Cloning And Analysis Of The Autonomous Element, Mudr:Cy, Of The Mutator Family Of Maize , An-Ping Hsia

Retrospective Theses and Dissertations

An autonomous Cy transposable element of Zea mays inserted at the a1 locus (a1-m5216: MuDR) was isolated genetically and molecularly cloned. Restriction mapping and sequencing results indicate that Cy (designated MuDR:Cy1) is another isolate of the autonomous element of the Mutator family, MuDR. Six defective derivative alleles of a1-m5216:MuDR were isolated genetically and molecularly cloned. Five of these a1-r alleles (a1-r5835, a1-r5431, a1-r182, a1-r5938 and a1-r5306) behave like non-autonomous elements in that their transposition can only be activated by active MuDR elements in trans. Three of these a1-r alleles have deletions that disrupt transcript mudrA. One allele has ...


Carbon Allocation And Partitioning In Aspen Clones Varying In Sensitivity To Tropospheric Ozone, M.D. Coleman, R.E. Dickson, J.G. Isebrands, D.F. Karnosky Jan 1995

Carbon Allocation And Partitioning In Aspen Clones Varying In Sensitivity To Tropospheric Ozone, M.D. Coleman, R.E. Dickson, J.G. Isebrands, D.F. Karnosky

Aspen Bibliography

No abstract provided.


The Role Of D-Raf In The Terminal Class Signal Transduction Pathway In Drosophila Melanogaster , Kwang-Hyun Baek Jan 1995

The Role Of D-Raf In The Terminal Class Signal Transduction Pathway In Drosophila Melanogaster , Kwang-Hyun Baek

Retrospective Theses and Dissertations

This dissertation describes the characterization of D-raf-mediated signal transduction in the establishment and elaboration of pattern in Drosophila melanogaster. D- raf is the Drosophila homologue of the human Raf-1 proto-oncogene. As a member of the Drosophila Torso (Tor) signal transduction cascade, D-raf acts to define cellular fates at the embryonic poles. Here we show that the serine/threonine kinase activity of D-raf is required for propagation of the Tor signal and that human Raf-1 can substitute for D-raf in this pathway. We identified two serine phosphorylation sites (S388 and S743) and found that serine or its phosphorylation at site 743 ...


Analysis And Application Of The En Transposable Element And Genetic Study Of Resistance To Bipolaris Maydis In Maize , Ru-Ying Chang Jan 1995

Analysis And Application Of The En Transposable Element And Genetic Study Of Resistance To Bipolaris Maydis In Maize , Ru-Ying Chang

Retrospective Theses and Dissertations

Since its original isolation and molecular characterization, the En (Enhancer) transposable element has been studied extensively. It also has been used as a tool to tag and isolate genes and to elucidate mechanisms of transposition and gene regulation. The first aspect of this dissertation is on the analysis and application of En. Three En-mutable alleles at the maize A2 locus harboring an autonomous En element, but showing different somatic variegation patterns, were studied by PCR and sequence analysis. It is shown that a fine variegation type (late reversions) is caused by En insertion in the 5[superscript]' region, whereas two ...


G95-1271 Mastitis Is A Disease -- Control Is An Everyday Task, Gerald R. Bodman, Duane N. Rice Jan 1995

G95-1271 Mastitis Is A Disease -- Control Is An Everyday Task, Gerald R. Bodman, Duane N. Rice

Historical Materials from University of Nebraska-Lincoln Extension

A comprehensive mastitis control program will effectively control infections caused by both environmental and contagious pathogens.

Herd mastitis problems can be caused by both environmental and contagious pathogens (disease-causing organisms). These problems may occur separately or simultaneously. Various testing techniques are necessary to determine the type of causative organism and the infection level of the herd or individual cow. Appropriate tests include the CMT (California Mastitis Test), milk market somatic cell count (SCC), Dairy Herd Improvement Association (DHIA) SCC and laboratory cultures of milk from infected cows. Depending upon test results, mastitis control strategies may need to be changed to ...


Genetics Of The Stable Fly., David B. Taylor Dec 1994

Genetics Of The Stable Fly., David B. Taylor

David B. Taylor

No abstract provided.