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Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

2019

University of Massachusetts Medical School

Cellular and Molecular Physiology

Radiology Publications and Presentations

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

The Central Role Of The Tail In Switching Off 10s Myosin Ii Activity, Shixin Yang, Kyounghwan Lee, John L. Woodhead, Osamu Sato, Mitsuo Ikebe, Roger Craig Sep 2019

The Central Role Of The Tail In Switching Off 10s Myosin Ii Activity, Shixin Yang, Kyounghwan Lee, John L. Woodhead, Osamu Sato, Mitsuo Ikebe, Roger Craig

Radiology Publications and Presentations

Myosin II is a motor protein with two heads and an extended tail that plays an essential role in cell motility. Its active form is a polymer (myosin filament) that pulls on actin to generate motion. Its inactive form is a monomer with a compact structure (10S sedimentation coefficient), in which the tail is folded and the two heads interact with each other, inhibiting activity. This conformation is thought to function in cells as an energy-conserving form of the molecule suitable for storage as well as transport to sites of filament assembly. The mechanism of inhibition of the compact molecule ...


Proteome Of The Central Apparatus Of A Ciliary Axoneme, Lei Zhao, Yuqing Hou, Tyler Picariello, Branch Craige, George B. Witman Jun 2019

Proteome Of The Central Apparatus Of A Ciliary Axoneme, Lei Zhao, Yuqing Hou, Tyler Picariello, Branch Craige, George B. Witman

Radiology Publications and Presentations

Nearly all motile cilia have a "9+2" axoneme containing a central apparatus (CA), consisting of two central microtubules with projections, that is essential for motility. To date, only 22 proteins are known to be CA components. To identify new candidate CA proteins, we used mass spectrometry to compare axonemes of wild-type Chlamydomonas and a CA-less mutant. We identified 44 novel candidate CA proteins, of which 13 are conserved in humans. Five of the latter were studied more closely, and all five localized to the CA; therefore, most of the other candidates are likely to also be CA components. Our ...


Getting Into The Thick (And Thin) Of It, Thomas C. Irving, Roger Craig Feb 2019

Getting Into The Thick (And Thin) Of It, Thomas C. Irving, Roger Craig

Radiology Publications and Presentations

The heart is a highly regulated system in which a combination of mechanisms work together to match cardiac output to the needs of the body. Loss of this regulation characterizes many cardiomyopathies, where muscle is either hypo- or hypercontractile. For many years, x-ray diffraction has been used to study the structural basis of myofilament length-dependent activation...