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Full-Text Articles in Life Sciences

Lhx9 And Lhx9alpha: Differential Biochemical Properties And Effects On Neuronal Differentiation, Bertrand Molle, Stephane Pere, Vieri Failli, Ingolf Bach, Sylvie Retaux Dec 2004

Lhx9 And Lhx9alpha: Differential Biochemical Properties And Effects On Neuronal Differentiation, Bertrand Molle, Stephane Pere, Vieri Failli, Ingolf Bach, Sylvie Retaux

Program in Gene Function and Expression Publications and Presentations

The Lhx9 LIM-homeodomain transcription factor and its truncated isoform Lhx9alpha are generated by alternative splicing of the Lhx9 gene. Here we investigated the differential functional properties of these two isoforms. Lhx9alpha, which lacks parts of the homeodomain, was unable to bind DNA in EMSA experiments, but was able to associate with CLIM cofactors in GST pull-down assays. In transfection experiments in PC12 cells, Lhx9alpha fusion constructs systematically showed a nuclear localization, as opposed to Lhx9 fusion constructs, which also localized to the cytoplasm. Moreover, Lhx9 increased NGF-induced neuronal differentiation of PC12 cells. Lhx9alpha, on the other hand, did not significantly ...


A First Version Of The Caenorhabditis Elegans Promoterome, Denis Dupuy, Qian-Ru Li, Bart Deplancke, Mike Boxem, Tong Hao, Philippe Lamesch, Reynaldo Sequerra, Stephanie Bosak, Lynn Doucette-Stamm, Ian A. Hope, David E. Hill, Albertha J. M. Walhout, Marc Vidal Oct 2004

A First Version Of The Caenorhabditis Elegans Promoterome, Denis Dupuy, Qian-Ru Li, Bart Deplancke, Mike Boxem, Tong Hao, Philippe Lamesch, Reynaldo Sequerra, Stephanie Bosak, Lynn Doucette-Stamm, Ian A. Hope, David E. Hill, Albertha J. M. Walhout, Marc Vidal

Program in Gene Function and Expression Publications and Presentations

An important aspect of the development of systems biology approaches in metazoans is the characterization of expression patterns of nearly all genes predicted from genome sequences. Such "localizome" maps should provide information on where (in what cells or tissues) and when (at what stage of development or under what conditions) genes are expressed. They should also indicate in what cellular compartments the corresponding proteins are localized. Caenorhabditis elegans is particularly suited for the development of a localizome map since all its 959 adult somatic cells can be visualized by microscopy, and its cell lineage has been completely described. Here we ...


Regulation Of Muscle Fiber Type And Running Endurance By Ppardelta, Yong-Xu Wang, Chung-Li Zhang, Ruth T. Yu, Helen K. Cho, Michael C. Nelson, Corinne R. Bayuga-Ocampo, Jungyeob Ham, Heonjoong Kang, Ronald M. Evans Aug 2004

Regulation Of Muscle Fiber Type And Running Endurance By Ppardelta, Yong-Xu Wang, Chung-Li Zhang, Ruth T. Yu, Helen K. Cho, Michael C. Nelson, Corinne R. Bayuga-Ocampo, Jungyeob Ham, Heonjoong Kang, Ronald M. Evans

Program in Gene Function and Expression Publications and Presentations

Endurance exercise training can promote an adaptive muscle fiber transformation and an increase of mitochondrial biogenesis by triggering scripted changes in gene expression. However, no transcription factor has yet been identified that can direct this process. We describe the engineering of a mouse capable of continuous running of up to twice the distance of a wild-type littermate. This was achieved by targeted expression of an activated form of peroxisome proliferator-activated receptor delta (PPARdelta) in skeletal muscle, which induces a switch to form increased numbers of type I muscle fibers. Treatment of wild-type mice with PPARdelta agonist elicits a similar type ...


A Mechanical Basis For Chromosome Function, Nancy Kleckner, Denise Zickler, Gareth H. Jones, Job Dekker, Ruth Padmore, Jim Henle, John N. Hutchinson Aug 2004

A Mechanical Basis For Chromosome Function, Nancy Kleckner, Denise Zickler, Gareth H. Jones, Job Dekker, Ruth Padmore, Jim Henle, John N. Hutchinson

Program in Gene Function and Expression Publications and Presentations

We propose that chromosome function is governed by internal mechanical forces generated by programmed tendencies for expansion of the DNA/chromatin fiber against constraining features.


Compartment-Specific Perturbation Of Protein Handling Activates Genes Encoding Mitochondrial Chaperones, Takunari Yoneda, Cristina Benedetti, Fumihiko Urano, Scott G. Clark, Heather P. Harding, David Ron Jul 2004

Compartment-Specific Perturbation Of Protein Handling Activates Genes Encoding Mitochondrial Chaperones, Takunari Yoneda, Cristina Benedetti, Fumihiko Urano, Scott G. Clark, Heather P. Harding, David Ron

Program in Gene Function and Expression Publications and Presentations

Protein folding in the mitochondria is assisted by nuclear-encoded compartment-specific chaperones but regulation of the expression of their encoding genes is poorly understood. We found that the mitochondrial matrix HSP70 and HSP60 chaperones, encoded by the Caenorhabditis elegans hsp-6 and hsp-60 genes, were selectively activated by perturbations that impair assembly of multi-subunit mitochondrial complexes or by RNAi of genes encoding mitochondrial chaperones or proteases, which lead to defective protein folding and processing in the organelle. hsp-6 and hsp-60 induction was specific to perturbed mitochondrial protein handling, as neither heat-shock nor endoplasmic reticulum stress nor manipulations that impair mitochondrial steps in ...