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2004

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Retrospective Theses and Dissertations

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Full-Text Articles in Life Sciences

A Statistical Regression Model To Determine Financial Cash Flow For Wind Energy Based On Tax Structure, Matthew Dae Joong Ritsema Jan 2004

A Statistical Regression Model To Determine Financial Cash Flow For Wind Energy Based On Tax Structure, Matthew Dae Joong Ritsema

Retrospective Theses and Dissertations

A financial cash flow for wind turbines is based on an energy entity's tax structure. Using statistical regression of various wind turbines and wind maps, a theoretical model for energy output was calculated. The turbines were separated by the rated size of the turbine. With the theoretical model, financial output was generated for four different types of tax structures. A total of eight test cases were simulated. Special consideration was given to the impact of Production Tax Credits (PTC) and Renewable Energy Production Incentives (REPI). The object is to determine what type of business and tax structure would have ...


Fertility In Angus Females , Jennifer Arleen Minick Jan 2004

Fertility In Angus Females , Jennifer Arleen Minick

Retrospective Theses and Dissertations

Reproduction is economically important to the beef producer. The objective of this study was to determine if pregnancy rate, calving day (CD) and age at first calving (AFC) could be used to select for female fertility in Angus beef cattle. Producers from six herds in five states provided 3144 heifer records. A general linear animal model, using the relationship matrix, was fitted to the binary trait of pregnancy status by the software Matvec. The heritability of pregnancy percentage on the underlying scale was 0.13 +/- 0.07. Estimated breeding values, which are in units of underlying fertility, ranged from -0 ...


The Identification And Characterization Of P5 And P2 Colonization Proteins In Haemophilus Parasuis And The Targeted Binding Of Carcinoembryonic Antigen (Cea) , Jerry Kim Mcvicker Jan 2004

The Identification And Characterization Of P5 And P2 Colonization Proteins In Haemophilus Parasuis And The Targeted Binding Of Carcinoembryonic Antigen (Cea) , Jerry Kim Mcvicker

Retrospective Theses and Dissertations

The outer membrane protein P5 from Haemophilus influenzae has been shown to aid colonization in the human nasopharynx by binding to CD66 (CEA) on the surface of mucosal epithelial cells. The intent here was to determine if Haemophilus parasuis contained homologs of the P5 and P2 proteins and to characterize these proteins by SDS-PAGE, IEF, and CEA binding. Using outer membrane protein P5 primers designed from the H. influenzae database, we were able to detect a single PCR product in a H. parasuis field isolate. The PCR product was transformed into a competent E. coli strain and sequenced. DNA sequence ...


The Identification, Cloning, And Characterization Of A Phospholipase C-Beta Isoform From The Sea Urchin Egg , Andre Peter Joseph Kulisz Jan 2004

The Identification, Cloning, And Characterization Of A Phospholipase C-Beta Isoform From The Sea Urchin Egg , Andre Peter Joseph Kulisz

Retrospective Theses and Dissertations

This work was the first to provide direct evidence for the presence of a PLCbeta isoform within the sea urchin egg, named suPLCbeta. The coding sequence was identified and cloned from the eggs of Lytechinus pictus and when translated, determined to have the greatest degree of homology and identity with the mammalian PLCbeta4 isoforms. This alone is a unique finding in that the presence of PLCbeta4 has only been found in the retina and specific neuronal tissues. The presence of the protein within the egg was verified using antibodies that were generated specifically against suPLCbeta, which indicate that the majority ...


Confounding Factors And Tertiary-Phase Control By A Surfactive Agent On Smectite Sorption Of Atrazine , Mark Albert Chappell Jan 2004

Confounding Factors And Tertiary-Phase Control By A Surfactive Agent On Smectite Sorption Of Atrazine , Mark Albert Chappell

Retrospective Theses and Dissertations

Atrazine is a widely used commercial pesticide applied to soil for the control broadleaf weeds in agriculture. Frequent detection of atrazine in surface and subsurface waters and the potential for adverse effects on toxicity to aquatic and terrestrial life have generated numerous investigations into the mechanisms governing atrazine sorption by soils. This dissertation seeks to add to that body of knowledge by elucidating basic mechanisms and processes that influence sorption of atrazine on clays in the presence and absence of surfactants commonly used in commercial formulations. Sorption studies showed that air-dried (AD), resuspended K+-saturated PC (KPC) exhibited a significantly ...


Quantitative Assessment Of Kernel Set And Risk Of Out-Crossing In Maize Based On Flowering Dynamics , Agustin Ezequiel Fonseca Jan 2004

Quantitative Assessment Of Kernel Set And Risk Of Out-Crossing In Maize Based On Flowering Dynamics , Agustin Ezequiel Fonseca

Retrospective Theses and Dissertations

Pollination in maize can occur only if airborne pollen shed by the staminate flowers on the tassel is captured by the stigmas of the pistillate flowers borne on the ear. Since maize domestication, and especially with the introduction of hybrid seed production, many attempts have been made to control pollination to ensure maximum kernel set and high levels of genetic purity. The central hypothesis of this dissertation is that pollen shed and silk emergence are predictable processes, which can be simply quantified and used to characterize the capacity of the male inbreds to produce pollen and the ability of the ...


Protein-Protein Interaction And Electron Transfer: Case Of Cytochrome F And Cytochrome C6 , Tijana Žarković Grove Jan 2004

Protein-Protein Interaction And Electron Transfer: Case Of Cytochrome F And Cytochrome C6 , Tijana Žarković Grove

Retrospective Theses and Dissertations

We used photo-induced reaction as a tool to look to explore dynamics of the protein-protein interaction between cytochrome f and cytochrome c6 from C. reinhardtii. In our studies of electron transfer between 3Zncyt c6 and cyt f(III) we discovered two distinct intracomplex processes corresponding to electron transfer within diprotein complexes termed persistent and transient. The overall reaction remained biphasic and the rate constants for both processes were invariant to the change of ionic strength within the studied interval. The docking of these proteins is due to strong hydrophobic interaction slightly augmented by weak electrostatic attraction. We used covalent cross-linking ...


Pathogenesis And Transmission Of Hepatitis E Virus (Hev) In Pigs, Chaiyan Kasorndorkbua Jan 2004

Pathogenesis And Transmission Of Hepatitis E Virus (Hev) In Pigs, Chaiyan Kasorndorkbua

Retrospective Theses and Dissertations

Human hepatitis E virus (HEV) was first reported in 1980 and is now considered a major cause of acute non-A, non-B hepatitis in humans. HEV infection is reported throughout the world and occurs in epidemic form in developing countries where the disease is endemic and is often associated with water contamination after heavy rains or flooding. Sporadic human HEV infections occur in industrialized countries where infected individuals contract the infection while traveling to endemic regions. The fecal-oral route is considered the primary mode of HEV transmission in humans. HEV infection of pigs was discovered in 1997. Since 1997, sporadic HEV ...


Effect Of Randomization On The Oxidative Stability Of Corn Oil , Yongzhi Jiang Jan 2004

Effect Of Randomization On The Oxidative Stability Of Corn Oil , Yongzhi Jiang

Retrospective Theses and Dissertations

The oxidative stability of natural and randomized corn oil was studied at high temperature (80 or 100°C) by using an OSI (oxidative stability instrument) and at low temperature (28, 40 or 50°C) by using peroxide values determined by the ferrous iron oxidation test. Randomized corn oil without purification oxidized 1 to 5 times faster than did natural corn oil, depending on the particular lot of corn oil. After alumina-column purification, which removed the tocopherols and other polar compounds, there was no difference in the oxidation rates of the randomized and natural corn oil. These findings suggest that alteration ...


The Central Role Of Acetyl-Coa In Plant Metabolism, As Examined Through Studies Of Atp Citrate Lyase And The Bio1 Mutant Of Arabidopsis , Elizabeth K. Winters Jan 2004

The Central Role Of Acetyl-Coa In Plant Metabolism, As Examined Through Studies Of Atp Citrate Lyase And The Bio1 Mutant Of Arabidopsis , Elizabeth K. Winters

Retrospective Theses and Dissertations

The acetyl-CoA/biotin network encompasses acetyl-CoA generation and utilization, biotin synthesis, recovery, and transport, and myriad metabolic processes requiring biotin-containing enzymes. To expand understanding of this network and its control, plants with altered levels of ACL (ATP citrate lyase) and BIO1 (adenosylmethionine-8-amino-7-oxononanoate synthase) were examined.;An inducible promoter provides a powerful method to test the effects of altered expression of specific genes. The synthetic GVG promoter, reported to be sterol-inducible in Arabidopsis thaliana, was inducible in Glycine max (soybean) when used to control the GUS reporter gene, or the ACLA or ACLB genes. It showed the same unique pattern in ...


Molecular And Functional Analysis Of Drosophila Hillarin And Its Leech Homolog , Yun Ji Jan 2004

Molecular And Functional Analysis Of Drosophila Hillarin And Its Leech Homolog , Yun Ji

Retrospective Theses and Dissertations

This thesis describes the molecular and functional analysis of the leech Hillarin and its Drosophila homolog D-hil. Leech Hillarin was identified by screening a leech expression library with the mAb Lan3-15, which specifically labels the axon hillock region of leech neurons. Database searches revealed that leech Hillarin has a number of potential homologs in a variety of organisms such as yeast, cyanobacterium, fly, nematode, mouse and human and that these proteins share two novel protein domains, the W180 domain and the H domain. The W180 domain is enriched with tryptophans whereas the H domain shares high number of invariant residues ...


Molecular Mechanism Of Imprinting Of The Maize Endosperm Gene Fie1 , Pedro Hermon-Cruz Jan 2004

Molecular Mechanism Of Imprinting Of The Maize Endosperm Gene Fie1 , Pedro Hermon-Cruz

Retrospective Theses and Dissertations

The main purpose of this study was to characterize the expression patterns of the maize fie genes and to identify possible modes of their regulation. Our study revealed that although the fie1 and fie2 genes in maize contain a 78% homology within their exonic regions, their promoter regions are complex and contain no similarity. The maize fie1 and fie2 genes also have different pattern of expression. fie1 has a highly regulated expression. fie1 transcription is detectable only in the endosperm at early stages of development. fie2 has a more ubiquitous expression, detectable in vegetative and reproductive tissues at all times ...


Host Specialization, Intersterility, And Taxonomy Of Populations Of Ceratocystis Fimbriata From Sweet Potato, Sycamore, And Cacao , Christine Jeanette Baker Engelbrecht Jan 2004

Host Specialization, Intersterility, And Taxonomy Of Populations Of Ceratocystis Fimbriata From Sweet Potato, Sycamore, And Cacao , Christine Jeanette Baker Engelbrecht

Retrospective Theses and Dissertations

Ceratocystis fimbriata is a large complex of fungal species that cause wilt-type diseases on a variety of temperate and tropical plants. Within the Latin American clade of C. fimbriata, isolates from sweet potato (Ipomoea batatas), sycamore ( Platanus spp.), and cacao (Theobroma cacao) form monophyletic lineages and are specialized to these three hosts. The sweet potato and sycamore lineages correspond to intersterility groups, and there are two intersterility groups within the cacao lineage, corresponding to a sublineage in western Ecuador and a second sublineage in Brazil, Colombia, and Costa Rica. The cacao lineage is described as a new species, C. cacaofunesta ...


Quality Characteristics Of Irradiated Ready-To-Eat Meats , Terry Andrew Houser Jan 2004

Quality Characteristics Of Irradiated Ready-To-Eat Meats , Terry Andrew Houser

Retrospective Theses and Dissertations

Sliced, ham and all-pork frankfurters were irradiated at 1.6 kGy and evaluated for color, lipid oxidation, odor, flavor, and the production of volatiles over an 8-week storage period. Irradiation processing did not affect color or lipid oxidation values for the ham or frankfurters. Irradiation processing increased off-odor scores for the ham but not for frankfurters. Off-flavor scores were not significantly different for ham but were higher in frankfurters due to irradiation processing. Dimethyl disulfide content increased as a result of irradiation in both products but decreased over the 8-week storage period.;Corned beef, roast beef, all-beef frankfurters, chicken roll ...


Effects Of [Beta]-Conglycinin, Soy Isoflavones, And Group B Soyasaponins On Plasma Lipid Concentrations , Sun-Ok Lee Jan 2004

Effects Of [Beta]-Conglycinin, Soy Isoflavones, And Group B Soyasaponins On Plasma Lipid Concentrations , Sun-Ok Lee

Retrospective Theses and Dissertations

The effects on plasma lipid concentrations of beta-conglycinin, one of the two major storage protei (213 mumol v. 22 mumol/day) and soyasaponins (2176 mumol v. 23 mumol/day) and the mechanism of beta-conglycinin to affect plasma lipid levels were investigated in mildly hypercholesterolemic women. A significant reduction of plasma total and LDL cholesterol occured after 14 and 28 days during ingestion of beta-conglycinin but only when it contained high levels of isoflavones and soyasaponins. A slight but nonsignificant increase in excretion of fecal bile acids and neutral sterols may have contributed to these changes. This study suggests that isoflavones ...


Investigation Of Genetic Susceptibility To The Porcine Reproductive And Respiratory Syndrome Virus Between Commercial Breeding Stock Lines Of Pigs , Amy Louise Vincent Jan 2004

Investigation Of Genetic Susceptibility To The Porcine Reproductive And Respiratory Syndrome Virus Between Commercial Breeding Stock Lines Of Pigs , Amy Louise Vincent

Retrospective Theses and Dissertations

The objectives in the studies described herein were to evaluate the use of an antemortem in vitro test as an indicator in identifying commercial breeding stock lines with high and low susceptibility to PRRSV and to evaluate the pigs from lines selected from the first objective in an experimental infection model. Macrophages have been shown to be the cell type PRRSV preferentially infects and subsequently manipulates to achieve efficient replication and production of progeny virions. The in vitro assay was therefore designed to evaluate the percentage of macrophages derived from peripheral blood monocytes that are susceptible to infection in culture ...


Investigation Of Microcin 24 For Applications In Food Safety, Mechanism Of Resistance And Effect On Activity After Site-Directed Mutagenesis , Timothy Steven Frana Jan 2004

Investigation Of Microcin 24 For Applications In Food Safety, Mechanism Of Resistance And Effect On Activity After Site-Directed Mutagenesis , Timothy Steven Frana

Retrospective Theses and Dissertations

Microcin 24 is an antimicrobial peptide produced by Escherichia coli with activity against Salmonella typhimurium. In this study microcin 24 was investigated for its potential use in food safety, resistance development, and structure-activity relationship following site-directed mutagenesis. An attempt was made to produce microcin 24 from lactic acid bacteria by substituting the natural, dedicated leader peptide microcin-producing gene with a signal leader from divergicin A, which accesses the general secretion pathway. RNA sequences could be recovered from Carnobacterium divergens harboring an expression vector with the fusion construct, but no microcin activity could be detected. Concurrently, development and mechanism of resistance ...


An Agent-Based Computational Model Of The Evolution Of Reproductive Isolation In Populations Of Digital Plants , Nicole Patricia Leahy Jan 2004

An Agent-Based Computational Model Of The Evolution Of Reproductive Isolation In Populations Of Digital Plants , Nicole Patricia Leahy

Retrospective Theses and Dissertations

The process of speciation is a fascinating area of research in the biological sciences. Development of mathematical and computational models are an important part of developing speciation theory. The Pseudophyte Evolutionary Algorithm PEA is a individual-based population model developed to test hypotheses concerning process of speciation. The individuals pseudophytes are digital "plants." Biologically, they are haploid annuals, each producing pollen and ovules. Pseudophytes are implemented as binary strings representing loci which encoded six traits. Loci were divided into two chromosomes each with three traits. The loci are mapped to a phenotype which determines the behavior of the pseudophyte. The PEA ...


Characterization Of Rev Variants Selected During Chronic And Inapparent Stages Of Equine Infectious Anemia Virus Infection, Wendy Olissa Sparks Jan 2004

Characterization Of Rev Variants Selected During Chronic And Inapparent Stages Of Equine Infectious Anemia Virus Infection, Wendy Olissa Sparks

Retrospective Theses and Dissertations

Equine infectious anemia virus (EIAV) exhibits a high rate of genetic variation in vivo and results in a clinically variable disease in infected horses. Previous studies identified distinct subpopulations within the Rev quasispecies of an experimentally infected pony, pony 524. The subpopulations showed significantly different phenotypes, as measured by in vitro assays, and fluctuated in dominance in a manner coincident with clinical stage of disease. This study further characterizes the genotypic and phenotypic variation within and between the previously identified EIAV Rev subpopulations in pony 524, and characterizes the EIAV Rev quasispecies of another EIAV-infected pony, pony 625. Within pony ...


Flux Analysis In Central Carbon Metabolism In Plants: 13c Nmr Experiments And Analysis , Ganesh Sriram Jan 2004

Flux Analysis In Central Carbon Metabolism In Plants: 13c Nmr Experiments And Analysis , Ganesh Sriram

Retrospective Theses and Dissertations

Metabolic flux analysis is crucial in metabolic engineering. This research concentrated on improvements in 13C labeling-based flux analysis, a powerful flux quantification method, particularly oriented toward application to plants. Furthermore, systemic 13C flux analyses were performed on two model plant systems: Glycine max (soybean) embryos, and Catharanthus roseus hairy roots.;The concepts 'bond integrity', 'bondomer' and the algorithm 'Boolean function mapping' were introduced, to facilitate efficient flux evaluation from carbon bond labeling experiments, and easier flux identifiability analysis.;13C labeling experiments were performed on developing soybean (Glycine max) embryos and C. roseus hairy roots. A computer program, NMR2Flux, was developed ...


Supply Chain Management Of Differentiated Agricultural Products Under Imperfect Information , Miguel Alberto Carriquiry Jan 2004

Supply Chain Management Of Differentiated Agricultural Products Under Imperfect Information , Miguel Alberto Carriquiry

Retrospective Theses and Dissertations

Participants in a supply chain of agricultural value added products face two significant challenges. First, many of the costly distinctive traits being desired by consumers are difficult (if not impossible) to observe even after consumption. In order for markets for these classes of goods to develop, firms touting the quality of the product need to be trusted. Hence, maintaining an excellent reputation is essential for firms to keep their customers' good will. Secondly, production is conducted in an environment of yield uncertainty, making it impossible for producers and processors to predict with certainty either the quantity or the quality of ...


Panel Econometric Evidence Of Chinese Agricultural Household Behavior In The Later 1990s: Production Efficiency, Size Effects And Human Mobility , Zhuo Chen Jan 2004

Panel Econometric Evidence Of Chinese Agricultural Household Behavior In The Later 1990s: Production Efficiency, Size Effects And Human Mobility , Zhuo Chen

Retrospective Theses and Dissertations

The dissertation consists of three essays that analyze production efficiency, size effects and human mobility using a panel of 591 Chinese agricultural households in the later 1990s.;The first essay estimates technical efficiency through the framework of a translog stochastic production frontier with a behavioral inefficiency component. The results reveal a decreasing trend of output elasticities with respect to labor and fertilizer. The marginal effects of inefficiency terms show significant output gains by eliminating land fragmentation, improving access to education in rural area, and promoting specialization and mechanization.;The second essay examines land heterogeneities and the empirical inverse relationship in ...


Soil And Runoff Phosphorus As Affected By Fertilizer And Manure Application , Brett Lee Allen Jan 2004

Soil And Runoff Phosphorus As Affected By Fertilizer And Manure Application , Brett Lee Allen

Retrospective Theses and Dissertations

Fertilizer or manure applications are increasing soil P in agricultural fields. This study assessed the impact of P application on soil P fractions and P loss with surface runoff based on P rate field trials and both indoor and field rainfall simulations. Bray P, Mehlich-3 (M3) P, Olsen P, total P (TPS) and soil P saturation (Psat) estimated by M3 or oxalate extractable P, Fe, and Al molar ratios increased with cumulative P application (0 to 1098 kg P ha-1 after 4 to 23 yr) at plots from 11 trials. Soil test P, TPS, and Psat were linearly correlated. Routine ...


The God Factor: The Role Of Religious Involvement On Depression, Health, And Well-Being , Arlene Maria De La Mora Jan 2004

The God Factor: The Role Of Religious Involvement On Depression, Health, And Well-Being , Arlene Maria De La Mora

Retrospective Theses and Dissertations

This study was designed to explore the relationships between religious involvement and health and well-being utilizing multiple measures of religious involvement at two time points. At Time 1, participants (N = 184) recruited from three Midwestern universities completed a battery of questionnaires assessing religious beliefs/attitudes participation in public and private religious activities (i.e., frequency of church attendance and prayer), depression, perceived health, and frequency of seeking health care services. At Time 2, participants were again asked to complete questionnaires assessing depression, perceived health and frequency of seeking health care. The effects of religious involvement on depression, perceived health and ...


Modeling Surface Soil And Phosphorus Transport Using Dssat Models , Mohamud Hussein Egeh Jan 2004

Modeling Surface Soil And Phosphorus Transport Using Dssat Models , Mohamud Hussein Egeh

Retrospective Theses and Dissertations

Computer simulation models are regarded as important tools to address concerns about the environmental impact of agricultural activities and have increasingly been used to complement field research. The CERES-Maize and CROPGRO-Soybean, two of the main crop growth models incorporated into the Decision Support System for Agrotechnology Transfer (DSSAT), were developed to simulate the effects of environmental conditions, different management strategies, and genetics on crop growth and development. These models currently do not have an option for calculating erosion and phosphorus (P) dynamics. In this study, the Modified Universal Soil Loss Equation (MUSLE) and sediment-bound P model were incorporated into CERES-Maize ...


Using Surface Solute Transport Properties Measured By Time Domain Reflectometry To Predict Subsurface Leaching , Anju Gaur Jan 2004

Using Surface Solute Transport Properties Measured By Time Domain Reflectometry To Predict Subsurface Leaching , Anju Gaur

Retrospective Theses and Dissertations

Solute transport properties are required to evaluate the risk of contaminating ground water with agricultural chemicals under a wide variety of crop and soil management practices. Most solute transport measurement techniques are tedious and lead to extensive soil excavation. Two experiments were performed to evaluate whether surface transport properties determined by a non-destructive time domain reflectometry (TDR) technique could be used to accurately predict subsurface leaching. TDR probes installed in the surface 2-cm of soil were used to determine resident solute concentration from measured soil surface soil bulk electrical conductivity. Resident concentrations were analyzed with a one-dimensional (1-D) solute transport ...


Molecular, Bioinformatic And Statistical Approaches To Identify Genes Underlying Complex Traits In Livestock , Laura Grapes Jan 2004

Molecular, Bioinformatic And Statistical Approaches To Identify Genes Underlying Complex Traits In Livestock , Laura Grapes

Retrospective Theses and Dissertations

One of the primary goals for molecular geneticists working with livestock species is to identify and characterize genes underlying complex traits, the so-called quantitative trait loci (QTL). The primary strategy for identifying QTL involves several steps, one being fine mapping of a previously defined chromosomal region and another being identification of candidate genetic polymorphisms that may cause differences in phenotype. The studies presented in this dissertation address fine mapping methodology, use of the candidate gene approach for directly identifying candidate genetic polymorphisms and use of bioinformatic tools for identifying genetic polymorphisms in silico. Results from simulation studies suggest that two ...


New Palladium-Catalyzed Approaches To Heterocycles And Carbocycles, Qinhua Huang Jan 2004

New Palladium-Catalyzed Approaches To Heterocycles And Carbocycles, Qinhua Huang

Retrospective Theses and Dissertations

The tert-butylimines of o-(1-alkynyl)benzaldehydes and analogous pyridinecarbaldehydes have been cyclized under very mild reaction conditions in the presence of I2, ICl, PhSeCl, PhSCl and p-O2NC6H4SCl to give the corresponding halogen-, selenium- and sulfur-containing disubstituted isoquinolines and naphthyridines, respectively. Monosubstituted isoquinolines and naphthyridines have been synthesized by the metal-catalyzed ring closure of these same iminoalkynes. This methodology accommodates a variety of iminoalkynes and affords the anticipated heterocycles in moderate to excellent yields.;The Pd(II)-catalyzed cyclization of 2-(1-alkynyl)arylaldimines in the presence of various alkenes provides an efficient way to synthesize a variety of 4-(1-alkenyl)-3-arylisoquinolines ...


The Influence Of Cultural Practice, Biology And Environment On Maize Pollen Travel , Dale Steven Ireland Jan 2004

The Influence Of Cultural Practice, Biology And Environment On Maize Pollen Travel , Dale Steven Ireland

Retrospective Theses and Dissertations

Genetically modified agricultural field crops have provided a new lens with which to view transgenic plant engineering. Production of hybrid corn (Zea mays L) depends on cross-pollination between male and female inbred parents. As such, reproductive isolation of seed fields is required to ensure genetic purity of the hybrid progeny. Customer demand for improved genetic purity prompted the seed industry to examine the level of genetic purity resulting from current isolation practices. This project was a two-pronged approach: (1) A three-year study was conducted to monitor purity of hybrid seed produced in 291 fields from 24 seed companies in North ...


Expression Analysis Of Maize Opaque2 Mutants And Glu-1dx5 Transgenic Corn , Hongwu Jia Jan 2004

Expression Analysis Of Maize Opaque2 Mutants And Glu-1dx5 Transgenic Corn , Hongwu Jia

Retrospective Theses and Dissertations

The maize (Zea mays L.) opaque2 (o2) mutant has high lysine content, but soft and starchy kernels. We confirm that Lys content, kernel density, and reduction of zein proteins in o2 mutants are genetic-background dependent. Microarray analysis was carried out by pooling the same amount of mRNA from eight o2 lines to probe the maize Unigene cDNA chip to understand the o2 main effects. 202 genes identified as differentially expressed fit our definition of main effect genes. Comparative analysis of the expression profiles of two additional inbred lines shows that B46WT/B46 o2 with the most extreme phenotype has the ...