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Articles 1 - 11 of 11

Full-Text Articles in Life Sciences

Predation On Stink Bugs (Hemiptera: Pentatomidae) In Cotton And Soybean Agroecosystems, Kacie J. Athey, John R. Ruberson, Dawn M. Olson, James D. Harwood Mar 2019

Predation On Stink Bugs (Hemiptera: Pentatomidae) In Cotton And Soybean Agroecosystems, Kacie J. Athey, John R. Ruberson, Dawn M. Olson, James D. Harwood

Faculty Publications: Department of Entomology

Stink bugs (Hemiptera: Pentatomidae) are significant pests of cotton and soybeans in the southeastern United States with annual control costs exceeding $14 million in these crops. Three of the most prominent stink bug pests are the southern green (Nezara viridula), brown (Euschistus servus) and green (Chinavia hilaris) stink bugs. To determine trophic linkages between generalist arthropod predators and these pests, species-specific 16S molecular markers were designed and used to detect the presence of prey DNA in predator gut-contents. Over 2700 predators were collected over two growing seasons in cotton and soybean in southern Georgia in 2011 and 2012 and screened ...


Global Responses Of Resistant And Susceptible Sorghum (Sorghum Bicolor) To Sugarcane Aphid (Melanaphis Sacchari), Hannah M. Tetreault, Sajjan Grover, Erin Scully, Tammy Gries, Nathan A. Palmer, Gautam Sarath, Joe Louis, Scott E. Sattler Feb 2019

Global Responses Of Resistant And Susceptible Sorghum (Sorghum Bicolor) To Sugarcane Aphid (Melanaphis Sacchari), Hannah M. Tetreault, Sajjan Grover, Erin Scully, Tammy Gries, Nathan A. Palmer, Gautam Sarath, Joe Louis, Scott E. Sattler

Faculty Publications: Department of Entomology

The sugarcane aphid (Melanaphis sacchari) has emerged as a significant pest for sorghum. The use of sugarcane aphid-resistant sorghum germplasm with integrated pest management strategies appears to be an excellent solution to this problem. In this study, a resistant line (RTx2783) and a susceptible line (A/BCK60) were used to characterize the differences in plant responses to the sugarcane aphid through a series of experiments, which examined global sorghum gene expression, aphid feeding behavior and inheritance of aphid resistance. The global transcriptomic responses to sugarcane aphids in resistant and susceptible plants were identified using RNA-seq and compared to the expression ...


Effects Of An Unregistered Insecticide On Adult Spotted Wing Drosophila Mortality And Field Infestation Rates, 2015, Katharine A. Swoboda-Bhattarai, Hannah Burrack Feb 2019

Effects Of An Unregistered Insecticide On Adult Spotted Wing Drosophila Mortality And Field Infestation Rates, 2015, Katharine A. Swoboda-Bhattarai, Hannah Burrack

Faculty Publications: Department of Entomology

The effectiveness of an unregistered material at reducing Drosophila suzukii (SWD) infestation rates in blackberry field plots and its toxicity to adult SWD in laboratory bioassays was tested during the 2015 growing season. Field trials were conducted at the Sandhills Research Station near Jackson Springs, NC, in a 0.11-acre planting consisting of two rows of ‘Ouachita’ blackberries. Six treatments, including an untreated check (UTC), were applied to 5-plant plots and were arranged in an RCB design with four replicates per treatment, blocked by row, with two blocks per row. Delegate was the standard material. Some of the treatments were ...


Genome-Wide Characterization, Molecular Evolution And Mexpression Profiling Of The Metacaspases In Potato (Solanum Tuberosum L.), Nehal Dubey, Maitri Trivedi, Suresh Varsani, Vishal Vyas, Manisha Farsodia, Sunil Kumar Singh Jan 2019

Genome-Wide Characterization, Molecular Evolution And Mexpression Profiling Of The Metacaspases In Potato (Solanum Tuberosum L.), Nehal Dubey, Maitri Trivedi, Suresh Varsani, Vishal Vyas, Manisha Farsodia, Sunil Kumar Singh

Faculty Publications: Department of Entomology

Metacaspases are distant relatives of animal caspases found in plants, protozoa and fungi. Some recent studies have demonstrated that metacaspases are involved in regulating the developmental and environmentally induced programmed cell death in plants. In this study, we identified metacaspase gene family in potato (Solanum tuberosum L.) and analyzed their expression pattern in various developmental tissues and stress responses of plants. There were eight metacaspase genes identified in the Peptidase (Cysteine protease) C14 family and based upon sequence alignment and phylogenetic analysis, a systematic nomenclature of potato metacaspases (SotubMCs) has been proposed. Three of the eight candidate genes showing homology ...


Differential Spatial Gradients Of Wheat Streak Mosaic Virus Into Winter Wheat From A Central Mite-Virus Source, Abby R. Stilwell, Donald Rundquist, David B. Marx, Gary L. Hein Jan 2019

Differential Spatial Gradients Of Wheat Streak Mosaic Virus Into Winter Wheat From A Central Mite-Virus Source, Abby R. Stilwell, Donald Rundquist, David B. Marx, Gary L. Hein

Faculty Publications: Department of Entomology

The wheat curl mite (WCM), Aceria tosichella Keifer, transmits three potentially devastating viruses to winter wheat. An increased understanding of mite movement and subsequent virus spread through the landscape is necessary to estimate the risk of epidemics by the virus in winter wheat. Owing to the small size of WCMs, their dispersal via wind is hard to monitor; however, the viruses they transmit produce symptoms that can be detected with remote sensing. The objective of this study was to characterize the spatial dispersal of the virus from a central mite-virus source. Virus infection gradients were measured spatially by using aerial ...


Native Solitary Bees Provide Economically Significant Pollination Services To Confection Sunflowers (Helianthus Annuus L.) (Asterales: Asteraceae) Grown Across The Northern Great Plains, Rachel E. Mallinger, Jeff Bradshaw, Adam J. Varenhorst, Jarrad R. Prasifka Jan 2019

Native Solitary Bees Provide Economically Significant Pollination Services To Confection Sunflowers (Helianthus Annuus L.) (Asterales: Asteraceae) Grown Across The Northern Great Plains, Rachel E. Mallinger, Jeff Bradshaw, Adam J. Varenhorst, Jarrad R. Prasifka

Faculty Publications: Department of Entomology

The benefits of insect pollination to crop yields depend on genetic and environmental factors including plant selffertility, pollinator visitation rates, and pollinator efficacy. While many crops benefit from insect pollination, such variation in pollinator benefits across both plant cultivars and growing regions is not well documented. In this study, across three states in the northern Great Plains, United States, from 2016 to 2017, we evaluated the pollinatormediated yield increases for 10 varieties of confection sunflowers, Helianthus annuus L. (Asterales: Asteraceae), a plant that is naturally pollinator-dependent but was bred for self-fertility. We additionally measured pollinator visitation rates and compared per-visit ...


Advances In Molecular Research On Bed Bugs (Hemiptera: Cimicidae), Sanjay Basnet, Shripat T. Kamble Jan 2019

Advances In Molecular Research On Bed Bugs (Hemiptera: Cimicidae), Sanjay Basnet, Shripat T. Kamble

Faculty Publications: Department of Entomology

With the resurgence and increase in infestations of the common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), across the world, there has been renewed interest in molecular research on this pest. In this paper, we present current information on the biology, medical importance, management practices, behavior and physiology, and molecular research conducted on bed bugs. The majority of molecular studies are focused towards understanding the molecular mechanism of insecticide resistance. Bed bugs are hematophagous insects with no prior record of vectoring any disease organisms. An improved understanding of how bed bugs lack vector competency may provide information to prevent disease ...


Rnai Targeting Of Rootworm Troponin I Transcripts Confers Root Protection In Maize, Elane Fishilevich, Andrew J. Bowling, Meghan L. F. Frey, Po-Hao Wang, Wendy Lo, Murugesan Rangasamy, Sarah E. Worden, Heather E. Pence, Premchand Gandra, Shannon L. Whitlock, Greg Schulenberg, Eileen Knorr, Linda Tenbusch, Jamie R. Lutz, Stephen Novak, Ronda L. Hamm, Karl D. Schnelle, Andreas Vilcinskas, Kenneth E. Narva Jan 2019

Rnai Targeting Of Rootworm Troponin I Transcripts Confers Root Protection In Maize, Elane Fishilevich, Andrew J. Bowling, Meghan L. F. Frey, Po-Hao Wang, Wendy Lo, Murugesan Rangasamy, Sarah E. Worden, Heather E. Pence, Premchand Gandra, Shannon L. Whitlock, Greg Schulenberg, Eileen Knorr, Linda Tenbusch, Jamie R. Lutz, Stephen Novak, Ronda L. Hamm, Karl D. Schnelle, Andreas Vilcinskas, Kenneth E. Narva

Faculty Publications: Department of Entomology

Western corn rootworm, Diabrotica virgifera virgifera, is the major agronomically important pest of maize in the US Corn Belt. To augment the repertoire of the available dsRNA-based traits that control rootworm, we explored a potentially haplolethal gene target, wings up A (wupA), which encodes Troponin I. Troponin I, a component of the Troponin-Tropomyosin complex, is an inhibitory protein involved in muscle contraction. In situ hybridization showed that feeding on wupA-targeted dsRNAs caused systemic transcript knockdown in D. v. virgifera larvae. The knockdown of wupA transcript, and by extension Troponin I protein, led to deterioration of the striated banding pattern ...


Opda Regulates Maize Defense Against Aphids, Suresh Varsani, Sajjan Grover, Shaoqun Zhou, Kyle G. Koch, Pei-Cheng Huang, Michael V. Kolomiets, W. Paul Williams, Tiffany Heng-Moss, Gautam Sarath, Dawn S. Luthe, Georg Jander, Joe Louis Jan 2019

Opda Regulates Maize Defense Against Aphids, Suresh Varsani, Sajjan Grover, Shaoqun Zhou, Kyle G. Koch, Pei-Cheng Huang, Michael V. Kolomiets, W. Paul Williams, Tiffany Heng-Moss, Gautam Sarath, Dawn S. Luthe, Georg Jander, Joe Louis

Faculty Publications: Department of Entomology

Copyright © 2019 American Society of Plant Biologists.

DOI:10.1104/pp.18.01472


Effects Of Diet Quality And Temperature On Stable Fly (Diptera: Muscidae) Development, Melina Florez-Cuadros, Dennis Berkebile, Gary Brewer Jan 2019

Effects Of Diet Quality And Temperature On Stable Fly (Diptera: Muscidae) Development, Melina Florez-Cuadros, Dennis Berkebile, Gary Brewer

Faculty Publications: Department of Entomology

The effects of diet quality and temperature on the development time and size of stable flies, Stomoxys calcitrans (L.), was evaluated. Both development time and size varied relative to diet quality and temperature, and their effects were additive. Diet quality and temperature made similar contributions to the variance in size whereas temperature was responsible for >97% of the variance in development time. Regression analysis predicted the shortest development time, egg to adult, to be 12.7 days at 32 C and 70% nutrients. Egg to adult development varied curvilinearly relative to diet quality and temperature on the degree day 10 ...


Evaluation Of Reference Genes For Real-Time Quantitative Pcr Analysis In Southern Corn Rootworm, Diabrotica Undecimpunctata Howardi (Barber), Saumik Basu, Adriano E. Pereira, Daniele H. Pinheiro, Haichuan Wang, Arnubio Valencia-Jimenez, Blair D. Siegfried, Joe Louis, Xuguo 'Joe' Zhou, Ana Maria Velez Jan 2019

Evaluation Of Reference Genes For Real-Time Quantitative Pcr Analysis In Southern Corn Rootworm, Diabrotica Undecimpunctata Howardi (Barber), Saumik Basu, Adriano E. Pereira, Daniele H. Pinheiro, Haichuan Wang, Arnubio Valencia-Jimenez, Blair D. Siegfried, Joe Louis, Xuguo 'Joe' Zhou, Ana Maria Velez

Faculty Publications: Department of Entomology

Quantitative reverse transcription PCR (RT-qPCR) is one of the most efficient, reliable and widely used techniques to quantify gene expression. In this study, we evaluated the performance of six southern corn rootworm, Diabrotica undecimpunctata howardi (Barber), housekeeping genes (HKG), β-actin (Actin), β-tubulin (Tubulin), elongation factor 1 alpha (EF1α), glyceraldehyde-3 phosphate dehydrogenase (GAPDH), 40 S ribosomal protein S9 (RpS9) and ubiquitin-conjugating protein (Ubi), under different experimental conditions such as developmental stage, exposure of neonate and adults to dsRNA, exposure of adults to different temperatures, different 3rd instar larva tissues, and neonate starvation. The HKGs were analyzed with four algorithms ...