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Developing A New Sensing Technology For Double-Stranded Dna Detection Utilizing Engineered Zinc Finger Proteins And Nanomaterials, Dat Thinh Ha Oct 2018

Developing A New Sensing Technology For Double-Stranded Dna Detection Utilizing Engineered Zinc Finger Proteins And Nanomaterials, Dat Thinh Ha

Masters Theses & Specialist Projects

A specific double-stranded DNA sensing system is of great interest for diagnostic and other biomedical applications. Zinc finger domains, which recognize double-stranded DNA, can be engineered to form custom DNA-binding proteins for recognition of specific DNA sequences. As a proof of concept, a sequence-enabled reassembly of TEM-1 β- lactamase system (SEER-LAC) was previously demonstrated to develop zinc finger protein (ZFP) arrays for the detection of a double-stranded bacterial DNA sequence. Here, we implemented the SEER-LAC system to demonstrate the direct detection of pathogenspecific DNA sequences present in E. coli O157:H7 on the lab-on-a chip. ZFPs customdesigned to detect shiga ...


Investigations Of Biotremors In The Veiled Chameleon (Chamaeleo Calyptratus), Kathryn C. Laslie Jul 2018

Investigations Of Biotremors In The Veiled Chameleon (Chamaeleo Calyptratus), Kathryn C. Laslie

Masters Theses & Specialist Projects

While substrate-borne vibrations are utilized by different reptile species, true conspecific communication via biotremors has not yet been demonstrated in reptiles. This study follows a preliminary report that the veiled chameleon (Chamaeleo calyptratus) could produce biotremors in communicative contexts. I tested chameleon behavioral sensitivity to vibrations by placing them on a dowel attached to a shaker emitting vibrations of 25, 50, 150, 300, and 600 Hz and then measured their changes in velocity before and after the stimulus. I then paired chameleons in various social contexts [anthropogenic disturbance (human disruption of animal); dominance (malemale; female-female C. calyptratus); courtship (male-female C ...


An Evaluation Of Deterrent Methods Utilized To Prevent Crop Raiding By African Elephants (Loxodonta Africana) In The Kasigau Wildlife Corridor, Kenya, Rebecca Lynn Von Hagen Jul 2018

An Evaluation Of Deterrent Methods Utilized To Prevent Crop Raiding By African Elephants (Loxodonta Africana) In The Kasigau Wildlife Corridor, Kenya, Rebecca Lynn Von Hagen

Masters Theses & Specialist Projects

Escalating human elephant conflict (HEC) continues to be a contributing factor

towards elephant decline, and crop raiding is the most common form of negative

human-elephant interactions. For communities that cannot reverse or prevent crop

raiding, it is necessary to contain HEC events through deterrent measures. Few

deterrent measures exist that combine practicality and affordability while also

preventing habituation by elephants. This project focused on comparing the efficacy of

deterrent methods to assess which was the most successful at preventing elephants

from entering crops in the farming community of Sasenyi, Kenya. In this paired-control

study, four deterrent methods were evaluated: acacia ...


Biomimetic Studies Of Oxidation Reactions By Metalloporphyrins Through Ligand Effect And Kinetic Studies Of Photo-Generated Porphyrin-Iron(Iv)- Oxocompound Ii Models, Dharmesh J. Patel Apr 2018

Biomimetic Studies Of Oxidation Reactions By Metalloporphyrins Through Ligand Effect And Kinetic Studies Of Photo-Generated Porphyrin-Iron(Iv)- Oxocompound Ii Models, Dharmesh J. Patel

Masters Theses & Specialist Projects

High-valent iron(IV)-oxo porphyrins are the central oxidizing species in hemecontaining enzymes and synthetic oxidation catalysts. Many transition metal complexes have been extensively studied as models of the ubiquitous cytochrome P450 enzymes to probe the sophisticated oxygen atom transfer (OAT) mechanism as well as to invent enzyme-like oxidation catalysts. In this work, two metalloporphyrin complexes have been successfully synthesized, and spectroscopically characterized. A new photochemical entry to porphyrin-iron(IV)-oxo derivatives, commonly referred to as compound II models, was also investigated in two porphyrin ligands that differ in electronic and steric environments. As determined by their distinct UV-vis spectra ...


Landscape Genetics Of The California Tiger Salamander: Inferences From Multiple Methods, Samantha Gabrielle Thomas Oct 2017

Landscape Genetics Of The California Tiger Salamander: Inferences From Multiple Methods, Samantha Gabrielle Thomas

Masters Theses & Specialist Projects

Landscape genetics is a rapidly growing field of study that compares patterns of gene flow among populations with habitat heterogeneity across a landscape to infer the interaction between dispersal of individuals and their physical environment. Empirical data generated from a landscape genetics study can inform conservation and management strategies, making the field increasing popular. However, concerns have arisen in the literature that the field is expanding faster than the analytic framework that supports it. Multiple methods for generating estimates of the association among habitat types and dispersal (i.e., least-cost paths and resistance surfaces) have been proposed, and there is ...


Effects Of Binding Affinity Between Bovine Serum Albumin And Platinum Drugs, Nathan Puckett Apr 2017

Effects Of Binding Affinity Between Bovine Serum Albumin And Platinum Drugs, Nathan Puckett

Masters Theses & Specialist Projects

Platinum complex drugs such as cisplatin have been used as highly successful chemotherapy drugs since the 1970s. We are interested in how the ligands attached to cisplatin analogs influences their reactivity with biologically relevant targets along with time and amount. For this study, reactions were conducted to determine the reactivity between different platinum compounds and the protein bovine serum albumin. Various platinum compounds with different ligands were reacted in varying amounts with albumin in ammonium acetate buffer for either 1 hour, 4 hours, or 24 hours. Each reaction was quenched after the designated reaction time by dialysis and the platinum ...


A Proposed Frequency-Based Feature Selection Method For Cancer Classification, Yi Pan Apr 2017

A Proposed Frequency-Based Feature Selection Method For Cancer Classification, Yi Pan

Masters Theses & Specialist Projects

Feature selection method is becoming an essential procedure in data preprocessing step. The feature selection problem can affect the efficiency and accuracy of classification models. Therefore, it also relates to whether a classification model can have a reliable performance. In this study, we compared an original feature selection method and a proposed frequency-based feature selection method with four classification models and three filter-based ranking techniques using a cancer dataset. The proposed method was implemented in WEKA which is an open source software. The performance is evaluated by two evaluation methods: Recall and Receiver Operating Characteristic (ROC). Finally, we found the ...


The Effects Of Carrier Ligands On Cisplatin Binding To Cysteine And Methionine, Adam C.R Smith Apr 2017

The Effects Of Carrier Ligands On Cisplatin Binding To Cysteine And Methionine, Adam C.R Smith

Masters Theses & Specialist Projects

We have reacted several derivatives of the anticancer drug cisplatin with N-acetyl-Lcysteine (N-AcCys) and N-acetyl-L-methionine (N-AcMet), which are two of the primary amino acid targets of platinum. NMR spectroscopy was used to monitor the reactions and determine the effect the different ligands would have on the platinum reactivity. Several of the platinum compounds were tested at pH of 4 and 7, and with platinum:amino acid ratios of 1:1, 2:1 and 1:2. Competition reactions between cysteine and methionine were done to confirm which would react with the platinum compound first. [Pt(dien)(NO3)]+ reacts faster with methionine ...


Detection And Quantitation Of Tetracycline Antibiotics In Agricultural Swine Wastes, Ali Jamal Abdulrheem Apr 2017

Detection And Quantitation Of Tetracycline Antibiotics In Agricultural Swine Wastes, Ali Jamal Abdulrheem

Masters Theses & Specialist Projects

The spread of tetracyclines through agricultural systems is causing the present bacteria to develop antibiotic resistance. The spread of this bacteria, as well as the tetracycline antibiotics in the environment is dangerous because these antibiotics pose health hazards for humans. The overuse of antibiotics, which are added to livestock feed, results in the antibiotics being released into the environment via animal feces. In this research, we have attempted to design an analytical method to isolate antibiotics from agricultural wastes with subsequent detection using liquid chromatography and mass spectrometry (LC-MS). The antibiotics investigated in this study were tetracycline, chlortetracycline, and oxytetracycline ...


Synthesis, Kinetic And Catalytic Studies Of Manganese Complexes With Corrole And Porphyrin Ligands, Haleh Jeddi Apr 2017

Synthesis, Kinetic And Catalytic Studies Of Manganese Complexes With Corrole And Porphyrin Ligands, Haleh Jeddi

Masters Theses & Specialist Projects

High-valent transition metal-oxo intermediates play a significant role in the catalytic cycle of the ubiquitous cytochrome P450 enzymes and in biomimetic catalytic systems. In this work, manganese(III) porphyrin and corrole systems (2) were synthesized and characterized by UV-vis absorbance and 1H-NMR, matching literaturereported spectroscopic data. Manganese(V)-oxo corroles (3) and a manganese(IV)-oxo porphyrin (4) were successfully generated by chemical oxidation using mchloroperoxybenzoic acid (m-CPBA), and their oxidation reactions with organic reductants were comparatively investigated. Results from single-turnover kinetic studies indicate that in the tris(pentafluorophenyl)corrole system (3a), the active oxidizing intermediate differs in different solvents ...


Visible-Light Generation Of High-Valent Metal-Oxo Intermediates And A Biomimetic Oxidation Catalyzed By Manganese Porphyrins With Iodobenzene Diacetate, Ka Wai Kwong Oct 2016

Visible-Light Generation Of High-Valent Metal-Oxo Intermediates And A Biomimetic Oxidation Catalyzed By Manganese Porphyrins With Iodobenzene Diacetate, Ka Wai Kwong

Masters Theses & Specialist Projects

High-valent iron-oxo intermediates play central roles as active oxidants in enzymatic and synthetic catalytic oxidations. Many transition metal catalysts are designed for biomimetic studies of the predominant oxidation catalysts in Nature, the cytochrome P450 enzymes.

In this work, a new photochemical method to generate high-valent iron-oxo porphyrin models was discovered. As controlled by the electronic nature of porphyrin ligands, iron(IV)-oxo porphyrin radical cations (Compound I model) and iron(IV)-oxo porphyrin derivatives (Compound II model) were produced. These observations indicate that the photochemical reactions involve a heterolytic cleavage of O-Br in precursors to give a putative iron(V ...


The Effects Of Sulfuric Acid Deposition On The Growth And Development Of Pond Breeding Salamanders In The Genus Ambystoma, Kenneth J. Anderson Oct 2016

The Effects Of Sulfuric Acid Deposition On The Growth And Development Of Pond Breeding Salamanders In The Genus Ambystoma, Kenneth J. Anderson

Masters Theses & Specialist Projects

In terrestrial habitats with a history of mining activity and previous or ongoing reclamation efforts, understanding the effects of acidification on the ecology of amphibians is an important part of the restoration process and the conservation of local amphibian populations. Pond-breeding amphibians spend much of their post metamorphic life history in direct contact with the soil in upland habitat adjacent to aquatic breeding sites. I reared recently metamorphosed marbled salamanders (Ambystoma opacum) to evaluate the role of soil acidity on determinants of fitness such as growth and survival. My results indicate that a substrate of pH 4 was lethal to ...


Development Of Dihydrochalcone Functionalized Gold Nanoparticles For Augmented Antineoplastic Activity, Jason N. Payne Oct 2016

Development Of Dihydrochalcone Functionalized Gold Nanoparticles For Augmented Antineoplastic Activity, Jason N. Payne

Masters Theses & Specialist Projects

Phloridzin, an antidiabetic and antineoplastic agent usually found in fruit trees, is a dihydrochalcone constituent that has a clinical/pharmaceutical significance as a sodiumglucose linked transport 2 (SGLT2) inhibitor. Phloridzin never experienced widespread clinical usage in the pharmaceutical market due to its side effects and poor bioavailability when compared to other antidiabetic therapeutics. The poor bioavailability is primarily attributed to the degradation of the glycosidic bond of the phloridzin, resulting in the formation of phloretin, the aglycone of phloridzin and glucose. While phloretin displays a reduced capacity of SGLT2 inhibition, this nutraceutical shows enhanced antineoplastic activity in comparison to phloridzin ...


Dispersion Of Ammonia From Concentrated Animal Feeding Operations, Austin D. Wardall Oct 2016

Dispersion Of Ammonia From Concentrated Animal Feeding Operations, Austin D. Wardall

Masters Theses & Specialist Projects

The purpose of this research is to investigate the dispersion of ammonia (NH3) from three Concentrated Animal Feeding Operations (CAFOs) in western Kentucky, as well as to investigate the Weather Research and Forecasting – Chemistry (WRF-Chem) model’s sensitivity response to initial NH3 concentrations under both wet conditions (significant precipitation) and dry conditions (no precipitation). As expected, pollutant concentrations generally were significantly higher near their points of origin and generally declined away from the sources. Contrary to expectations, ammonia tended to rise through the planetary boundary layer (PBL) regardless of atmospheric conditions. Results showed modeled NH3 pollution levels at the surface ...


Using Zinc Finger Proteins As A Diagnostic Tool For The Detection Of A Cancer Biomarker, Anu Kini Jul 2016

Using Zinc Finger Proteins As A Diagnostic Tool For The Detection Of A Cancer Biomarker, Anu Kini

Masters Theses & Specialist Projects

RASSF1A is a tumor suppressor gene which loses its function due to methylation of CpG islands on its promoter region. Detection of methylation leads to early diagnosis of cancer.

Zinc finger proteins are capable of detecting a specific DNA sequence and Methyl binding domain can bind to the methyl group on the CpG, using this idea mCpG SEER- Lac system makes use of a split protein, β-lactamase. Lac A attached to the ZFP and Lac B attached to the MBD protein. On binding to the DNA, the Lac A and Lac B come in close proximity with each other causing ...


Multiplexed Detection Of Double-Stranded Pathogenic Dna With Engineered Zinc Finger Proteins, Juhwa Kim Jul 2016

Multiplexed Detection Of Double-Stranded Pathogenic Dna With Engineered Zinc Finger Proteins, Juhwa Kim

Masters Theses & Specialist Projects

The development of a new technology for the detection of doublestranded (ds) DNA enables multiple biomedical applications including identifying multiple pathogens simultaneously. We previously employed colorimetric SEquence-Enabled Reassembly with TEM-1 β-lacatamase (SEER-LAC) to detect specific bacterial DNA sequence. SEER-Lac consists of the two inactive β-lactamase fragments which of each attached to a zinc finger protein (ZFP) would reassemble into an active full-length enzyme upon ZFPs binding to its target DNA. Here, we engineered two pairs of ZFPs which of each recognizes shiga toxin in E. coli O157 and staphylococcal enterotoxin B in Staphylococus Aureus, respectively. Biotin was simply conjugated to ...


Synthetic Investigation On The Biomimetic Metal-Catalyzed Sulfoxidations And Photochemical Generation Of A Highly Reactive Ruthenium(V)-Oxo Porphyrin, Weilong Luo Jul 2016

Synthetic Investigation On The Biomimetic Metal-Catalyzed Sulfoxidations And Photochemical Generation Of A Highly Reactive Ruthenium(V)-Oxo Porphyrin, Weilong Luo

Masters Theses & Specialist Projects

Catalytic oxidation plays a crucial role in current chemical and pharmaceutical industries which is also a leading technology for green chemical processes. In Nature, the ubiquitous cytochrome P450 enzymes can catalyze a wide variety of oxidation reactions with high efficiency and selectivity. Many transition metal catalysts are designed as the biomimetic model of cytochrome P450 enzymes. In this work, series of metalloporphyrins and metallocorroles have been successfully synthesized to investigate and develop catalytic selective oxidation of sulfides to sulfoxides.

Manganese(III) porphyrin complexes (2) and manganese(III) corrole complexes (6) with iodobenzene diacetate [PhI(OAc)2] as a mild oxygen ...


Evaluating The Influences Of Karst Hydrogeology On Freshwater Harmful Algal Blooms In Kentucky Lakes, Robert T. Schaefer Jul 2016

Evaluating The Influences Of Karst Hydrogeology On Freshwater Harmful Algal Blooms In Kentucky Lakes, Robert T. Schaefer

Masters Theses & Specialist Projects

A problem exists in Nolin River Lake and Rough River Lake in Kentucky, due to the increasing prevalence of cyanobacterial-based harmful algal blooms (CyanoHABs) and the threats they pose to local communities. These lakes were developed as artificial reservoirs from embankment. Further complicating the issue, the lakes are located within a heavily karstified region and there exists no plan or method currently for monitoring or managing CyanoHABs in a karst region with regard to groundwater inputs to the lake systems or their tributaries. A mixture of techniques and analysis methods was used to determine the best way to monitor and ...


Binding Of Oxaliplatin And Its Analogs With Dna Nucleotides At Variable Ph And Concentration Levels, Rippa Sehgal Apr 2016

Binding Of Oxaliplatin And Its Analogs With Dna Nucleotides At Variable Ph And Concentration Levels, Rippa Sehgal

Masters Theses & Specialist Projects

Oxaliplatin is one of the three FDA-approved platinum anticancer drugs and considered a third generation drug, discovered after the first generation drug cisplatin and second generation drug carboplatin. It is known to react with proteins and DNA nucleotides in the body. Reaction with DNA occurs primarily at guanosine residues and secondarily at adenine residues for oxaliplatin and other platinum drugs. We have previously studied oxaliplatin and an analog with additional steric hindrance in the amine ligand and found that the analog had different reactivity with methionine. Now, we have prepared oxaliplatin and its three analogs Pt(Me2dach)(ox), Pt(en ...


Expression And Purification Of Unlabelled And Isotopically Labelled Human Fibroblast Growth Factor-1 And Its Receptor Relevance In Cancer Research, Oluwadamilola Filani Oct 2015

Expression And Purification Of Unlabelled And Isotopically Labelled Human Fibroblast Growth Factor-1 And Its Receptor Relevance In Cancer Research, Oluwadamilola Filani

Masters Theses & Specialist Projects

Studies show that fibroblast growth factors (FGFs) control variety of cellular activities such as mitosis, cell differentiation, survival and angiogenesis. The FGF family consists of 23 different heparin-binding proteins. One of the most intensively studied members is human FGF-1 (hFGF-1) because of its critical role in the formation of blood vessels and cell proliferation in some types of cancer. The biological activities of FGFs are primarily mediated via interactions with fibroblast growth factor receptors (FGFR) and are a potent target in cancer. In this study, we report an efficient affinity column purification of hFGF-1 and the D2 domain of FGFR-2 ...


Evaluation Of Xanthan/Enzymatically Modified Guar Gum Mixtures In Oil-In-Water Emulsions, Pavan Kumar Chityala Jul 2015

Evaluation Of Xanthan/Enzymatically Modified Guar Gum Mixtures In Oil-In-Water Emulsions, Pavan Kumar Chityala

Masters Theses & Specialist Projects

Oil-in-water emulsions have wide range of applications in food industry because of their structure-forming properties, and as delivery systems of polyunsaturated fatty acids into foods. The thermodynamic instability of oil and water, and high susceptibility of unsaturated fatty acids to oxidation lead to physical and oxidative stability in oil-in-water emulsions. These instability processes are generally controlled by incorporating proteins and polysaccharides into oil-in-water emulsions. The objective of this study was to evaluate xanthan/enzymatically modified guar (XG/EMG) polysaccharides on the physical and oxidative stability of 2 wt% whey protein stabilized oil-in-water emulsions containing 20% v/v menhaden fish oil ...


An Apache Hadoop Framework For Large-Scale Peptide Identification, Harinivesh Donepudi Jul 2015

An Apache Hadoop Framework For Large-Scale Peptide Identification, Harinivesh Donepudi

Masters Theses & Specialist Projects

Peptide identification is an essential step in protein identification, and Peptide Spectrum Match (PSM) data set is huge, which is a time consuming process to work on a single machine. In a typical run of the peptide identification method, PSMs are positioned by a cross correlation, a statistical score, or a likelihood that the match between the trial and hypothetical is correct and unique. This process takes a long time to execute, and there is a demand for an increase in performance to handle large peptide data sets. Development of distributed frameworks are needed to reduce the processing time, but ...


Understanding The Molecular Level Interactions Of Cancer Inhibitor Imatinib With Human Fibroblast Growth Factor-1, Tulsi Modi May 2015

Understanding The Molecular Level Interactions Of Cancer Inhibitor Imatinib With Human Fibroblast Growth Factor-1, Tulsi Modi

Masters Theses & Specialist Projects

Fibroblast growth factors (FGFs) lack signal sequences, and are exported through endoplasmic reticulum (ER)-Golgi-independent non-classical routes. FGFs work as modulators of various cellular activities like mitosis, differentiation, survival etc. Among the FGF family, which comprises of 23 different heparin proteins, human FGF-1 (hFGF-1), a potent angiogenic factors are one of the targets in cancer inhibition, as they are involved in blood vessel formation in tissues. There has been intensive research directed at the development of drugs that could effectively inhibit angiogenesis. In this context, the purpose of this study is to fully understand the molecular principles essential to determine ...


Trophic Dynamic Interactions In A Temperate Karst River, Elizabeth Malloy Dec 2014

Trophic Dynamic Interactions In A Temperate Karst River, Elizabeth Malloy

Masters Theses & Specialist Projects

Surface streams in karst landscapes are often characterized by high nutrient levels due to incomplete filtration through series of innumerable, below-ground conduits. Seasonal growth of the filamentous alga, Cladophora, is typically associated with nutrient-rich waters. This research compared macroinvertebrate food web structure between riverine reaches with contrasting underlying karst topography, nutrient levels, and Cladophora cover during summer 2012 and autumn 2013. Recent work in these reaches found a high correlation between Cladophora cover and nutrient content, particularly nitrate. Four questions were addressed during this study: 1. Do longitudinal trends in algal and consumer δ13C values relate to decreased DIC availability ...


Single Step Synthesis Of Antibiotic Kanamycin Embedded Gold Nanoparticles For Efficient Antibacterial Activity, Shravan Gavva Aug 2013

Single Step Synthesis Of Antibiotic Kanamycin Embedded Gold Nanoparticles For Efficient Antibacterial Activity, Shravan Gavva

Masters Theses & Specialist Projects

Nanotechnology has become the most advanced type of drug delivery system within the last decade. This advancement shifted the focus on small carriers to increase the efficiency of the drugs. Among these, gold nanoparticles (GNPs) were found to have profound biomedical applications. In current research, kanamycin embedded GNPs were prepared in a single step, single phase, and bio-friendly (green synthesis) procedure. The synthesized Kanamycin-GNPs (Kan-GNPs) were spherical in shape and had a size range of 15 ± 3 nm. The chosen kanamycin is an aminoglycosidic antibiotic that is isolated from Streptomyces kanamyceticus. These special antibiotic GNPs are further characterized using several ...


Effects Of Xanthan/Locust Bean Gum Mixtures On The Physicochemical Properties And Oxidative Stability Of Whey Protein Stabilized Oil-In-Water Emulsions, Goutham Puli Aug 2013

Effects Of Xanthan/Locust Bean Gum Mixtures On The Physicochemical Properties And Oxidative Stability Of Whey Protein Stabilized Oil-In-Water Emulsions, Goutham Puli

Masters Theses & Specialist Projects

Scientific evidence shows that dietary intake of the omega-3 polyunsaturated fatty acids is beneficial to human health. Fish oil is a rich source of omega-3 fatty acids. However, fish oil with high levels of omega-3 PUFA is very susceptible to oxidative deterioration during storage. The objective of this study was to investigate the effect of xanthan gum (XG)-locust bean gum (LBG) mixtures on the physicochemical properties of whey protein isolate (WPI) stabilized oil-in-water (O/W) emulsions containing 20% v/v menhaden oil. The O/W emulsions containing XG/LBG mixtures were compared to emulsions with either XG or LBG ...


The Torsion Angle Of Random Walks, Mu He May 2013

The Torsion Angle Of Random Walks, Mu He

Masters Theses & Specialist Projects

In this thesis, we study the expected mean of the torsion angle of an n-step
equilateral random walk in 3D. We consider the random walk is generated within a confining sphere or without a confining sphere: given three consecutive vectors e1 , e2 , and e3 of the random walk then the vectors e1 and e2 define a plane and the vectors e2 and e3 define a second plane. The angle between the two planes is called the torsion angle of the three vectors. Algorithms are described to generate random walks which are used in ...


An Automatic Framework For Embryonic Localization Using Edges In A Scale Space, Zachary Bessinger May 2013

An Automatic Framework For Embryonic Localization Using Edges In A Scale Space, Zachary Bessinger

Masters Theses & Specialist Projects

Localization of Drosophila embryos in images is a fundamental step in an automatic computational system for the exploration of gene-gene interaction on Drosophila. Contour extraction of embryonic images is challenging due to many variations in embryonic images. In the thesis work, we develop a localization framework based on the analysis of connected components of edge pixels in a scale space. We propose criteria to select optimal scales for embryonic localization. Furthermore, we propose a scale mapping strategy to compress the range of a scale space in order to improve the efficiency of the localization framework. The effectiveness of the proposed ...


Contour Extraction Of Drosophila Embryos Using Active Contours In Scale Space, Soujanya Siddavaram Ananta Dec 2012

Contour Extraction Of Drosophila Embryos Using Active Contours In Scale Space, Soujanya Siddavaram Ananta

Masters Theses & Specialist Projects

Contour extraction of Drosophila embryos is an important step to build a computational system for pattern matching of embryonic images which aids in the discovery of genes. Automatic contour extraction of embryos is challenging due to several image variations such as size, shape, orientation and neigh- boring embryos such as touching and non-touching embryos. In this thesis, we introduce a framework for contour extraction based on the connected components in the gaussian scale space of an embryonic image. The active contour model is applied on the images to refine embryo contours. Data cleaning methods are applied to smooth the jaggy ...


Farmers' Markets In Kentucky: A Geospatial, Statistical, And Cultural Analysis, Elizabeth Ann Schmitz Dec 2010

Farmers' Markets In Kentucky: A Geospatial, Statistical, And Cultural Analysis, Elizabeth Ann Schmitz

Masters Theses & Specialist Projects

To assess what factors are driving the exponential growth of farmers’ markets in Kentucky, geospatial and statistical analysis of a database of 121 farmers’ markets was conducted. A statewide survey of market leaders and a case study of a single farmers’ market both identified reasons for growing support of farmers’ markets in Kentucky.

Market distribution, vendor levels, and gross sales were mapped against a backdrop of county urban classification, median household income, and education levels. Kruskal-Wallace analysis was used to identify if Kentucky’s rural, micropolitan, and metropolitan markets differ significantly in terms of their age, number of vendors, and ...