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Full-Text Articles in Life Sciences

Effect Of Oxidation, Ph, And Ionic Strength On Calpastatin Inhibition Of Μ- And M-Calpain, K. R. Maddock Carlin, Elisabeth J. Huff-Lonergan, L. J. Rowe, Steven M. Lonergan Apr 2006

Effect Of Oxidation, Ph, And Ionic Strength On Calpastatin Inhibition Of Μ- And M-Calpain, K. R. Maddock Carlin, Elisabeth J. Huff-Lonergan, L. J. Rowe, Steven M. Lonergan

Animal Science Publications

The objective of this study was to evaluate the effect of oxidation on μ- and m-calpain activity at varying pH and ionic strength conditions in the presence of calpastatin. In 2 separate experiments, purified porcine skeletal muscle μ- or m-calpain (0.45 units of caseinolytic activity) was incubated in the presence of calpastatin (0, 0.15, or 0.30 units) at pH 7.5, 6.5, or 6.0 with either 165 or 295 mM NaCl. The reactions were initiated with the addition of CaCl2 (100 μM for μ-calpain; 1 mM for m-calpain). In Experiment 1 ...


An Investigation Of Susceptibility To Porcine Reproductive And Respiratory Syndrome Virus Between Two Genetically Diverse Commercial Lines Of Pigs, Amy L. Vincent, Brad J. Thacker, Patrick G. Halbur, Max F. Rothschild, Eileen L. Thacker Jan 2006

An Investigation Of Susceptibility To Porcine Reproductive And Respiratory Syndrome Virus Between Two Genetically Diverse Commercial Lines Of Pigs, Amy L. Vincent, Brad J. Thacker, Patrick G. Halbur, Max F. Rothschild, Eileen L. Thacker

Animal Science Publications

The objective of this study was to determine whether host genetics play a role in susceptibility to the respiratory disease in growing pigs caused by the porcine reproductive and respiratory syndrome virus (PRRSV). Based on a previous study, 2 genetically diverse commercial lines of pigs that also were divergent in the susceptibility of monocyte-derived macrophages to PRRSV infection in vitro were selected for an in vivo challenge study. Based on the average percentage of infected macrophages for each line, a line derived from the Large White breed was characterized as fluorescence-activated cell sortinghi (FACShi), and a line derived ...


Thermal Tolerance Of Acid-Adapted And Non-Adapted Escherichia Coli O157:H7 And Salmonella In Ground Beef During Storage, Manpreet Singh, Shawna M. Simpson, Holly R. Mullins, James S. Dickson Jan 2006

Thermal Tolerance Of Acid-Adapted And Non-Adapted Escherichia Coli O157:H7 And Salmonella In Ground Beef During Storage, Manpreet Singh, Shawna M. Simpson, Holly R. Mullins, James S. Dickson

Animal Science Publications

Thermal tolerance of acid-adapted Escherichia coli O157:H7 or Salmonella in ground beef was evaluated during storage at 4°C or –20°C. Both pathogens were adapted to acidic conditions (pH ∼4.6) by growing in tryptic soy broth supplemented with 1% glucose. A five-strain cocktail of E. coli O157:H7 or Salmonella was grown separately in TSB (pH ∼6.6) and TSB + 1% glucose for 24 h at 37°C to provide cells with or without acid adaptation. Irradiated ground beef was inoculated with either acid-adapted or non-adapted E. coli O157:H7 or Salmonella; the samples stored at 4 ...


Genome-Wide Linkage Analysis To Identify Chromosomal Regions Affecting Phenotypic Traits In The Chicken. I. Growth And Average Daily Gain, H. Zhou, Nader Deeb, C. M. Evock-Clover, C. M. Ashwell, Susan J. Lamont Jan 2006

Genome-Wide Linkage Analysis To Identify Chromosomal Regions Affecting Phenotypic Traits In The Chicken. I. Growth And Average Daily Gain, H. Zhou, Nader Deeb, C. M. Evock-Clover, C. M. Ashwell, Susan J. Lamont

Animal Science Publications

Two informative chicken F2 populations based on crosses between a broiler breeder male line and dams from genetically distinct, highly inbred (>99%) chicken lines, the Leghorn G-B2 and Fayoumi M15.2, have been used for genome-wide linkage and QTL analysis. Phenotypic data on 12 body composition traits (breast muscle weight, breast muscle weight percentage, abdominal fat weight, abdominal fat weight percentage, heart weight, heart weight percentage, liver weight, liver weight percentage, spleen weight, spleen weight percentage, and drumstick weight, and drumstick weight percentage) were collected. Birds were genotyped for 269 microsatellite markers across the genome. The QTL Express program ...


Influence Of Harvest Processes On Pork Loin And Ham Quality, Matt A. Gardner, Elisabeth J. Huff-Lonergan, L. J. Rowe, Collette Schultz-Kaster, Steven M. Lonergan Jan 2006

Influence Of Harvest Processes On Pork Loin And Ham Quality, Matt A. Gardner, Elisabeth J. Huff-Lonergan, L. J. Rowe, Collette Schultz-Kaster, Steven M. Lonergan

Animal Science Publications

The purpose of this study was to determine the specific effects of extending the interval between dwell time and the duration of scalding on pork quality attributes. Sixty-four Duroc × Yorkshire pigs were randomly assigned to a 2 × 2 factorial treatment arrangement. Treatments included extending the dwell duration from 5 to 10 min and extending the scald duration from 5 to 8 min. All carcasses entered the cooler 50 min after exsanguination. At exsanguination, blood was collected for three 1-min intervals and then for a final 2-min period. Temperature and pH of the LM and semimembranosus muscle (SM) were measured at ...


Growth, Pork Quality, And Excretion Characteristics Of Pigs Fed Bt Corn Or Non-Transgenic Corn, M. G. Custodio, Wendy J. Powers, Elisabeth J. Huff-Lonergan, Marjorie A. Faust, Jeff Stein Jan 2006

Growth, Pork Quality, And Excretion Characteristics Of Pigs Fed Bt Corn Or Non-Transgenic Corn, M. G. Custodio, Wendy J. Powers, Elisabeth J. Huff-Lonergan, Marjorie A. Faust, Jeff Stein

Animal Science Publications

Two experiments (exp. 1 and exp. 2, respectively) were conducted to compare performance, pork quality, and excretion characteristics of pigs fed diets containing Bt 11 (Bt) or control corn (C: non-transgenic inbred lines for exp. 1, and a non-transgenic isoline for exp. 2). Experiment 1 involved barrows and gilts (total n = 64; initial BW = 64 kg and 60 kg), while in exp. 2, 120 barrows were used (initial BW = 17 kg). Pigs were allocated to pens, blocked by sex and BW. Isocaloric, isolysinic diets contained an indigestible marker in exp. 1. Feed disappearance and weight gain data, and excreta samples ...


Effects Of Available Dietary Carbohydrate And Preslaughter Treatment On Glycolytic Potential, Protein Degradation, And Quality Traits Of Pig Muscles, G. Bee, C. Biolley, G. Guex, W. Herzog, Steven M. Lonergan, Elisabeth J. Huff-Lonergan Jan 2006

Effects Of Available Dietary Carbohydrate And Preslaughter Treatment On Glycolytic Potential, Protein Degradation, And Quality Traits Of Pig Muscles, G. Bee, C. Biolley, G. Guex, W. Herzog, Steven M. Lonergan, Elisabeth J. Huff-Lonergan

Animal Science Publications

The current study was conducted to determine the interactive effects of a glycogen-reducing diet fed to finishing pigs and length of preslaughter transportion on muscle metabolic traits, proteolysis of intermediate filament and costameric proteins, and meat quality traits. Large White gilts and barrows (n = 48) were selected at 88 kg of BW and individually fed for 21 d a diet (2.6 kg/d) either high (HC) or low (LC) in available carbohydrates. Six gilts and 6 barrows fed the HC and LC diets were subjected to 0 or 3 h of transportation on the day of slaughter. Muscle temperature ...