Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Quantitative Signature For Architectural Organization Of Regulatory Factors Using Intranuclear Informatics, Daniel W. Young, Sayyed K. Zaidi, Paul S. Furcinitti, Amjad Javed, Andre J. Van Wijnen, Janet L. Stein, Jane B. Lian, Gary S. Stein Sep 2004

Quantitative Signature For Architectural Organization Of Regulatory Factors Using Intranuclear Informatics, Daniel W. Young, Sayyed K. Zaidi, Paul S. Furcinitti, Amjad Javed, Andre J. Van Wijnen, Janet L. Stein, Jane B. Lian, Gary S. Stein

GSBS Student Publications

Regulatory machinery for replication and gene expression is punctately organized in supramolecular complexes that are compartmentalized in nuclear microenvironments. Quantitative approaches are required to understand the assembly of regulatory machinery within the context of nuclear architecture and to provide a mechanistic link with biological control. We have developed 'intranuclear informatics' to quantify functionally relevant parameters of spatially organized nuclear domains. Using this informatics strategy we have characterized post-mitotic reestablishment of focal subnuclear organization of Runx (AML/Cbfa) transcription factors in progeny cells. By analyzing point mutations that abrogate fidelity of Runx intranuclear targeting, we establish molecular determinants for the spatial ...


Intranuclear Trafficking: Organization And Assembly Of Regulatory Machinery For Combinatorial Biological Control, Sayyed K. Zaidi, Daniel W. Young, Je-Yong Choi, Jitesh Pratap, Amjad Javed, Martin A. Montecino, Janet L. Stein, Jane B. Lian, Andre J. Van Wijnen, Gary S. Stein Jul 2004

Intranuclear Trafficking: Organization And Assembly Of Regulatory Machinery For Combinatorial Biological Control, Sayyed K. Zaidi, Daniel W. Young, Je-Yong Choi, Jitesh Pratap, Amjad Javed, Martin A. Montecino, Janet L. Stein, Jane B. Lian, Andre J. Van Wijnen, Gary S. Stein

GSBS Student Publications

The molecular logistics of nuclear regulatory processes necessitate temporal and spatial regulation of protein-protein and protein-DNA interactions in response to physiological cues. Biochemical, in situ, and in vivo genetic evidence demonstrates the requirement for intranuclear localization of regulatory complexes that functionally couple cellular responses to signals that mediate combinatorial control of gene expression. We have summarized evidence that subnuclear targeting of transcription factors mechanistically links gene expression with architectural organization and assembly of nuclear regulatory machinery for biological control. The compromised intranuclear targeting of regulatory proteins under pathological conditions provides options for the diagnosis and treatment of disease.


Nuclear Microenvironments: An Architectural Platform For The Convergence And Integration Of Transcriptional Regulatory Signals, Gary S. Stein, Janet L. Stein, Jane B. Lian, Andre J. Van Wijnen, Martin A. Montecino, Amjad Javed, Sayyed K. Zaidi, Daniel W. Young, Je-Yong Choi, Shirwin M. Pockwinse May 2004

Nuclear Microenvironments: An Architectural Platform For The Convergence And Integration Of Transcriptional Regulatory Signals, Gary S. Stein, Janet L. Stein, Jane B. Lian, Andre J. Van Wijnen, Martin A. Montecino, Amjad Javed, Sayyed K. Zaidi, Daniel W. Young, Je-Yong Choi, Shirwin M. Pockwinse

GSBS Student Publications

Functional interrelationships between the intranuclear organization of nucleic acids and regulatory proteins are obligatory for fidelity of transcriptional activation and repression. In this article, using the Runx/AML/Cbfa transcription factors as a paradigm for linkage between nuclear structure and gene expression we present an overview of growing insight into the dynamic organization and assembly of regulatory machinery for gene expression at microenvironments within the nucleus. We address contributions of nuclear microenvironments to the convergence and integration of regulatory signals that mediate transcription by supporting the combinatorial assembly of regulatory complexes.