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Full-Text Articles in Life Sciences

The Role Of Ilv5p Interacting Factors In Mitochondrial Dna Stability, Anthony J. Mirando Jun 2006

The Role Of Ilv5p Interacting Factors In Mitochondrial Dna Stability, Anthony J. Mirando

Biology Master’s Theses

The ease of manipulating yeast allows for advanced studies on the factors affecting the mitochondrial DNA mutation rates. The control mechanisms of the mitochondrial DNA mutation rate has been determined to involve the dual function protein, Ilv5p. The Ilv5p plays an integral role in the proper segregation of newly replicated mitochondrial DNA into daughter cells during cell division. The focus of this study is to find unknown factors involved in mitochondrial DNA stability. This study uses the Ilv5p to pull unknown factors out of the many genes that comprise the yeast genome. The identification of interacting factors of the Ilv5p ...


Evaluation Of Gyp7 Protein Ability To Coordinate And Regulate Mitochondrial Genomes Stability, Louis Didone Jun 2006

Evaluation Of Gyp7 Protein Ability To Coordinate And Regulate Mitochondrial Genomes Stability, Louis Didone

Biology Master’s Theses

Cellular creation of adenosine triphosphate, ATP, is essential for eukaryotic cells to function properly. The ATP molecule drives most of the biochemical and metabolic pathways of the cell. The cell's ATP is produced in the mitochondria. Mutations within the genome of the mitochondria will alter the cell's ability to generate A TP. Preliminary work has shown that loss of the Gyp 7p in Saccharomyces cerevisiae blocks the ability of mitochondria to properly function. The Gyp 7 gene was isolated using a technique called two-hybrid analysis with a known mitochondrial protein called llvSp, which was used as 'bait'. We ...


Characterization Of Fmp35: A Novel Gene And Its Role In Mitochondrial Dna Stability, Chad A. Cornelius Jan 2006

Characterization Of Fmp35: A Novel Gene And Its Role In Mitochondrial Dna Stability, Chad A. Cornelius

Biology Master’s Theses

Mitochondria are essential organelles for all eukaryotic organisms with very few exceptions. The life-giving processes contributed by mitochondria are the end result of many proteins that are encoded within the mitochondria. Many nuclear encoded proteins give mitochondrial DNA (mtDNA) the high stability needed so that life can thrive.

Saccharomyces cerevisiae (baker's yeast) has historically been a model organism for mitochondrial function studies. These yeast are categorized as facultative anarobes; meaning that they are able to respire or ferment depending on media available. Functional mitochondria allow baker's yeast to thrive on a 3-carbon medium (p+), while mitochondrial dysfunctions due ...