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Full-Text Articles in Life Sciences

Analysis Of Papilloma Virus E1 Protein: Creation Of Stable Cell Lines To Generate Sufficient Amounts Of Viral E1, Peaches Marie Raval Orallo Jan 2004

Analysis Of Papilloma Virus E1 Protein: Creation Of Stable Cell Lines To Generate Sufficient Amounts Of Viral E1, Peaches Marie Raval Orallo

All Volumes (2001-2008)

The immediate objective of our research aims to create stable cell lines to generate sufficient amounts of viral E1. The pMSG vector was chosen for its ability to amplify gene expression by way of hormonal or chemical induction, and for its ability to be selected under ampicillin medium by way of the Amp gene. pMSG was ligated with E1 insert in two fashions, blunt or modified ends. Inserts were created by way of digesting them with BamH1. The inserts were blunted and ligated with a blunt ended pMSG, or blunted, modified, digested with Sal 1 and then ligated with Sal ...


Pathogenesis And Transmission Of Hepatitis E Virus (Hev) In Pigs, Chaiyan Kasorndorkbua Jan 2004

Pathogenesis And Transmission Of Hepatitis E Virus (Hev) In Pigs, Chaiyan Kasorndorkbua

Retrospective Theses and Dissertations

Human hepatitis E virus (HEV) was first reported in 1980 and is now considered a major cause of acute non-A, non-B hepatitis in humans. HEV infection is reported throughout the world and occurs in epidemic form in developing countries where the disease is endemic and is often associated with water contamination after heavy rains or flooding. Sporadic human HEV infections occur in industrialized countries where infected individuals contract the infection while traveling to endemic regions. The fecal-oral route is considered the primary mode of HEV transmission in humans. HEV infection of pigs was discovered in 1997. Since 1997, sporadic HEV ...


Investigation Of Immune Response And Protection Induced By A Combined Vaccination Of Modified Live And Killed Porcine Reproductive And Respiratory Syndrome Virus Vaccines And Its Potential Use In A Swine Herd , Dachrit Nilubol Jan 2004

Investigation Of Immune Response And Protection Induced By A Combined Vaccination Of Modified Live And Killed Porcine Reproductive And Respiratory Syndrome Virus Vaccines And Its Potential Use In A Swine Herd , Dachrit Nilubol

Retrospective Theses and Dissertations

Commercial modified live (MLV) and killed (KV) porcine reproductive and respiratory syndrome virus (PRRSV) vaccines induce slow development of effective immunity as measured by serum neutralizing (SN) antibody and cell-mediated immunity. The objective of the studies was to develop more effective vaccines or vaccination strategies based on commercial vaccines. The immune response and produced induced were characterized. Three separate studies were conducted to investigate the efficacy of vaccination protocols including vaccination with MLV and KV, either alone or in combination, KV with interleukin (IL)-12 added, and KV used as a booster vaccine in a PRRSV positive herd. The protection ...