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Full-Text Articles in Life Sciences

Expression And Function Of Alpha3 And Beta2 Neuronal Nicotinic Acetylcholine Receptor Subunits In Hek-293 Cells, Nathan W. Steinhafel Dec 2006

Expression And Function Of Alpha3 And Beta2 Neuronal Nicotinic Acetylcholine Receptor Subunits In Hek-293 Cells, Nathan W. Steinhafel

Theses and Dissertations

Single-cell real-time quantitative RT-PCR was used to characterize the mRNA expression of rat neuronal nicotinic acetylcholine receptor (nAChR) subunits α3 and β2 in CA1 hippocampus stratum radiatum and stratum oriens interneurons. α3β2 co-expression was detected in 43% of interneurons analyzed. The nAChR subtype α3β2 was transiently expressed in cells derived from the human embryonic kidney cell line 293 at mRNA levels found in the CA1. The functional properties of α3β2 in HEK-293 cells were characterized by whole-cell patch clamping using acetylcholine (ACh) as an agonist. The kinetics of α3β2 channels were further analyzed by altering the level of α3 DNA ...


Technique And Performance Level Comparisons Of Male And Female Hammer Throwers, Suzanne M. Konz Dec 2006

Technique And Performance Level Comparisons Of Male And Female Hammer Throwers, Suzanne M. Konz

Theses and Dissertations

The aim of this study was two-fold: 1) what the hammer throwing technique differences between sexes are and 2) what technique parameters help determine throw distance. The performances of the top 16 male and female throwers at the 2003 World Athletic Final and the top 13 male and female throwers from the 2003 USA Track and Field Nationals were examined. Video was captured using three Canon 60 Hz cameras. The best throws of each athlete were digitized and analyzed using the Peak Motus 8.2 motion analysis system. T-tests revealed that athlete mass, athlete height, velocity at release, timing components ...


Brain Stimulation Reward Is Integrated By A Network Of Electrically-Coupled Gaba Neurons, Matthew Brian Lassen Dec 2006

Brain Stimulation Reward Is Integrated By A Network Of Electrically-Coupled Gaba Neurons, Matthew Brian Lassen

Theses and Dissertations

Although it is well-established that animals will self-stimulate electric current to various diverse brain structures, the neural substrate of brain stimulation reward (BSR) has eluded identification since its discovery more than a half-century ago. We show that GABA neurons in the midbrain, hypothalamus and thalamus express connexin-36 (Cx36) gap junctions and couple electrically with dopamine application or by stimulation of the internal capsule (IC), which also supports self-stimulation. The threshold for responding for self-stimulation of the IC is the threshold for coupling between these GABA neurons, the degree of responding for IC ICSS is proportional to the magnitude of electrical ...


Evolution In Neotropical Herpetofauna: Species Boundaries In High Andean Frogs And Evolutionary Genetics In The Lava Lizard Genus Microlophus (Squamata: Tropiduridae): A History Of Colonization And Dispersal, Edgar Benavides Dec 2006

Evolution In Neotropical Herpetofauna: Species Boundaries In High Andean Frogs And Evolutionary Genetics In The Lava Lizard Genus Microlophus (Squamata: Tropiduridae): A History Of Colonization And Dispersal, Edgar Benavides

Theses and Dissertations

In this collection of papers I have summarized my investigations into the field of evolutionary genetics and more specifically into patterns of biodiversity and evolutionary processes. The lizards (and frogs) studied here share common features in that they are largely present in unique environments, which are also regions that are biologically understudied. Most of these taxa show high degrees of endemism, interesting natural history characteristics, and each group manifests distinctive adaptations of general evolutionary interest. My work in the genus Telmatobius has been a progressive approach that began in my MS program, and it first focused on alpha taxonomy, morphological ...


Forage Adaptability Trials For Forage And Seed Production In Bolivia; Effect Of 5 Herbicides On 7 Native Utah Forbs, Joshua C. Voss Dec 2006

Forage Adaptability Trials For Forage And Seed Production In Bolivia; Effect Of 5 Herbicides On 7 Native Utah Forbs, Joshua C. Voss

Theses and Dissertations

The harsh environmental and poor economic conditions of the Bolivian Altiplano require intervention to assist many of those that live there to become economically self-sufficient. We attempted to find introduced dry season reserve forage grasses that could produce enough biomass to be useful as feed for livestock, and that could also produce enough seed to distribute to farmers. While some of the grasses produced reasonable amounts of biomass, none produced seed in quantities that would be even close to being economically viable. The most likely cause of this is that the timing of resources that the grasses need to flower ...


The Role Of Wnt Signaling In Development Of The Ophthalmic Trigeminal Placode., Rhonda Nicole Lassiter Dec 2006

The Role Of Wnt Signaling In Development Of The Ophthalmic Trigeminal Placode., Rhonda Nicole Lassiter

Theses and Dissertations

Cranial placodes are ectodermal regions that contribute extensively to the vertebrate peripheral nervous system. The development of the ophthalmic trigeminal (opV) placode, which gives rise only to sensory neurons of the ophthalmic lobe of the trigeminal ganglion, is a well-studied model of sensory neuron development. While key differentiation processes have been characterized at the tissue and cellular levels, the molecules governing opV placode development have not been well described. This study identifies the canonical Wnt signaling pathway as a regulator of opV trigeminal placode development. Introducing dominant-negative TCF and dominant-active β-catenin expression constructs by in ovo electroporation, we have manipulated ...


Effects Of Telephone Weight Loss Coaching On Body Composition In Adults: A Randomized Controlled Study, Amy Jensen Cook Dec 2006

Effects Of Telephone Weight Loss Coaching On Body Composition In Adults: A Randomized Controlled Study, Amy Jensen Cook

Theses and Dissertations

Objective: To determine the extent to which coaching over the telephone is an effective method in promoting the loss of body weight and body fat percentage. Research Methods and Procedures: Over a period of four months, in a randomized, double blind, placebo-controlled study, 120 overweight and obese (BMI 25-35 kg/m²) adults either received telephone coaching or no coaching. In addition, each participant was randomly assigned to take a supplement or placebo daily. Body weight and body fat percentage were measured at baseline, two months, and four months. Body weight was measured on an electronic scale and body fat percentage ...


Development Of Polymer Monoliths For The Analysis Of Peptides And Proteins, Binghe Gu Dec 2006

Development Of Polymer Monoliths For The Analysis Of Peptides And Proteins, Binghe Gu

Theses and Dissertations

Several novel polymer monoliths for the analysis of peptides and proteins were synthesized using polyethylene glycol diacrylate (PEGDA) as crosslinker. Photo-initiated copolymerization of polyethylene glycol methyl ether acrylate and PEGDA yielded an inert monolith that could be used for size exclusion liquid chromatography of peptides and proteins. This macroscopically uniform monolith did not shrink or swell in either water or tetrahydrofuran. More importantly, it was found to resist adsorption of both acidic and basic proteins in aqueous buffer without any organic solvent additives. A strong cation-exchange polymer monolith was synthesized by copolymerization of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) and PEGDA. A ternary ...


Synthesis And Optical Properties Of Four Oligothiophene-Ruthenium Complexes And Synthesis Of A Bidentate Ligand For C-F Bond Activation, Joseph S. Bair Dec 2006

Synthesis And Optical Properties Of Four Oligothiophene-Ruthenium Complexes And Synthesis Of A Bidentate Ligand For C-F Bond Activation, Joseph S. Bair

Theses and Dissertations

Photovoltaic cells and fluorescence sensing are two important areas of research in chemistry. The combination of photon-activated electron donors with electron acceptors provides a strong platform for the study of optical devices. A series of four oligothiophene-ruthenium complexes has been synthesized. Variation in oligothiophene length and bipyridine substitution allowed comparison of these variables on electronic properties. The longer oligothiophenes display lower energy absorption and emission compared to the shorter ones. Aromatic conjugation appears more complete with para-, rather than meta-, substitution. Oligothiophenes and Ru(bpy)32+ are highly fluorescent individually, but fluorescence is quenched when connected. Bonds of carbon to ...


Phylogenetics Of North American Psoraleeae (Leguminosae): Rates And Dates In A Recent, Rapid Radiation, Ashley N. Egan Dec 2006

Phylogenetics Of North American Psoraleeae (Leguminosae): Rates And Dates In A Recent, Rapid Radiation, Ashley N. Egan

Theses and Dissertations

The scientific discipline of phylogenetics involves understanding the diversity of life in the context of history. Technological advances in molecular biology and computational power have created an exponential increase in the number and kind of phylogenetic studies, allowing exploration of all manner of questions concerning the evolutionary history of a group. This dissertation research is focused on phylogenetic theory and applying these methods to the plant tribe Psoraleeae (Leguminosae; Fabaceae) with an emphasis on North American (NAm) members. First, theory of phylogeny estimation is explored in a review chapter focusing on strengths and weaknesses of phylogenetic methodology. Second, methods are ...


Development Of Methods For The Study Of Phosphoproteins, Zhaoyuan Chen Dec 2006

Development Of Methods For The Study Of Phosphoproteins, Zhaoyuan Chen

Theses and Dissertations

Characterization of phosphoproteins-including detection, identification of phosphoproteins and identification of phosphorylation sites-is mostly done with radiolabeling and proteomic techniques. Three main topics related to phosphoprotein characterization are included in this dissertation. First, large-scale characterization of the CHO (Chinese hamster ovary) cell phosphoproteome was done using two dimensional gel electrophoresis (2DE) separation, visualization of phosphoproteins by radiolabeling or a phosphoprotein specific dye, followed by MALDI-TOF identification. Because radiolabeling of phosphoproteins is very sensitive and straightforward to quantify, such analysis can give a clear picture of the relative phosphosphorylation of proteins present in a sample. But there are also limitations to ...


The Influence Of Membrane Lipid Order On Cell Shape And Microvesiculation In Human Erythrocytes, Laurie Jackson Gonzalez Nov 2006

The Influence Of Membrane Lipid Order On Cell Shape And Microvesiculation In Human Erythrocytes, Laurie Jackson Gonzalez

Theses and Dissertations

Exposure of human erythrocytes to elevated intracellular calcium causes alterations in cell shape and stimulates shedding of the cell membrane in the form of microvesicles. We hypothesized that both the shape transition and microvesiculation are influenced by microscopic membrane physical properties such as lipid order. To test this hypothesis, membrane properties were manipulated by varying the experimental temperature, membrane cholesterol content, and the internal ionic environment. Changes in membrane order were assessed using steady-state fluorescence spectroscopy with an environment-sensitive probe, laurdan. Our observations led us to the following conclusions: 1) the modest temperature dependence of membrane structure observed with laurdan ...


Fgf4 And Wnt5a/Pcp Signaling Promote Limb Outgrowth By Polarizing Limb Mesenchyme, Keri Lynn Low Nov 2006

Fgf4 And Wnt5a/Pcp Signaling Promote Limb Outgrowth By Polarizing Limb Mesenchyme, Keri Lynn Low

Theses and Dissertations

The focus of this study was to elucidate the molecular and cellular mechanisms whereby fibroblast growth factors (FGFs) mediate outgrowth of the limb. Specifically, we examined the epistatic relationship between FGF and Wnt/Planar cell polarity (PCP) signaling in establishing cell polarity as a mechanism for outgrowth. By implanting beads into embryonic limbs and lateral plate mesoderm, we established that FGF activates Wnt5a in a gradient fashion. Once it was established that Wnt5a was expressed at the right time and place to turn on PCP signaling, we investigated the ability of Wnt5a to influence cell migration and/or cell polarity ...


Effects Of Cheatgrass Control On Wyoming Big Sagebrush In Southeastern Utah, Daniel Blaine Eddington Nov 2006

Effects Of Cheatgrass Control On Wyoming Big Sagebrush In Southeastern Utah, Daniel Blaine Eddington

Theses and Dissertations

Critical mule deer (Odocoileus hemionus) winter ranges in southeastern Utah dominated by Wyoming big sagebrush (Artemisia tridentata var. wyomingensis [Beetle and A. Young] Welsh) have developed dense cheatgrass (Bromus tectorum L.) understories. These communities are currently characterized by predominately mature to decadent stands of sagebrush with few perennial grasses and forbs. Sagebrush seedlings and perennial grasses compete for limited resources with annual grasses and forbs. To determine the affects of cheatgrass control on sagebrush growth and reproductive characteristics, imazapic (PLATEAU®, AC 263,222) herbicide was sprayed at 438.5 ml/ha with water and methylated seed oil during active fall ...


Capillary Electrophoresis Of Proteins With Selective On-Line Affinity Monoliths, Jenny Marcela Armenta Blanco Nov 2006

Capillary Electrophoresis Of Proteins With Selective On-Line Affinity Monoliths, Jenny Marcela Armenta Blanco

Theses and Dissertations

The analysis of proteins in biological fluids by capillary electrophoresis (CE) is of interest in clinical chemistry. However, due to low analyte concentrations and poor concentration limits of detection (CLOD), protein analysis by this technique is frequently challenging. Coupling preconcentration techniques with CE greatly improves the CLOD. An on-line preconcentration-CE method that can selectively preconcentrate any protein for which an antibody is available would be very useful for the analysis of low abundance proteins and would establish CE as a major tool in biomarker discovery. To accomplish this, an on-line protein G monolithic preconcentrator CE system for enrichment and separation ...


Biochemical Investigations Of Macular Degeneration: The Significance Of Protein Oxidation Including Novel Methods For Its Study, Sarah Warburton Nov 2006

Biochemical Investigations Of Macular Degeneration: The Significance Of Protein Oxidation Including Novel Methods For Its Study, Sarah Warburton

Theses and Dissertations

The retinal pigment epithelium (RPE) is a monolayer of cells located directly behind the photoreceptor cells in the retina. These cells are involved in a variety of functions that support the visual process in the eye, namely 1) they form a blood-retina barrier which separates the neural retina from the choroid's blood supply, 2) the apical processes of RPE cells diurnally phagocytose the outer segments of photoreceptor cells, and 3) they participate in the renewal of the photopigment 11-cis retinal. Age-related macular degneration (AMD) is the leading cause of blindness in people over the age of 50 years in ...


Characterization Of Cucurbituril Complex Ions In The Gas Phase Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, Haizhen Zhang Nov 2006

Characterization Of Cucurbituril Complex Ions In The Gas Phase Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, Haizhen Zhang

Theses and Dissertations

Host-guest interactions have been well studied in the new century to obtain fundamental insights into supramolecular chemistry. Most of the pioneering works have been done using techniques such as NMR, X-ray crystallography, IR spectroscopy and so on. However, none of these techniques is universal for the investigation of all types of supramolecules, and usually they have one or more limiting factors such as relatively large sample consumption, matrix effects from solvents, etc. Electrospray mass spectrometry has been widely used to investigate host-guest interactions in the gas phase. A particular advantage of gas phase host-guest research is that the experimental results ...


Microfabrication And Evaluation Of Planar Thin-Film Microfluidic Devices, Bridget Ann Peeni Oct 2006

Microfabrication And Evaluation Of Planar Thin-Film Microfluidic Devices, Bridget Ann Peeni

Theses and Dissertations

Over the past 15 years, research in the field of microfluidics has rapidly gained popularity. By seeking to miniaturize and automate separation-based analysis, microfluidic research seeks to improve current methods through decreased cost, analysis time, and sources of contamination. My work has focused on developing a novel fabrication method, based on standard microfabrication techniques, to create thin-film microfluidic devices. This microfabrication format makes it possible to generate devices that provide high efficiencies, enable mass fabrication, and provide a platform capable of integrating the microfluidic and electronic components necessary for a micro-total analysis system (μ-TAS). Device fabrication combines the processes of ...


Detection Of Proteins By Two-Photon Excitation Of Native Fluorescence, Li Li Aug 2006

Detection Of Proteins By Two-Photon Excitation Of Native Fluorescence, Li Li

Theses and Dissertations

Proteins are of primary importance to the structure and function of all living cells. Study of proteins relies on the ability to separate a complex mixture so that individual proteins can be more easily processed by other techniques. Since protein samples often exist at low concentration in a small volume, the trend in chemical analysis is toward micro total analysis systems (µTAS) or lab-on-a-chip devices. Among µTAS separation methods, the relatively new electric field gradient focusing (EFGF) technique has shown potential. It focuses and separates analytes based on their electrophoretic migration in an opposing hydrodynamic flow. The detection principles that ...


Construction Of A Col11a1 Transgene Vector, Cameron Mckell Beck Aug 2006

Construction Of A Col11a1 Transgene Vector, Cameron Mckell Beck

Theses and Dissertations

Background: Cartilage disorders affect millions of people in the United States alone, with effects ranging from poor skeletal development and joint pain to shortened lifespan and perinatal lethality. Many of these disorders have their root in defects of collagen, type XI collagen being among the most important. A mouse model of such a type XI collagen defect is the chondrodysplasia (cho) mutant. Mice homozygous for this null mutation in the Col11a1 gene do not express the α1 chain of type XI collagen. This results in a functional knockout of type XI collagen, leading to insufficient skeletal development and perinatal lethality ...


The Effects Of Wearing A Cooling Vest During The Warm-Up On Long Distance Interval Training, Andrew Robert Tegeder Aug 2006

The Effects Of Wearing A Cooling Vest During The Warm-Up On Long Distance Interval Training, Andrew Robert Tegeder

Theses and Dissertations

Purpose: Reducing body temperature before exercise is called precooling. Past research suggests that reducing body core temperature (Tc) slightly can result in improved running performance. This study evaluated the effects that warming up, while wearing a cooling vest prior to an interval workout, had on Tc, and interval time in long-distance runners. Methods: Nineteen healthy male collegiate cross-country runners were recruited for this study. Each subject warmed up and exercised under two different conditions: (a) an experimental condition in which subjects wore a Nike PreCool® ice vest during warm-up and (b) a control condition. Subjects performed a warm-up followed by ...


Ferritin: Mechanistic Studies And Electron Transfer Properties, Bo Zhang Aug 2006

Ferritin: Mechanistic Studies And Electron Transfer Properties, Bo Zhang

Theses and Dissertations

Ferritins are ubiquitous iron storage proteins in living systems. Although much is known about the iron deposition process in ferritin and a mechanism has been developed, several important issues still remain unknown. One lingering question is the less than stoichiometric quantities of hydrogen peroxide detected in previous studies on animal ferritins. Extensive experimental data on identifying the species in competition for peroxide equivalents point to a surprising conclusion that H2O2 generated in the ferroxidase reaction is consumed by amine buffers that are commonly employed in in vitro ferritin studies, while non-nitrogen containing buffers, such as acetate, phosphate, and carbonate, do ...


The Phylogeography Of Prosopium In Western North America, Becky Akiko Miller Aug 2006

The Phylogeography Of Prosopium In Western North America, Becky Akiko Miller

Theses and Dissertations

The mountain whitefish (Prosopium williamsoni) has been largely overlooked in population genetic analyses despite its wide distribution in discrete drainage basins in western North America for over four million years. Its closest sister taxa the Bear Lake whitefish (P. abyssicola), Bonneville cisco (P. gemmifer), and Bonneville whitefish (P. spilonotus) are found only in Bear Lake Idaho-Utah and were also included in the analyses. A total of 1,334 cytochrome b and 1,371 NADH dehydrogenase subunit 2 sequences from the Bonneville Basin, the Columbia River Sub-basin, the lower Snake River Sub-basin, the upper Snake River Sub-basin, the Green River Basin ...


Contributions Of Muscle Fatigue To A Neuromuscular Neck Injury In Female Standard Ballroom Dancers, Teri Riding Aug 2006

Contributions Of Muscle Fatigue To A Neuromuscular Neck Injury In Female Standard Ballroom Dancers, Teri Riding

Theses and Dissertations

Objective: To investigate the potential etiology of a loss of neck control injury in female standard ballroom dancers. The median frequency (MF) as measured by electromyography (EMG) of the left upper trapezius (UT), left splenius capitius (SPL), and right sternocleidomastoid (SCM) of injured dancers was compared to non-injured dancers. This comparison was performed to identify whether dancers with a history of loss of neck control have a greater amount of fatigue than those with no history of this particular injury. Design and Setting: A 2 x 6 factorial design was used for this investigation. The independent variables were group (injured ...


Genetic Considerations For The Conservation And Management Of Yellowstone Cutthroat Trout (Oncorhynchus Clarkii Bouvieri) In Yellowstone National Park, David J. Janetski Aug 2006

Genetic Considerations For The Conservation And Management Of Yellowstone Cutthroat Trout (Oncorhynchus Clarkii Bouvieri) In Yellowstone National Park, David J. Janetski

Theses and Dissertations

A key component to conservation is an accurate understanding of genetic subdivision within a species. Despite their ecological and economic importance, relatively little is understood about the genetic structuring of Yellowstone cutthroat trout in Yellowstone National Park. Here, we use traditional (Fst, Rst, Nm, and AMOVA) and modern (Bayesian assignment tests, coalescent theory, and nested clade analysis) analytical approaches to describe the population genetic subdivision of cutthroat trout spawning populations in Yellowstone Lake and to identify genetically distinct population segments throughout Yellowstone National Park. Evidence for restricted gene flow between spawning populations within Yellowstone Lake was detected using nested clade ...


Classification Of Neuronal Nicotinic Acetylcholine Receptors In Rat Ca1 Hippocampal Interneuron Subpopulations Defined By Calcium-Binding Protein Mrna Expression, Richard M. Burgon Jul 2006

Classification Of Neuronal Nicotinic Acetylcholine Receptors In Rat Ca1 Hippocampal Interneuron Subpopulations Defined By Calcium-Binding Protein Mrna Expression, Richard M. Burgon

Theses and Dissertations

In this study, the single-cell relative quantitative mRNA expression of three Calcium-binding proteins (CaBPs; calbindin, calretinin, parvalbumin) and eight nicotinic acetylcholine receptor (nAChR) subunits (alpha2-alpha5, alpha7, beta2-beta4) from interneurons from the stratum radiatum or stratum oriens within the CA1 region of rat hippocampi was analyzed using quantitative real time RT-PCR. Eighty-seven percent of the interneurons examined expressed CaBP mRNA. Parvalbumin mRNA was detected in 64%, while calbindin and calretinin expression was detected in 26% and 40% of interneurons, respectively. CaBP expression was not exclusive; the average number of CaBP mRNA detected per interneuron of the 47 interneurons examined for CaBP ...


Predicting Maize Yield, Nutrient Concentration And Uptake In P And K Fertilized Soils: Pressurized Hot Water And Other Alternatives To Mehlich I Extraction In Guatemala Soils, Heather Mae Hunsaker Jul 2006

Predicting Maize Yield, Nutrient Concentration And Uptake In P And K Fertilized Soils: Pressurized Hot Water And Other Alternatives To Mehlich I Extraction In Guatemala Soils, Heather Mae Hunsaker

Theses and Dissertations

The inaccessibility and cost of soil testing reduce effectiveness of fertilizer use on small-scale subsistence farms, and inadequate funding promotes adoption of soil tests in developing countries with minimal validation. For example, Mehlich-I extraction of phosphorus (P) currently used extensively in Guatemala may not be suitable for its broad range of soils. At least four alternatives are available but are relatively untested [Bray 1, Mehlich III, Olsen and pressurized hot water (PHW)]. Pressurized hot water is relatively simple and inexpensive, but is not yet tested against other extraction methods under variable P or potassium (K) fertilization levels. To determine whether ...


Structure-Activity Studies Of Glycosphingolipids As Antigens Of Natural Killer T Cells, Randal Donald Goff Jul 2006

Structure-Activity Studies Of Glycosphingolipids As Antigens Of Natural Killer T Cells, Randal Donald Goff

Theses and Dissertations

Glycosphingolipids (GSLs), composed of a polar saccharide head and a lipophilic ceramide tail, are ubiquitous components of the plasma membrane of eukaryotic cells. They serve in many regulatory capacities and have antigenic properties towards natural killer T (NKT) cells of the innate immune system. Critical to the recognition of glycosylceramides by NKT cells are antigen presenting cells (APC), such as dendritic cells, which are responsible for binding, processing, and delivery of ligands to these lymphocytes. This event is mediated by CD1d, a major histocompatibility complex-like protein expressed on the surface of APCs, which binds GSL antigens by the ceramide moiety ...


Identification, Sequencing, Expression And Evolutionary Relationships Of The 11s Seed Storage Protein Gene In Chenopodium Quinoa Willd., Marie Renee Barrett Balzotti Jul 2006

Identification, Sequencing, Expression And Evolutionary Relationships Of The 11s Seed Storage Protein Gene In Chenopodium Quinoa Willd., Marie Renee Barrett Balzotti

Theses and Dissertations

Quinoa (Chenopodium quinoa) is an Andean crop adapted to harsh environmental conditions and containing a high and well-balanced protein composition. Two seed storage proteins, the 2S albumin and 11S globulin, are the major amino acid reservoirs for the developing seedling. An in-depth study of the genes encoding these proteins is necessary to understand the source of quinoa's protein quality. Our objectives include identification and sequencing of the 11S genomic and cDNA clones, analysis of 11S expression profiles in different quinoa accessions and evaluation of evolutionary relationships between the sequence of the 11S gene in quinoa and homologous genes in ...


Development Of A Notational Analysis System For Selected Soccer Skills Of A Women's College Team, Camille Thomas Jul 2006

Development Of A Notational Analysis System For Selected Soccer Skills Of A Women's College Team, Camille Thomas

Theses and Dissertations

The purposes of this study were to develop a notational system to evaluate passing, dribbling, first touch, and individual defensive skills as they relate to success during women's soccer games and to develop a statistical model to weight the importance of each skill on creating scoring opportunities. Sequences of skills in 10 Division I intercollegiate women's soccer games were coded using well defined performance scores and outcomes. The notational analysis system was highly reliable as demonstrated by high test-retest Spearman's correlations (>0.98) between the first and second notation of 3 games for all four skills. The ...