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Open Access. Powered by Scholars. Published by Universities.®

2006

Genetics and Genomics

Microsatellites

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

New Polymorphic Microsatellites In Glossina Pallidipes (Diptera: Glossinidae) And Their Cross-Amplification In Other Tsetse Fly Taxa, J. O. Ouma, J. G. Marquez, E. S. Krafsur Oct 2006

New Polymorphic Microsatellites In Glossina Pallidipes (Diptera: Glossinidae) And Their Cross-Amplification In Other Tsetse Fly Taxa, J. O. Ouma, J. G. Marquez, E. S. Krafsur

Entomology Publications

We report the development and characterization of three new microsatellite markers in the tsetse fly, Glossina pallidipes (Diptera: Glossinidae). Fifty-eight alleles were scored in 192 individuals representing six natural populations. Allelic diversity ranged from 9 to 28 alleles per locus (mean 19.3 ± 5.5). Averaged across loci, observed heterozygosity was 0.581 ± 0.209, and expected heterozygosity was 0.619 ± 0.181. Cross-species amplifications of the G. pallidipesloci in other tsetse fly taxa are reported.


Molecular Genetic Variation Of Boll Weevil Populations In North America Estimated With Microsatellites: Implications For Patterns Of Dispersal, Kyung Seok Kim, Thomas W. Sappington May 2006

Molecular Genetic Variation Of Boll Weevil Populations In North America Estimated With Microsatellites: Implications For Patterns Of Dispersal, Kyung Seok Kim, Thomas W. Sappington

Entomology Publications

The boll weevil (Anthonomus grandis Boheman) is an insect pest of cotton that underwent a well-documented range expansion across the southeastern U.S. from Mexico beginning about 110 years ago. Eleven microsatellite loci were surveyed to infer the magnitude and pattern of genetic differentiation among boll weevil populations from 18 locations across eight U.S. states and northeast Mexico. Estimates of genetic diversity (allelic diversity and heterozygosity) were greater in Southern than Northern populations, and were greater in the west than the east among Northern populations. Boll weevil populations were genetically structured as a whole across the geographic range sampled ...


Microgeographical Breeding Structure Of The Tsetse Fly, Glossina Pallidipes In South‐Western Kenya, J. O. Ouma, J. G. Marquez, E. S. Krafsur Mar 2006

Microgeographical Breeding Structure Of The Tsetse Fly, Glossina Pallidipes In South‐Western Kenya, J. O. Ouma, J. G. Marquez, E. S. Krafsur

Entomology Publications

The origins of extant G. pallidipes Austen (Diptera: Glossinidae) populations in the ecologically well studied Lambwe and Nguruman valleys in Kenya are controversial because populations have recovered after seemingly effective attempts to achieve high levels of control. We investigated microgeographic breeding structure of the tsetse fly, Glossina pallidipes (Diptera: Glossinidae) by analyzing spatial and temporal variation at eight microsatellite loci to test hypotheses about endemism and immigration. Samples were obtained at seasonal intervals from trap sites separated by 200 m to 14 km and arranged into blocks. G. pallidipes populations nearest to Lambwe and Nguruman also were sampled. Spatial analysis ...