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Open Access. Powered by Scholars. Published by Universities.®

2006

Genetics and Genomics

Stephen A Kania

Articles 1 - 4 of 4

Full-Text Articles in Life Sciences

Oestrogen Receptor Evaluation In Pomeranian Dogs With Hair Cycle Arrest (Alopecia X) On Melatonin Supplementation, Linda Frank, Robert Donnell, Stephen Kania Dec 2005

Oestrogen Receptor Evaluation In Pomeranian Dogs With Hair Cycle Arrest (Alopecia X) On Melatonin Supplementation, Linda Frank, Robert Donnell, Stephen Kania

Stephen A Kania

The role of oestrogen receptors in dogs with hair cycle arrest (alopecia X) was investigated by immunohistochemistry. The purpose of this study was to determine if hair regrowth in dogs with hair cycle arrest treated with melatonin was associated with a decrease in follicular oestrogen receptors. Fifteen Pomeranians (excluding intact females) with hair cycle arrest were enrolled. Two biopsies were obtained from alopecic areas of the trunk before and after 3 months on melatonin. Haematoxylin and eosin-stained tissues were examined and oestrogen receptor-alpha was demonstrated immunohistochemically. Common histopathological findings included hyperkeratosis, follicular keratosis, excessive tricholemmal keratinization (flame follicles), thin epidermis ...


Analysis Of Genetic Mutations In The 7a7b Open Reading Frame Of Coronavirus Of Cheetahs (Acinonyx Jubatus), Melissa Kennedy, E Moore, R Wilkes, S Citino, Stephen Kania Dec 2005

Analysis Of Genetic Mutations In The 7a7b Open Reading Frame Of Coronavirus Of Cheetahs (Acinonyx Jubatus), Melissa Kennedy, E Moore, R Wilkes, S Citino, Stephen Kania

Stephen A Kania

OBJECTIVE: To analyze the 7a7b genes of the feline coronavirus (FCoV) of cheetahs, which are believed to play a role in virulence of this virus. SAMPLE POPULATION: Biologic samples collected during a 4-year period from 5 cheetahs at the same institution and at 1 time point from 4 cheetahs at different institutions. PROCEDURES: Samples were first screened for FCoV via a reverse transcription-PCR procedure involving primers that encompassed the 3'-untranslated region. Samples that yielded positive assay results were analyzed by use of primers that targeted the 7a7b open reading frames. The nucleotide sequences of the 7a7b amplification products were ...


Effects Of Colchicine And Oryzalin On Callus And Adventitious Shoot Formation Of Euphorbia Pulcherrima 'Winter Rose', K Pickens, (Max) Z Cheng, Stephen Kania Dec 2005

Effects Of Colchicine And Oryzalin On Callus And Adventitious Shoot Formation Of Euphorbia Pulcherrima 'Winter Rose', K Pickens, (Max) Z Cheng, Stephen Kania

Stephen A Kania

The mitotic inhibitors, colchicine and oryzalin, were evaluated for their effects on callus, adventitious shoot formation, and tetraploid induction of Euphorbia pulchurrima [Euphorbia pulcherrima] cv. Winter Rose. In vitro grown leaf sections were placed on various media supplemented with either colchicine or oryzalin at various concentrations for 1 to 4 days. Colchicine was less damaging to leaf tissues than oryzalin. On various colchicine-containing media, prolific calluses were produced and adventitious shoot formation was observed. Regenerated shoots were found to be diploid as determined by flow cytometry. On media supplemented with oryzalin (28.9 M to 144 M), leaf tissues produced ...


Evaluation Of Canine Serum For The Presence Of Anti-Retinal Antibodies In Sudden Acquired Retinal Dengeneration Syndrome, R Keller, Stephen Kania, Diane Hendrix, Daniel Ward, K Abrams Dec 2005

Evaluation Of Canine Serum For The Presence Of Anti-Retinal Antibodies In Sudden Acquired Retinal Dengeneration Syndrome, R Keller, Stephen Kania, Diane Hendrix, Daniel Ward, K Abrams

Stephen A Kania

Objective: To determine the presence of serum antiretinal antibodies in sudden acquired retinal degeneration syndrome (SARDS) affected dogs and the size of the antigen to which these antibodies bind via the use of enzyme-linked immunosorbent assay (ELISA) and Western blot immunoassays. Animals studied: Serum was collected from 13 dogs affected by SARDS and five dogs with normal ocular examinations. Procedures: All serum samples were subjected to ELISA with saline-soluble canine retinal tissue and Western blot analyses with SDS solubilized normal canine retinal tissue as the antigen. Antirecoverin (23 kDa) and antiheat shock cognate (65 kDa) antibodies were used as positive ...