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Amoeboid Form Of Blastocystis Hominis - A Detailed Ultrastructural Insight, Tan Tian Chye Jan 2006

Amoeboid Form Of Blastocystis Hominis - A Detailed Ultrastructural Insight, Tan Tian Chye

Tan Tian Chye

The amoeboid form of Blastocystis hominis has been reported infrequently, and its morphological descriptions have yielded conflicting and confusing reports. In the present study, we used the amoeboid forms seen predominantly in symptomatic patients infected with Blastocystis to provide detailed descriptions on the fine surface structure and intracellular morphology. Scanning electron microscopy revealed the irregular shape of the amoeboid form, with an intercalated fibrillar structure and a highly convoluted surface with deep indentations and projected pseudopodia. Transmission electron microscopy showed the existence of two types of amoeboid forms of B. hominis in in vitro culture, one with a large central ...


Predominance Of Amoeboid Forms Of Blastocystis Hominis In Isolates From Symptomatic Patients, Tan Tian Chye Jan 2006

Predominance Of Amoeboid Forms Of Blastocystis Hominis In Isolates From Symptomatic Patients, Tan Tian Chye

Tan Tian Chye

Blastocystis hominis is one of the most common human parasites that inhabit the intestinal tract. Conflicting reports continue to exist regarding the existence and the functional role of the amoeboid forms in the life cycle of the parasite. The present study investigates the presence of these forms in 20 isolates obtained from ten symptomatic and asymptomatic patients respectively. A total of 10,000 parasite cells per ml from each isolate were inoculated into three culture tubes each containing 3 ml of Jones' medium supplemented with 10% horse serum, incubated at 37 degrees C. The contents were examined daily for 10 ...


Pcr Fingerprinting Of Blastocystis Isolated From Symptomatic And Asymptomatic Human Hosts, Tan Tian Chye Jan 2006

Pcr Fingerprinting Of Blastocystis Isolated From Symptomatic And Asymptomatic Human Hosts, Tan Tian Chye

Tan Tian Chye

Genomic DNA from 16 Blastocystis hominis isolates comprising of eight asymptomatic isolates (A1-A8) and eight symptomatic isolates (S1-S8) was amplified by arbitrarily primed polymerase chain reaction (AP-PCR) using 38 arbitrary 10-mer primers. Six primers (A10, B5, C20, D1, F6, and F10) generated reproducible DNA fingerprints. AP-PCR amplification revealed similar DNA fingerprints among all symptomatic isolates (S1-S8) with common bands at 850 bp using primer A10, 920 bp using primer B5, and 1.3 kbp using primer D1. Isolates A1, A3, A4, A5, A6, and A7 showed similar DNA banding patterns and all asymptomatic isolates (A1-A8) shared a major band at ...