Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Overexpression Of Glycine-Extended Gastrin Inhibits Parietal Cell Loss And Atrophy In The Mouse Stomach, Guanglin Cui, Theodore J. Koh, Duan Chen, Chun-Mei Zhao, Shigeo Takaishi, Graham J. Dockray, Andrea Varro, Arlin B. Rogers, James G. Fox, Timothy C. Wang Nov 2004

Overexpression Of Glycine-Extended Gastrin Inhibits Parietal Cell Loss And Atrophy In The Mouse Stomach, Guanglin Cui, Theodore J. Koh, Duan Chen, Chun-Mei Zhao, Shigeo Takaishi, Graham J. Dockray, Andrea Varro, Arlin B. Rogers, James G. Fox, Timothy C. Wang

Open Access Articles

Recently we have reported synergistic effects between glycine-extended gastrin (G-gly) and amidated gastrin-17 on acid secretion in short-term infusion studies. In the present study, we examined the long-term effect of G-gly on the atrophy-promoting effects of amidated gastrin in the mouse stomach with or without Helicobacter infection. Transgenic mice overexpressing amidated gastrin (INS-GAS mice), G-gly (MTI/G-gly mice), and both peptides (INS-GAS/G-gly mice) were used for assessment of acid secretion and ulcer susceptibility and histologic examination and scoring of preneoplastic lesions in response to the 3 and 6 months Helicobacter felis (H. felis) infection. We found that MTI/G-gly ...


The C-Terminus Of Rat L-Histidine Decarboxylase Specifically Inhibits Enzymic Activity And Disrupts Pyridoxal Phosphate-Dependent Interactions With L-Histidine Substrate Analogues, John V. Fleming, Ignacio Fajardo, Michael R. Langlois, Francisca Sanchez-Jimenez, Timothy C. Wang Apr 2004

The C-Terminus Of Rat L-Histidine Decarboxylase Specifically Inhibits Enzymic Activity And Disrupts Pyridoxal Phosphate-Dependent Interactions With L-Histidine Substrate Analogues, John V. Fleming, Ignacio Fajardo, Michael R. Langlois, Francisca Sanchez-Jimenez, Timothy C. Wang

Open Access Articles

Full-length rat HDC (L-histidine decarboxylase) translated in reticulocyte cell lysate reactions is inactive, whereas C-terminally truncated isoforms are capable of histamine biosynthesis. C-terminal processing of the approximately 74 kDa full-length protein occurs naturally in vivo, with the production of multiple truncated isoforms. The minimal C-terminal truncation required for the acquisition of catalytic competence has yet to be defined, however, and it remains unclear as to why truncation is needed. Here we show that approximately 74 kDa HDC monomers can form dimers, which is the conformation in which the enzyme is thought to be catalytically active. Nevertheless, the resulting dimer is ...