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Full-Text Articles in Life Sciences

Detection Of Heteroplasmic Single Nucleotide Polymorphisms Using Melt Curve Analysis And Dual Labeled Fluorescent Probes, Emily Jezewski Apr 2019

Detection Of Heteroplasmic Single Nucleotide Polymorphisms Using Melt Curve Analysis And Dual Labeled Fluorescent Probes, Emily Jezewski

Honors Theses, University of Nebraska-Lincoln

Plant mitochondrial genomes are strange – they are unusually large, consist of huge amounts of non-coding DNA, and contain of several overlapping regions throughout the genome. The genome is made of several different sized linear and circular molecules and different mitochondria within a cell will have different pieces of the genome. Even if multiple mitochondria contain the same region of the genome, these sequences can differ by single nucleotide polymorphisms (SNPS). This is known as heteroplasmy. Heteroplasmy has been documented in Arabidopsis thaliana using whole-genome sequencing data. While heteroplasmy is well-documented, its existence in the mitochondrial genome is unexpected and it ...


H/L Ratio As A Measurement Of Immune Function In Terrapene Ornata Species, Vesa Govori Mar 2019

H/L Ratio As A Measurement Of Immune Function In Terrapene Ornata Species, Vesa Govori

Honors Theses, University of Nebraska-Lincoln

White blood cells are a key part of the immune system, and changes in white cell count can reveal the overall state of health and the immune systems functional ability of a species. White blood such as heterophils and lymphocytes are essential for being able to fight off infections. Analyzing different cell ratios has been commonly done before as a way to observe immune function in bird species as well as other species. Understanding that there are different functions between the different white blood cells provides an extra interesting perspective to what could be happening in the immune system function ...


No More, Monsanto. A Comprehensive Review Of The Most Hated Agriculture/Biotechnology/Chemical/Confectionery/Pharmaceutical Company In America., Gabrielle Robbins Mar 2019

No More, Monsanto. A Comprehensive Review Of The Most Hated Agriculture/Biotechnology/Chemical/Confectionery/Pharmaceutical Company In America., Gabrielle Robbins

Honors Theses, University of Nebraska-Lincoln

Since 1901, Monsanto's roots have held strong to its grip as a conglomerate in chemical, agriculture, and biotechnology industries. This creative project will deconstruct, investigate and strategically interpret the brand's history, logo, social responsibility and so much more of the good, bad and the ugly.


Investigating The Role Of Free Radicals In Huntington's Disease Using Drosophila Melanogaster, Jennifer Libov Mar 2019

Investigating The Role Of Free Radicals In Huntington's Disease Using Drosophila Melanogaster, Jennifer Libov

Honors Theses, University of Nebraska-Lincoln

During normal cell metabolism, reactive oxygen species (ROS) are produced as a byproduct of oxidative phosphorylation. ROS are utilized in the cell as a signaling molecule and can be maintained at healthy levels by cellular antioxidants. However, when the cell experiences oxidative stress due to environmental or genetic conditions, levels of ROS can exceed healthy levels and inhibit necessary life functions by damaging biomolecules and cellular structures. This loss of function can lead to physiological decline and neurodegeneration, such as in diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and, potentially, Huntington’s disease. The following experiments use the model ...


A Comparative Analysis Of The Fermentation Capabilities Of Various Bifidobacterium Strains, Ella Oney Mar 2019

A Comparative Analysis Of The Fermentation Capabilities Of Various Bifidobacterium Strains, Ella Oney

Honors Theses, University of Nebraska-Lincoln

Bifidobacterium is a genus of anaerobic bacteria that are commonly found to inhabit the gastrointestinal tract of many members of the animal kingdom. These microorganisms are adapted to obtain their carbon from the breakdown of complex carbohydrates. Marmosets, a mammal whose gut microbiome is inhabited by high levels of Bifidobacteria, consume gum Arabic as a major part of their diet. The purpose of this experiment is to determine whether Bifidobacterium strains isolated from the guts of marmosets are able to degrade and ferment this complex carbohydrate or one of its main constituents, arabinose. This was accomplished by inoculating isolates of ...


Escaping Death: Naloxone's Chemical Nature And Potential To Combat The Opioid Epidemic, Abigale Miller Mar 2019

Escaping Death: Naloxone's Chemical Nature And Potential To Combat The Opioid Epidemic, Abigale Miller

Honors Theses, University of Nebraska-Lincoln

Naloxone is a life-saving drug with the ability to reverse an opioid overdose. As the opioid epidemic’s death toll rises, we can turn to Naloxone as a tool to combat the crisis. The epidemic, born of corruption, has a wide reach among the people of the United States, with especially firm grasps on middle-aged people, sufferers of chronic pain, white Americans and those living in the eastern portion of the country. Naloxone’s elegant design saves lives by effectively competing for a position on an opioid biding receptor in the brain to almost instantly end an overdose and restore ...


Dietary Polyphenols And Their Perceived Health Benefits, Stephanie Russell Mar 2019

Dietary Polyphenols And Their Perceived Health Benefits, Stephanie Russell

Honors Theses, University of Nebraska-Lincoln

This paper will consist of a compilation of information on polyphenol classes, dietary sources, and potential health benefits. Polyphenols can be classified as flavonoids, phenolic acids, lignans, or stilbenes. These molecules consist of over 500 plant metabolites that come from numerous food sources including various fruits, vegetables, grains, and beverages such as coffee, tea, and wine. The quantity of polyphenols in food vary greatly, as does their bioavailability for use in the body after consumption. Due to antioxidant and anti-inflammatory properties, polyphenols are being studied for effects on reducing the risk of cardiovascular disease. This paper will focus on structural ...


Student Perceptions Of Reasons For Lecture And Active Learning, Calan Koch Mar 2019

Student Perceptions Of Reasons For Lecture And Active Learning, Calan Koch

Honors Theses, University of Nebraska-Lincoln

With perceived student resistance to active learning in the classroom, instructors are hesitant to implement such methods into their classroom structure despite how effective they may be. This research seeks to understand student perceptions related to the transition to higher prevalence of active learning techniques. We aimed to find answers to how students perceive the ideal classroom to be structured, what reasons they perceive for lecture and active learning components, and possible explanations to concerns of groupwork during class time. We analyzed 64 interviews with undergraduate biology students at the University of Nebraska- Lincoln, developed coding rubrics from commonly found ...


Treatment Of Plastic Wastes Using Plasma Gasification Technology, Zachary Homolka Jan 2019

Treatment Of Plastic Wastes Using Plasma Gasification Technology, Zachary Homolka

Honors Theses, University of Nebraska-Lincoln

Plasma gasification (PG) complements traditional recycling when applied to contaminated or mixed plastics. Without PG these plastics cost recyclers more to process than they are worth on the market, and sometimes they are landfilled or incinerated instead of being recycled. Plasma gasification can take plastic not suitable for traditional recycling and break it down into high-quality syngas for use in electricity generation, chemical manufacturing, or hydrogen production. The technology can be implemented without changing the behavior of consumers, which is a major advantage over attempting to decrease contamination or reduce use of low-value plastic. Due to high capital requirements and ...