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Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

2006

Genetics and Genomics

Articles

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Full-Text Articles in Life Sciences

Analysis Of Genetic Mutations In The 7a7b Open Reading Frame Of Coronavirus Of Cheetahs (Acinonyx Jubatus), Melissa Kennedy, E Moore, R Wilkes, S Citino, Stephen Kania Jan 2012

Analysis Of Genetic Mutations In The 7a7b Open Reading Frame Of Coronavirus Of Cheetahs (Acinonyx Jubatus), Melissa Kennedy, E Moore, R Wilkes, S Citino, Stephen Kania

Melissa A. Kennedy

OBJECTIVE: To analyze the 7a7b genes of the feline coronavirus (FCoV) of cheetahs, which are believed to play a role in virulence of this virus. SAMPLE POPULATION: Biologic samples collected during a 4-year period from 5 cheetahs at the same institution and at 1 time point from 4 cheetahs at different institutions. PROCEDURES: Samples were first screened for FCoV via a reverse transcription-PCR procedure involving primers that encompassed the 3'-untranslated region. Samples that yielded positive assay results were analyzed by use of primers that targeted the 7a7b open reading frames. The nucleotide sequences of the 7a7b amplification products were ...


Evaluation Of Canine Serum For The Presence Of Anti-Retinal Antibodies In Sudden Acquired Retinal Dengeneration Syndrome, R Keller, Stephen Kania, Diane Hendrix, Daniel Ward, K Abrams May 2011

Evaluation Of Canine Serum For The Presence Of Anti-Retinal Antibodies In Sudden Acquired Retinal Dengeneration Syndrome, R Keller, Stephen Kania, Diane Hendrix, Daniel Ward, K Abrams

Diane Hendrix

Objective: To determine the presence of serum antiretinal antibodies in sudden acquired retinal degeneration syndrome (SARDS) affected dogs and the size of the antigen to which these antibodies bind via the use of enzyme-linked immunosorbent assay (ELISA) and Western blot immunoassays. Animals studied: Serum was collected from 13 dogs affected by SARDS and five dogs with normal ocular examinations. Procedures: All serum samples were subjected to ELISA with saline-soluble canine retinal tissue and Western blot analyses with SDS solubilized normal canine retinal tissue as the antigen. Antirecoverin (23 kDa) and antiheat shock cognate (65 kDa) antibodies were used as positive ...


Evaluation Of Canine Serum For The Presence Of Anti-Retinal Antibodies In Sudden Acquired Retinal Dengeneration Syndrome, R Keller, Stephen Kania, Diane Hendrix, Daniel Ward, K Abrams Feb 2011

Evaluation Of Canine Serum For The Presence Of Anti-Retinal Antibodies In Sudden Acquired Retinal Dengeneration Syndrome, R Keller, Stephen Kania, Diane Hendrix, Daniel Ward, K Abrams

Daniel A. Ward

Objective: To determine the presence of serum antiretinal antibodies in sudden acquired retinal degeneration syndrome (SARDS) affected dogs and the size of the antigen to which these antibodies bind via the use of enzyme-linked immunosorbent assay (ELISA) and Western blot immunoassays. Animals studied: Serum was collected from 13 dogs affected by SARDS and five dogs with normal ocular examinations. Procedures: All serum samples were subjected to ELISA with saline-soluble canine retinal tissue and Western blot analyses with SDS solubilized normal canine retinal tissue as the antigen. Antirecoverin (23 kDa) and antiheat shock cognate (65 kDa) antibodies were used as positive ...