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Full-Text Articles in Life Sciences

Secondary Metabolism Inducing Treatments During In Vitro Development Of Turmeric (Curcuma Longa L.) Rhizomes, Matthew M. Cousins, Jeffrey W. Adelberg, Feng Chen, James Rieck Jul 2014

Secondary Metabolism Inducing Treatments During In Vitro Development Of Turmeric (Curcuma Longa L.) Rhizomes, Matthew M. Cousins, Jeffrey W. Adelberg, Feng Chen, James Rieck

Jeffrey W Adelberg

Turmeric (Curcuma longa L.) plants that were grown in vitro for 17 or 22 weeks as a fed-batch culture in 2.5 L vessels yielded 39 to 43 g and 62 to 70 g of fresh rhizomes per vessel, respectively (95 % confidence interval). The MS liquid medium was maintained at 6 % sucrose through media addition twice during the experiment. Various methods were employed in attempts to increase secondary metabolism. Antioxidant and total phenolics assays were employed to characterize phytochemical activity. A first experiment exposed four clones to phenylalanine and/or methyl jasmonate (MeJa) from week 12 to 17 in culture ...


Short-Term And Long-Term Time Course Studies Of Turmeric (Curcuma Longa L.) Microrhizome Development In Vitro, Jeffrey Adelberg, Matthew M. Cousins Jul 2014

Short-Term And Long-Term Time Course Studies Of Turmeric (Curcuma Longa L.) Microrhizome Development In Vitro, Jeffrey Adelberg, Matthew M. Cousins

Jeffrey W Adelberg

Turmeric (Curcuma longa L.) plantlets were cultured in MS (Murashige and Skoog Physiol Plant 15:473–497, 1962) liquid medium with 6% sucrose. Microrhizome development was observed in the presence of methyl jasmonate (MeJa) (0, 5 and 16 μM) and benzyladenine (BA) (0, 0.32 and 1 μM). Leaf, root, rhizome growth, and sugar use were measured weekly for 6 weeks in small vessels (180 ml) and four times in 23 weeks in larger vessels (2.5 l). MeJa reduced leaf, root, and rhizome biomass. BA had a positive effect on biomass accumulation. Microrhizome mass increased at a linear rate ...


Sucrose, Water And Nutrient Use During State Ii Multiplication Of Two Turmeric Clones (Curcuma Longa L.) In Liquid Medium, Jeffrey Adelberg Jul 2014

Sucrose, Water And Nutrient Use During State Ii Multiplication Of Two Turmeric Clones (Curcuma Longa L.) In Liquid Medium, Jeffrey Adelberg

Jeffrey W Adelberg

Two clones of turmeric (Cucruma longa L.) were assessed nine times during a 28-day culture period in double phosphate MS liquid medium containing 2%, 4% and 6% sucrose. The phase of rapid shoot bud multiplication lasted for 21 days or less, in both clones in all media. The maximum multiplication rate was 3.4× at 20 and 19 days, with 3.9% or 4.7% initial sucrose concentration being optimal for the clones 9-3 and 35-1, respectively. Dry mass increased over the entire culture period and was greatest with 4.8% or at least 6% initial sucrose concentration for the ...


Efficiency In Thin-Film Liquid System For Hosta Micropropagation, Jeffrey Adelberg Jul 2014

Efficiency In Thin-Film Liquid System For Hosta Micropropagation, Jeffrey Adelberg

Jeffrey W Adelberg

Three varieties of Hosta (‘Striptease’, ‘Minuteman’ and ‘Stiletto’) at four densities (40, 80, 120 and 200 explants per litre) were micropropagated on semi-solid agar and a thin-film liquid system with intermittent wetting of plant tissue. The mechanics of wetting by a small wave front required a larger rectangular vessel (11 × 27=297 cm 2) compared to the common cylindrical baby food jar (18 cm 2). Plants multiplied more rapidly in the agitated thin-film system than on agar. Lower plant densities increased rates of multiplication in liquid, but had little or no effect on multiplication rate on agar. Increasing plant density ...


Unitized, Nonselective Cutting Of In Vitro Watermelon, Jeff W. Adelberg, Y Alper, R E. Young, B B. Rhodes Jun 2014

Unitized, Nonselective Cutting Of In Vitro Watermelon, Jeff W. Adelberg, Y Alper, R E. Young, B B. Rhodes

Jeffrey W Adelberg

Unitized, nonselective mass cutting of Stage II Citrullus lanatus cv. Charlee (watermelon) plant tissue cultures has been achieved with various configurations and sizes of wire cutter devices. Time studies revealed that the wire cutters increased the productivity of the cutting function over conventional scalpel and forceps by a factor of 14, Total transfer productivity, including the manual functions of removing tissue from an initial culture vessel and filling (sorting and placing cut tissue segments) new vessels, was increased by a factor of 1,8, The square grid-type cutting devices yielded from 48 to 59% as many viable bud clusters per ...


Approaching Mechanization Of Plant Micropropagation, Jeffrey Adelberg, R E. Young, A Hale, N D. Camper Jun 2014

Approaching Mechanization Of Plant Micropropagation, Jeffrey Adelberg, R E. Young, A Hale, N D. Camper

Jeffrey W Adelberg

Investigations of materials and methods for growing plant tissue in a continual-flow, liquid nutrient medium as an alternative to semisolid agar medium have been made. Enhanced growth of plant tissue on microporouspolypropylene membranes floating on a liquid nutrient has been demonstrated. Moreover, in vitro plantlets on the microporous membrane are free from entanglement with the support matrix and readily available to mechanizedhandling. Trained growth of plantlets through polypropylene netting shows potential for mechanization by mass handling (separation, singulation, and transfer) of plant tissue cultures.


Winter Greenhouse Propagation Of Clematis X Jackmannii With Capillary Surface Material Fibers, Jeffrey Adelberg, Joshua Giordano-Silliman Jun 2014

Winter Greenhouse Propagation Of Clematis X Jackmannii With Capillary Surface Material Fibers, Jeffrey Adelberg, Joshua Giordano-Silliman

Jeffrey W Adelberg

No abstract provided.


Mass Handling Of Watermelon Microcuttings, Jeffrey Adelberg, Y Alper, R E. Young, B B. Rhodes Jun 2014

Mass Handling Of Watermelon Microcuttings, Jeffrey Adelberg, Y Alper, R E. Young, B B. Rhodes

Jeffrey W Adelberg

Modifications were made in the configurations of the unitizing, nonselective wire cutters used by Alper et al.(1992) for mass cuttings of Stage II Citrullus lanatus cv. Charlee (watermelon) plant tissue cultures to further enhance productivity. Mounting the cutter in an inverted position over the receiving vessel eliminated time required for filling. This cut-and-dump technique became 4.8 times more productive for the total transfer process than the conventional scalpel and forceps technique when both time and yield of cut segments with visible buds were considered. A concept for growing fewer, larger tissue clusters per vessel in mini-trays with orienting ...


A New Synthetic Amphidiploid In Cucumis From A C. Sativus X C. Hystrix F1 Interspecific Hybrid, Jeffrey Adelberg, Jinfeng Chen, Jack E. Staub, Halina Skorupska, Billy Rhodes Jun 2014

A New Synthetic Amphidiploid In Cucumis From A C. Sativus X C. Hystrix F1 Interspecific Hybrid, Jeffrey Adelberg, Jinfeng Chen, Jack E. Staub, Halina Skorupska, Billy Rhodes

Jeffrey W Adelberg

CT. A synthetic amphidiploid was developed through organogenesis in tissue culturefrom an interspecific F1hybrid betweenCucumis sativus L. andC. hystrix Chakr. The resultsfrom flow cytometry indicated that, on average, 7.3% of the regenerants with unique morphology were chromosome-doubled F1hybrids. The 2C DNA content of the original F1hybrid was1.17 pg, and the 4C DNA content was 2.35 pg. Frequency of chromosome doubling between thetwo genotypes used in this study varied considerably.


In Vitro Storage Of Hosta Micropropagules - Effect Of Media Sucrose On Post-Storage Recovery, Jeffrey Adelberg, Vani Gollagunta, Nihal C. Rajapakse Jun 2014

In Vitro Storage Of Hosta Micropropagules - Effect Of Media Sucrose On Post-Storage Recovery, Jeffrey Adelberg, Vani Gollagunta, Nihal C. Rajapakse

Jeffrey W Adelberg

No abstract provided.