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Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

2004

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University of Nebraska - Lincoln

Agronomy and Crop Sciences

Apigeninidin; Cochliobolus heterostrophus; Cytochrome P450; Eriodictyol; Flavonoid 30-hydroxylase; Luteolinidin; Naringenin; Phytoalexin; Sorghum bicolor; 3-Deoxyanthocyanidins

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Full-Text Articles in Life Sciences

Expression Of A Putative Flavonoid 3'-Hydroxylase In Sorghum Mesocotyls Synthesizing 3-Deoxyanthocyanidin Phytoalexins, Jayanand Boddu, Catherine Svabek, Rajandeep Sekhon, Amanda Gevens, Ralph L. Nicholson, A. Daniel Jones, Jeffrey F. Pedersen, David L. Gustine, Surinder Chopra Jan 2004

Expression Of A Putative Flavonoid 3'-Hydroxylase In Sorghum Mesocotyls Synthesizing 3-Deoxyanthocyanidin Phytoalexins, Jayanand Boddu, Catherine Svabek, Rajandeep Sekhon, Amanda Gevens, Ralph L. Nicholson, A. Daniel Jones, Jeffrey F. Pedersen, David L. Gustine, Surinder Chopra

Agronomy & Horticulture -- Faculty Publications

In sorghum, ingress of Cochliobolus heterostrophus stimulates the synthesis of 3-deoxyanthocyanidins that act as phytoalexins. Apigeninidin and luteolinidin are two major phytoalexins induced in the first 24 h after infection. In an attempt to understand genetic regulation of the biosynthesis of sorghum phytoalexins, we isolated a differentially expressed partial cDNA. Characterization and comparison showed that this cDNA sequence corresponds to a putative flavonoid 3-hydroxylase. Full length sequence characterization allowed us to establish that the sorghum putative f3h cDNA encodes a peptide of 517 amino acids that has domains conserved among cytochrome P450 proteins functioning in the flavonoid biosynthetic ...