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Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

2006

Selected Works

Bowling Green State University

Articles 1 - 1 of 1

Full-Text Articles in Life Sciences

Imaging Gene Expression In Live Transgenic Mice After Providing Luciferin In Drinking Water, Daniel J. Hiler, Megan L. Greenwald, Michael Eric Geusz Aug 2006

Imaging Gene Expression In Live Transgenic Mice After Providing Luciferin In Drinking Water, Daniel J. Hiler, Megan L. Greenwald, Michael Eric Geusz

Michael Eric Geusz

Mice expressing the firefly luciferase gene luc under the control of various gene promoters are used to image long-term changes in tumor growth, infection, development, and circadian rhythms. This novel approach enables ongoing regulation of gene expression to be visualized through repeated imaging of luciferase bioluminescence. Typically, luciferin, the luciferase substrate, is injected into mice before they are anaesthetized for imaging. To avoid the effects of handling and stress from injection on expression of the transgene, oral luciferin delivery methods were tested as an alternative to current methods. For unobscured imaging, a transgenic mouse line containing luc controlled by the ...