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Articles 1 - 9 of 9

Full-Text Articles in Life Sciences

Metabolism Underlies Physiological Homeostasis In Drosophila, Omera B. Matoo, Cole R. Julick, Kristi Montooth Jun 2019

Metabolism Underlies Physiological Homeostasis In Drosophila, Omera B. Matoo, Cole R. Julick, Kristi Montooth

Faculty Publications in the Biological Sciences

Organismal physiology emerges from metabolic pathways and subcellular structures like the mitochondria that can vary across development and among individuals. Here, we tested whether genetic variation at one level of physiology can be buffered at higher levels of biological organization during development by the inherent capacity for homeostasis in physiological systems. We found that the fundamental scaling relationship between mass and metabolic rate, as well as the oxidative capacity per mitochondria, changed significantly across development in the fruit fly Drosophila. However, mitochondrial respiration rate was maintained at similar levels across development. Furthermore, larvae clustered into two types—those that switched ...


Life History Traits And Functional Processes Generate Multiple Pathways To Ecological Stability, John P. Delong, Torrance C. Hanley, Jean P. Gibert, Linda M. Puth, David M. Post Apr 2019

Life History Traits And Functional Processes Generate Multiple Pathways To Ecological Stability, John P. Delong, Torrance C. Hanley, Jean P. Gibert, Linda M. Puth, David M. Post

Faculty Publications in the Biological Sciences

Stability contributes to the persistence of ecological communities, yet the interactions among different stabilizing forces are poorly understood. We assembled mesocosms with an algal resource and one to eight different clones of the consumer Daphnia ambigua and tracked algal and Daphnia abundances through time. We then fitted coupled ordinary differential equations (ODEs) to the consumer–resource time series. We show that variation in different components of stability (local stability and the magnitude of population fluctuations) across mesocosms arises through variation in life history traits and the functional processes represented by ODE model parameters. Local stability was enhanced by increased algal ...


Morphological Changes In American Kestrels (Falco Sparverius) At Continental Migration Sites, Teresa E. Ely, Christopher W. Briggs, Shawn E. Hawks, Gregory S. Kaltenecker, David L. Evans, Frank J. Nicoletti, Jean-François Therrien, Olin Allen, John Delong Apr 2019

Morphological Changes In American Kestrels (Falco Sparverius) At Continental Migration Sites, Teresa E. Ely, Christopher W. Briggs, Shawn E. Hawks, Gregory S. Kaltenecker, David L. Evans, Frank J. Nicoletti, Jean-François Therrien, Olin Allen, John Delong

Faculty Publications in the Biological Sciences

Many American kestrel (Falco sparverius) populations are declining across North America. Potential causes include mortality from reduction in food availability, a changing climate, habitat degradation, an increase in avian predators, disease, and toxins. We analyzed American kestrel count and banding data from seven raptor migration sites throughout North America with at least 20 years of migration data. We used count data to determine the year at which the kestrel population began a significant decline and then used banding records to determine whether body mass and wing chord declined after this point. We found reductions in kestrel body mass at three ...


Enzyme Kinetics Of Dutpase From The Planarian Dugesia Ryukyuensis, Shahanoor Alam, Hideaki Moriyama Mar 2019

Enzyme Kinetics Of Dutpase From The Planarian Dugesia Ryukyuensis, Shahanoor Alam, Hideaki Moriyama

Faculty Publications in the Biological Sciences

Objective: Planarians including Dugesia ryukyuensis (Dr) have strong regenerative abilities that require enhanced DNA replication. Knockdown of the DUT gene in Dr, which encodes deoxyuridine 5′-triphosphate pyrophosphatase (dUTPase), promotes DNA fragmentation, inhibits regeneration, and eventually leads to death. dUTPase catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate. dUTPase is known to prevent uracil misincorporation in DNA by balancing the intracellular ratio between dUTP and dTTP, and contributes to genome stability. Nevertheless, the catalytic performance of Dr-dUTPase has not been reported.

Results: To confirm the catalytic activity of Dr-dUTPase, we cloned and expressed Dr-DUT in E. coli. Then, we ...


Microbial Community Of Saline, Alkaline Lakes In The Nebraska Sandhills Based On 16s Rrna Gene Amplicon Sequence Data, Nicole A. Fiore, David D. Dunigan Ph. D., Julie J. Shaffer, Ryan Roberts, Sanjay Antony-Babu, Bradley A. Plantz, Kenneth W. Nickerson, Andrew K. Benson, Karrie A. Weber Mar 2019

Microbial Community Of Saline, Alkaline Lakes In The Nebraska Sandhills Based On 16s Rrna Gene Amplicon Sequence Data, Nicole A. Fiore, David D. Dunigan Ph. D., Julie J. Shaffer, Ryan Roberts, Sanjay Antony-Babu, Bradley A. Plantz, Kenneth W. Nickerson, Andrew K. Benson, Karrie A. Weber

Faculty Publications in the Biological Sciences

The Nebraska Sandhills region contains over 1,500 geochemically diverse interdunal lakes, some of which are potassium rich, alkaline, and hypersaline. Here, we report 16S rRNA amplicon pyrosequencing data on the water and sediment microbial communities of eight alkaline lakes in the Sandhills of western Nebraska.


Persistent Viral Reservoirs In Lymphoid Tissues In Siv-Infected Rhesus Macaques Of Chinese-Origin On Suppressive Antiretroviral Therapy, Summer Siddiqui, Stefanie Perez, Yong Gao, Lara Doyle-Meyers, Brian T. Foley, Qingsheng Li, Binhua Ling Jan 2019

Persistent Viral Reservoirs In Lymphoid Tissues In Siv-Infected Rhesus Macaques Of Chinese-Origin On Suppressive Antiretroviral Therapy, Summer Siddiqui, Stefanie Perez, Yong Gao, Lara Doyle-Meyers, Brian T. Foley, Qingsheng Li, Binhua Ling

Faculty Publications in the Biological Sciences

Understanding HIV latent reservoirs in tissues is essential for the development of new strategies targeting these sites for eradication. Here, we assessed the size of latent reservoirs and the source of residual viruses in multiple lymphoid tissues of SIV-infected and fully suppressed rhesus macaques of Chinese-origin (cRMs). Eight cRMs were infected with SIVmac251 and treated with tenofovir and emtricitabine daily for 24 weeks initiated 4 weeks post-infection. Four of the eight animals reached sustained full viral suppression with undetectable viremia. The levels of cell-associated SIV DNA varied in peripheral blood mononuclear cells (PBMCs) and multiple lymphoid tissues, but with higher ...


Can Vegfc Form Turing Patterns In The Zebrafish Embyro?, Kenneth Y. Wertheim, Tina Roose Jan 2019

Can Vegfc Form Turing Patterns In The Zebrafish Embyro?, Kenneth Y. Wertheim, Tina Roose

Faculty Publications in the Biological Sciences

This paper is concerned with a late stage of lymphangiogenesis in the trunk of the zebrafish embryo. At 48 hours pos-fertilisation (HPF), a pool of parachordal lymphangioblasts (PLs) lies in the horizontal myoseptum. Between 48 and 168 HPF, the PLs spread from the horizontal myoseptum to form the throacic duct, dorsal longitudinal lymphatic vessel, and parachordal lymphatic vessel. This paper deals wit hthe potential of vascular endothelial growth factor C (VEGFC) to guide the differentiation of PLs into the mature lymphatic endothelial cells that form the vessels. We built a mathematical model to describe the biochemical interactions between VEGFC, collagen ...


Radiomic Feature Stability Across 4d Respiratory Phases And Its Impact On Lung Tumor Prognosis Prediction, Qian Du, Michael Baine, Kyle Bavitz, Josiah Mcallister, Xiaoying Liang, Hongfeng Yu, Jeffrey Ryckman, Lina Yu, Hengle Jiang, Sumin Zhou, Sumin Zhou, Chi Zhang, Dandan Zheng Jan 2019

Radiomic Feature Stability Across 4d Respiratory Phases And Its Impact On Lung Tumor Prognosis Prediction, Qian Du, Michael Baine, Kyle Bavitz, Josiah Mcallister, Xiaoying Liang, Hongfeng Yu, Jeffrey Ryckman, Lina Yu, Hengle Jiang, Sumin Zhou, Sumin Zhou, Chi Zhang, Dandan Zheng

Faculty Publications in the Biological Sciences

Radiomic analysis has recently demonstrated versatile uses in improving diagnostic and prognostic prediction accuracy for lung cancer. However, since lung tumors are subject to substantial motion due to respiration, the stability of radiomic features over the respiratory cycle of the patient needs to be investigated to better evaluate the robustness of the interpatient feature variability for clinical applications, and its impact in such applications needs to be assessed. A full panel of 841 radiomic features, including tumor intensity, shape, texture, and wavelet features, were extracted from individual phases of a four-dimensional (4D) computed tomography on 20 early-stage non-small-cell lung cancer ...


Ecological Pleiotropy And Indirect Effects Alter The Potential For Evolutionary Rescue, John P. Delong, Jonathan Belmaker Jan 2019

Ecological Pleiotropy And Indirect Effects Alter The Potential For Evolutionary Rescue, John P. Delong, Jonathan Belmaker

Faculty Publications in the Biological Sciences

Invading predators can negatively affect naïve prey populations due to a lack of evolved defenses. Many species therefore may be at risk of extinction due to overexploitation by exotic predators. Yet the strong selective effect of predation might drive evolution of imperiled prey toward more resistant forms, potentially allowing the prey to persist. We evaluated the potential for evolutionary rescue in an imperiled prey using Gillespie eco‐evolutionary models (GEMs). We focused on a system parameterized for protists where changes in prey body size may influence intrinsic rate of population growth, space clearance rate (initial slope of the functional response ...