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Full-Text Articles in Life Sciences

Designing Sirna That Distinguish Between Genes That Differ By A Single Nucleotide, Dianne S. Schwarz, Hongliu Ding, Lori A. Kennington, Jessica T. Moore, Janell M. Schelter, Julja Burchard, Peter S. Linsley, Neil Aronin, Zuoshang Xu, Phillip D. Zamore Sep 2006

Designing Sirna That Distinguish Between Genes That Differ By A Single Nucleotide, Dianne S. Schwarz, Hongliu Ding, Lori A. Kennington, Jessica T. Moore, Janell M. Schelter, Julja Burchard, Peter S. Linsley, Neil Aronin, Zuoshang Xu, Phillip D. Zamore

GSBS Student Publications

Small interfering RNAs (siRNAs), the guides that direct RNA interference (RNAi), provide a powerful tool to reduce the expression of a single gene in human cells. Ideally, dominant, gain-of-function human diseases could be treated using siRNAs that specifically silence the mutant disease allele, while leaving expression of the wild-type allele unperturbed. Previous reports suggest that siRNAs can be designed with single nucleotide specificity, but no rational basis for the design of siRNAs with single nucleotide discrimination has been proposed. We systematically identified siRNAs that discriminate between the wild-type and mutant alleles of two disease genes: the human Cu, Zn superoxide ...


Epigenetic Telomere Protection By Drosophila Dna Damage Response Pathways, Sarah R. Oikemus, Joana Queiroz-Machado, Kuanju Lai, Nadine Schultz, Claudio E. Sunkel, Michael H. Brodsky May 2006

Epigenetic Telomere Protection By Drosophila Dna Damage Response Pathways, Sarah R. Oikemus, Joana Queiroz-Machado, Kuanju Lai, Nadine Schultz, Claudio E. Sunkel, Michael H. Brodsky

GSBS Student Publications

Analysis of terminal deletion chromosomes indicates that a sequence-independent mechanism regulates protection of Drosophila telomeres. Mutations in Drosophila DNA damage response genes such as atm/tefu, mre11, or rad50 disrupt telomere protection and localization of the telomere-associated proteins HP1 and HOAP, suggesting that recognition of chromosome ends contributes to telomere protection. However, the partial telomere protection phenotype of these mutations limits the ability to test if they act in the epigenetic telomere protection mechanism. We examined the roles of the Drosophila atm and atr-atrip DNA damage response pathways and the nbs homolog in DNA damage responses and telomere protection. As ...