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Full-Text Articles in Life Sciences

Detection Of Heteroplasmic Single Nucleotide Polymorphisms Using Melt Curve Analysis And Dual Labeled Fluorescent Probes, Emily Jezewski Apr 2019

Detection Of Heteroplasmic Single Nucleotide Polymorphisms Using Melt Curve Analysis And Dual Labeled Fluorescent Probes, Emily Jezewski

Honors Theses, University of Nebraska-Lincoln

Plant mitochondrial genomes are strange – they are unusually large, consist of huge amounts of non-coding DNA, and contain of several overlapping regions throughout the genome. The genome is made of several different sized linear and circular molecules and different mitochondria within a cell will have different pieces of the genome. Even if multiple mitochondria contain the same region of the genome, these sequences can differ by single nucleotide polymorphisms (SNPS). This is known as heteroplasmy. Heteroplasmy has been documented in Arabidopsis thaliana using whole-genome sequencing data. While heteroplasmy is well-documented, its existence in the mitochondrial genome is unexpected and it ...


Plant Mitochondrial Genome Evolution And Structure Has Been Shaped By Double-Strand Break Repair And Recombination, Emily Wynn Apr 2019

Plant Mitochondrial Genome Evolution And Structure Has Been Shaped By Double-Strand Break Repair And Recombination, Emily Wynn

Dissertations and Theses in Biological Sciences

Plant mitochondrial genomes are large but contain a small number of genes. These genes have very low mutation rates, but genomes rearrange and expand at significant rates. We propose that much of the apparent complexity of plant mitochondrial genomes can be explained by the interactions of double-strand break repair, recombination, and selection. One possible explanation for the disparity between the low mutation rates of genes and the high divergence of non-genes is that synonymous mutations in genes are not truly neutral. In some species, rps14 has been duplicated in the nucleus, allowing the mitochondrial copy to become a pseudogene. By ...


Genome-Wide Characterization, Molecular Evolution And Mexpression Profiling Of The Metacaspases In Potato (Solanum Tuberosum L.), Nehal Dubey, Maitri Trivedi, Suresh Varsani, Vishal Vyas, Manisha Farsodia, Sunil Kumar Singh Jan 2019

Genome-Wide Characterization, Molecular Evolution And Mexpression Profiling Of The Metacaspases In Potato (Solanum Tuberosum L.), Nehal Dubey, Maitri Trivedi, Suresh Varsani, Vishal Vyas, Manisha Farsodia, Sunil Kumar Singh

Faculty Publications: Department of Entomology

Metacaspases are distant relatives of animal caspases found in plants, protozoa and fungi. Some recent studies have demonstrated that metacaspases are involved in regulating the developmental and environmentally induced programmed cell death in plants. In this study, we identified metacaspase gene family in potato (Solanum tuberosum L.) and analyzed their expression pattern in various developmental tissues and stress responses of plants. There were eight metacaspase genes identified in the Peptidase (Cysteine protease) C14 family and based upon sequence alignment and phylogenetic analysis, a systematic nomenclature of potato metacaspases (SotubMCs) has been proposed. Three of the eight candidate genes showing homology ...