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Wish4campus: Evaluating College Food Insecurity And Promoting Solutions For Student Wellbeing, Rebecca L. Hagedorn Jan 2019

Wish4campus: Evaluating College Food Insecurity And Promoting Solutions For Student Wellbeing, Rebecca L. Hagedorn

Graduate Theses, Dissertations, and Problem Reports

Introduction: Interest in college food insecurity has increased in previous years, however, little research focuses on the Appalachian and Southeastern regions of the United States resulting in limited understanding of food insecurity’s impact on college students in these regions. Additionally, resources to help food insecure students are often sparse with universities lacking evidence-based programming to implement for student benefit.

Aims: This dissertation aims to (1) investigate the correlates and behavioral consequences of food insecurity on college students at an Appalachian university, (2) expand college food insecurity research to a regional investigation in the Appalachian and Southeastern regions, and (3 ...


Apple Pomace As A Novel Aid For Western Diet-Induced Nonalcoholic Fatty Liver Disease In Young Female Sprague Dawley Rats, R. Chris Skinner Jan 2019

Apple Pomace As A Novel Aid For Western Diet-Induced Nonalcoholic Fatty Liver Disease In Young Female Sprague Dawley Rats, R. Chris Skinner

Graduate Theses, Dissertations, and Problem Reports

Apple pomace is a “waste” byproduct of apple processing that causes environmental pollution and is costly to dispose of. Yet, apple pomace is rich in dietary fibers and antioxidants. Analysis of apple pomace’s nutritional profile indicates suitability as a potential dietary treatment for non-alcoholic fatty liver disease (NAFLD) and the more severe non-alcoholic steatohepatitis (NASH). NAFLD is the most prevalent liver disease in the world with prevalence and severity expected to increase in both adults and children. Currently, there is no approved drug treatment for NAFLD and therefore, dietary intervention is the primary treatment. The study objectives were to ...


Demographic, Spatial, And Epigenetic Response Of The Louisiana Waterthrush (Parkesia Motacilla) To Shale Gas Development, Mack W. Frantz Jan 2019

Demographic, Spatial, And Epigenetic Response Of The Louisiana Waterthrush (Parkesia Motacilla) To Shale Gas Development, Mack W. Frantz

Graduate Theses, Dissertations, and Problem Reports

My study centered on a bioindicator songbird, the Louisiana Waterthrush (Parkesia motacilla), hereafter waterthrush, an organism that co-occurs in both forested and aquatic habitat across the aquatic-terrestrial interface. This enabled the opportunity to quantify demographic, spatial, and epigenetic (i.e., DNA methylation) responses in a highly forested watershed of the Central Appalachians, the areas that have undergone the most rapid transformations over the last decade from unconventional shale gas development and activities. I organized my dissertation into 4 parts (Part 1: Introduction, Part 2: Louisiana Waterthrush Demography, Part 3: Spatial Assessment of Louisiana Waterthrush Foraging, Part 4: Louisiana Waterthrush Molecular ...


Soil Microbial Succession Following Surface Mining Is Governed Primarily By Deterministic Factors, Jennifer Lynne Kane Jan 2019

Soil Microbial Succession Following Surface Mining Is Governed Primarily By Deterministic Factors, Jennifer Lynne Kane

Graduate Theses, Dissertations, and Problem Reports

Understanding the successional dynamics governing soil microbial community assembly is a promising way to advance development of remediation strategies for lands disturbed by anthropogenic activities. The environmental and ecological influences shaping these communities following soil disturbance remain only partially understood. One example of a physical anthropogenic disturbance is extraction of minerals such as coal by surface mining. Surface mining removes natural soils and these soils may be replaced immediately on adjacent reclaimed areas or they may be stored in piles for later use. During reclamation, the soil is replaced on the landscape and the site is re-vegetated with grasses and ...


Regulation Of The Long Non-Coding Rna Fam83h-As1 By Human Papillomavirus In Cervical Cancer, Jamie Ann Barr Ph.D. Jan 2019

Regulation Of The Long Non-Coding Rna Fam83h-As1 By Human Papillomavirus In Cervical Cancer, Jamie Ann Barr Ph.D.

Graduate Theses, Dissertations, and Problem Reports

Non-coding RNAs (NcRNAs), such as long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), have been found to be involved in a variety of critical biological processes, and dysregulation of ncRNAs have been involved with several human diseases including cancer.

High-risk human papillomavirus (HPV) infection is one of the first events in the process of carcinogenesis in cervical and a subset of head and neck cancers. The expression of the viral oncoproteins E6 and E7 is essential in this process by inactivating the tumor suppressor proteins p53 and Rb, respectively, in addition to their interactions with other host proteins and regulation of ...


Aptamer Functionalized Zinc Oxide Field Effect Transistors For Odor Detection, Michael D. Aldridge Jan 2019

Aptamer Functionalized Zinc Oxide Field Effect Transistors For Odor Detection, Michael D. Aldridge

Graduate Theses, Dissertations, and Problem Reports

Odor detection and identification are complex processes, and tasks that currently only animals do well. There is a pressing need for an electronic nose, or eNose, with good sensitivity, selectivity, and speed that mimics that ability. Food quality control operations, environmental sensing, occupational safety, and the defense sectors all require systems that can rapidly and reliably detect trace levels of volatile organic compounds. The goal of this work is to create a biologically inspired device which can accurately detect and identify odors at concentrations consistent with the most sensitive biological systems.

In order to mimic a natural olfactory system, we ...


Utilizing The Parts Of Common Food Products As Functional Ingredients Part 1: Separation And Concentration Of Ω-3 Pufa-Rich Phospholipids By Hydration Of Krill Oil Part 2: Functional Properties Of A Concentrated Egg Yolk Protein Powder Using A One-Step Organic Solvent Extraction Process, Casey Lynn Showman Jan 2019

Utilizing The Parts Of Common Food Products As Functional Ingredients Part 1: Separation And Concentration Of Ω-3 Pufa-Rich Phospholipids By Hydration Of Krill Oil Part 2: Functional Properties Of A Concentrated Egg Yolk Protein Powder Using A One-Step Organic Solvent Extraction Process, Casey Lynn Showman

Graduate Theses, Dissertations, and Problem Reports

The food industry separates food products into different parts to better utilize the functional and nutritional benefits of each fraction. The overall focus of this thesis is on separating and utilizing fractions and co-products from food manufacturing as functional ingredients. Therefore, the objective of this research was to 1) evaluate the effectiveness of water degumming to separate omega-3 PUFA-rich phospholipids from other lipids found in krill oil and 2) determine how different organic solvents would affect physiochemical and functional properties of egg yolk protein recovered from spray-dried egg yolk using a novel one-step organic solvent extraction method.

The aim of ...


Characterization Of Lipids And The Protein Co-Products From Various Food Sources Using A One-Step Organic Solvent Extraction Process, Alleda Rose Jan 2019

Characterization Of Lipids And The Protein Co-Products From Various Food Sources Using A One-Step Organic Solvent Extraction Process, Alleda Rose

Graduate Theses, Dissertations, and Problem Reports

Traditional lipid extraction methods are labor intensive and time-consuming so interest in one-step methods using various organic solvents are gaining in popularity. Extraction efficiency depends heavily on the solvents used yet comparisons between different solvents are lacking. Defatting techniques are commonly used to concentrate crude protein and may be useful for enhancing protein content in food sources. The objective of this research was to 1) determine the lipid extraction efficiencies of hexane (H), 3:2 hexane-isopropanol (HI), chloroform (C), 2:1 chloroform-methanol (CM), and methyl-tert-butyl-ether (MTBE) using a one-step organic solvent lipid extraction method on whole insect, egg yolk, and ...


Analysis Of User Preference With N95 And Powered Air-Purifying Respirators In A Healthcare Work Environment, Brittany Renee Carver Jan 2019

Analysis Of User Preference With N95 And Powered Air-Purifying Respirators In A Healthcare Work Environment, Brittany Renee Carver

Graduate Theses, Dissertations, and Problem Reports

For those working in the healthcare field, personal protective equipment is vital. Healthcare workers are often first responders when it comes to dealing with patients who are infected or when a disease outbreak occurs. The Occupational Safety and Health Administration (OSHA) requires all healthcare operations to provide employee protection against blood borne pathogens. With the increase of life-threatening disease outbreaks, such as SARS in 2003 and Ebola in 2014, it is of the upmost importance to make sure respirators are best suited for users and acceptable for long term use. It has been shown through previous studies that due to ...


Probability Discounting Of Lewis And Fischer 344 Rats: Strain Comparisons At Baseline And Following Acute Administration Of D-Amphetamine, Jenny E. Ozga Jan 2019

Probability Discounting Of Lewis And Fischer 344 Rats: Strain Comparisons At Baseline And Following Acute Administration Of D-Amphetamine, Jenny E. Ozga

Graduate Theses, Dissertations, and Problem Reports

Risky choice can be defined as choice for a larger, uncertain reinforcer over a smaller, certain reinforcer when choosing the smaller alternative maximizes reinforcement. Risky choice is studied using various procedures in the animal laboratory; one such procedure is called probability discounting. There are many variables that contribute to risky decision-making, including biological and pharmacological determinants. The present study assessed both of these variables by evaluating dose-response effects of d-amphetamine on risky choice of Lewis (LEW) and Fischer 344 (F344) rats. The probability-discounting procedure included discrete-trials choices between one food pellet delivered 100% of the time and three food ...


Analysis Of Unconventional Oil And Gas Impacts On Downstream Fish Assemblages And Physiological Stress, Joshua Noah Ankeny Jan 2019

Analysis Of Unconventional Oil And Gas Impacts On Downstream Fish Assemblages And Physiological Stress, Joshua Noah Ankeny

Graduate Theses, Dissertations, and Problem Reports

Unconventional Oil and Gas (UOG) production has been steadily expanding throughout the mid-Atlantic since 2008. Increased sedimentation, degraded water chemistry and an overall decrease in habitat quality due to UOG is anticipated to negatively impact aquatic inhabitants, a common observation in other stressed landscapes (i.e., mining, agriculture, development). We assessed stream health through both community analysis and physiological parameters (i.e., growth and hematocrit). A before-after-control-impact study uncovered three fish metrics that were significantly different following UOG disturbances. The invertivore-piscivore metric decreased following UOG disturbance (p = 0.045) whereas two benthic metrics saw a surprising increase (p = 0.003 ...


Ecological Benefits Of Watershed-Scale Restoration In Two Intensively Mined Cold- And Warm-Water Ecosystems, Rebecca Anne Long Jan 2019

Ecological Benefits Of Watershed-Scale Restoration In Two Intensively Mined Cold- And Warm-Water Ecosystems, Rebecca Anne Long

Graduate Theses, Dissertations, and Problem Reports

Stream restoration projects are increasingly common. However, restoration projects that establish measurable goals, have pre- and post-restoration monitoring, and are implemented at the watershed scale are rare. We conducted a long-term (9-year) before-after-control-impact designed assessment of two watershed-scale acid mine drainage (AMD) remediation programs, one in a warm-water ecosystem and one in a cold-water ecosystem in West Virginia, USA. Restoration was strategically designed to recover biodiversity and improve the native fisheries by restoring chemically degraded water quality and re-establishing riverscape connectivity. We used analysis of variance to quantify responses in water chemistry, benthic macroinvertebrate communities, and fish community composition before ...


The Role Of Sirt1 In Skeletal Muscle Function And Repair Of Older Mice, Matthew John Myers Jan 2019

The Role Of Sirt1 In Skeletal Muscle Function And Repair Of Older Mice, Matthew John Myers

Graduate Theses, Dissertations, and Problem Reports

Human skeletal muscle is a highly metabolic tissue necessary for mobility and coordination. Responsible for approximately one-fifth of the resting human metabolism, skeletal muscle is also an important regulator of metabolites like glucose and contributes to the regulation of body temperature. Although there is a gradual decline in muscle mass associated with aging, a certain percentage of the population suffer from severe muscle mass and strength deterioration, classified as sarcopenia (5-13% for people aged 60-70 years old, 11-50% for those 80 or older). Sarcopenia is linked to increased morbidity and mortality rates in the elderly population, while annual healthcare costs ...


Mine Reclamation Using Biofuel Crops: Insights Into The Microbial Ecology Of The Switchgrass (Panicum Virgatum) Microbiome, Brianna L. Mayfield Jan 2019

Mine Reclamation Using Biofuel Crops: Insights Into The Microbial Ecology Of The Switchgrass (Panicum Virgatum) Microbiome, Brianna L. Mayfield

Graduate Theses, Dissertations, and Problem Reports

Bioenergy crop production has steadily increased due to growing political support for renewable energy, thus initiating a demand to find alternative agricultural land. An innovative option is the use of marginal soils, such as reclaimed mine lands, to produce bioenergy crops. Switchgrass (Panicum virgatum) is a promising bioenergy crop that can be grown on marginal lands due to its robust growth in various soil types and climates. However, little is known regarding plant-microbe interactions among switchgrass systems within reclaimed mine lands. A study conducted in 2008 grew switchgrass on high- and low- quality reclaimed mine sites (Hampshire and Hobet, respectively ...


Genet-Cnv: Boolean Implication Networks For Modeling Genome-Wide Co-Occurrence Of Dna Copy Number Variations, Salvi Singh Jan 2019

Genet-Cnv: Boolean Implication Networks For Modeling Genome-Wide Co-Occurrence Of Dna Copy Number Variations, Salvi Singh

Graduate Theses, Dissertations, and Problem Reports

Lung cancer is the leading cause of cancer-related death in the world. Lung cancer can be categorized as non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). NSCLC makes up about 80% to 85% of lung cancer cases diagnosed, whereas SCLC is responsible for 10% to 15% of the cases. It remains a challenge for physicians to identify patients who shall benefit from chemotherapy. In such a scenario, identifying genes that can facilitate therapeutic target discoveries and better understanding disease mechanisms and their regulation in different stages of lung cancer, remains an important topic of research.

In this ...


Sodium Channel Distribution In The Apical Dendrites Of Pyramidal Cells Vary In The Hindbrain Of Apteronotus Leptorhynchus., Sree Indrani Motipally Jan 2019

Sodium Channel Distribution In The Apical Dendrites Of Pyramidal Cells Vary In The Hindbrain Of Apteronotus Leptorhynchus., Sree Indrani Motipally

Graduate Theses, Dissertations, and Problem Reports

Apteronotid weakly electric fish heavily rely on their electrosensory system for behaviors like spatial navigation, communication and prey capture. Since the behaviorally important information about their environment is contained in the spatial and temporal modulations of the electrosensory signal, efficient mechanisms to process this information with great fidelity are of the utmost importance. Efficient sensory processing often involves having multiple parallel processing streams so that each stream can specialize to treat signals with different properties. This strategy requires the response properties and neural dynamic to be adjusted in each pathway to implement different neural coding strategies. One of the neural ...


Avian Occupancy Dynamics And Canada Warbler (Cardellina Canadensis) Environmental Correlates On A Central Appalachian Elevation Gradient In West Virginia, Gordon W. Dimmig Jan 2019

Avian Occupancy Dynamics And Canada Warbler (Cardellina Canadensis) Environmental Correlates On A Central Appalachian Elevation Gradient In West Virginia, Gordon W. Dimmig

Graduate Theses, Dissertations, and Problem Reports

Mountains are important areas for avian diversity and conservation since they have steep elevational and environmental gradients. In the central Appalachian Mountains, several songbird species have trailing edge populations that are restricted to high elevations. Climate change and other factors can cause species distributions to change, which is of particular concern for birds in restricted trailing edge populations. Additionally, the Canada Warbler (Cardellina canadensis) breeds throughout the Appalachian Mountains and is species of conservation concern due to range-wide population declines. For this project, I used avian point count data from the central Appalachian Mountains to: (1) evaluate the spatial and ...


Evaluating The Effects Of Exogenous Enzyme Supplementation On Broiler Growth, Victoria E. Ayres Jan 2019

Evaluating The Effects Of Exogenous Enzyme Supplementation On Broiler Growth, Victoria E. Ayres

Graduate Theses, Dissertations, and Problem Reports

Experiments were conducted to investigate the effects of exogenous enzymes in poultry diets. In Chapter 4, a study was conducted to determine the effects of a corn-expressed recombinant carbohydrase (AC1) on broiler performance and digesta viscosity in high non-starch polysaccharide (NSP) diets through 21 days of age. One day-old Hubbard × Cobb 500 chicks were assigned to 6 dietary treatments. Each treatment consisted of 12 replicate pens of 10 birds. The positive control diet (PC) was a corn and soybean meal formulation. The negative control diet (NC) included 10% wheat and 10% corn distiller’s dried grains with solubles (DDGS). The ...


The Effect Of Feed Manufacture Techniques On Pathogen Reduction And Feed Nutrient Value, Timothy Paul Boltz Jan 2019

The Effect Of Feed Manufacture Techniques On Pathogen Reduction And Feed Nutrient Value, Timothy Paul Boltz

Graduate Theses, Dissertations, and Problem Reports

Experiments were conducted to evaluate how differing feed manufacture techniques, such as varying conditioning times and new equipment, can affect pathogen mitigation, feed manufacture metrics, amino acid digestibility, and enzyme recovery in feed. Feed hygienics are of increasing importance in providing safe feed to animals, and ultimately safe food for consumers. Salmonella has been identified as a major microbial hazard in animal feed that has been linked to animal and human illness. In chapter 2, the effects of standard pelleting and more thermally aggressive techniques were utilized to determine pathogen mitigation potential. Use of antibiotics has decreased in recent years ...


Understanding Global Citizenship: Extension Employees’ Perceptions, Attitudes, And Opinions, Denis M. Scott Jan 2019

Understanding Global Citizenship: Extension Employees’ Perceptions, Attitudes, And Opinions, Denis M. Scott

Graduate Theses, Dissertations, and Problem Reports

Extension services at land-grant universities typically deliver different aspects of global citizenship education for youth through a variety of activities and instructional methods. An examination of the literature reveals that although gaps in content areas and barriers to programming do exist, there are many types of experiences available in multiple settings for youth to learn about global citizenship. These activities vary in terms of content topics, duration, and delivery methods. The purpose of the study is to determine Extension employees’ perceptions about global citizenship education, their attitudes about what content students should learn, their opinions about how they learn about ...


The Economic Contribution Of West Virginia's Forest Products Industry, Charles Clinton Gabbert Jan 2019

The Economic Contribution Of West Virginia's Forest Products Industry, Charles Clinton Gabbert

Graduate Theses, Dissertations, and Problem Reports

The hardwood forests that cover West Virginia have historically been an important resource to the state. The turbulent economic conditions resulting from the U.S. housing market collapse of the mid-2000s and subsequent economic recession have cause immense disruptions in the country’s forest products industry (FPI). An assessment of the economic contribution of the state’s FPI and the 7 major forest product sectors which comprise it was completed using the input-output economic modeling software IMPLAN with 2017 data. The historical contribution of the state industry was then assessed and compared for the years 2006, 2010, 2015, and 2017 ...


Characterization Of Ovine Monocytes In Response To Haemonchus Contortus Larvae In Vitro And A Novel Role Of Interleukin-13 Inducing Larval Paralysis, Elizabeth Shepherd Jan 2019

Characterization Of Ovine Monocytes In Response To Haemonchus Contortus Larvae In Vitro And A Novel Role Of Interleukin-13 Inducing Larval Paralysis, Elizabeth Shepherd

Graduate Theses, Dissertations, and Problem Reports

Haemonchus contortus is a hematophagous, gastrointestinal parasite affecting small ruminants. Parasitism by H. contortus is the greatest concern of sheep producers in the United States and accounts for billions of dollars in economic loss worldwide. However, different breeds of sheep display differences n natural resistance to H. contortus. St. Croix hair sheep have a remarkable ability to clear H. contortus without the aid of anthelmintics and develop a robust T helper type 2 (Th2) immune response, preventing establishment of infective stage 3 larvae (L3), when compared to susceptible Suffolk sheep. However, the mechanism of development of immune response and differences ...


Determining The Role Of Nudt7 In The Regulation Of Cellular Coa Levels And Metabolism, Stephanie Anne Shumar Jan 2019

Determining The Role Of Nudt7 In The Regulation Of Cellular Coa Levels And Metabolism, Stephanie Anne Shumar

Graduate Theses, Dissertations, and Problem Reports

Coenzyme A (CoA) is an essential cofactor required for hundreds of metabolic processes. Because it is such a critical cofactor, CoA levels are tightly regulated. In the fasted state and in diabetic mice, the concentration of CoA increases dramatically in the liver. This phenotype is associated with constitutively low CoA degradation, a process that is emerging as a potentially important mechanism for CoA regulation. Nudt7 and Nudt19 are two mammalian peroxisomal enzymes with CoA-degrading activity, which are highly expressed in the liver and kidney, respectively. Limited information is available on the biochemistry of Nudt7 and Nudt19; the structural basis for ...


Amazon Nights Ii: Electric Boogaloo-Neural Adaptations For Communication In Three Species Of Weakly Electric Fish, Kathryne M. Allen Jan 2019

Amazon Nights Ii: Electric Boogaloo-Neural Adaptations For Communication In Three Species Of Weakly Electric Fish, Kathryne M. Allen

Graduate Theses, Dissertations, and Problem Reports

Sensory systems have to extract useful information from environments awash in noise and confounding input. Studying how salient signals are encoded and filtered from these natural backgrounds is a key problem in neuroscience. Communication is a particularly tractable tool for studying this problem, as it is a ubiquitous task that all organisms must accomplish, easily compared across species, and is of significant ethological relevance. In this chapter I describe the current knowledge of what is both known and still unknown about how sensory systems are adapted for the challenges of encoding conspecific signals, particularly in environments complicated by conspecific-generated noise ...


Morphological And Functional Evidence Of Sexual Dimorphism In The Retrochiasmatic Area Population Of Nk3r-Containing Neurons In Sheep, Justin A. Lopez Jan 2019

Morphological And Functional Evidence Of Sexual Dimorphism In The Retrochiasmatic Area Population Of Nk3r-Containing Neurons In Sheep, Justin A. Lopez

Graduate Theses, Dissertations, and Problem Reports

Neurokinin B (NKB) and kisspeptin are critical for fertility and potent stimulators of gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) secretion in sheep. We previously demonstrated that administration of NKB receptor (NK3R) agonist, senktide, within the retrochiasmatic area (RCh) resulted in surge-like release of LH and increased expression of kisspeptin neurons in the arcuate nucleus (ARC) of female lambs. We concluded that NKB-responsive neurons in the RCh stimulate GnRH secretion by inducing kisspeptin release from neurons in the ARC.

We have demonstrated morphological and functional evidence of sexual dimorphism in NKB-NK3R signaling in ovine RCh. RCh senktide administration resulted in ...


Estimating The Age Of A Bloodstain Using Droplet Digital Pcr, Kalee Rae Crampton Jan 2019

Estimating The Age Of A Bloodstain Using Droplet Digital Pcr, Kalee Rae Crampton

Graduate Theses, Dissertations, and Problem Reports

Biological evidence is extremely valuable in the investigation of a crime due to the presence of DNA. DNA evidence is considered the gold standard in court cases due to its ability to link a suspect to a piece of evidence. In addition to DNA evidence, biological stains have the potential to provide a temporal link between an individual and a crime scene. Previous studies have shown that relative rates of RNA degradation can be used in order to estimate the age of bloodstains. Here, we examined the ability of droplet digital PCR to be used in place of quantitative PCR ...


Service Dogs For Veterans With Ptsd: Taxonomy, Work Stress Reduction, And Matching, Lindsay Parenti Jan 2019

Service Dogs For Veterans With Ptsd: Taxonomy, Work Stress Reduction, And Matching, Lindsay Parenti

Graduate Theses, Dissertations, and Problem Reports

Research suggests that many veterans with post-traumatic stress disorder (PTSD) struggle with reintegration (Sayer et al., 2010), but are unlikely to seek help or complete treatment (Schottenbauer et al., 2008). To make matters worse, available treatment options are often time consuming, challenging, and/or associated with negative side effects (Carafano & Hutchinson, 2017). Using animals as a treatment modality for veterans with PTSD is an emerging topic of interest and has shown promise (Owen, et al., 2016; Richie et al., 2016). However, several factors have hindered the advancement of this field. Obstacles include a lack of standard terminology and classification system, a need for empirical research to support the effectiveness of animal assisted interventions, and consideration of individual differences in response to animal assisted interventions. This investigation ...


Characterization Of Nodule-Specific, Slac And Mate Membrane Transporters In Medicago Truncatula, Lina Yang Jan 2019

Characterization Of Nodule-Specific, Slac And Mate Membrane Transporters In Medicago Truncatula, Lina Yang

Graduate Theses, Dissertations, and Problem Reports

Legumes play a crucial role in sustainable agriculture because of their intrinsic ability to reduce atmospheric N2 into NH3 via symbiotic nitrogen fixation (SNF). SNF is carried out in the symbiosome, a quasi-organelle containing endosymbiotic rhizobial bacteria in the cytoplasm of infected cells of the nodule. Therefore, the bacteria are surrounded by a symbiosome membrane (SM), which is derived from the plant plasma membrane during infection. SNF requires constant nutritional exchanges between symbionts, including reduced carbon (dicarboxylates) from the plant for reduced nitrogen (NH4+) from the bacteroids. This exchange of nutrients and signals is fundamental to SNF ...


Learn First, Practice Second Approach To Increase Health Professionals’ Nutrition-Related Knowledge, Attitudes, And Self-Efficacy., Madison E. Santella Jan 2019

Learn First, Practice Second Approach To Increase Health Professionals’ Nutrition-Related Knowledge, Attitudes, And Self-Efficacy., Madison E. Santella

Graduate Theses, Dissertations, and Problem Reports

The aim of this study was to assess the effectiveness of a “learn first, practice second” intervention on the nutrition-related knowledge, attitudes, and self-efficacy of multidisciplinary health professionals in West Virginia, specifically highlighting the Mediterranean Diet. Data was captured via online surveys at four time points (Baseline, Post-education, Post-immersion, Follow Up). All information was self-reported. This approach consisted of 16 weeks of online education, 2 weeks of cultural immersion in Tuscany, Italy, and a 7 month return to practice period. Data were analyzed using JMP Version Pro 12.2 and SAS Version 9.3 for Windows. Repeated measures ANOVA with ...