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Full-Text Articles in Life Sciences

Light-Induced Expression Of A Blue Coral Protein In An Industrial Fungus, Lindsay Tomczak Jun 2018

Light-Induced Expression Of A Blue Coral Protein In An Industrial Fungus, Lindsay Tomczak

Honors Theses

The ultimate goal of this research project is to improve the growth and structural characteristics of an Ecovative Design LLC (Green Island, NY) production strain to produce commercial biomaterials for packaging. These biomaterials are produced from renewable resources and can be easily broken down after they fulfill their purpose, unlike the commonly used materials today (such as Styrofoam). In an effort to quantify the light-reactivity of the fungus, a codon-optimized DNA sequence coding a blue chromoprotein was introduced and utilized as a visual reporter gene. Transcriptional controlling sequences were identified from orthologs to specific light-regulated genes and were combined with ...


Population Genetics Of Bowfins (Amiidae, Amia Spp.) Across The Laurentian Great Lakes And The Carolinas, Madeline J. Clark Dec 2015

Population Genetics Of Bowfins (Amiidae, Amia Spp.) Across The Laurentian Great Lakes And The Carolinas, Madeline J. Clark

Honors Theses

The Bowfin, Amia calva Linneaus (1766), is a common Eastern North American fish and the last extant member of the order Amiiformes. By 1870, twelve additional species of Bowfin had been described from widely dispersed localities from lakes Huron and Champlain in the north to Charleston, SC, and New Orleans, LA, in the south. This diversity of nominal forms was synonymized into a single species, A. calva, by Jordan and Evermann in 1896. Since then, this monotypy hypothesis has been generally accepted, but never scientifically validated. Recently, this hypothesis was challenged when morphological analyses of Bowfin from Savanah River, SC ...


Characterization Of Sox Family Members In Sponge Stem Cells And During Development, Karen E. Leeds Apr 2008

Characterization Of Sox Family Members In Sponge Stem Cells And During Development, Karen E. Leeds

Honors Theses

Sponges are considered the oldest and most basal part of the metazoan lineage and therefore possess a unique set of gene families that are highly conserved among all animals. One of these gene families is known as Sox transcription factors. Sox genes are known to play important roles in complex animals such as the specification of the primary layers of the body, determination of sex, and most recently induction of induced pluirpotent stem cells (iPS cells) from both human and mouse fibroblasts with the help of three other transcription factors. We have found that two different demosponge species Halichondria bowerbanki ...


Characterization Of A Non-Ribosomal Peptide From Agrobacterium Tumefaciens, Graham Strub May 2001

Characterization Of A Non-Ribosomal Peptide From Agrobacterium Tumefaciens, Graham Strub

Honors Theses

The current efforts underway to sequence the genome of Agrobacterium tumefaciens have led to the discovery of a large region of DNA (52kb) with a strong homology to several polyketide synthetase genes. This was a matter of great interest to the Agrobacterium project; no current literature documents the production of a polyketide or non:iibosomal peptide from Agrobacterium. Furthermore, the multi-domain enzymes coded by these polyketide synthetase genes in other bacterial species are responsible for synthesizing several toxins, many which act as antibiotic agents {8, 9, 12, 16). This was also of great interest, since Agrobacterium is not known to ...


Scientific Investigation Of The Plant Pathogen Agrobacterium Tumefaciens, Casey M. Flanagan, Christopher B. Crowell Ii, Bryan P. Markelz Jan 2000

Scientific Investigation Of The Plant Pathogen Agrobacterium Tumefaciens, Casey M. Flanagan, Christopher B. Crowell Ii, Bryan P. Markelz

Honors Theses

A. tumefaciensis a plant pathogen and causative agent of Crown Gall Disease, an ailment that results in the development of tumors in plants. It is a unique organism in that it is the only known non-viral pathogen that transfers its DNA into the host cell. Consequently, the majority of the research conducted on A. tumefaciensinvestigates its mechanism of infection. However, A. tumefaciensis also rare to the bacterial world in that its genome is comprised of two chromosomes: one circular and one linear. Most other bacteria only have one circular chromosome. This area of research has been grossly overlooked and therefore ...