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Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

2006

Genetics and Genomics

Veterinary Microbiology and Preventive Medicine Publications

Virulence

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Full-Text Articles in Life Sciences

Virulence Genotyping Of Salmonella Spp. With Multiplex Pcr, Jerod A. Skyberg, Catherine M. Logue, Lisa K. Nolan Mar 2006

Virulence Genotyping Of Salmonella Spp. With Multiplex Pcr, Jerod A. Skyberg, Catherine M. Logue, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

The purpose of this study was to develop a multiplex polymerase chain reaction (PCR) protocol useful in the virulence genotyping of Salmonella spp. with the idea that genotyping could augment current Salmonella characterization and typing methods. Seventeen genes associated with Salmonella invasion, fimbrial production, toxin production, iron transport, and intramacrophage survival were targeted by three PCR reactions. Most of these genes are required for full Salmonella virulence in a murine model, and many are also located onSalmonella pathogenicity islands (PAIs) and are associated with type III secretion systems (TTSSs). Once the success of procedures that used positive and negative ...