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2006

Genetics and Genomics

James Van Etten Publications

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Chlorella Virus Mt325 Encodes Water And Potassium Channels That Interact Synergistically, Sabrina Gazzarrini, Ming Kang, Svetlana Epimashko, James L. Van Etten, Jack Dainty, Gerhard Thiel, Anna Moroni Feb 2006

Chlorella Virus Mt325 Encodes Water And Potassium Channels That Interact Synergistically, Sabrina Gazzarrini, Ming Kang, Svetlana Epimashko, James L. Van Etten, Jack Dainty, Gerhard Thiel, Anna Moroni

James Van Etten Publications

Fast and selective transport of water through cell membranes is facilitated by water channels. Water channels belonging to the major intrinsic proteins (MIPs) family have been found in all three domains of life, Archaea, Bacteria, and Eukarya. Here we show that Chlorella virus MT325 has a water channel gene, aqpv1, that forms a functional aquaglyceroporin in oocytes. aqpv1 is transcribed during infection together with MT325 kcv, a gene encoding a previously undescribed type of viral potassium channel. Coexpression of AQPV1 and MT325-Kcv in Xenopus oocytes synergistically increases water transport, suggesting a possible concerted action of the two channels in the ...


Chlorella Viruses, Takashi Yamada, Hideki Onimatsu, James L. Van Etten Jan 2006

Chlorella Viruses, Takashi Yamada, Hideki Onimatsu, James L. Van Etten

James Van Etten Publications

Chlorella viruses or chloroviruses are large, icosahedral, plaque‐forming, double‐stranded‐DNA— containing viruses that replicate in certain strains of the unicellular green alga Chlorella. DNA sequence analysis of the 330‐kbp genome of Paramecium bursaria chlorella virus 1 (PBCV‐1), the prototype of this virus family (Phycodnaviridae), predict ∼366 protein‐encoding genes and 11 tRNA genes. The predicted gene products of ∼50% of these genes resemble proteins of known function, including many that are completely unexpected for a virus. In addition, the chlorella viruses have several features and encode many gene products that distinguish them from most viruses. These ...