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Canavalia And Dolichos Extracts For Sustainable Pest Biocontrol And Plant Nutrition Improvement In El Salvador, Carlos Martinez Oct 2019

Canavalia And Dolichos Extracts For Sustainable Pest Biocontrol And Plant Nutrition Improvement In El Salvador, Carlos Martinez

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Botanical repellents and pesticides are now being rediscovered as new tools for integrated pest management in order to reduce the use of toxic chemicals in crop production. Canavalia gladiata and Dolichos lablab are two Fabaceae very well adapted to farmlands of El Salvador, effective as living barriers and mostly as cover crops, however, they are not yet very well disseminated. This document describes the potential for using the liquid extracts and the dry flour of raw seeds of those plants for economic benefit and practical convenience for pest management in Salvadorian agriculture under field conditions. Seed extracts were useful when ...


Rust And Viral Mosaic Diseases In Biofuel Switchgrass, Anthony A. Muhle Aug 2019

Rust And Viral Mosaic Diseases In Biofuel Switchgrass, Anthony A. Muhle

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Switchgrass (Panicum virgatum L.) is a perennial warm-season monocot that is indigenous to locations in North America east of the Rocky Mountains, and is considered a model grass for biofuel feedstock production. As switchgrass production increases, diseases pose a potential threat to biomass production and ethanol extraction. The two predominant switchgrass diseases in Nebraska are rust caused by Puccinia spp. and a viral mosaic disease caused by Panicum mosaic virus (PMV) and its associated Satellite panicum mosaic virus (SPMV). In this thesis, one study determined how SPMV affects PMV infection and systemic spread in two populations of switchgrass at different ...


An Integrated Genomics And Phenomics Approach To Study The Evolution Of C4 Photosynthesis, Daniel Santana De Carvalho Aug 2019

An Integrated Genomics And Phenomics Approach To Study The Evolution Of C4 Photosynthesis, Daniel Santana De Carvalho

Theses, Dissertations, and Student Research in Agronomy and Horticulture

The C4 photosynthetic pathway was first described over 50 years ago. Today, it is known that C4 evolved independently > 60 in plant lineages, which involves understanding not only the genetic, but also the metabolic features and differences involved in this process. Also, several adaptations are involved in the evolution of this type of photosynthesis, for example: changes in leaf anatomy and the evolution of kranz anatomy, physiology and metabolic pathways. In order to further investigate this pathway, different technologies and methods have been developed to unravel genes involved in C4 photosynthesis. With the advances in molecular biology and bioinformatics tools ...


Hormonal Signaling Induced In Soybean By Lysobacter Enzymogenes Strain C3, Jessica C. Walnut Aug 2019

Hormonal Signaling Induced In Soybean By Lysobacter Enzymogenes Strain C3, Jessica C. Walnut

Theses, Dissertations, and Student Research in Agronomy and Horticulture

The biological control bacterium Lysobacter enzymogenes strain C3 has been shown to suppress fungal diseases by producing a suite of lytic enzymes and antimicrobial secondary metabolites. Previous studies have found that C3, when applied to grass and cereal plants, also is capable of inducing local and systemic resistance against fungal pathogens. It is unknown, however, whether the bacterium has the ability to induce resistance in dicots and what signaling pathways are involved. This study assessed the ability of C3 to trigger local and systemic induced resistance responses in soybean (Glycine max ‘Williams82’) by analyzing relative expression of salicylic acid (SA ...


Introduction To Sorghum Paper Production, Zachary Christman May 2019

Introduction To Sorghum Paper Production, Zachary Christman

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Sorghum is a tall grass used for many commercial products such as fodder and syrup. The 10 to 15 feet stalk of the plant has lower lignin than wood and provides a fiber length of 2.31 mm in the outer covering and 1.38 mm for the leaf. Sorghum fiber makes a high quality, strong paper suitable for printing, packaging and paperboard.


Use Of Annual Forage Mixtures In Crop/Livestock Production Systems In Nebraska, Nathan Paul Pflueger May 2019

Use Of Annual Forage Mixtures In Crop/Livestock Production Systems In Nebraska, Nathan Paul Pflueger

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Success of integrating annual forages into crop and livestock systems throughout Nebraska may be variable depending on field location, field/forage crop management, and precipitation. There are many different warm- and cool-season annual forage species available for integrating crop and livestock systems at different times of the year. Mixtures of cereal species, such as oats (Avena sativa)) and spring peas (Pisum sativum)), are often used to optimize forage quantity and forage quality. Our two-year, three location study across Nebraska’s precipitation gradient suggested that forage quantity and quality may vary by location due to different precipitation amounts received during the ...


Establishment Of Perennial Legumes With An Annual Warm-Season Grass As A Companion Crop, Martina N. La Vallie May 2019

Establishment Of Perennial Legumes With An Annual Warm-Season Grass As A Companion Crop, Martina N. La Vallie

Theses, Dissertations, and Student Research in Agronomy and Horticulture

The yields of perennial forage legumes are often hindered during the establishment year due to slow germination rates and weed competition. This study was conducted to determine if sorghum-sudangrass (Sorghum bicolor x S. bicolor var. sudanese) is a compatible annual companion crop for increased forage production, weed suppression, and legume establishment. In 2016, sorghum-sudangrass was paired with alfalfa (Medicago sativa L. ‘Ranger’), birdsfoot trefoil (Lotus corniculatus L.), Illinois bundleflower [Desmanthus illinoensis (Michx.) MacMill. ex B.L. Rob. & Fernald], purple prairie clover (Dalea purpurea Vent.), red clover (Trifolium pratense L.), and roundhead lespedeza (Lespedeza capitata Michx.). We studied effects of a sorghum-sudangrass companion crop with a varying number of harvests (three vs. four harvests) collected per plot throughout the summer and compared yields to the yield of a weeded legume treatment, and a non-weeded legume treatment. In 2017, we studied effects of the application of four seeding rates for sorghum-sudangrass at 5 pure live seed per m2 (PLS/m2), 10 PLS/m2, 20 PLS/m2, and 40 PLS/m2 paired with only the alfalfa perennial legume and compared yields to the yield of an oat-alfalfa control treatment, a weeded alfalfa treatment, and a non-weeded alfalfa treatment. Total dry matter yields along with the yield of each legume, weeds, sorghum-sudangrass, and oats (second year only) were collected for each treatment. In both years, we found the addition of sorghum-sudangrass increased overall dry matter yield and significantly decreased weed abundance. The increase in total dry matter yield came at a cost to the legume yield; as treatments planted with sorghum-sudangrass or oats had lower legume/alfalfa yields than weeded legume/alfalfa treatments. These results suggest that sorghum-sudangrass is ...


Dna Barcoding Of Pratylenchus From Agroecosystems In The Northern Great Plains Of North America, Mehmet Ozbayrak May 2019

Dna Barcoding Of Pratylenchus From Agroecosystems In The Northern Great Plains Of North America, Mehmet Ozbayrak

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Pratylenchus species are among the most common plant parasitic nematodes in the Great Plains Region. The objectives of this study were to barcode Pratylenchus specimens for species identification in the Great Plains region using mitochondrial CO1 DNA barcode. In order to (1) determine species boundaries, (2) assess the host associations of barcoded Pratylenchus, (3) to determine the distribution patterns across the Great Plains Region and, (4) to evaluate the species status of P. scribneri and P. hexincisus by a multivariate morphological analysis of haplotype groups identified by DNA barcoding. Soil samples, primarily associated with eight major crops, were collected from ...


Genome-Wide Association Studies In Maize And Sorghum, Preston Hurst Apr 2019

Genome-Wide Association Studies In Maize And Sorghum, Preston Hurst

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Genome-wide association studies are used to identify genetic variants associated with a particular phenotype. GWAS has been used in a variety of taxa, from humans, to fish to plants . The present analysis is focused on two species important to the human species: maize and sorghum. A GWAS in maize was carried out on the modification of the Ga1-s allele. The Ga1 locus has long been studied as being involved in a unilateral crossing barrier . However, it has long been suspected that the locus is modified by background genetic factors . GWAS was used to observe candidates for this modification. A series ...


Development Of A Nitrogen Recommendation Tool For Corn Considering Static And Dynamic Variables, Laila A. Puntel, Agustin Pagani, Sotirios V. Archontoulis Mar 2019

Development Of A Nitrogen Recommendation Tool For Corn Considering Static And Dynamic Variables, Laila A. Puntel, Agustin Pagani, Sotirios V. Archontoulis

Agronomy & Horticulture -- Faculty Publications

Many soil and weather variables can affect the economical optimum nitrogen (N) rate (EONR) for maize. We classified 54 potential factors as dynamic (change rapidly over time, e.g. soil water) and static (change slowly over time, e.g. soil organic matter) and explored their relative importance on EONR and yield prediction by analyzing a dataset with 51 N trials from Central-West region of Argentina. Across trials, the average EONR was 113 ± 83 kg N ha−1 and the average optimum yield was 12.3 ± 2.2 Mg ha−1, which is roughly 50% higher than the current N rates ...


Global Responses Of Resistant And Susceptible Sorghum (Sorghum Bicolor) To Sugarcane Aphid (Melanaphis Sacchari), Hannah M. Tetreault, Sajjan Grover, Erin Scully, Tammy Gries, Nathan A. Palmer, Gautam Sarath, Joe Louis, Scott E. Sattler Feb 2019

Global Responses Of Resistant And Susceptible Sorghum (Sorghum Bicolor) To Sugarcane Aphid (Melanaphis Sacchari), Hannah M. Tetreault, Sajjan Grover, Erin Scully, Tammy Gries, Nathan A. Palmer, Gautam Sarath, Joe Louis, Scott E. Sattler

Faculty Publications: Department of Entomology

The sugarcane aphid (Melanaphis sacchari) has emerged as a significant pest for sorghum. The use of sugarcane aphid-resistant sorghum germplasm with integrated pest management strategies appears to be an excellent solution to this problem. In this study, a resistant line (RTx2783) and a susceptible line (A/BCK60) were used to characterize the differences in plant responses to the sugarcane aphid through a series of experiments, which examined global sorghum gene expression, aphid feeding behavior and inheritance of aphid resistance. The global transcriptomic responses to sugarcane aphids in resistant and susceptible plants were identified using RNA-seq and compared to the expression ...


Winter Wheat Grain Yield Response To Fungicide Application Is Influenced By Cultivar And Rainfall, Emmanuel Byamukama, Shaukat Ali, Jonathan Kleinjan, Dalitso N. Yabwalo, Christopher Graham, Melanie Caffe-Treml, Nathan D. Mueller, John Rickertsen, William A. Berzonsky Jan 2019

Winter Wheat Grain Yield Response To Fungicide Application Is Influenced By Cultivar And Rainfall, Emmanuel Byamukama, Shaukat Ali, Jonathan Kleinjan, Dalitso N. Yabwalo, Christopher Graham, Melanie Caffe-Treml, Nathan D. Mueller, John Rickertsen, William A. Berzonsky

Agronomy & Horticulture -- Faculty Publications

Winter wheat is susceptible to several fungal pathogens throughout the growing season and foliar fungicide application is one of the strategies used in the management of fungal diseases in winter wheat. However, for fungicides to be profitable, weather conditions conducive to fungal disease development should be present. To determine if winter wheat yield response to fungicide application at the flowering growth stage (Feekes 10.5.1) was related to the growing season precipitation, grain yield from fungicide treated plots was compared to non-treated plots for 19 to 30 hard red winter wheat cultivars planted at 8 site years from 2011 ...


Closing Yield Gaps For Rice Self-Sufficiency In China, Nanyan Deng, Patricio Grassini, Haishun Yang, Jianliang Huang, Kenneth G. Cassman, Shaobing Peng Jan 2019

Closing Yield Gaps For Rice Self-Sufficiency In China, Nanyan Deng, Patricio Grassini, Haishun Yang, Jianliang Huang, Kenneth G. Cassman, Shaobing Peng

Agronomy & Horticulture -- Faculty Publications

China produces 28% of global rice supply and is currently self-sufficient despite a massive rural-to-urban demographic transition that drives intense competition for land and water resources. At issue is whether it will remain self-sufficient, which depends on the potential to raise yields on existing rice land. Here we report a detailed spatial analysis of rice production potential in China and evaluate scenarios to 2030. We find that China is likely to remain self-sufficient in rice assuming current yield and consumption trajectories and no reduction in production area. A focus on increasing yields of double-rice systems on general, and in three ...


Optimum Droplet Size Using A Pulse-Width Modulation Sprayer For Applications Of 2,4-D Choline Plus Glyphosate, Thomas R. Butts, Chase A. Samples, Lucas X. Franca, Darrin M. Dodds, Daniel B. Reynolds, Jason W. Adams, Richard K. Zollinger, Kirk A. Howatt, Bradley K. Fritz, W. Clint Hoffmann, Joe D. Luck, Greg Kruger Jan 2019

Optimum Droplet Size Using A Pulse-Width Modulation Sprayer For Applications Of 2,4-D Choline Plus Glyphosate, Thomas R. Butts, Chase A. Samples, Lucas X. Franca, Darrin M. Dodds, Daniel B. Reynolds, Jason W. Adams, Richard K. Zollinger, Kirk A. Howatt, Bradley K. Fritz, W. Clint Hoffmann, Joe D. Luck, Greg Kruger

Agronomy & Horticulture -- Faculty Publications

The delivery of an optimum herbicide droplet size using pulse-width modulation (PWM) sprayers can reduce potential environmental contamination, maintain efficacy, and provide more flexible options for pesticide applicators. Field research was conducted in 2016, 2017, and 2018 across three locations (Mississippi, Nebraska, and North Dakota) for a total of 6 site-years. The objectives were to evaluate the efficacy of a range of droplet sizes (150 μm [Fine] to 900 μm [Ultra Coarse]) using a 2,4-D choline plus glyphosate pre-mixture and to create novel weed management recommendations using PWM sprayer technology. A pooled site-year generalized additive model explained less than ...


Restoring Fire-Grazer Interactions To Pursue Heterogeneity In Sandhills Prairie, Jack R. Arterburn, Dirac L. Twidwell Jr, Carissa L. Wonkka, Walter H. Schacht, David A. Wedin Jan 2019

Restoring Fire-Grazer Interactions To Pursue Heterogeneity In Sandhills Prairie, Jack R. Arterburn, Dirac L. Twidwell Jr, Carissa L. Wonkka, Walter H. Schacht, David A. Wedin

Agronomy & Horticulture -- Faculty Publications

Heterogeneity has emerged as a fundamental principle for grassland management and the importance of environmental heterogeneity for biological diversity has raised questions about the appropriateness of grassland practices that seek to promote uniform grassland structure and composition. Principles of uniformity in grassland management reflect a utilitarian target of “managing for the middle” by minimizing both overgrazing and underutilization while avoiding or preventing fire and other disturbances that consume aboveground biomass. We evaluated pioneering efforts to restore fire-grazer interactions via patch burning in an effort to increase spatial heterogeneity at the patch scale in the Nebraska Sandhills, a sandy soil, mixed-grass ...


Expression Of The Arabidopsis Wrinkled 1 Transcription Factor Leads To Higher Accumulation Of Palmitate In Soybean Seed, Pamela A. Vogel, Shen Bayon De Noyer, Hyunwoo Park, Hanh Nguyen, Lili Hou, Taity Changa, Hoang Le Khang, Ozan N. Ciftci, Tong Wang, Edgar B. Cahoon, Thomas Elmo Clemente Jan 2019

Expression Of The Arabidopsis Wrinkled 1 Transcription Factor Leads To Higher Accumulation Of Palmitate In Soybean Seed, Pamela A. Vogel, Shen Bayon De Noyer, Hyunwoo Park, Hanh Nguyen, Lili Hou, Taity Changa, Hoang Le Khang, Ozan N. Ciftci, Tong Wang, Edgar B. Cahoon, Thomas Elmo Clemente

Agronomy & Horticulture -- Faculty Publications

Soybean (Glycine max [L.] Merr.) is a commodity crop highly valued for its protein and oil content. The high percentage of polyunsaturated fatty acids in soybean oil results in low oxidative stability, which is a key parameter for usage in baking, high temperature frying applications, and affects shelf life of packaged products containing soybean oil. Introduction of a seed-specific expression cassette carrying the Arabidopsis transcription factor WRINKLED1 (AtWRI1) into soybean, led to seed oil with levels of palmitate up to approximately 20%. Stacking of the AtWRI1 transgenic allele with a transgenic locus harbouring the mangosteen steroyl-ACP thioesterase (GmFatA) resulted in ...


Applicability Of The Dual Isotopes Δ15n And Δ18o To Identify Nitrate In Groundwater Beneath Irrigated Cropland, R. F. Spalding, A. J. Hirsh, M. E. Exner, N. A. Little, K. L. Kloppenborg Jan 2019

Applicability Of The Dual Isotopes Δ15n And Δ18o To Identify Nitrate In Groundwater Beneath Irrigated Cropland, R. F. Spalding, A. J. Hirsh, M. E. Exner, N. A. Little, K. L. Kloppenborg

Agronomy & Horticulture -- Faculty Publications

Identification of the nitrate sources that adversely impact groundwater quality is a necessary first step in the control of this major worldwide pollutant. The impact of nitrate leachate from urea-ammonium nitrate (UAN) (50% urea-N, 25% ammonium-N, 25% nitrate-N) fertilizer, whose use has increased dramatically in the last three decades largely because it can be applied through sprinkler irrigation systems to corn in all growth stages, is investigated. The dual isotopes δ15NNO3 and δ18ONO3 were measured in groundwater samples from 39 irrigation wells in two intensively sprinkler-irrigated, corn-growing areas of Nebraska with nitrate-contaminated (N > 10 ...


Statistical And Machine Learning Methods Evaluated For Incorporating Soil And Weather Into Corn Nitrogen Recommendations, Curtis J. Ransom, Newell R. Kitchen, James J. Camberato, Paul R. Carter, Richard B. Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A. M. Laboski, D. Brenton Myers, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan Jan 2019

Statistical And Machine Learning Methods Evaluated For Incorporating Soil And Weather Into Corn Nitrogen Recommendations, Curtis J. Ransom, Newell R. Kitchen, James J. Camberato, Paul R. Carter, Richard B. Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A. M. Laboski, D. Brenton Myers, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan

Agronomy & Horticulture -- Faculty Publications

Nitrogen (N) fertilizer recommendation tools could be improved for estimating corn (Zea mays L.) N needs by incorporating site-specific soil and weather information. However, an evaluation of analytical methods is needed to determine the success of incorporating this information. The objectives of this research were to evaluate statistical and machine learning (ML) algorithms for utilizing soil and weather information for improving corn N recommendation tools. Eight algorithms [stepwise, ridge regression, least absolute shrinkage and selection operator (Lasso), elastic net regression, principal component regression (PCR), partial least squares regression (PLSR), decision tree, and random forest] were evaluated using a dataset containing ...


Predicting Soil Wind Erosion Potential Under Different Corn Residue Management Scenarios In The Central Great Plains, M. K. Rakkar, Humberto Blanco-Canqui, J. Tatarko Jan 2019

Predicting Soil Wind Erosion Potential Under Different Corn Residue Management Scenarios In The Central Great Plains, M. K. Rakkar, Humberto Blanco-Canqui, J. Tatarko

Agronomy & Horticulture -- Faculty Publications

Various models and simplified equations are available to predict wind erosion potential. However, their performance can be often site-specific, depending on soil characteristics and agronomic practices, warranting sitespecific model validations. Thus, in this study, we 1) validated the wind erodible fraction (WEF) predictive equations by Fryrear et al. (1994) and López et al. (2007) and 2) estimated the total soil loss with the Singleevent Wind Erosion Evaluation Program (SWEEP) using 3-yr measured data from six experiments located across a precipitation gradient in the central Great Plains. Each site had three corn (Zea mays L.) residue removal treatments: control (no removal ...


Ponderosa Pine Regeneration,Wildland Fuels Management, And Habitat Conservation: Identifying Trade-Offs Following Wildfire, Victoria M. Donovan, Caleb P. Roberts, Carissa L. Wonkka, David A. Wedin, Dirac L. Twidwell Jan 2019

Ponderosa Pine Regeneration,Wildland Fuels Management, And Habitat Conservation: Identifying Trade-Offs Following Wildfire, Victoria M. Donovan, Caleb P. Roberts, Carissa L. Wonkka, David A. Wedin, Dirac L. Twidwell

Agronomy & Horticulture -- Faculty Publications

Increasing wildfires in western North American conifer forests have led to debates surrounding the application of post-fire management practices. There is a lack of consensus on whether (and to what extent) post-fire management assists or hinders managers in achieving goals, particularly in under-studied regions like eastern ponderosa pine forests. This makes it difficult for forest managers to balance among competing interests. We contrast structural and community characteristics across unburned ponderosa pine forest, severely burned ponderosa pine forest, and severely burned ponderosa pine forest treated with post-fire management with respect to three management objectives: ponderosa pine regeneration, wildland fuels control, and ...


Beyond Bird Feed: Proso Millet For Human Health And Environment, Saurav Das, Rituraj Khound, Meenakshi Santra, Dipak K. Santra Jan 2019

Beyond Bird Feed: Proso Millet For Human Health And Environment, Saurav Das, Rituraj Khound, Meenakshi Santra, Dipak K. Santra

Agronomy & Horticulture -- Faculty Publications

Domesticated in 8000–10,000 BP in northern China, proso millet (Panicum miliaceum L.) is the best adaptive rotational crop for semiarid central High Plains of the USA, where average annual precipitation is 356–407 mm. Proso millet has multiple benefits when consumed as human food. Proso millet is rich in minerals, dietary fiber, polyphenols, vitamins and proteins. It is gluten-free and therefore, ideal for the gluten intolerant people. Proso millet contains high lecithin which supports the neural health system. It is rich in vitamins (niacin, B-complex vitamins, folic acid), minerals (P, Ca, Zn, Fe) and essential amino acids (methionine ...


A Key Variant In The Cis-Regulatory Element Of Flowering Gene Ghd8 Associated With Cold Tolerance In Rice, Peng Wang, Yin Xiong, Rong Gong, Ying Yang, Kai Fan, Sibin Yu Jan 2019

A Key Variant In The Cis-Regulatory Element Of Flowering Gene Ghd8 Associated With Cold Tolerance In Rice, Peng Wang, Yin Xiong, Rong Gong, Ying Yang, Kai Fan, Sibin Yu

Agronomy & Horticulture -- Faculty Publications

Variations in the gene promoter play critical roles in the evolution of important adaptive traits in crops, but direct links of the regulatory mutation to the adaptive change are not well understood. Here, we examine the nucleotide variations in the promoter region of a transcription factor (Ghd8) that control grain number, plant height and heading date in rice. We find that a dominant promoter type of subspecies japonica displayed a high activity for Ghd8 expression in comparison with the one in indica. Transgenic analyses revealed that higher expression levels of Ghd8 delayed heading date and enhanced cold tolerance in rice ...


A Tmeff2-Regulated Cell Cycle Derived Gene Signature Is Prognostic Of Recurrence Risk In Prostate Cancer, Constantin Georgescu, Joshua M. Corbin, Sandra Thibivilliers, Zachary D. Webb, Yan D. Zhao, Jan Koster, Kar-Ming Fung, Adam S. Asch, Jonathan D. Wren, Maria J. Ruiz-Echevarria Jan 2019

A Tmeff2-Regulated Cell Cycle Derived Gene Signature Is Prognostic Of Recurrence Risk In Prostate Cancer, Constantin Georgescu, Joshua M. Corbin, Sandra Thibivilliers, Zachary D. Webb, Yan D. Zhao, Jan Koster, Kar-Ming Fung, Adam S. Asch, Jonathan D. Wren, Maria J. Ruiz-Echevarria

Agronomy & Horticulture -- Faculty Publications

Background: The clinical behavior of prostate cancer (PCa) is variable, and while the majority of cases remain indolent, 10% of patients progress to deadly forms of the disease. Current clinical predictors used at the time of diagnosis have limitations to accurately establish progression risk. Here we describe the development of a tumor suppressor regulated, cell-cycle gene expression based prognostic signature for PCa, and validate its independent contribution to risk stratification in several radical prostatectomy (RP) patient cohorts.

Methods: We used RNA interference experiments in PCa cell lines to identify a gene expression based gene signature associated with Tmeff2, an androgen ...


Generating High Density, Low Cost Genotype Data In Soybean [Glycine Max (L.) Merr.], Mary M. Happ, Haichuan Wang, George L. Graef, David L. Hyten Jan 2019

Generating High Density, Low Cost Genotype Data In Soybean [Glycine Max (L.) Merr.], Mary M. Happ, Haichuan Wang, George L. Graef, David L. Hyten

Agronomy & Horticulture -- Faculty Publications

Obtaining genome-wide genotype information for millions of SNPs in soybean [Glycine max (L.) Merr.] often involves completely resequencing a line at 5X or greater coverage. Currently, hundreds of soybean lines have been resequenced at high depth levels with their data deposited in the NCBI Short Read Archive. This publicly available dataset may be leveraged as an imputation reference panel in combination with skim (low coverage) sequencing of new soybean genotypes to economically obtain high-density SNP information. Ninety-nine soybean lines resequenced at an average of 17.1X were used to generate a reference panel, with over 10 million SNPs called using ...


Impact Of Hurricane Katrina On The Coastal Systems Of Southern Louisiana, Wen-Ching Chuang, Tarsha Eason, Ahjond Garmestani, Caleb Roberts Jan 2019

Impact Of Hurricane Katrina On The Coastal Systems Of Southern Louisiana, Wen-Ching Chuang, Tarsha Eason, Ahjond Garmestani, Caleb Roberts

Agronomy & Horticulture -- Faculty Publications

Natural disasters, such as hurricanes and forest fires, could trigger collapse and reorganization of social-ecological systems. In the face of external perturbations, a resilient system would have capacity to absorb impacts, adapt to change, learn, and if needed, reorganize within the same regime. Within this context, we asked how human and natural systems in Louisiana responded to Hurricane Katrina, and how the natural disaster altered the status of these systems. This paper discusses community resilience to natural hazards and addresses the limitations for assessing disaster resilience. Furthermore, we assessed social and environmental change in New Orleans and southern Louisiana through ...


Genome-Wide Association Study For Multiple Biotic Stress Resistance In Synthetic Hexaploid Wheat, Madhav Bhatta, Alexey Morgounov, Vikas Belamkar, Stephen N. Wegulo, Abdelfattah A. Dababat, Gul Erginbas-Orakci, Mustapha El Bouhssini, Pravin Gautam, Jesse Poland, Nilufer Akci, Lutfu Demir, Ruth Wanyera, P. Stephen Baenziger Jan 2019

Genome-Wide Association Study For Multiple Biotic Stress Resistance In Synthetic Hexaploid Wheat, Madhav Bhatta, Alexey Morgounov, Vikas Belamkar, Stephen N. Wegulo, Abdelfattah A. Dababat, Gul Erginbas-Orakci, Mustapha El Bouhssini, Pravin Gautam, Jesse Poland, Nilufer Akci, Lutfu Demir, Ruth Wanyera, P. Stephen Baenziger

Agronomy & Horticulture -- Faculty Publications

Genetic resistance against biotic stress is a major goal in many wheat breeding programs. However, modern wheat cultivars have a limited genetic variation for disease and pest resistance and there is always a possibility of the evolution of new diseases and pests to overcome previously identified resistance genes. A total of 125 synthetic hexaploid wheats (SHWs; 2n = 6x = 42, AABBDD, Triticum aestivum L.) were characterized for resistance to fungal pathogens that cause wheat rusts (leaf; Puccinia triticina, stem; P. graminis f.sp. tritici, and stripe; P. striiformis f.sp. tritici) and crown rot (Fusarium spp.); cereal cyst nematode ...


Utilizing Trait Networks And Structural Equation Models As Tools To Interpret Multi‑Trait Genome‑Wide Association Studies, Mehdi Momen, Malachy T. Campbell, Harkamal Walia, Gota Morota Jan 2019

Utilizing Trait Networks And Structural Equation Models As Tools To Interpret Multi‑Trait Genome‑Wide Association Studies, Mehdi Momen, Malachy T. Campbell, Harkamal Walia, Gota Morota

Agronomy & Horticulture -- Faculty Publications

Background: Plant breeders seek to develop cultivars with maximal agronomic value, which is often assessed using numerous, often genetically correlated traits. As intervention on one trait will affect the value of another, breeding decisions should consider the relationships among traits in the context of putative causal structures (i.e., trait networks). While multi-trait genome-wide association studies (MTM-GWAS) can infer putative genetic signals at the multivariate scale, standard MTM-GWAS does not accommodate the network structure of phenotypes, and therefore does not address how the traits are interrelated. We extended the scope of MTM-GWAS by incorporating trait network structures into GWAS using ...


Identification Of Quantitative Trait Loci For Plant Height, Crown Diameter, And Plant Biomass In A Pseudo-F2 Population Of Switchgrass, Megan Taylor, Carl-Erik Tornqvist, Xiongwei Zhao, R. W. Doerge, Michael D. Casler, Yiwei Jiang Jan 2019

Identification Of Quantitative Trait Loci For Plant Height, Crown Diameter, And Plant Biomass In A Pseudo-F2 Population Of Switchgrass, Megan Taylor, Carl-Erik Tornqvist, Xiongwei Zhao, R. W. Doerge, Michael D. Casler, Yiwei Jiang

Agronomy & Horticulture -- Faculty Publications

Switchgrass (Panicum virgatum) is a perennial warm-season grass that produces high biomass yield. Identification of mechanisms for genetic regulation of biomass traits has potential to facilitate genetic manipulation of switchgrass for enhancing biomass yield. The objective of this study was to identify quantitative trait loci for biomass-related traits in a pseudo-F2 population of switchgrass derived from an upland cross with a lowland switchgrass cultivar. Plant height (HT), crown diameter (CD), and plant biomass (PB) were assessed in field studies in 2015 and 2016. Plant height was positively correlated with PB in both years but only correlated with CD in ...


Selection Of Bread Wheat For Low Grain Cadmium Concentration At The Seedling Stage Using Hydroponics Versus Molecular Markers, Caixia Liu, Mary J. Guttieri, Brian M. Waters, Kent M. Eskridge, P. Stephen Baenziger Jan 2019

Selection Of Bread Wheat For Low Grain Cadmium Concentration At The Seedling Stage Using Hydroponics Versus Molecular Markers, Caixia Liu, Mary J. Guttieri, Brian M. Waters, Kent M. Eskridge, P. Stephen Baenziger

Agronomy & Horticulture -- Faculty Publications

The excessive accumulation of Cd in harvested crops grown on high-Cd soils has increased public concerns for food safety. Due to the high consumption of bread wheat (Triticum aestivum L.) per capita, high concentrations of Cd in wheat grain can significantly affect human health. Breeding is a promising way to reduce grain Cd concentration. However, a lack of efficient selection methods impedes breeding for low grain Cd concentration in bread wheat. In this study, a recombinant inbred population segregating for grain Cd concentration was used to assess the efficacy of two selection methods for decreasing grain Cd concentration in bread ...


Joint Use Of Genome, Pedigree, And Their Interaction With Environment For Predicting The Performance Of Wheat Lines In New Environments, Réka Howard, Daniel Gianola, Osval Montesinos-Lopez, Philomin Juliana, Ravi Singh, Jesse Poland, Sandesh Shrestha, Paulino Pérez-Rodriguez, José Crossa, Diego Jarquin Jan 2019

Joint Use Of Genome, Pedigree, And Their Interaction With Environment For Predicting The Performance Of Wheat Lines In New Environments, Réka Howard, Daniel Gianola, Osval Montesinos-Lopez, Philomin Juliana, Ravi Singh, Jesse Poland, Sandesh Shrestha, Paulino Pérez-Rodriguez, José Crossa, Diego Jarquin

Agronomy & Horticulture -- Faculty Publications

Genome-enabled prediction plays an essential role in wheat breeding because it has the potential to increase the rate of genetic gain relative to traditional phenotypic and pedigree-based selection. Since the performance of wheat lines is highly influenced by environmental stimuli, it is important to accurately model the environment and its interaction with genetic factors in prediction models. Arguably, multi-environmental best linear unbiased prediction (BLUP) may deliver better prediction performance than single-environment genomic BLUP. We evaluated pedigree and genome-based prediction using 35,403 wheat lines from the Global Wheat Breeding Program of the International Maize and Wheat Improvement Center (CIMMYT). We ...