Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

2006

Genetics and Genomics

Iowa State University

Keyword
Publication
Publication Type

Articles 1 - 30 of 67

Full-Text Articles in Life Sciences

Analysis Of Bulked And Redundant Accessions Of Brassica Germplasm Using Assignment Tests Of Microsatellite Markers, Von Mark V. Cruz, John D. Nason, Richard Luhman, Laura F. Marek, Randy C. Shoemaker, E. Charles Brummer, Candice A. C. Gardner Dec 2006

Analysis Of Bulked And Redundant Accessions Of Brassica Germplasm Using Assignment Tests Of Microsatellite Markers, Von Mark V. Cruz, John D. Nason, Richard Luhman, Laura F. Marek, Randy C. Shoemaker, E. Charles Brummer, Candice A. C. Gardner

Ecology, Evolution and Organismal Biology Publications

This study was conducted to determine if Brassica germplasm bulks created and maintained by the USDA-ARS North Central Plant Introduction Station (NCRPIS) were made with genetically indistinguishable component accessions and to examine newly identified putative duplicate accessions to determine if they can be bulked. Using ten microsatellite primer pairs, we genotyped two bulks of B. rapa L. ssp. dichotoma (Roxb.) Hanelt comprising four accessions and three bulks of B. rapa L. ssp. trilocularis (Roxb.) Hanelt comprising fourteen accessions, as well as four pairs of putatively duplicate accessions of B.␣napus L. Assignment tests on ten individual plants per accession were ...


Complete Dna Sequence, Comparative Genomics, And Prevalence Of An Inchi2 Plasmid Occurring Among Extraintestinal Pathogenic Escherichia Coli Isolates, Timothy J. Johnson, Yvonne Wannemuehler, Jennifer A. Scaccianoce, Sara J. Johnson, Lisa K. Nolan Nov 2006

Complete Dna Sequence, Comparative Genomics, And Prevalence Of An Inchi2 Plasmid Occurring Among Extraintestinal Pathogenic Escherichia Coli Isolates, Timothy J. Johnson, Yvonne Wannemuehler, Jennifer A. Scaccianoce, Sara J. Johnson, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

We have sequenced a large plasmid that occurs among avian pathogenicEscherichia coli isolates. This plasmid, pAPEC-O1-R, is a 241,387-bp IncHI2 plasmid which is cotransmissible via bacterial conjugation with a ColBM virulence plasmid, encodes resistance to eight antimicrobial agents, and appears to occur at low rates among extraintestinal E. coli isolates.


Dispersal Of Newly Eclosed European Corn Borer Adults (Lepidoptera: Crambidae) From Corn Into Small-Grain Aggregation Plots, Brendon James Reardon, Douglas V. Sumerford, Thomas W. Sappington Oct 2006

Dispersal Of Newly Eclosed European Corn Borer Adults (Lepidoptera: Crambidae) From Corn Into Small-Grain Aggregation Plots, Brendon James Reardon, Douglas V. Sumerford, Thomas W. Sappington

Entomology Publications

Genetically modified, insecticidal Bacillus thuringiensis (Bt) corn, Zea mays L., hybrids are used throughout the Corn Belt for European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), control. To slow development of Bt corn resistance, the Environmental Protection Agency requires growers to plant a refuge. Determining the appropriate distance between a refuge and Bt corn, and development of mitigation–remediation strategies such as mass releases of susceptible moths, requires an understanding of adult dispersal and mating behavior. However, much remains unknown about these behaviors. Because mating often occurs in grass near cornfields where adult O. nubilalis aggregate, we planted small-grain plots ...


New Polymorphic Microsatellites In Glossina Pallidipes (Diptera: Glossinidae) And Their Cross-Amplification In Other Tsetse Fly Taxa, J. O. Ouma, J. G. Marquez, E. S. Krafsur Oct 2006

New Polymorphic Microsatellites In Glossina Pallidipes (Diptera: Glossinidae) And Their Cross-Amplification In Other Tsetse Fly Taxa, J. O. Ouma, J. G. Marquez, E. S. Krafsur

Entomology Publications

We report the development and characterization of three new microsatellite markers in the tsetse fly, Glossina pallidipes (Diptera: Glossinidae). Fifty-eight alleles were scored in 192 individuals representing six natural populations. Allelic diversity ranged from 9 to 28 alleles per locus (mean 19.3 ± 5.5). Averaged across loci, observed heterozygosity was 0.581 ± 0.209, and expected heterozygosity was 0.619 ± 0.181. Cross-species amplifications of the G. pallidipesloci in other tsetse fly taxa are reported.


Differential Lineage-Specific Amplification Of Transposable Elements Is Responsible For Genome Size Variation In Gossypium, Jennifer S. Hawkins, Hyeran Kim, John D. Nason, Rod A. Wing, Jonathan F. Wendel Oct 2006

Differential Lineage-Specific Amplification Of Transposable Elements Is Responsible For Genome Size Variation In Gossypium, Jennifer S. Hawkins, Hyeran Kim, John D. Nason, Rod A. Wing, Jonathan F. Wendel

Ecology, Evolution and Organismal Biology Publications

The DNA content of eukaryotic nuclei (C-value) varies ∼200,000-fold, but there is only a ∼20-fold variation in the number of protein-coding genes. Hence, most C-value variation is ascribed to the repetitive fraction, although little is known about the evolutionary dynamics of the specific components that lead to genome size variation. To understand the modes and mechanisms that underlie variation in genome composition, we generated sequence data from whole genome shotgun (WGS) libraries for three representative diploid (n = 13) members of Gossypium that vary in genome size from 880 to 2460 Mb (1C) and from a phylogenetic outgroup, Gossypioides kirkii ...


Transposition Of Reversed Ac Element Ends Generates Novel Chimeric Genes In Maize, Jianbo Zhang, Feng Zhang, Thomas Peterson Oct 2006

Transposition Of Reversed Ac Element Ends Generates Novel Chimeric Genes In Maize, Jianbo Zhang, Feng Zhang, Thomas Peterson

Genetics, Development and Cell Biology Publications

The maize Activator/Dissociation (Ac/Ds) elements are members of the hAT (hobo, Ac, and Tam3) superfamily of type II (DNA) transposons that transpose through a “cut-and-paste” mechanism. Previously, we reported that a pair of Ac ends in reversed orientation is capable of undergoing alternative transposition reactions that can generate large-scale chromosomal rearrangements, including deletions and inversions. We show here that rearrangements induced by reversed Ac ends transposition can join the coding and regulatory sequences of two linked paralogous genes to generate a series of chimeric genes, some of which are functional. To our knowledge, this is the first report ...


Occurrence Of Pathogenicity Island Iapec-O1 Genes Among Escherichia Coli Implicated In Avian Colibacillosis, Subhashinie Kariyawasam, Timothy J. Johnson, Chitrita Debroy, Lisa K. Nolan Sep 2006

Occurrence Of Pathogenicity Island Iapec-O1 Genes Among Escherichia Coli Implicated In Avian Colibacillosis, Subhashinie Kariyawasam, Timothy J. Johnson, Chitrita Debroy, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is a leading cause of economic loss to the poultry industry worldwide. The ability of APEC to cause disease is determined by certain virulence markers, some of which are located on pathogenicity islands (PAIs). We recently described one such PAI in an APEC O1:K1 strain (APEC-O1). This PAI, termed PAI IAPEC-O1, carries the genes of the pap operon, a region similar to the tiainvasion determinant of enterotoxigenic E. coli; ireA, a gene that encodes an iron-responsive element; and a novel 1.5-kb region, ORF 54. Here, the occurrence of ...


Estimating The Number Of True Null Hypotheses From A Histogram Of P Values, Dan Nettleton, J.T. Gene Hwang, Rico A. Caldo, Roger P. Wise Sep 2006

Estimating The Number Of True Null Hypotheses From A Histogram Of P Values, Dan Nettleton, J.T. Gene Hwang, Rico A. Caldo, Roger P. Wise

Statistics Publications

In an earlier article, an intuitively appealing method for estimating the number of true null hypotheses in a multiple test situation was proposed. That article presented an iterative algorithm that relies on a histogram of observed p values to obtain the estimator. We characterize the limit of that iterative algorithm and show that the estimator can be computed directly without iteration. We compare the performance of the histogram-based estimator with other procedures for estimating the number of true null hypotheses from a collection of observed p values and find that the histogram-based estimator performs well in settings similar to those ...


Complete Dna Sequence Of A Colbm Plasmid From Avian Pathogenic Escherichia Coli Suggests That It Evolved From Closely Related Colv Virulence Plasmids, Timothy J. Johnson, Sara J. Johnson, Lisa K. Nolan Aug 2006

Complete Dna Sequence Of A Colbm Plasmid From Avian Pathogenic Escherichia Coli Suggests That It Evolved From Closely Related Colv Virulence Plasmids, Timothy J. Johnson, Sara J. Johnson, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

Avian pathogenic Escherichia coli (APEC), an extraintestinal pathogenic E. colicausing colibacillosis in birds, is responsible for significant economic losses for the poultry industry. Recently, we reported that the APEC pathotype was characterized by possession of a set of genes contained within a 94-kb cluster linked to a ColV plasmid, pAPEC-O2-ColV. These included sitABCD, genes of the aerobactin operon, hlyF, iss, genes of the salmochelin operon, and the 5′ end ofcvaB of the ColV operon. However, the results of gene prevalence studies performed among APEC isolates revealed that these traits were not always linked to ColV plasmids. Here, we ...


Using Genetic Markers And Population Assignment Techniques To Infer Origin Of Boll Weevils (Coleoptera: Curculionidae) Unexpectedly Captured Near An Eradication Zone In Mexico, Kyung Seok Kim, Pedro Cano-Rios, Thomas W. Sappington Aug 2006

Using Genetic Markers And Population Assignment Techniques To Infer Origin Of Boll Weevils (Coleoptera: Curculionidae) Unexpectedly Captured Near An Eradication Zone In Mexico, Kyung Seok Kim, Pedro Cano-Rios, Thomas W. Sappington

Entomology Publications

Several boll weevils, Anthonomus grandis Boheman, were captured in pheromone traps in 2004 near Tlahualilo, Durango, Mexico, an area where none had been reported for ≈10 yr. It is possible that they were from an endemic population normally too low in numbers to be detected but that increased in response to more favorable rainfall conditions in 2004. Alternatively, they may represent an influx of migrants or the immediate descendents of migrants. To identify the most likely origin of the boll weevils captured in this area, we characterized microsatellite variation of the Tlahualilo weevils and compared it with the variation from ...


Glossina Swynnertoni (Diptera: Glossinidae): Effective Population Size And Breeding Structure Estimated By Mitochondrial Diversity, J. G. Marquez, I. I. Malele, J. O. Ouma, E. S. Krafsur Aug 2006

Glossina Swynnertoni (Diptera: Glossinidae): Effective Population Size And Breeding Structure Estimated By Mitochondrial Diversity, J. G. Marquez, I. I. Malele, J. O. Ouma, E. S. Krafsur

Entomology Publications

Nucleotide diversity was examined at mitochondrial COI and r16S2 loci in eight Glossina swynnertoni Austen collections from northern Tanzania and from a culture maintained by the International Atomic Energy Agency. Eighteen composite haplotypes were observed among 149 flies, two of which were common to all samples and 10 were private. Mean haplotype diversity was 0.59 and nucleotide diversity was 0.0013. There were excess singular haplotypes and mutation-drift disequilibrium suggesting that populations had experienced an earlier bottleneck and subsequent expansion. Factorial correspondence analysis showed that haplotype frequencies varied much more temporally (GST = 0.18) than spatially (GST = 0.04 ...


Scanning Microarrays At Multiple Intensities Enhances Discovery Of Differentially Expressed Genes, David S. Skibbe, Xiujuan Wang, Xuefeng Zhao, Lisa A. Borsuk, Dan Nettleton, Patrick S. Schnable Aug 2006

Scanning Microarrays At Multiple Intensities Enhances Discovery Of Differentially Expressed Genes, David S. Skibbe, Xiujuan Wang, Xuefeng Zhao, Lisa A. Borsuk, Dan Nettleton, Patrick S. Schnable

Statistics Publications

Motivation: Scanning parameters are often overlooked when optimizing microarray experiments. A scanning approach that extends the dynamic data range by acquiring multiple scans of different intensities has been developed.

Results: Data from each of three scan intensities (low, medium, high) were analyzed separately using multiple scan and linear regression approaches to identify and compare the sets of genes that exhibit statistically significant differential expression. In the multiple scan approach only one-third of the differentially expressed genes were shared among the three intensities, and each scan intensity identified unique sets of differentially expressed genes. The set of differentially expressed genes from ...


Coordinated Diabrotica Genetics Research: Accelerating Progress On An Urgent Insect Pest Problem, Thomas W. Sappington, Blair D. Siegfried, Thomas Guillemaud Jul 2006

Coordinated Diabrotica Genetics Research: Accelerating Progress On An Urgent Insect Pest Problem, Thomas W. Sappington, Blair D. Siegfried, Thomas Guillemaud

Entomology Publications

No abstract provided.


Gene Expression Profiling In Salmonella Choleraesuis-Infected Porcine Lung Using A Long Oligonucleotide Microarray, Shu-Hong Zhao, Daniel Kuhar, Joan K. Lunney, Harry Dawson, Catherine Guidry, Jolita J. Uthe, Shawn M. D. Bearson, Justin Recknor, Dan Nettleton, Christopher K. Tuggle Jul 2006

Gene Expression Profiling In Salmonella Choleraesuis-Infected Porcine Lung Using A Long Oligonucleotide Microarray, Shu-Hong Zhao, Daniel Kuhar, Joan K. Lunney, Harry Dawson, Catherine Guidry, Jolita J. Uthe, Shawn M. D. Bearson, Justin Recknor, Dan Nettleton, Christopher K. Tuggle

Statistics Publications

Understanding the transcriptional response to pathogenic bacterial infection within food animals is of fundamental and applied interest. To determine the transcriptional response to Salmonella enterica serovar Choleraesuis (SC) infection, a 13,297-oligonucleotide swine array was used to analyze RNA from control, 24-h postinoculation (hpi), and 48-hpi porcine lung tissue from pigs infected with SC. In total, 57 genes showed differential expression (p < 0.001; false discovery rate = 12%). Quantitative real-time PCR (qRT-PCR) of 61 genes was used to confirm the microarray results and to identify pathways responding to infection. Of the 33 genes identified by microarray analysis as differentially expressed, 23 were confirmed by qRT-PCR results. A novel finding was that two transglutaminase family genes (TGM1 and TGM3) showed dramatic increases in expression postinoculation; combined with several other apoptotic genes, they indicated the induction of apoptotic pathways during SC infection. A predominant T helper 1-type immune response occurred during infection, with interferon γ ...


Evaluating Binding Affinities By Force Stratification And Force Panning, Eric Henderson, Curtis Mosher Jun 2006

Evaluating Binding Affinities By Force Stratification And Force Panning, Eric Henderson, Curtis Mosher

Genetics, Development and Cell Biology Patents

The present invention is a method for selectively removing objects from a surface utilizing a probe. The probe is scanned over the surface utilizing a greater and greater relative amount of force so that a certain number of the objects are removed from the surface. The force required to remove the objects from the surface can be calculated utilizing Hook's law and the spring constant of the probe. After removal of the objects that have a relatively weaker binding affinity with the surface, the remaining objects can be harvested, characterized, and subjected to further study.


Genetic Aspects Of Female Longevity, S. J. Moeller, K. J. Stalder Jun 2006

Genetic Aspects Of Female Longevity, S. J. Moeller, K. J. Stalder

Animal Science White Papers, Technical Reports, & Fact Sheets

Longevity of the breeding female has both economic and animal welfare implications for the swine industry. High culling levels lead to increased replacement rates and reduce lifetime productivity for individual breeding females and the breeding herd. Greater replacement rates increase production costs related to purchase (or development), isolation, and acclimation of replacement females. PigCHAMP [1-5] summaries (Table 1) from 1998 through 2003, report annualized replacement rates of > 50% and average herd parity of only 2.5 litters. Breeding herd productivity and economic sustainability are compromised when females are culled early in life and prior to achieving a positive return on ...


Fitness Of Antimicrobial-Resistant Campylobacter And Salmonella, Qijing Zhang, Orhan Sahin, Patrick F. Mcdermott, Sophie Payot Jun 2006

Fitness Of Antimicrobial-Resistant Campylobacter And Salmonella, Qijing Zhang, Orhan Sahin, Patrick F. Mcdermott, Sophie Payot

Veterinary Microbiology and Preventive Medicine Publications

Campylobacter and Salmonella are the most commonly reported bacterial causes of human foodborne infections, and increasing proportions of these pathogens become resistant to medically important antimicrobial agents, imposing a burden on public health. Acquisition of resistance to antibiotics affects the adaptation and evolution of Salmonella and Campylobacter in various environments. Many resistance-conferring mutations entail a biological fitness cost, while others (e.g. fluoroquinolone resistance in Campylobacter) have no cost or even enhanced fitness. In Salmonella, the fitness disadvantage due to antimicrobial resistance can be restored by acquired compensatory mutations, which occur both in vitro and in vivo. The compensated or ...


Gene Expression Programs During Shoot, Root, And Callus Development In Arabidopsis Tissue Culture, Ping Che, Sonia Lall, Dan Nettleton, Stephen H. Howell Jun 2006

Gene Expression Programs During Shoot, Root, And Callus Development In Arabidopsis Tissue Culture, Ping Che, Sonia Lall, Dan Nettleton, Stephen H. Howell

Statistics Publications

Shoots can be regenerated from Arabidopsis (Arabidopsis thaliana) root explants in tissue culture through a two-step process requiring preincubation on an auxin-rich callus induction medium. Regenerating tissues can be directed along different developmental pathways leading to the formation of shoots, new roots, or callus by transferring to the appropriate organ induction medium. Using gene-profiling methods, we identified groups of genes that serve as molecular signatures of the different developmental processes, i.e. genes that were specifically up- or down-regulated on one developmental pathway, but not on others. One transcription factor gene that was up-regulated during early shoot development was RAP2 ...


Shared Microsatellite Loci In Glossina Morsitans Sensu Lato (Diptera: Glossinidae), E. S. Krafsur, M. A. Endsley May 2006

Shared Microsatellite Loci In Glossina Morsitans Sensu Lato (Diptera: Glossinidae), E. S. Krafsur, M. A. Endsley

Entomology Publications

Estimation of allelic frequencies at three microsatellite loci among 20 populations of Glossina morsitans morsitans Westwood, Glossina morsitans submorsitans Newstead, and Glossina morsitans centralis Machado indicated only two of 99 alleles were shared between three subspecies and 18 between any two subspecies; 81 alleles were unshared. The conserved flanking regions of each locus were completely shared. Genetic differentiation among subspecies, based on allele size, was RST = 0.87, close to the theoretic maximum value. All evidence suggests longstanding and complete reproductive isolation in nature among the sibling species. They should be elevated to specific rank.


A Root-Knot Nematode Secretory Peptide Functions As A Ligand For A Plant Transcription Factor, Guozhong Huang, Ruihua Dong, Rex Allen, Eric L. Davis, Thomas J. Baum, Richard S. Hussey May 2006

A Root-Knot Nematode Secretory Peptide Functions As A Ligand For A Plant Transcription Factor, Guozhong Huang, Ruihua Dong, Rex Allen, Eric L. Davis, Thomas J. Baum, Richard S. Hussey

Plant Pathology and Microbiology Publications

Parasitism genes expressed in the esophageal gland cells of root-knot nematodes encode proteins that are secreted into host root cells to transform the recipient cells into enlarged multinucleate feeding cells called giant-cells. Expression of a root-knot nematode parasitism gene which encodes a novel 13-amino-acid secretory peptide in plant tissues stimulated root growth. Two SCARECROW-like transcription factors of the GRAS protein family were identified as the putative targets for this bioactive nematode peptide in yeast two-hybrid analyses and confirmed by in vitro and in vivo coimmunoprecipitations. This discovery is the first demonstration of a direct interaction of a nematode-secreted parasitism peptide ...


Molecular Genetic Variation Of Boll Weevil Populations In North America Estimated With Microsatellites: Implications For Patterns Of Dispersal, Kyung Seok Kim, Thomas W. Sappington May 2006

Molecular Genetic Variation Of Boll Weevil Populations In North America Estimated With Microsatellites: Implications For Patterns Of Dispersal, Kyung Seok Kim, Thomas W. Sappington

Entomology Publications

The boll weevil (Anthonomus grandis Boheman) is an insect pest of cotton that underwent a well-documented range expansion across the southeastern U.S. from Mexico beginning about 110 years ago. Eleven microsatellite loci were surveyed to infer the magnitude and pattern of genetic differentiation among boll weevil populations from 18 locations across eight U.S. states and northeast Mexico. Estimates of genetic diversity (allelic diversity and heterozygosity) were greater in Southern than Northern populations, and were greater in the west than the east among Northern populations. Boll weevil populations were genetically structured as a whole across the geographic range sampled ...


Nanoscale Molecular Arrayer, Eric Henderson, Curtis Mosher Mar 2006

Nanoscale Molecular Arrayer, Eric Henderson, Curtis Mosher

Genetics, Development and Cell Biology Patents

The present invention is a dedicated apparatus for the formation of array that includes one or more deposition domains comprised of one or more deposition materials. The present invention may include an X, Y controller, an X, Y translation stage, a loading substrate, a deposition substrate, a Z controller, and a deposition probe. A computer controls all of the relative positions of each of the components. Furthermore, the present invention utilizes a humidity control system to create a capillary bridge between the probe and the substrate for transferring the deposition material between the loading substrate, the deposition probe, and the ...


Virulence Genotyping Of Salmonella Spp. With Multiplex Pcr, Jerod A. Skyberg, Catherine M. Logue, Lisa K. Nolan Mar 2006

Virulence Genotyping Of Salmonella Spp. With Multiplex Pcr, Jerod A. Skyberg, Catherine M. Logue, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

The purpose of this study was to develop a multiplex polymerase chain reaction (PCR) protocol useful in the virulence genotyping of Salmonella spp. with the idea that genotyping could augment current Salmonella characterization and typing methods. Seventeen genes associated with Salmonella invasion, fimbrial production, toxin production, iron transport, and intramacrophage survival were targeted by three PCR reactions. Most of these genes are required for full Salmonella virulence in a murine model, and many are also located onSalmonella pathogenicity islands (PAIs) and are associated with type III secretion systems (TTSSs). Once the success of procedures that used positive and negative ...


Microgeographical Breeding Structure Of The Tsetse Fly, Glossina Pallidipes In South‐Western Kenya, J. O. Ouma, J. G. Marquez, E. S. Krafsur Mar 2006

Microgeographical Breeding Structure Of The Tsetse Fly, Glossina Pallidipes In South‐Western Kenya, J. O. Ouma, J. G. Marquez, E. S. Krafsur

Entomology Publications

The origins of extant G. pallidipes Austen (Diptera: Glossinidae) populations in the ecologically well studied Lambwe and Nguruman valleys in Kenya are controversial because populations have recovered after seemingly effective attempts to achieve high levels of control. We investigated microgeographic breeding structure of the tsetse fly, Glossina pallidipes (Diptera: Glossinidae) by analyzing spatial and temporal variation at eight microsatellite loci to test hypotheses about endemism and immigration. Samples were obtained at seasonal intervals from trap sites separated by 200 m to 14 km and arranged into blocks. G. pallidipes populations nearest to Lambwe and Nguruman also were sampled. Spatial analysis ...


Detection And Quantification Of Protein Biomarkers From Fewer Than 10 Cells, Saju Nettikadan, Korinna Radke, James Johnson, Juntao Xu, Michael Lynch, Curtis Mosher, Eric Henderson Feb 2006

Detection And Quantification Of Protein Biomarkers From Fewer Than 10 Cells, Saju Nettikadan, Korinna Radke, James Johnson, Juntao Xu, Michael Lynch, Curtis Mosher, Eric Henderson

Genetics, Development and Cell Biology Publications

The use of antibody microarrays continues to grow rapidly due to the recent advances in proteomics and automation and the opportunity this combination creates for high throughput multiplexed analysis of protein biomarkers. However, a primary limitation of this technology is the lack of PCR-like amplification methods for proteins. Therefore, to realize the full potential of array-based protein biomarker screening it is necessary to construct assays that can detect and quantify protein biomarkers with very high sensitivity, in the femtomolar range, and from limited sample quantities. We describe here the construction of ultramicroarrays, combining the advantages of microarraying including multiplexing capabilities ...


Method And Apparatus For Solid State Molecular Analysis, Eric Henderson, Curtis Mosher, Michael P. Lynch Feb 2006

Method And Apparatus For Solid State Molecular Analysis, Eric Henderson, Curtis Mosher, Michael P. Lynch

Genetics, Development and Cell Biology Patents

The invention is a method for the formation and analysis of novel miniature deposition domains. These deposition domains are placed on a surface to form a molecular array. The molecular array is scanned with an AFM to analyze molecular recognition events and the effect of introduced agents on defined molecular interactions. This approach can be carried out in a high throughput format, allowing rapid screening of thousands of molecular species in a solid state array. The procedures described here have the added benefit of allowing the measurement of changes in molecular binding events resulting from changes in the analysis environment ...


Dispersal Of Boll Weevils (Coleoptera: Curculionidae) From Cotton Modules Before Ginning, Thomas W. Sappington, Mark D. Arnold, Alan D. Brashears, Megha N. Parajulee, Stanley C. Carroll, Allen E. Knutson, John W. Norman Jr. Feb 2006

Dispersal Of Boll Weevils (Coleoptera: Curculionidae) From Cotton Modules Before Ginning, Thomas W. Sappington, Mark D. Arnold, Alan D. Brashears, Megha N. Parajulee, Stanley C. Carroll, Allen E. Knutson, John W. Norman Jr.

Entomology Publications

We characterized the level of risk of boll weevil, Anthonomus grandis grandis Boheman, reintroduction to an eradication zone posed by dispersal from cotton modules during and after transport to the gin. Mark–release-recapture experiments in August and September in Texas indicated that most weevils disperse rapidly from the module surface, temperature permitting, unless confined under a module tarp, where most died. Nevertheless, 1–5% of released weevils were recovered alive after 24 h on the side and top surfaces of modules, representing potential dispersants. Mortality of boll weevils caged on the top surface of a module was 95–100% after ...


A Global Assembly Of Cotton Ests, Joshua A. Udall, Jordan M. Swanson, Karl Haller, Ryan A. Rapp, Michael Edward Sparks, Jamie Hatfield, Yeisoo Yu, Yingru Wu, Catriona Dowd, Aladdin B. Arpat, Brad A. Sickler, Thea A. Wilkins, Yin Ying Guo, Xiao Ya Chen, Jodi Scheffler, Earl Taliercio, Ricky Turley, Helen Mcfadden, Paxton Payton, Natalya Klueva, Randell Allen, Deshui Zhang, Candace Haigler, Curtis Wilkerson, Jinfeng Suo, Stefan R. Schulze, Margaret L. Pierce, Margaret Essenberg, Hyeran Kim, Danny J. Llewellyn, Elizabeth S. Dennis, Rod Wing, Andrew H. Paterson, Cari Soderlund, Jonathan F. Wendel Feb 2006

A Global Assembly Of Cotton Ests, Joshua A. Udall, Jordan M. Swanson, Karl Haller, Ryan A. Rapp, Michael Edward Sparks, Jamie Hatfield, Yeisoo Yu, Yingru Wu, Catriona Dowd, Aladdin B. Arpat, Brad A. Sickler, Thea A. Wilkins, Yin Ying Guo, Xiao Ya Chen, Jodi Scheffler, Earl Taliercio, Ricky Turley, Helen Mcfadden, Paxton Payton, Natalya Klueva, Randell Allen, Deshui Zhang, Candace Haigler, Curtis Wilkerson, Jinfeng Suo, Stefan R. Schulze, Margaret L. Pierce, Margaret Essenberg, Hyeran Kim, Danny J. Llewellyn, Elizabeth S. Dennis, Rod Wing, Andrew H. Paterson, Cari Soderlund, Jonathan F. Wendel

Ecology, Evolution and Organismal Biology Publications

Approximately 185,000 Gossypium EST sequences comprising >94,800,000 nucleotides were amassed from 30 cDNA libraries constructed from a variety of tissues and organs under a range of conditions, including drought stress and pathogen challenges. These libraries were derived from allopolyploid cotton (Gossypium hirsutum; AT and DT genomes) as well as its two diploid progenitors,Gossypium arboreum (A genome) and Gossypium raimondii (D genome). ESTs were assembled using the Program for Assembling and Viewing ESTs (PAVE), resulting in 22,030 contigs and 29,077 singletons (51,107 unigenes). Further comparisons among the singletons and contigs led to ...


The Pap Operon Of Avian Pathogenic Escherichia Coli Strain O1:K1 Is Located On A Novel Pathogenicity Island, Subhashinie Kariyawasam, Timothy J. Johnson, Lisa K. Nolan Jan 2006

The Pap Operon Of Avian Pathogenic Escherichia Coli Strain O1:K1 Is Located On A Novel Pathogenicity Island, Subhashinie Kariyawasam, Timothy J. Johnson, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

We have identified a 56-kb pathogenicity island (PAI) in avian pathogenicEscherichia coli strain O1:K1 (APEC-O1). This PAI, termed PAI IAPEC-O1, is integrated adjacent to the 3′ end of the pheV tRNA gene. It carries putative virulence genes of APEC (pap operon), other E. coli genes (tia and ireA), and a 1.5-kb region unique to APEC-O1. The kps gene cluster required for the biosynthesis of polysialic acid capsule was mapped to a location immediately downstream of this PAI.


Dna Sequence Of A Colv Plasmid And Prevalence Of Selected Plasmid-Encoded Virulence Genes Among Avian Escherichia Coli Strains, Timothy J. Johnson, Kylie E. Siek, Sara J. Johnson, Lisa K. Nolan Jan 2006

Dna Sequence Of A Colv Plasmid And Prevalence Of Selected Plasmid-Encoded Virulence Genes Among Avian Escherichia Coli Strains, Timothy J. Johnson, Kylie E. Siek, Sara J. Johnson, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

olV plasmids have long been associated with the virulence of Escherichia coli, despite the fact that their namesake trait, ColV production, does not appear to contribute to virulence. Such plasmids or their associated sequences appear to be quite common among avian pathogenic E. coli (APEC) and are strongly linked to the virulence of these organisms. In the present study, a 180-kb ColV plasmid was sequenced and analyzed. This plasmid, pAPEC-O2-ColV, possesses a 93-kb region containing several putative virulence traits, including iss, tsh, and four putative iron acquisition and transport systems. The iron acquisition and transport systems include those encoding aerobactin ...