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Full-Text Articles in Life Sciences

Chironomid Hemoglobin Genetic Diversity As An Indicator Of The New Jersey Hackensack Meadowlands Wetland Health, Lene Marie De Coursin Jacobs Aug 2006

Chironomid Hemoglobin Genetic Diversity As An Indicator Of The New Jersey Hackensack Meadowlands Wetland Health, Lene Marie De Coursin Jacobs

Seton Hall University Dissertations and Theses (ETDs)

Kearny Marsh located within the Hackensack Meadowlands is a freshwater wetland impacted by multiple environmental stressors from human activity. The marsh is located in the northeastern New Jersey metropolitan area. Chironomid larvae, aquatic larvae of midge fly, were analyzed from two Kearny Marsh sample sites, W9 and W22. A previous sediment analysis and toxicity study characterized heavy metal contaminants the marsh. The data results were applied in this thesis study to determine the correlation between environmental stressors and chironomid genetic diversity. Site W9 sediment was impacted by severe levels of total heavy metal contamination, whereas site W22 had just below ...


In Vitro Expression And Purification Of Class I Mhc Molecules, Loi Cheng May 2006

In Vitro Expression And Purification Of Class I Mhc Molecules, Loi Cheng

Honors Scholar Theses

The major histocompatibility complex (MHC) is a gene family responsible for many critical functions of the immune system in most vertebrates. The MHC consists of three classes differentiated by their structure and function, and MHC class I encodes antigen binding proteins as well as chaperone and accessory proteins such as tapasin. The purpose of this project is to reconstitute several human MHC class I molecules in their peptide-filled and peptide-deficient forms, and to purify these proteins for biochemical study. The expressed proteins include wild type and mutant variants of the fusion protein human leukocyte antigen HLA-B*0801-fos, and human beta-2-microglobulin ...


Plasmid Probes For Screening Specific Genes In Large Fragment Dna Libraries, Leah Ferguson Apr 2006

Plasmid Probes For Screening Specific Genes In Large Fragment Dna Libraries, Leah Ferguson

Senior Honors Projects

Heliothis virescens, a member of the lepidopteran family, is of great importance to many people who rely on agriculture as a source of income because it is a pest of many important crops. Because H. virescens feeds on a broad array of hosts including cotton, tobacco, tomato, and soybean it has earned its place as a major nuisance in the United States. Heliothis subflexa is a close taxonomic relative of H. virescens, but the two species differ in that H. subflexa is a specialized herbivore that only feeds on plants in the genus Physalis. Studies have been performed to determine ...


Molecular Dissection Of Genomic Regions Influencing Important Economic Traits In The Pig , Antonio Marcos Costa Do Amaral Ramos Jan 2006

Molecular Dissection Of Genomic Regions Influencing Important Economic Traits In The Pig , Antonio Marcos Costa Do Amaral Ramos

Retrospective Theses and Dissertations

Recently, numerous genes and genomic regions associated with pork quality were identified. There is great potential for the pig industry in using information from molecular markers for the improvement of pork quality traits, such as color, marbling and pH. Besides the traditional fresh pork market, the production of dry-cured pork products, like Country Hams, has also increased. We collected data on fresh and dry-cured Country Hams to investigate the relationships between these traits and to analyze the effect of genetic markers on fresh and dry-cured ham processing characteristics. Significant positive correlations between yield and firmness, color, pH and marbling were ...


Use Of Qtl Identified In Breed Crosses For Genetic Improvement In Pigs, Napapan Piyasatian Jan 2006

Use Of Qtl Identified In Breed Crosses For Genetic Improvement In Pigs, Napapan Piyasatian

Retrospective Theses and Dissertations

Strategies for the exploitation of multiple QTL identified in breed crosses for genetic improvement of livestock were examined through marker-assisted introgression (MAI) and marker-assisted selection (MAS). Evaluation of the effectiveness of alternate strategies for MAI and MAS were carried out using stochastic computer simulation. In general, introgression of multiple QTL and maintaining the frequency of the donor's allele at 50% requires large population sizes that are not feasible in livestock. An alternate selection approach for MAI of multiple QTL was developed for a breeding program of limited size, without requiring the selected candidates to be heterozygous at all loci ...