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Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

2004

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Biochemistry, Biophysics, and Structural Biology

Development and cell biology

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

The Central Role Of Acetyl-Coa In Plant Metabolism, As Examined Through Studies Of Atp Citrate Lyase And The Bio1 Mutant Of Arabidopsis , Elizabeth K. Winters Jan 2004

The Central Role Of Acetyl-Coa In Plant Metabolism, As Examined Through Studies Of Atp Citrate Lyase And The Bio1 Mutant Of Arabidopsis , Elizabeth K. Winters

Retrospective Theses and Dissertations

The acetyl-CoA/biotin network encompasses acetyl-CoA generation and utilization, biotin synthesis, recovery, and transport, and myriad metabolic processes requiring biotin-containing enzymes. To expand understanding of this network and its control, plants with altered levels of ACL (ATP citrate lyase) and BIO1 (adenosylmethionine-8-amino-7-oxononanoate synthase) were examined.;An inducible promoter provides a powerful method to test the effects of altered expression of specific genes. The synthetic GVG promoter, reported to be sterol-inducible in Arabidopsis thaliana, was inducible in Glycine max (soybean) when used to control the GUS reporter gene, or the ACLA or ACLB genes. It showed the same unique pattern in ...


Computational And Molecular Analysis Of Myb Gene Family, Cizhong Jiang Jan 2004

Computational And Molecular Analysis Of Myb Gene Family, Cizhong Jiang

Retrospective Theses and Dissertations

Myb proteins are defined by a highly conserved DNA-specific binding domain termed Myb, which is composed of approximately 50 amino acids with constantly spaced tryptophan residues. Multiple copies of Myb domains often exist as tandem repeats within a single protein. There are up to four tandem Myb repeats present in Myb proteins identified to date (termed R0R1R2R3 hereafter).;In our study, we collected additional Myb genes, and performed a series of phylogenetic analyses to explore the evolutionary origin of Myb genes. The results suggest that the Myb gene family originated from an ancient one Myb-box gene. One and two intragenic ...


Investigation Of The Molecular Mechanism Of Fertility Restoration In Texas Cytoplasm Maize , David Stewart Skibbe Jan 2004

Investigation Of The Molecular Mechanism Of Fertility Restoration In Texas Cytoplasm Maize , David Stewart Skibbe

Retrospective Theses and Dissertations

Cytoplasmic male sterility (cms) is a maternally inherited inability to produce viable pollen. In Texas (T)-cytoplasm maize, the combined action of two restorer of fertility genes, rfl and rfla , can overcome the male sterile phenotype. Previous research established rf2a encodes a mitochondrial aldehyde dehydrogenase (ALDH) with broad substrate specificity. This broad substrate specificity complicates determining the molecular mechanism of rf2a-mediated fertility restoration. In response, a combination of transgenic, biochemical, reverse genetic, and genomic approaches were utilized. Three additional functional maize ALDH genes (rf2b, rf2c, and rf2d) with differing substrate specificities were identified. Each ALDH gene was characterized via in ...