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2006

Selected Works

Medicine and Health Sciences

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Articles 1 - 30 of 74

Full-Text Articles in Life Sciences

Aflatoxicosis In Dogs And Dealing With Suspected Contaminated Commercial Foods, Katherine Stenske, J Smith, Shelley Newman, L Newman, Claudia Kirk Jan 2015

Aflatoxicosis In Dogs And Dealing With Suspected Contaminated Commercial Foods, Katherine Stenske, J Smith, Shelley Newman, L Newman, Claudia Kirk

Shelley Newman

No abstract provided.


Calpain Inhibitor Mdl28170 Modulates Abeta Formation By Inhibiting The Formation Of Intermediate Abeta46 And Protecting Abeta From Degradation, Y Dong, J Tan, Mei-Zhen Cui, G Zhao, G Mao, N Singh, Xuemin Xu Jun 2013

Calpain Inhibitor Mdl28170 Modulates Abeta Formation By Inhibiting The Formation Of Intermediate Abeta46 And Protecting Abeta From Degradation, Y Dong, J Tan, Mei-Zhen Cui, G Zhao, G Mao, N Singh, Xuemin Xu

Mei-Zhen Cui

The observations that three major cleavages within the transmembrane domain of APP, namely, the gamma-cleavage, -cleavage, and the newly identified zeta-cleavage, are involved in the generation of secreted Abeta40 and Abeta42 prompted us to determine how the calpain inhibitor III MDL 28170 influences these three cleavages and Abeta formation. With the use of a cell culture system, our data demonstrate that 1) at either high concentrations, or at a low range of concentrations, at early time points, MDL 28170 inhibits the formation of secreted Abeta40 and Abeta42. However, this effect is due to inhibition of the intermediate Abeta46 generation by ...


Analysis Of Genetic Mutations In The 7a7b Open Reading Frame Of Coronavirus Of Cheetahs (Acinonyx Jubatus), Melissa Kennedy, E Moore, R Wilkes, S Citino, Stephen Kania Jan 2012

Analysis Of Genetic Mutations In The 7a7b Open Reading Frame Of Coronavirus Of Cheetahs (Acinonyx Jubatus), Melissa Kennedy, E Moore, R Wilkes, S Citino, Stephen Kania

Melissa A. Kennedy

OBJECTIVE: To analyze the 7a7b genes of the feline coronavirus (FCoV) of cheetahs, which are believed to play a role in virulence of this virus. SAMPLE POPULATION: Biologic samples collected during a 4-year period from 5 cheetahs at the same institution and at 1 time point from 4 cheetahs at different institutions. PROCEDURES: Samples were first screened for FCoV via a reverse transcription-PCR procedure involving primers that encompassed the 3'-untranslated region. Samples that yielded positive assay results were analyzed by use of primers that targeted the 7a7b open reading frames. The nucleotide sequences of the 7a7b amplification products were ...


Pulmonary Fibroblasts Stimulate The Proliferation Of Cell Lines From Human Lung Adenocarcinomas, Maria Cekanova, T Masi, Howard Plummer, M Majidi, P Fedorocko, Hildegard Schuller Aug 2011

Pulmonary Fibroblasts Stimulate The Proliferation Of Cell Lines From Human Lung Adenocarcinomas, Maria Cekanova, T Masi, Howard Plummer, M Majidi, P Fedorocko, Hildegard Schuller

Howard K. Plummer III

Human lung cancer cell lines are widely used to test anticancer drugs. These in-vitro tests, however, preclude the detection of responses to paracrine factors from surrounding stroma. We have cocultured pulmonary fibroblasts CCD-19Lu, from a healthy donor, or HLF-A, from a patient with epidermoid carcinoma of the lung, with two human pulmonary adenocarcinoma cell lines to test the hypothesis that the fibroblasts stimulate the growth of the tumor cells. Both fibroblast cell lines significantly increased the proliferation of the pulmonary adenocarcinoma cell lines in 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide assays, with HLF-A fibroblasts yielding the most pronounced responses ...


Evaluation Of Canine Serum For The Presence Of Anti-Retinal Antibodies In Sudden Acquired Retinal Dengeneration Syndrome, R Keller, Stephen Kania, Diane Hendrix, Daniel Ward, K Abrams May 2011

Evaluation Of Canine Serum For The Presence Of Anti-Retinal Antibodies In Sudden Acquired Retinal Dengeneration Syndrome, R Keller, Stephen Kania, Diane Hendrix, Daniel Ward, K Abrams

Diane Hendrix

Objective: To determine the presence of serum antiretinal antibodies in sudden acquired retinal degeneration syndrome (SARDS) affected dogs and the size of the antigen to which these antibodies bind via the use of enzyme-linked immunosorbent assay (ELISA) and Western blot immunoassays. Animals studied: Serum was collected from 13 dogs affected by SARDS and five dogs with normal ocular examinations. Procedures: All serum samples were subjected to ELISA with saline-soluble canine retinal tissue and Western blot analyses with SDS solubilized normal canine retinal tissue as the antigen. Antirecoverin (23 kDa) and antiheat shock cognate (65 kDa) antibodies were used as positive ...


Evaluation Of Canine Serum For The Presence Of Anti-Retinal Antibodies In Sudden Acquired Retinal Dengeneration Syndrome, R Keller, Stephen Kania, Diane Hendrix, Daniel Ward, K Abrams Feb 2011

Evaluation Of Canine Serum For The Presence Of Anti-Retinal Antibodies In Sudden Acquired Retinal Dengeneration Syndrome, R Keller, Stephen Kania, Diane Hendrix, Daniel Ward, K Abrams

Daniel A. Ward

Objective: To determine the presence of serum antiretinal antibodies in sudden acquired retinal degeneration syndrome (SARDS) affected dogs and the size of the antigen to which these antibodies bind via the use of enzyme-linked immunosorbent assay (ELISA) and Western blot immunoassays. Animals studied: Serum was collected from 13 dogs affected by SARDS and five dogs with normal ocular examinations. Procedures: All serum samples were subjected to ELISA with saline-soluble canine retinal tissue and Western blot analyses with SDS solubilized normal canine retinal tissue as the antigen. Antirecoverin (23 kDa) and antiheat shock cognate (65 kDa) antibodies were used as positive ...


The Plant Structure Ontology, A Unified Vocabulary Of Anatomy And Morphology Of A Flowering Plant, Katica Ilic, Elizabeth A. Kellogg, Pankaj Jaiswal, Felipe Zapata, Peter F. Stevens, Leszek P. Vincent, Shulamit Avraham, Leonore Reiser, Anuradha Pujar, Martin M. Sachs, Noah T. Whitman, Susan R. Mccouch, Mary L. Schaeffer, Doreen H. Ware, Lincoln D. Stein, Seung Y. Rhee Dec 2006

The Plant Structure Ontology, A Unified Vocabulary Of Anatomy And Morphology Of A Flowering Plant, Katica Ilic, Elizabeth A. Kellogg, Pankaj Jaiswal, Felipe Zapata, Peter F. Stevens, Leszek P. Vincent, Shulamit Avraham, Leonore Reiser, Anuradha Pujar, Martin M. Sachs, Noah T. Whitman, Susan R. Mccouch, Mary L. Schaeffer, Doreen H. Ware, Lincoln D. Stein, Seung Y. Rhee

Peter Stevens

Formal description of plant phenotypes and standardized annotation of gene expression and protein localization data require uniform terminology that accurately describes plant anatomy and morphology. This facilitates cross species comparative studies and quantitative comparison of phenotypes and expression patterns. A major drawback is variable terminology that is used to describe plant anatomy and morphology in publications and genomic databases for different species. The same terms are sometimes applied to different plant structures in different taxonomic groups. Conversely, similar structures are named by their species-specific terms. To address this problem, we created the Plant Structure Ontology (PSO), the first generic ontological ...


Wavelet-Based Functional Mixed Models To Characterize Population Heterogeneity In Accelerometer Profiles: A Case Study. , Jeffrey S. Morris, Cassandra Arroyo, Brent A. Coull, Louise M. Ryan, Steven L. Gortmaker Dec 2006

Wavelet-Based Functional Mixed Models To Characterize Population Heterogeneity In Accelerometer Profiles: A Case Study. , Jeffrey S. Morris, Cassandra Arroyo, Brent A. Coull, Louise M. Ryan, Steven L. Gortmaker

Jeffrey S. Morris

We present a case study illustrating the challenges of analyzing accelerometer data taken from a sample of children participating in an intervention study designed to increase physical activity. An accelerometer is a small device worn on the hip that records the minute-by-minute activity levels of the child throughout the day for each day it is worn. The resulting data are irregular functions characterized by many peaks representing short bursts of intense activity. We model these data using the wavelet-based functional mixed model. This approach incorporates multiple fixed effects and random effect functions of arbitrary form, the estimates of which are ...


Alternative Probeset Definitions For Combining Microarray Data Across Studies Using Different Versions Of Affymetrix Oligonucleotide Arrays, Jeffrey S. Morris, Chunlei Wu, Kevin R. Coombes, Keith A. Baggerly, Jing Wang, Li Zhang Dec 2006

Alternative Probeset Definitions For Combining Microarray Data Across Studies Using Different Versions Of Affymetrix Oligonucleotide Arrays, Jeffrey S. Morris, Chunlei Wu, Kevin R. Coombes, Keith A. Baggerly, Jing Wang, Li Zhang

Jeffrey S. Morris

Many published microarray studies have small to moderate sample sizes, and thus have low statistical power to detect significant relationships between gene expression levels and outcomes of interest. By pooling data across multiple studies, however, we can gain power, enabling us to detect new relationships. This type of pooling is complicated by the fact that gene expression measurements from different microarray platforms are not directly comparable. In this chapter, we discuss two methods for combining information across different versions of Affymetrix oligonucleotide arrays. Each involves a new approach for combining probes on the array into probesets. The first approach involves ...


Organochlorine Levels In Seawater And Sediment From The Florida Gulf Coast, If Present, Are Low. , Richard B. Philp Nov 2006

Organochlorine Levels In Seawater And Sediment From The Florida Gulf Coast, If Present, Are Low. , Richard B. Philp

Richard B. Philp

Seawater and sediment from the Florida panhandle coast were assayed for a panel of 28 organochlorine insecticides and PCBs. No samples exeeded the detection limits of the analytical technique, which were well below levels reported for other ocean locales. Organochlorine levels in this area of the Gulf must be very low. This does not preclude their biomagnificatioon up the food web.


Prepms: Tof Ms Data Graphical Preprocessing Tool, Yuliya V. Karpievitch, Elizabeth G. Hill, Adam J. Smolka, Jeffrey S. Morris, Kevin R. Coombes, Keith A. Baggerly, Jonas S. Almeida Nov 2006

Prepms: Tof Ms Data Graphical Preprocessing Tool, Yuliya V. Karpievitch, Elizabeth G. Hill, Adam J. Smolka, Jeffrey S. Morris, Kevin R. Coombes, Keith A. Baggerly, Jonas S. Almeida

Jeffrey S. Morris

We introduce a simple-to-use graphical tool that enables researchers to easily prepare time-of-flight mass spectrometry data for analysis. For ease of use, the graphical executable provides default parameter settings experimentally determined to work well in most situations. These values can be changed by the user if desired. PrepMS is a stand-alone application made freely available (open source), and is under the General Public License (GPL). Its graphical user interface, default parameter settings, and display plots allow PrepMS to be used effectively for data preprocessing, peak detection, and visual data quality assessment.


Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough Oct 2006

Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough

Eric Blough

This study tested the hypothesis that age-related changes in the dystrophin–glycoprotein complex (DGC) may precede age-associated alterations in muscle morphology and function. Compared to those in adult (6 month) rats, extensor digitorum longus (EDL) and soleus muscle mass was decreased in old (30 month) and very old (36 month) Fischer 344/NNiaHSD 3 Brown Norway/ BiNia rats. The amount of dystrophin, b-dystroglycan, and a-sarcoglycan increased with aging in the EDL and decreased with aging in the soleus. a-Dystroglycan levels were increased with aging in both muscles and displayed evidence of altered glycosylation. Immunostaining for the presence of antibody infiltration ...


Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough Oct 2006

Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough

Kevin M Rice

This study tested the hypothesis that age-related changes in the dystrophin–glycoprotein complex (DGC) may precede age-associated alterations in muscle morphology and function. Compared to those in adult (6 month) rats, extensor digitorum longus (EDL) and soleus muscle mass was decreased in old (30 month) and very old (36 month) Fischer 344/NNiaHSD 3 Brown Norway/ BiNia rats. The amount of dystrophin, b-dystroglycan, and a-sarcoglycan increased with aging in the EDL and decreased with aging in the soleus. a-Dystroglycan levels were increased with aging in both muscles and displayed evidence of altered glycosylation. Immunostaining for the presence of antibody infiltration ...


Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough Oct 2006

Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough

Deborah L Preston

This study tested the hypothesis that age-related changes in the dystrophin–glycoprotein complex (DGC) may precede age-associated alterations in muscle morphology and function. Compared to those in adult (6 month) rats, extensor digitorum longus (EDL) and soleus muscle mass was decreased in old (30 month) and very old (36 month) Fischer 344/NNiaHSD 3 Brown Norway/ BiNia rats. The amount of dystrophin, b-dystroglycan, and a-sarcoglycan increased with aging in the EDL and decreased with aging in the soleus. a-Dystroglycan levels were increased with aging in both muscles and displayed evidence of altered glycosylation. Immunostaining for the presence of antibody infiltration ...


Nnk Activates Erk1/2 And Creb/Atf-1 Via Beta-1-Ar And Egfr Signaling In Human Lung Adenocarcinoma And Small Airway Epithelial Cells, E Laag, M Majidi, Maria Cekanova, T Masi, T Takahashi, Hildegard Schuller Sep 2006

Nnk Activates Erk1/2 And Creb/Atf-1 Via Beta-1-Ar And Egfr Signaling In Human Lung Adenocarcinoma And Small Airway Epithelial Cells, E Laag, M Majidi, Maria Cekanova, T Masi, T Takahashi, Hildegard Schuller

Maria Cekanova MS, RNDr, PhD

We have shown that the tobacco nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is an agonist for -adrenergic receptors (beta-ARs) and increased DNA synthesis of human lung adenocarcinoma cells with features of bronchiolar Clara cells by binding to these receptors. Using a cell line derived from a human pulmonary adenocarcinoma with Clara cell phenotype (PACC) and immortalized human small airway epithelial cells (HPLD1), the putative cells of origin of this cancer type, our current studies have analyzed signaling initiated by binding of NNK to the beta 1-AR. NNK upregulated ERK1/2 and CREB/ATF-1 phosphorylation in a PKA-dependent manner in ...


Clinical Evaluation Of Dietary Modification For Treatment Of Spontaneous Chronic Kidney Disease In Cats, S Ross, C Osborne, Claudia Kirk, S Lowry, L Koehler, D Polzin Sep 2006

Clinical Evaluation Of Dietary Modification For Treatment Of Spontaneous Chronic Kidney Disease In Cats, S Ross, C Osborne, Claudia Kirk, S Lowry, L Koehler, D Polzin

Claudia A Kirk

Objective-To determine whether a renal diet modified in protein, phosphorus, sodium, and lipid content was superior to an adult maintenance diet in minimizing uremic episodes and mortality rate in cats with stage 2 or 3 chronic kidney disease (CKD). Design-Double-masked, randomized, controlled clinical trial. Animals-45 client-owned cats with spontaneous stage 2 or 3 CKD. Procedures-Cats were randomly assigned to an adult maintenance diet (n = 23 cats) or a renal diet (22) and evaluated trimonthly for up to 24 months. Efficacy of the renal diet, compared with the maintenance diet, in minimizing uremia, renal-related deaths, and all causes of death was ...


Acsm's Metabolic Calculations Handbook, Stephen Glass, Greg Dwyer Aug 2006

Acsm's Metabolic Calculations Handbook, Stephen Glass, Greg Dwyer

Stephen C Glass

This handbook provides a step-by-step approach to using metabolic equations, from basic math principles to applying the equations to an exercise plan. Chapters focus separately on each equation, provide an easy-to-follow process of solving, and demonstrate the varied uses of the equation in clinical as well as fitness settings.

Each chapter includes a set of problems that focus on real-world applications of the equation. Step-by-step problem solution explanations are provided at the end of each chapter. A comprehensive exam at the end of the book tests the reader's skill in using the equations.


Neuroprotective And Disease-Modifying Effects Of The Ketogenic Diet, Maciej Gasior, Michael A. Rogawski, Adam L. Hartman Aug 2006

Neuroprotective And Disease-Modifying Effects Of The Ketogenic Diet, Maciej Gasior, Michael A. Rogawski, Adam L. Hartman

Michael A. Rogawski

The ketogenic diet has been in clinical use for over 80 years, primarily for the symptomatic treatment of epilepsy. A recent clinical study has raised the possibility that exposure to the ketogenic diet may confer long-lasting therapeutic benefits for patients with epilepsy. Moreover, there is evidence from uncontrolled clinical trials and studies in animal models that the ketogenic diet can provide symptomatic and disease-modifying activity in a broad range of neurodegenerative disorders including Alzheimer’s disease and Parkinson’s disease, and may also be protective in traumatic brain injury and stroke. These observations are supported by studies in animal models ...


Pulmonary Fibroblasts Stimulate The Proliferation Of Cell Lines From Human Lung Adenocarcinomas, Maria Cekanova, T Masi, Howard Plummer, M Majidi, P Fedorocko, Hildegard Schuller Jul 2006

Pulmonary Fibroblasts Stimulate The Proliferation Of Cell Lines From Human Lung Adenocarcinomas, Maria Cekanova, T Masi, Howard Plummer, M Majidi, P Fedorocko, Hildegard Schuller

Maria Cekanova MS, RNDr, PhD

Human lung cancer cell lines are widely used to test anticancer drugs. These in-vitro tests, however, preclude the detection of responses to paracrine factors from surrounding stroma. We have cocultured pulmonary fibroblasts CCD-19Lu, from a healthy donor, or HLF-A, from a patient with epidermoid carcinoma of the lung, with two human pulmonary adenocarcinoma cell lines to test the hypothesis that the fibroblasts stimulate the growth of the tumor cells. Both fibroblast cell lines significantly increased the proliferation of the pulmonary adenocarcinoma cell lines in 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide assays, with HLF-A fibroblasts yielding the most pronounced responses ...


Avian Vacuolar Myelinopathy Outbreaks At A Southeastern Reservoir, J Fischer, L Lewis-Weis, C Tate, J Gaydos, Richard Gerhold Jr., R Poppenga Jun 2006

Avian Vacuolar Myelinopathy Outbreaks At A Southeastern Reservoir, J Fischer, L Lewis-Weis, C Tate, J Gaydos, Richard Gerhold Jr., R Poppenga

Richard W. Gerhold Jr., DVM, MS, PhD

Avian vacuolar myelinopathy (AVM) is a neurologic disease of unknown etiology that affects bald eagles (Haliaeetus leucocephalus), American coots (Fulica americana), and several species of waterfowl. An unidentified neurotoxin is suspected as the cause of AVM, which has been documented at several reservoirs in the southeastern United States. We conducted diagnostic and epidemiologic studies annually during October-March from 1998-2004 at Clarks Hill/Strom Thurmond Lake on the Georgia/South Carolina border to better understand the disease. Avian vacuolar myelinopathy was confirmed or suspected as the cause of morbidity and mortality of 28 bald eagles, 16 Canada geese (Branta canadensis), six ...


Some Statistical Issues In Microarray Gene Expression Data, Matthew S. Mayo, Byron J. Gajewski, Jeffrey S. Morris Jun 2006

Some Statistical Issues In Microarray Gene Expression Data, Matthew S. Mayo, Byron J. Gajewski, Jeffrey S. Morris

Jeffrey S. Morris

In this paper we discuss some of the statistical issues that should be considered when conducting experiments involving microarray gene expression data. We discuss statistical issues related to preprocessing the data as well as the analysis of the data. Analysis of the data is discussed in three contexts: class comparison, class prediction and class discovery. We also review the methods used in two studies that are using microarray gene expression to assess the effect of exposure to radiofrequency (RF) fields on gene expression. Our intent is to provide a guide for radiation researchers when conducting studies involving microarray gene expression ...


Diverse Mechanisms Of Antiepileptic Drugs In The Development Pipeline, Michael A. Rogawski Jun 2006

Diverse Mechanisms Of Antiepileptic Drugs In The Development Pipeline, Michael A. Rogawski

Michael A. Rogawski

There is a remarkable array of new chemical entities in the current antiepileptic drug (AED) development pipeline. In some cases, the compounds were synthesized in an attempt improve upon the activity of marketed AEDs. In other cases, the discovery of antiepileptic potential was largely serendipitous. Entry into the pipeline begins with the demonstration of activity in one or more animal screening models. Results from testing in a panel of such models provide a basis to differentiate agents and may offer clues as to the mechanism. Target activity may then be defined through cell-based studies, often years after the initial identification ...


Detection Of Overexpressed Cox-2 In Precancerous Lesions Of Hamster Pancreas And Lungs By Molecular Imaging: Implications For Early Diagnosis And Prevention, Hildegard Schuller, George Kabalka, G Smith, A Mereddy, M Akula, Maria Cekanova May 2006

Detection Of Overexpressed Cox-2 In Precancerous Lesions Of Hamster Pancreas And Lungs By Molecular Imaging: Implications For Early Diagnosis And Prevention, Hildegard Schuller, George Kabalka, G Smith, A Mereddy, M Akula, Maria Cekanova

Maria Cekanova MS, RNDr, PhD

The enzyme cyclooxygenase-2 (COX-2) is overexpressed in many cancers, cardiovascular disease, neurodegenerative disorders, and arthritis. Selective inhibitors of COX-2 have been developed as therapeutics or preventive agents for these diseases. However, recent reports have revealed a significant increase in cardiovascular mortality in long-term users of the COX-2 inhibitors Vioxx and Celebrex, emphasizing the need for noninvasive tests that allow the identification of individuals whose COX-2 levels are overexpressed prior to assignment to treatment with these drugs. In this study, we have prepared a radioiodinated analogue of the selective COX-2 inhibitor celecoxib, and verified its binding to the COX-2 enzyme in ...


Reticulocyte Indices In A Canine Model Of Nutritional Iron Deficiency, Michael Fry, Claudia Kirk May 2006

Reticulocyte Indices In A Canine Model Of Nutritional Iron Deficiency, Michael Fry, Claudia Kirk

Claudia A Kirk

BACKGROUND: Reticulocyte indices, especially reticulocyte hemoglobin content (CH retic), have shown promise as markers of iron deficiency (ID), but there have been no prospective investigations of reticulocyte indices in experimental models of ID. OBJECTIVES: The objective of this study was to compare reticulocyte indices with conventional hematologic and biochemical indices as markers of ID in dogs. METHODS: Iron deficiency was induced in 7 dogs by feeding an iron-deficient diet, and corrected by restoring dietary iron and by giving iron parenterally. Blood samples were collected at weekly intervals. Results of hematologic and biochemical tests were compared using t-tests and receiver operator ...


Probability Of Real -Time Detection Vs Probability Of Infection For Aerosolized Biowarfare Agents: A Model Study., Alexander G. Sabelnikov, Vladimir Zhukov, C Ruth Kempf May 2006

Probability Of Real -Time Detection Vs Probability Of Infection For Aerosolized Biowarfare Agents: A Model Study., Alexander G. Sabelnikov, Vladimir Zhukov, C Ruth Kempf

Alexander G Sabelnikov

No abstract provided.


Lysophosphatidic Acid Induces Early Growth Response Gene 1 Expression In Vascular Smooth Muscle Cells: Cre And Sre Mediate The Transcription, Mei-Zhen Cui, E Laag, L Sun, M Tan, G Zhao, Xuemin Xu Apr 2006

Lysophosphatidic Acid Induces Early Growth Response Gene 1 Expression In Vascular Smooth Muscle Cells: Cre And Sre Mediate The Transcription, Mei-Zhen Cui, E Laag, L Sun, M Tan, G Zhao, Xuemin Xu

Xuemin Xu

OBJECTIVE: Lysophosphatidic acid (LPA), one component of oxidized low-density lipoprotein, is a potent bioactive phospholipid. Early growth response gene-1 (Egr-1), an important transcription factor, regulates expression of an array of genes involved in vascular diseases. Whether and how LPA regulates the transcriptional machinery of Egr-1 gene is unknown and is addressed in this study. METHOD AND RESULTS: We found that LPA markedly induces Egr-1 mRNA and protein in aortic smooth muscle cells (SMCs). RNA stability and nuclear run-on assays reveal that LPA-induced Egr-1 gene expression is controlled at the transcriptional level. Reporter gene analyses have shown that the -141 to ...


Scrubby Boys: Scott Young, His Journalism, His Fiction, And His Contributions To The Literature Of Canadian Sport, Don Morrow Apr 2006

Scrubby Boys: Scott Young, His Journalism, His Fiction, And His Contributions To The Literature Of Canadian Sport, Don Morrow

Donald Morrow

No abstract provided.


Activation In Neural Networks Controlling Ingestive Behaviors: What Does It Mean, And How Do We Map And Measure It?, Alan G. Watts, Arshad M. Khan, Graciela Sanchez-Watts, Dawna Salter, Christina M. Neuner Apr 2006

Activation In Neural Networks Controlling Ingestive Behaviors: What Does It Mean, And How Do We Map And Measure It?, Alan G. Watts, Arshad M. Khan, Graciela Sanchez-Watts, Dawna Salter, Christina M. Neuner

Arshad M. Khan, Ph.D.

No abstract provided.


Homeland Security: Engaging The Frontlines - Symposium Proceedings, George H. Baker, Cheryl J. Elliott Apr 2006

Homeland Security: Engaging The Frontlines - Symposium Proceedings, George H. Baker, Cheryl J. Elliott

George H Baker

The rise of the American homeland security endeavor under the leadership of the new Department of Homeland Security has been heralded by several major national strategy documents. These documents have served to organize efforts at top levels within the government and industry. However, the national strategy guidance is not getting to many organizations and people at the grass-roots level who can make the most difference in preventing attacks, protecting systems, and recovering from catastrophic events, viz. the general citizenry, private infrastructure owners, and local governments. To better understand grass-roots issues and solutions, James Madison University, in cooperation with the Federal ...


Biochemical Characterization Of The Major Sorghum Grain Peroxidase, Mamoudou H. Dicko, Harry Gruppen, Riet Hilhorst, Alphons G. J. Voragen, Willen W. H. Van Berkel Apr 2006

Biochemical Characterization Of The Major Sorghum Grain Peroxidase, Mamoudou H. Dicko, Harry Gruppen, Riet Hilhorst, Alphons G. J. Voragen, Willen W. H. Van Berkel

Pr. Mamoudou H. DICKO, PhD

The major cationic peroxidase in sorghum grain (SPC4) , which is ubiquitously present in all sorghum varieties was purified to apparent homogeneity, and found to be a highly basic protein (pI #1;11). MS analysis showed that SPC4 consists of two glycoforms with molecular masses of 34227 and 35629 Da and it contains a type-b heme. Chemical deglycosylation allowed to estimate sugar contents of 3.0% and 6.7% (w ⁄ w) in glycoform I and II, respectively, and a mass of the apoprotein of 33 246 Da. High performance anion exchange chromatography allowed to determine the carbohydrate constituents of the polysaccharide ...