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Full-Text Articles in Life Sciences

Damaging Capacity Of Five Metal Cations On Escherichia Coli Plasmid Dna, Sara Jo O’Connell Apr 2006

Damaging Capacity Of Five Metal Cations On Escherichia Coli Plasmid Dna, Sara Jo O’Connell

Life and Environmental Sciences Undergraduate Theses

The toxic effects of five metal cations, frequently in contact with humans, have been analyzed in this study. Anthropogenic activities, such as mining, cause elevated levels of cobalt in soil and water. Other common environmental pollutants found in mining effluent include the heavy metals: copper, iron, and manganese. Stannous chloride (SnCl2) is widely used in nuclear medicine as a reducing agent for products radiolabeled with technetium-99m. The ability of these five heavy metals to mediate a Fenton-like reaction was observed through the alteration of plasmid topology. Treatment of Escherichia coli plasmid DNA with CuCl, SnCl2, and FeCl2 resulted in DNA ...


The Minimum Letter Flip Problem For Haplotyping A Single Individual, John Louie Apr 2006

The Minimum Letter Flip Problem For Haplotyping A Single Individual, John Louie

Mathematics, Engineering and Computer Science Undergraduate Theses

When haplotyping a single individual, DNA is replicated, broken into smaller fragments, and then sequenced by a machine. The minimum letter flip problem is one approach to correcting errors that arise in shotgun sequencing. We refer to the minimum letter flip problem as stated in other texts as CGMLF. CGMLF changes a minimal number of single nucleotide polymorphisms (SNPs) to create a feasible SNP matrix. Since finding partitions that have this property is especially difficult, we relate CGMLF to several 2-median problem formulations. Our major result is that the CGMLF is equivalent to a non-polynomial 2-median problem formulation. We develop ...


Detection Of Sporadic Genetic Mutations Using An Endov/Ligase Scanning Assay, Jillian Bybee Apr 2006

Detection Of Sporadic Genetic Mutations Using An Endov/Ligase Scanning Assay, Jillian Bybee

Life and Environmental Sciences Undergraduate Theses

The identification of sporadic point mutations in tumor-suppressor genes, which are responsible for regulating cell growth, has been linked to the early detection of cancer, as well as with identifying those individuals with a genetic predisposition to developing cancer. My research investigates the use of a single-step endonuclease V/ligase scanning assay in the detection of unknown point mutations in genomic DNA. Specifically, the research focuses on the K-Ras gene, which has been linked to several different types of cancer, including colorectal cancer. A universal PCR strategy using fluorescently labeled universal primers and unlabeled gene specific primers allowed for amplification ...