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2009

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Full-Text Articles in Life Sciences

Joint Fire Science Program – Lake Mead National Recreation Area Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis: Quarterly Progress Report, Time Period: October 1 — December 31, 2009, Margaret N. Rees Dec 2009

Joint Fire Science Program – Lake Mead National Recreation Area Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis: Quarterly Progress Report, Time Period: October 1 — December 31, 2009, Margaret N. Rees

Fire Science

• Article accepted for publication in Journal of Arid Environments.

• Recharged irrigation gel packs for outplanting and watered seed plots at Goodsprings site.

• Completed full year of seed granivory trials at Goodsprings site.

• Presented information on this Task Agreement to 4th International Fire Ecology and Management Congress.

• Maintained nursery plots and added installment of nitrogen treatment.


Lake Mead National Recreation Area Sensitive Wildlife Species Monitoring And Analysis: Quarterly Progress Report, Period Ending December 31, 2009, Margaret N. Rees Dec 2009

Lake Mead National Recreation Area Sensitive Wildlife Species Monitoring And Analysis: Quarterly Progress Report, Period Ending December 31, 2009, Margaret N. Rees

Wildlife Monitoring

Project 1. Relict Leopard Frog Monitoring, Management, and Research

  • All milestones and deliverables are on schedule
  • Fall surveys at all sites have been completed
  • Mark-recapture surveys scheduled for fall were completed
  • Short-term habitat improvements at two sites were conducted
  • RLFCT meeting was hosted
  • Draft annual report was written and presented at the RLFCT meeting

Project 2. Bald Eagle Winter Monitoring and Evaluation

  • All milestones and deliverables are on schedule
  • An annual project review presentation was given to Clark County
  • A draft final report was written and submitted to Clark County
  • All data for this project was transferred to the County ...


Investing In Maine Research Infrastructure: Sustainable Forest Bioproducts, Michael Eckardt, Stephen Shaler, Hemant P. Pendse, Adriaan R. P. Van Heiningen, Robert G. Wagner Dec 2009

Investing In Maine Research Infrastructure: Sustainable Forest Bioproducts, Michael Eckardt, Stephen Shaler, Hemant P. Pendse, Adriaan R. P. Van Heiningen, Robert G. Wagner

University of Maine Office of Research Administration: Grant Reports

The University of Maine, the University of Southern Maine, several baccalaureate institutions in the state, along with other federal, state and local public, private, and non-profit institutions will collaborate to create the Forest Bioproducts Research Institute (FBRI) at the University of Maine. The vision of the FBRI is to advance understanding about the scientific underpinnings, system behavior, and policy implications for the production of forest-based bioproducts that meet societal needs for materials, chemicals, and fuels in an economically and ecologically sustainable manner.

The research plans Integrate three themes. They are (1) forest sustainability modeling of life cycle assessment, (2) integrated ...


Anomalous Constitutive Src Kinase Activity Promotes B Lymphoma Survival And Growth, Jiyuan Ke, R. Lakshman Chelvarajan, Vishal Sindhava, Darrell A. Robertson, Lazaros Lekakis, C. Darrell Jennings, Subbarao Bondada Dec 2009

Anomalous Constitutive Src Kinase Activity Promotes B Lymphoma Survival And Growth, Jiyuan Ke, R. Lakshman Chelvarajan, Vishal Sindhava, Darrell A. Robertson, Lazaros Lekakis, C. Darrell Jennings, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Background: Previously we have shown that B cell receptor (BCR) expression and B cell receptor signaling pathways are important for the basal growth of B lymphoma cells. In particular we have shown that the activation of Syk, a non-src family protein tyrosine kinase and the mitogen activated protein kinases (MAPK), ERK and JNK that mediate BCR signals are required for the constitutive growth of B lymphoma cells. Since src family protein tyrosine kinases (SFKs) like Lyn are known to be needed for the phosphorylation of BCR co-receptors, Ig-alpha and Ig-beta, we hypothesized that one or more SFKs will be constitutively ...


A Constant Light-Genetic Screen Identifies Kismet As A Regulator Of Circadian Photoresponses, Raphaëlle Dubruille, Alejandro D. Murad, Michael Rosbash, Patrick Emery Dec 2009

A Constant Light-Genetic Screen Identifies Kismet As A Regulator Of Circadian Photoresponses, Raphaëlle Dubruille, Alejandro D. Murad, Michael Rosbash, Patrick Emery

Open Access Articles

Circadian pacemakers are essential to synchronize animal physiology and behavior with the dayrationight cycle. They are self-sustained, but the phase of their oscillations is determined by environmental cues, particularly light intensity and temperature cycles. In Drosophila, light is primarily detected by a dedicated blue-light photoreceptor: CRYPTOCHROME (CRY). Upon light activation, CRY binds to the pacemaker protein TIMELESS (TIM) and triggers its proteasomal degradation, thus resetting the circadian pacemaker. To understand further the CRY input pathway, we conducted a misexpression screen under constant light based on the observation that flies with a disruption in the CRY input pathway remain robustly rhythmic ...


The Acidic Domains Of The Toc159 Chloroplast Preprotein Receptor Family Are Instrinsically Disordered Protein Domains, Lynn G.L. Richardson, Masoud Jelokhani-Niaraki, Matthew D. Smith Dec 2009

The Acidic Domains Of The Toc159 Chloroplast Preprotein Receptor Family Are Instrinsically Disordered Protein Domains, Lynn G.L. Richardson, Masoud Jelokhani-Niaraki, Matthew D. Smith

Biology Faculty Publications

Background: The Toc159 family of proteins serve as receptors for chloroplast-destined preproteins. They directly bind to transit peptides, and exhibit preprotein substrate selectivity conferred by an unknown mechanism. The Toc159 receptors each include three domains: C-terminal membrane, central GTPase, and N-terminal acidic (A-) domains. Although the function(s) of the A-domain remains largely unknown, the amino acid sequences are most variable within these domains, suggesting they may contribute to the functional specificity of the receptors.

Results: The physicochemical properties of the A-domains are characteristic of intrinsically disordered proteins (IDPs). Using CD spectroscopy we show that the A-domains of two Arabidopsis ...


The Chlamydomonas Reinhardtii Bbsome Is An Ift Cargo Required For Export Of Specific Signaling Proteins From Flagella, Karl-Ferdinand Lechtreck, Eric C. Johnson, Tsuyoshi Sakai, Deborah A. Cochran, Bryan A. Ballif, John Rush, Gregory J. Pazour, Mitsuo Ikebe, George B. Witman Dec 2009

The Chlamydomonas Reinhardtii Bbsome Is An Ift Cargo Required For Export Of Specific Signaling Proteins From Flagella, Karl-Ferdinand Lechtreck, Eric C. Johnson, Tsuyoshi Sakai, Deborah A. Cochran, Bryan A. Ballif, John Rush, Gregory J. Pazour, Mitsuo Ikebe, George B. Witman

Witman Lab Publications

In humans, seven evolutionarily conserved genes that cause the cilia-related disorder Bardet-Biedl syndrome (BBS) encode proteins that form a complex termed the BBSome. The function of the BBSome in the cilium is not well understood. We purified a BBSome-like complex from Chlamydomonas reinhardtii flagella and found that it contains at least BBS1, -4, -5, -7, and -8 and undergoes intraflagellar transport (IFT) in association with a subset of IFT particles. C. reinhardtii insertional mutants defective in BBS1, -4, and -7 assemble motile, full-length flagella but lack the ability to phototax. In the bbs4 mutant, the assembly and transport of IFT ...


Recommendations For Managing Soybean White Mold In 2010, Xiao-Bing Yang Dec 2009

Recommendations For Managing Soybean White Mold In 2010, Xiao-Bing Yang

Integrated Crop Management News

The 2009 growing season had record cool weather in July that was ideal for soybean white mold occurrence. The disease was widespread in the north central region of the U.S. and agronomists even in southern Iowa observed this disease in many soybean fields. In northern Iowa, patches of soybean killed by this disease were obvious in many soybean fields along the highways. Some farmers reported losses totaling more than $10,000 from this disease.


The Principled Control Of False Positives In Neuroimaging, Craig M. Bennett, George L. Wolford, Michael B. Miller Dec 2009

The Principled Control Of False Positives In Neuroimaging, Craig M. Bennett, George L. Wolford, Michael B. Miller

Open Dartmouth: Faculty Open Access Scholarship

An incredible amount of data is generated in the course of a functional neuroimaging experiment. The quantity of data gives us improved temporal and spatial resolution with which to evaluate our results. It also creates a staggering multiple testing problem. A number of methods have been created that address the multiple testing problem in neuroimaging in a principled fashion. These methods place limits on either the familywise error rate (FWER) or the false discovery rate (FDR) of the results. These principled approaches are well established in the literature and are known to properly limit the amount of false positives across ...


Mir319a Targeting Of Tcp4 Is Critical For Petal Growth And Development In Arabidopsis, Anwesha Nag, Stacey King, Thomas Jack Dec 2009

Mir319a Targeting Of Tcp4 Is Critical For Petal Growth And Development In Arabidopsis, Anwesha Nag, Stacey King, Thomas Jack

Open Dartmouth: Faculty Open Access Scholarship

In a genetic screen in a drnl-2 background, we isolated a loss-of-function allele in miR319a (miR319a129). Previously, miR319a has been postulated to play a role in leaf development based on the dramatic curled-leaf phenotype of plants that ectopically express miR319a (jaw-D). miR319a129 mutants exhibit defects in petal and stamen development; petals are narrow and short, and stamens exhibit defects in anther development. The miR319a129 loss-of-function allele contains a single-base change in the middle of the encoded miRNA, which reduces the ability of miR319a to recognize targets. Analysis of the expression patterns of the three members of the ...


Rearrangement Of The Rna Polymerase Subunit H And The Lower Jaw In Archaeal Elongation Complexes, Sebastian Grünberg, Christoph Reich, Mirijam E. Zeller, Michael S. Bartlett, Michael Thomm Dec 2009

Rearrangement Of The Rna Polymerase Subunit H And The Lower Jaw In Archaeal Elongation Complexes, Sebastian Grünberg, Christoph Reich, Mirijam E. Zeller, Michael S. Bartlett, Michael Thomm

Biology Faculty Publications and Presentations

The lower jaws of archaeal RNA polymerase and eukaryotic RNA polymerase II include orthologous subunits H and Rpb5, respectively. The tertiary structure of H is very similar to the structure of the C-terminal domain of Rpb5, and both subunits are proximal to downstream DNA in pre-initiation complexes. Analyses of reconstituted euryarchaeal polymerase lacking subunit H revealed that H is important for open complex formation and initial transcription. Eukaryotic Rpb5 rescues activity of the ΔH enzyme indicating a strong conservation of function for this subunit from archaea to eukaryotes. Photochemical cross-linking in elongation complexes revealed a striking structural rearrangement of RNA ...


Proteolytic Regulation Of Toxin-Antitoxin Systems By Clppc In Staphylococcus Aureus, Niles P. Donegan, Earl T. Thompson, Zhibiao Fu, Ambrose L. Cheung Dec 2009

Proteolytic Regulation Of Toxin-Antitoxin Systems By Clppc In Staphylococcus Aureus, Niles P. Donegan, Earl T. Thompson, Zhibiao Fu, Ambrose L. Cheung

Open Dartmouth: Faculty Open Access Scholarship

Bacterial toxin-antitoxin (TA) systems typically consist of a small, labile antitoxin that inactivates a specific longer-lived toxin. In Escherichia coli, such antitoxins are proteolytically regulated by the ATP-dependent proteases Lon and ClpP. Under normal conditions, antitoxin synthesis is sufficient to replace this loss from proteolysis, and the bacterium remains protected from the toxin. However, if TA production is interrupted, antitoxin levels decrease, and the cognate toxin is free to inhibit the specific cellular component, such as mRNA, DnaB, or gyrase. To date, antitoxin degradation has been studied only in E. coli, so it remains unclear whether similar mechanisms of regulation ...


Sympatric Ecological Speciation Meets Pyrosequencing: Sampling The Transcriptome Of The Apple Maggot Rhagoletis Pomonella, Dietmar Schwarz, Hugh M. Robertson, Jeffrey L. Feder, Kranthi Varala, Matthew E. Hudson, Gregory J. Ragland, Daniel A. Hahn, Stewart H. Berlocher Dec 2009

Sympatric Ecological Speciation Meets Pyrosequencing: Sampling The Transcriptome Of The Apple Maggot Rhagoletis Pomonella, Dietmar Schwarz, Hugh M. Robertson, Jeffrey L. Feder, Kranthi Varala, Matthew E. Hudson, Gregory J. Ragland, Daniel A. Hahn, Stewart H. Berlocher

Biology Faculty and Staff Publications

Background

The full power of modern genetics has been applied to the study of speciation in only a small handful of genetic model species - all of which speciated allopatrically. Here we report the first large expressed sequence tag (EST) study of a candidate for ecological sympatric speciation, the apple maggot Rhagoletis pomonella, using massively parallel pyrosequencing on the Roche 454-FLX platform. To maximize transcript diversity we created and sequenced separate libraries from larvae, pupae, adult heads, and headless adult bodies.

Results

We obtained 239,531 sequences which assembled into 24,373 contigs. A total of 6810 unique protein coding genes ...


2008-2009 Annual Report Dec 2009

2008-2009 Annual Report

Annual Report Archive from UNL Extension in Lancaster County

Know how. Know now.
15-to-1 Return on Investment
2009 Program Highlights
Research Confirms 4-H Youth Are Leaders and Contribute to Our Community
From NU to You
http://lancaster.unl.edu is Your 24/7 Local Extension Connection
4-H Youth Learn Life Skills Through Learning-by-Doing
AmericCorps Member is part of Go-Green Initiative
Honors, Awards and Accomplishments
Lancaster Extension Education Center Conference Facilities
Yard & Garden
Plant and Pest Diagnostic Services
Pests & Wildlife
Home & Family Living
Farms & Acreages
Nutrition & Food Safety
Family & Community Education (FCE) Clubs


A Unique Role For The Host Escrt Proteins In Replication Of Tomato Bushy Stunt Virus, Daniel Barajas, Yi Jiang, Peter D. Nagy Dec 2009

A Unique Role For The Host Escrt Proteins In Replication Of Tomato Bushy Stunt Virus, Daniel Barajas, Yi Jiang, Peter D. Nagy

Plant Pathology Faculty Publications

Plus-stranded RNA viruses replicate in infected cells by assembling viral replicase complexes consisting of viral- and host-coded proteins. Previous genome-wide screens with Tomato bushy stunt tombusvirus (TBSV) in a yeast model host revealed the involvement of seven ESCRT (endosomal sorting complexes required for transport) proteins in viral replication. In this paper, we show that the expression of dominant negative Vps23p, Vps24p, Snf7p, and Vps4p ESCRT factors inhibited virus replication in the plant host, suggesting that tombusviruses co-opt selected ESCRT proteins for the assembly of the viral replicase complex. We also show that TBSV p33 replication protein interacts with Vps23p ESCRT-I ...


Northeast Research Station Watertown, South Dakota Annual Progress Report, 2009, Agricultural Experiment Station, Plant Science Department Dec 2009

Northeast Research Station Watertown, South Dakota Annual Progress Report, 2009, Agricultural Experiment Station, Plant Science Department

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2009 annual progress report for the Northeast Research Station in Watertown, South Dakota. This report is issued by the South Dakota Agricultural Experiment Station and the South Dakota State University Plant Science Department. This report includes weather data, yield comparisons, crop performance trials, corn trials, soybean trials, winter wheat performance testing, Barley Foliar information, weed and pest control, herbicide demonstrations, canola and flax variety trials and more.


Economic And Other Barriers To Adopting Recommendations To Prevent Childhood Obesity: Results Of A Focus Group Study With Parents, Kendrin R. Sonneville, Nancy R. Lapelle, Elsie M. Taveras, Matthew W. Gillman, Lisa A. Prosser Dec 2009

Economic And Other Barriers To Adopting Recommendations To Prevent Childhood Obesity: Results Of A Focus Group Study With Parents, Kendrin R. Sonneville, Nancy R. Lapelle, Elsie M. Taveras, Matthew W. Gillman, Lisa A. Prosser

Open Access Articles

BACKGROUND: Parents are integral to the implementation of obesity prevention and management recommendations for children. Exploration of barriers to and facilitators of parental decisions to adopt obesity prevention recommendations will inform future efforts to reduce childhood obesity.

METHODS: We conducted 4 focus groups (2 English, 2 Spanish) among a total of 19 parents of overweight (BMI >or= 85th percentile) children aged 5-17 years. The main discussion focused on 7 common obesity prevention recommendations: reducing television (TV) watching, removing TV from child's bedroom, increasing physically active games, participating in community or school-based athletics, walking to school, walking more in general ...


Crop Advantage Series Provides Latest Crop Production Information, Brent A. Pringnitz Dec 2009

Crop Advantage Series Provides Latest Crop Production Information, Brent A. Pringnitz

Integrated Crop Management News

If you’re involved in crop production in Iowa, plan to attend one of the 2010 Crop Advantage Series meetings near you. Sponsored by Iowa State University Extension, the meetings will be held at 13 locations across Iowa in January.


Chromatin 'Programming' By Sequence - Is There More To The Nucleosome Code Than %Gc, Amanda Hughes, Oliver J. Rando Dec 2009

Chromatin 'Programming' By Sequence - Is There More To The Nucleosome Code Than %Gc, Amanda Hughes, Oliver J. Rando

Open Access Articles

The role of genomic sequence in directing the packaging of eukaryotic genomes into chromatin has been the subject of considerable recent debate. A new paper from Tillo and Hughes shows that the intrinsic thermodynamic preference of a given sequence in the yeast genome for the histone octamer can largely be captured with a simple model, and in fact is mostly explained by %GC. Thus, the rules for predicting nucleosome occupancy from genomic sequence are much less complicated than has been claimed.


Nuclear Receptor Unfulfilled Regulates Axonal Guidance And Cell Identity Of Drosophila Mushroom Body Neurons, Suewei Lin, Yaling Huang, Tzumin Lee Dec 2009

Nuclear Receptor Unfulfilled Regulates Axonal Guidance And Cell Identity Of Drosophila Mushroom Body Neurons, Suewei Lin, Yaling Huang, Tzumin Lee

Open Access Articles

Nuclear receptors (NRs) comprise a family of ligand-regulated transcription factors that control diverse critical biological processes including various aspects of brain development. Eighteen NR genes exist in the Drosophila genome. To explore their roles in brain development, we knocked down individual NRs through the development of the mushroom bodies (MBs) by targeted RNAi. Besides recapitulating the known MB phenotypes for three NRs, we found that unfulfilled (unf), an ortholog of human photoreceptor specific nuclear receptor (PNR), regulates axonal morphogenesis and neuronal subtype identity. The adult MBs develop through remodeling of gamma neurons plus de-novo elaboration of both alpha'/beta' and ...


Crop And Weather Report Dec. 21, Integrated Crop Management News Dec 2009

Crop And Weather Report Dec. 21, Integrated Crop Management News

Integrated Crop Management News

Crop and weather report guests include Iowa State University Extension climatologist Elwynn Taylor, integrated pest management specialist Rich Pope, and corn agronomist Roger Elmore. On the first day of winter with snow covering most of the state and halting any remaining corn harvests, guests comment on the just released corn yield report - 2009 Iowa Crop Performance Test --- Corn.


Vitisnet: “Omics” Integration Through Grapevine Molecular Networks, Jérôme Grimplet, Grant R. Cramer, Julie Dickerson, Kathy Mathiason, John L. Van Hemert, Anne Y. Fennell Dec 2009

Vitisnet: “Omics” Integration Through Grapevine Molecular Networks, Jérôme Grimplet, Grant R. Cramer, Julie Dickerson, Kathy Mathiason, John L. Van Hemert, Anne Y. Fennell

Electrical and Computer Engineering Publications

Background

Genomic data release for the grapevine has increased exponentially in the last five years. The Vitis vinifera genome has been sequenced and Vitis EST, transcriptomic, proteomic, and metabolomic tools and data sets continue to be developed. The next critical challenge is to provide biological meaning to this tremendous amount of data by annotating genes and integrating them within their biological context. We have developed and validated a system of Grapevine Molecular Networks (VitisNet).

Methodology/Principal Findings

The sequences from the Vitis vinifera (cv. Pinot Noir PN40024) genome sequencing project and ESTs from the Vitis genus have been paired and ...


Fate And Bioavailability Of Sulfamethazine In Freshwater Ecosystems, Keri L. D. Henderson, Thomas Moorman, Joel R. Coats Dec 2009

Fate And Bioavailability Of Sulfamethazine In Freshwater Ecosystems, Keri L. D. Henderson, Thomas Moorman, Joel R. Coats

Entomology Publications

The antibiotic sulfamethazine can be transported from manured fields to surface water bodies. We investigated the degradation, fate, and bioavailability of sulfamethazine in surface water using 14C-U-phenyl-sulfamethazine in small pond water microcosms. Sulfamethazine dissipated exponentially from the water column, with the majority of loss occurring via movement into the sediment phase. Manure input significantly increased sorption and binding of sulfamethazine residues to the sediment. These results indicate sediment is a potential sink for sulfamethazine and sulfamethazine-related residues, which could have important implications for benthic organisms. Understanding the bioavailability of pharmaceuticals in environmental matrices is particularly important considering they are often ...


Veterinary Pharmaceuticals In The Environment: An Introduction, Keri L. D. Hendersn, Joel R. Coats Dec 2009

Veterinary Pharmaceuticals In The Environment: An Introduction, Keri L. D. Hendersn, Joel R. Coats

Entomology Publications

The production of swine, cattle, and poultry raised for human consumption represents a significant portion of the U.S. agricultural economy. To maximize production, livestock producers regularly use pharmaceuticals and hormones as supplements in animal feed and water to increase rates of weight gain, and prevent or treat diseases among their livestock. For example, in swine it is estimated that antibiotics are used for disease prevention and growth promotion in more than 90% of starter feeds, 75% of grower feeds, 50% of finishing feeds, and 20% of sow feeds. Equally relevant numbers are seen in beef cattle production (1-4). It ...


Environmental Fate And Chemistry Of A Veterinary Antibiotic—Tylosin, Dingfei Hu, Keri L. D. Henderson, Joel R. Coats Dec 2009

Environmental Fate And Chemistry Of A Veterinary Antibiotic—Tylosin, Dingfei Hu, Keri L. D. Henderson, Joel R. Coats

Entomology Publications

Aerobic degradation, photolysis, and mobility of tylosin were investigated in the laboratory. Tylosin A is degraded with a half-life of 200 d in water, while it is stable in the dark. Tylosin C and D are relatively stable except in ultrapure water in the light. Slight increases of tylosin B and formation of two photoreaction products, isotylosin A alcohol (E,Z) and isotylosin A aldol (E,Z), were observed under exposure to light. In soil tylosin A and D has a dissipation half-life of about 1 wk. Sorption and abiotic degradation are the major factors influencing the loss of tylosin ...


Preface Of Veterinary Pharmaceuticals In The Environment, Keri Henderson, Joel R. Coats Dec 2009

Preface Of Veterinary Pharmaceuticals In The Environment, Keri Henderson, Joel R. Coats

Entomology Publications

An important symposium was presented on "Veterinary Pharmaceuticals in the Environment" at the 233rd National Meeting of the American Chemical Society on March 25-26, 2007, in Chicago, IL. The excellence and timeliness of the presentations indicated the need for an ACS Symposium Series book addressing the environmental chemistry and toxicology of this group of emerging contaminants.

The purpose of this symposium was to bring together scientists from academia, government, and industry to discuss and present data relevant to the significance of veterinary pharmaceuticals in the environment. A broad range of topics was covered, including environmental chemistry studies focusing on transport ...


Amyris And Siam-Wood Essential Oils: Insect Activity Of Sesquiterpenes, Gretchen E. Paluch, Junwei Zhu, Lyric Bartholomay, Joel R. Coats Dec 2009

Amyris And Siam-Wood Essential Oils: Insect Activity Of Sesquiterpenes, Gretchen E. Paluch, Junwei Zhu, Lyric Bartholomay, Joel R. Coats

Entomology Publications

Recent investigations on the sesquiterpene-rich Amyris (Amyris balsamifera L.) and Siam-wood (Fokienia hodginsii L.) essential oils revealed significant arthropod repellency and toxicity responses. Amyris essential oil and one of its major components, elemol, were evaluated in laboratory bioassays and identified as effective mosquito repellents, specifically characterized by high levels of contact and minimal spatial repellency. Mosquito responses to catnip (Nepeta cataria L.) essential oil are characterized with high spatial activity, but lack significant contact repellency. Sampling within the staticair bioassay chamber with solid-phase microextraction provided measurements of the relative concentration and distribution of volatiles. These results supported the differences observed ...


Maternal And Zygotic Aldh1a2 Activity Is Required For Pancreas Development In Zebrafish, Kristen Alexa, Seong-Kyu Choe, Nicolas Hirsch, Letitiah Etheridge, Elizabeth Laver, Charles G. Sagerstrom Dec 2009

Maternal And Zygotic Aldh1a2 Activity Is Required For Pancreas Development In Zebrafish, Kristen Alexa, Seong-Kyu Choe, Nicolas Hirsch, Letitiah Etheridge, Elizabeth Laver, Charles G. Sagerstrom

Open Access Articles

We have isolated and characterized a novel zebrafish pancreas mutant. Mutant embryos lack expression of isl1 and sst in the endocrine pancreas, but retain isl1 expression in the CNS. Non-endocrine endodermal gene expression is less affected in the mutant, with varying degrees of residual expression observed for pdx1, carbA, hhex, prox1, sid4, transferrin and ifabp. In addition, mutant embryos display a swollen pericardium and lack fin buds. Genetic mapping revealed a mutation resulting in a glycine to arginine change in the catalytic domain of the aldh1a2 gene, which is required for the production of retinoic acid from vitamin A. Comparison ...


Graduate Education In Research Ethics For Scientists And Engineers: Final Report, Jorge Ferrer-Negron, William Frey, Efrain O'Neill-Carrillo, Didier Valdes, Carlos Rios-Valazquez Dec 2009

Graduate Education In Research Ethics For Scientists And Engineers: Final Report, Jorge Ferrer-Negron, William Frey, Efrain O'Neill-Carrillo, Didier Valdes, Carlos Rios-Valazquez

Ethics in Science and Engineering National Clearinghouse

No abstract provided.


Tree Mortality Following Prescribed Fire And A Storm Surge Event In Slash Pine (Pinus Elliottii Var. Densa) Forests In The Florida Keys, Usa, Jay P. Sah, Michael S. Ross, James R. Snyder, Danielle E. Ogurcak Dec 2009

Tree Mortality Following Prescribed Fire And A Storm Surge Event In Slash Pine (Pinus Elliottii Var. Densa) Forests In The Florida Keys, Usa, Jay P. Sah, Michael S. Ross, James R. Snyder, Danielle E. Ogurcak

Department of Earth and Environment

In fire-dependent forests, managers are interested in predicting the consequences of prescribed burning on postfire tree mortality. We examined the effects of prescribed fire on tree mortality in Florida Keys pine forests, using a factorial design with understory type, season, and year of burn as factors. We also used logistic regression to model the effects of burn season, fire severity, and tree dimensions on individual tree mortality. Despite limited statistical power due to problems in carrying out the full suite of planned experimental burns, associations with tree and fire variables were observed. Post-fire pine tree mortality was negatively correlated with ...