Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

2006

Genetics and Genomics

Veterinary Microbiology and Preventive Medicine Publications

Salmonella

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Fitness Of Antimicrobial-Resistant Campylobacter And Salmonella, Qijing Zhang, Orhan Sahin, Patrick F. Mcdermott, Sophie Payot Jun 2006

Fitness Of Antimicrobial-Resistant Campylobacter And Salmonella, Qijing Zhang, Orhan Sahin, Patrick F. Mcdermott, Sophie Payot

Veterinary Microbiology and Preventive Medicine Publications

Campylobacter and Salmonella are the most commonly reported bacterial causes of human foodborne infections, and increasing proportions of these pathogens become resistant to medically important antimicrobial agents, imposing a burden on public health. Acquisition of resistance to antibiotics affects the adaptation and evolution of Salmonella and Campylobacter in various environments. Many resistance-conferring mutations entail a biological fitness cost, while others (e.g. fluoroquinolone resistance in Campylobacter) have no cost or even enhanced fitness. In Salmonella, the fitness disadvantage due to antimicrobial resistance can be restored by acquired compensatory mutations, which occur both in vitro and in vivo. The compensated or ...


Virulence Genotyping Of Salmonella Spp. With Multiplex Pcr, Jerod A. Skyberg, Catherine M. Logue, Lisa K. Nolan Mar 2006

Virulence Genotyping Of Salmonella Spp. With Multiplex Pcr, Jerod A. Skyberg, Catherine M. Logue, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

The purpose of this study was to develop a multiplex polymerase chain reaction (PCR) protocol useful in the virulence genotyping of Salmonella spp. with the idea that genotyping could augment current Salmonella characterization and typing methods. Seventeen genes associated with Salmonella invasion, fimbrial production, toxin production, iron transport, and intramacrophage survival were targeted by three PCR reactions. Most of these genes are required for full Salmonella virulence in a murine model, and many are also located onSalmonella pathogenicity islands (PAIs) and are associated with type III secretion systems (TTSSs). Once the success of procedures that used positive and negative ...