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2004

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University of Massachusetts Amherst

Kelly Nevin

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

In Situ Expression Of Nigd In Geobacteraceae In Subsurface Sediments, Dawn E. Holmes, Kelly P. Nevin, Derek Lovley Dec 2004

In Situ Expression Of Nigd In Geobacteraceae In Subsurface Sediments, Dawn E. Holmes, Kelly P. Nevin, Derek Lovley

Kelly Nevin

In order to determine whether the metabolic state of Geobacteraceae involved in bioremediation of subsurface sediments might be inferred from levels of mRNA for key genes, in situ expression of nifD, a highly conserved gene involved in nitrogen fixation, was investigated. When Geobacter sulfurreducens was grown without a source of fixed nitrogen in chemostats with acetate provided as the limiting electron donor and Fe(III) as the electron acceptor, levels of nifD transcripts were 4 to 5 orders of magnitude higher than in chemostat cultures provided with ammonium. In contrast, the number of transcripts of recA and the 16S rRNA ...


Isolation, Characterization, And U(Vi)-Reducing Potential Of A Facultatively Anaerobic, Acid-Resistant Bacterium From Low-Ph, Nitrate- And U(Vi) Contaminated Subsurface Sediment And Description Of Salmonella Subterranea Sp.Nov., Evgenya S. Shelobolina, Sara A. Sullivan, Kathleen R. O'Neil, Kelly P. Nevin, Derek Lovley May 2004

Isolation, Characterization, And U(Vi)-Reducing Potential Of A Facultatively Anaerobic, Acid-Resistant Bacterium From Low-Ph, Nitrate- And U(Vi) Contaminated Subsurface Sediment And Description Of Salmonella Subterranea Sp.Nov., Evgenya S. Shelobolina, Sara A. Sullivan, Kathleen R. O'Neil, Kelly P. Nevin, Derek Lovley

Kelly Nevin

A facultatively anaerobic, acid-resistant bacterium, designated strain FRCl, was isolated from a low-pH, nitrate- and U(VI)-contaminated subsurface sediment at site FW-024 at the Natural and Accelerated Bioremediation Research Field Research Center in Oak Ridge, Tenn. Strain FRCl was enriched at pH 4.5 in minimal medium with nitrate as the electron acceptor, hydrogen as the electron donor, and acetate as the carbon source. Clones with 16S ribosomal DNA (rDNA) sequences identical to the sequence of strain FRCl were also detected in a U(VI)-reducing enrichment culture derived from the same sediment. Cells of strain FRCl were gram-negative ...