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Life Sciences Commons

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2004

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Medicine and Health Sciences

Veterinary microbiology and preventive medicine

Articles 1 - 5 of 5

Full-Text Articles in Life Sciences

Investigation Of Immune Response And Protection Induced By A Combined Vaccination Of Modified Live And Killed Porcine Reproductive And Respiratory Syndrome Virus Vaccines And Its Potential Use In A Swine Herd , Dachrit Nilubol Jan 2004

Investigation Of Immune Response And Protection Induced By A Combined Vaccination Of Modified Live And Killed Porcine Reproductive And Respiratory Syndrome Virus Vaccines And Its Potential Use In A Swine Herd , Dachrit Nilubol

Retrospective Theses and Dissertations

Commercial modified live (MLV) and killed (KV) porcine reproductive and respiratory syndrome virus (PRRSV) vaccines induce slow development of effective immunity as measured by serum neutralizing (SN) antibody and cell-mediated immunity. The objective of the studies was to develop more effective vaccines or vaccination strategies based on commercial vaccines. The immune response and produced induced were characterized. Three separate studies were conducted to investigate the efficacy of vaccination protocols including vaccination with MLV and KV, either alone or in combination, KV with interleukin (IL)-12 added, and KV used as a booster vaccine in a PRRSV positive herd. The protection ...


Investigation Of Genetic Susceptibility To The Porcine Reproductive And Respiratory Syndrome Virus Between Commercial Breeding Stock Lines Of Pigs , Amy Louise Vincent Jan 2004

Investigation Of Genetic Susceptibility To The Porcine Reproductive And Respiratory Syndrome Virus Between Commercial Breeding Stock Lines Of Pigs , Amy Louise Vincent

Retrospective Theses and Dissertations

The objectives in the studies described herein were to evaluate the use of an antemortem in vitro test as an indicator in identifying commercial breeding stock lines with high and low susceptibility to PRRSV and to evaluate the pigs from lines selected from the first objective in an experimental infection model. Macrophages have been shown to be the cell type PRRSV preferentially infects and subsequently manipulates to achieve efficient replication and production of progeny virions. The in vitro assay was therefore designed to evaluate the percentage of macrophages derived from peripheral blood monocytes that are susceptible to infection in culture ...


The Identification And Characterization Of P5 And P2 Colonization Proteins In Haemophilus Parasuis And The Targeted Binding Of Carcinoembryonic Antigen (Cea) , Jerry Kim Mcvicker Jan 2004

The Identification And Characterization Of P5 And P2 Colonization Proteins In Haemophilus Parasuis And The Targeted Binding Of Carcinoembryonic Antigen (Cea) , Jerry Kim Mcvicker

Retrospective Theses and Dissertations

The outer membrane protein P5 from Haemophilus influenzae has been shown to aid colonization in the human nasopharynx by binding to CD66 (CEA) on the surface of mucosal epithelial cells. The intent here was to determine if Haemophilus parasuis contained homologs of the P5 and P2 proteins and to characterize these proteins by SDS-PAGE, IEF, and CEA binding. Using outer membrane protein P5 primers designed from the H. influenzae database, we were able to detect a single PCR product in a H. parasuis field isolate. The PCR product was transformed into a competent E. coli strain and sequenced. DNA sequence ...


Salmonella Enterica In Cull Sows And Meat From Cull Sows , Steven Thomas Larsen Jan 2004

Salmonella Enterica In Cull Sows And Meat From Cull Sows , Steven Thomas Larsen

Retrospective Theses and Dissertations

The overall objectives of this research were to first determine the overall prevalence of S. enterica in a given population of cull sows, secondly to apply control measures to lower the prevalence of S. enterica , thirdly was to use epidemiological tools to investigate the origin of S. enterica serovars isolated from the farm, live hog market, and abattoir, and lastly to determine invasiveness of S. enterica serovars isolated from cull sow feces, tissues, and environmental samples, as measured by an invasion assay.;The total average percent positive fecal samples at the farm, the live hog market, and abattoir were 3 ...


Quantification Of Salmonella From Porcine Samples To Identify Potential Critical Control Points In Pre-Harvest Pork Production , Jared Keith Gailey Jan 2004

Quantification Of Salmonella From Porcine Samples To Identify Potential Critical Control Points In Pre-Harvest Pork Production , Jared Keith Gailey

Retrospective Theses and Dissertations

This dissertation focuses on Salmonella in the pre-harvest portion of pork production. Nearly all of the current research in this area has focused on the prevalence of Salmonella in swine, with little attention being paid to quantifying positive sample. The purpose of this dissertation was to demonstrate the usefulness of quantitative data in identifying critical control points in pre-harvest pork production.;The current method for enumerating Salmonella, the most probable number method (MPN), is time consuming, labor intensive and cumbersome. In order to facilitate the acquisition of quantitative data, a new, less labor intensive, variation of the MPN method (the ...