Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Series

PDF

Dairy Science

Institution
Keyword
Publication Year
Publication

Articles 1 - 30 of 834

Full-Text Articles in Life Sciences

Vascular Leak And Hypercytokinemia Associated With Severe Fever With Thrombocytopenia Syndrome Virus Infection In Mice, Jonna B. Westover, Brady T. Hickerson, Arnaud J. Van Wettere, Brett L. Hurst, Jacqueline P. Kurz, Ashley Dagley, Petra Wülfroth, Takashi Komeno, Yousuke Furuta, Thomas Steiner, Brian B. Gowen Sep 2019

Vascular Leak And Hypercytokinemia Associated With Severe Fever With Thrombocytopenia Syndrome Virus Infection In Mice, Jonna B. Westover, Brady T. Hickerson, Arnaud J. Van Wettere, Brett L. Hurst, Jacqueline P. Kurz, Ashley Dagley, Petra Wülfroth, Takashi Komeno, Yousuke Furuta, Thomas Steiner, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral hemorrhagic fever (VHF) endemic to China, South Korea, Japan, and Vietnam. Here we characterize the pathogenesis and natural history of disease in IFNAR-/- mice challenged with the HB29 strain of SFTS virus (SFTSV) and demonstrate hallmark features of VHF such as vascular leak and high concentrations of proinflammatory cytokines in blood and tissues. Treatment with FX06, a natural plasmin digest product of fibrin in clinical development as a treatment for vascular leak, reduced vascular permeability associated with SFTSV infection but did not significantly improve survival outcome. Further studies are needed ...


Function And Transcriptional Regulation Of Bovine Torc2 Gene In Adipocytes: Roles Of C/Ebpγ, Xbp1, Insm1 And Znf263, Rajwali Khan, Sayed Haidar Abbas Raza, Zainaguli Junjvlieke, Wang Xiaoyu, Matthew D. Garcia, Ibrahim Elsaeid Elnour, Wang Hongbao, Zan Linsen Sep 2019

Function And Transcriptional Regulation Of Bovine Torc2 Gene In Adipocytes: Roles Of C/Ebpγ, Xbp1, Insm1 And Znf263, Rajwali Khan, Sayed Haidar Abbas Raza, Zainaguli Junjvlieke, Wang Xiaoyu, Matthew D. Garcia, Ibrahim Elsaeid Elnour, Wang Hongbao, Zan Linsen

Animal, Dairy, and Veterinary Science Faculty Publications

The TORC2 gene is a member of the transducer of the regulated cyclic adenosine monophosphate (cAMP) response element binding protein gene family, which plays a key role in metabolism and adipogenesis. In the present study, we confirmed the role of TORC2 in bovine preadipocyte proliferation through cell cycle staining flow cytometry, cell counting assay, 5-ethynyl-20 -deoxyuridine staining (EdU), and mRNA and protein expression analysis of proliferation-related marker genes. In addition, Oil red O staining analysis, immunofluorescence of adiponectin, mRNA and protein level expression of lipid related marker genes confirmed the role of TORC2 in the regulation of bovine adipocyte differentiation ...


Basal Diet Determined Long-Term Composition Of The Gut Microbiome And Mouse Phenotype To A Greater Extent Than Fecal Microbiome Transfer From Lean Or Obese Human Donors, Daphne Michelle Rodriguez Jimenez, Abby D. Benninghoff, Niklas D.J. Aardema, Sumira Phatak, Korry J. Hintze Jul 2019

Basal Diet Determined Long-Term Composition Of The Gut Microbiome And Mouse Phenotype To A Greater Extent Than Fecal Microbiome Transfer From Lean Or Obese Human Donors, Daphne Michelle Rodriguez Jimenez, Abby D. Benninghoff, Niklas D.J. Aardema, Sumira Phatak, Korry J. Hintze

Animal, Dairy, and Veterinary Science Faculty Publications

The Western dietary pattern can alter the gut microbiome and cause obesity and metabolic disorders. To examine the interactions between diet, the microbiome, and obesity, we transplanted gut microbiota from lean or obese human donors into mice fed one of three diets for 22 weeks: (1) a control AIN93G diet; (2) the total Western diet (TWD), which mimics the American diet; or (3) a 45% high-fat diet-induced obesity (DIO) diet. We hypothesized that a fecal microbiome transfer (FMT) from obese donors would lead to an obese phenotype and aberrant glucose metabolism in recipient mice that would be exacerbated by consumption ...


Economic Contribution Of The Agricultural Sector To The Arkansas Economy In 2017, Leah English, Jennie Popp, Wayne Miller Jul 2019

Economic Contribution Of The Agricultural Sector To The Arkansas Economy In 2017, Leah English, Jennie Popp, Wayne Miller

Research Reports and Research Bulletins

Agriculture and associated agricultural activities are major contributors to the Arkansas economy. Agriculture is defined as the sum of agricultural production and processing activities, unless otherwise specified, and includes crop and animal production and processing, agricultural support industries, forestry and forest products, and textile goods. Agriculture contributes to the economy through direct agricultural production and value-added processing, and also leads to economic activity in other parts of the economy. This report is the eleventh in a series of reports examining agriculture’s economic contribution to the Arkansas economy. The total economic contribution of agriculture (direct, indirect, and induced effects) on ...


Young Dairymen, Mammoth Cave Chapter (Mss 662), Manuscripts & Folklife Archives Jun 2019

Young Dairymen, Mammoth Cave Chapter (Mss 662), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid and full-text scans of scrapbooks (Click on "Additional Files" below) for Manuscripts Collection 662. Scrapbooks (scanned copies only) and newsletters for the Young Dairymen, Mammoth Cave Chapter which was located in south central Kentucky and organized byH. Bland Dorris, Jr. The scrapbooks chiefly contain photographs of the group’s activities. Also included are the Chapter’s newsletters, 1982-1986.


Zika Virus Associated Pathology And Antigen Presence In The Testicle In The Absence Of Sexual Transmission During Subacute To Chronic Infection In A Mouse Model, Chad S. Clancy, Arnaud J. Van Wettere, John D. Morrey, Justin G. Julander Jun 2019

Zika Virus Associated Pathology And Antigen Presence In The Testicle In The Absence Of Sexual Transmission During Subacute To Chronic Infection In A Mouse Model, Chad S. Clancy, Arnaud J. Van Wettere, John D. Morrey, Justin G. Julander

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) is an arboviral infection that has been shown to be sexually transmitted. The study outlined herein aims to determine if accessory sex glands and epididymal epithelial cells are sources of viral persistence in subacute and chronic ZIKV infection, and if infection of these organs is important in sexual transmission during long-term (chronic) infection. Male interferon type I receptor knockout (Ifnar−/−) mice were challenged with ZIKV and reproductive tissues were harvested 14 and 35 days post infection (DPI) for inoculation studies and 14, 35 and 70 DPI for histopathology. Artificial insemination fluid derived from epididymal flush and seminal ...


Validation Of A Commercial Automated Body Condition Scoring System On A Commercial Dairy Farm, Israel L. Mullins, Carissa M. Truman, Magnus R. Campler, Jeffrey M. Bewley, Joao H. C. Costa May 2019

Validation Of A Commercial Automated Body Condition Scoring System On A Commercial Dairy Farm, Israel L. Mullins, Carissa M. Truman, Magnus R. Campler, Jeffrey M. Bewley, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

Body condition scoring (BCS) is the management practice of assessing body reserves of individual animals by visual or tactile estimation of subcutaneous fat and muscle. Both high and low BCS can negatively impact milk production, disease, and reproduction. Visual or tactile estimation of subcutaneous fat reserves in dairy cattle relies on their body shape or thickness of fat layers and muscle on key areas of the body. Although manual BCS has proven beneficial, consistent qualitative scoring can be difficult to implement. The desirable BCS range for dairy cows varies within lactation and should be monitored at multiple time points throughout ...


Incomplete Urethral Duplication Associated With A Dermoid Cyst Within A Vascular Hamartoma In A Female Dog, Chad S. Clancy, Gordon A. Hullinger, Arnaud J. Van Wettere May 2019

Incomplete Urethral Duplication Associated With A Dermoid Cyst Within A Vascular Hamartoma In A Female Dog, Chad S. Clancy, Gordon A. Hullinger, Arnaud J. Van Wettere

Animal, Dairy, and Veterinary Science Faculty Publications

A seven-year-old spayed female Labrador retriever presented for necropsy following an acute history of thrombocytopenia, anemia, leukocytosis and abdominal effusion. A 2 × 3 × 10 cm, cylindrical to tubular, mottled red-to-tan mass extended from the caudal pelvic cavity caudally and ventrally under the dermis along the caudal aspect of the left pelvic limb adjacent to the semimembranosus and semitendinosus musculature. Histologic examination of the mass revealed a singular central lumen lined by urothelium that multifocally transitioned into non-keratinizing, stratified squamous epithelium associated with few hair follicles and sweat glands. The lumen was surrounded by a dense collagenous stroma containing numerous, variably ...


Lack Of Selective Resistance Of Influenza A Virus In Presence Of Host-Targeted Antiviral, Uv-4b, Kelly L. Warfield, Kaitlyn R. Schaaf, Lisa Evans Dewald, Kevin B. Spurgers, Wei Wang, Eric Stavale, Michelle Mendenhall, Meghan H. Shilts, Timothy B. Stockwell, Dale L. Barnard, Urban Ramstedt, Suman R. Das May 2019

Lack Of Selective Resistance Of Influenza A Virus In Presence Of Host-Targeted Antiviral, Uv-4b, Kelly L. Warfield, Kaitlyn R. Schaaf, Lisa Evans Dewald, Kevin B. Spurgers, Wei Wang, Eric Stavale, Michelle Mendenhall, Meghan H. Shilts, Timothy B. Stockwell, Dale L. Barnard, Urban Ramstedt, Suman R. Das

Animal, Dairy, and Veterinary Science Faculty Publications

Development of antiviral drug resistance is a continuous concern for viruses with high mutation rates such as influenza. The use of antiviral drugs targeting host proteins required for viral replication is less likely to result in the selection of resistant viruses than treating with direct-acting antivirals. The iminosugar UV-4B is a host-targeted glucomimetic that inhibits endoplasmic reticulum α-glucosidase I and II enzymes resulting in improper glycosylation and misfolding of viral glycoproteins. UV-4B has broad-spectrum antiviral activity against diverse viruses including dengue and influenza. To examine the ability of influenza virus to develop resistance against UV-4B, mouse-adapted influenza virus was passaged ...


Genome To Phenome: Improving Animal Health, Production, And Well-Being – A New Usda Blueprint For Animal Genome Research 2018–2027, Caird Rexroad, Jeffrey Vallet, Lakshmi Kumar Matukumalli, James Reecy, Derek Bickhart, Harvey Blackburn, Mark Boggess, Hans Cheng, Archie Clutter, Noelle Cockett, Catherine Ernst, Janet E. Fulton, John Liu, Joan Lunney, Holly Neibergs, Et Al. May 2019

Genome To Phenome: Improving Animal Health, Production, And Well-Being – A New Usda Blueprint For Animal Genome Research 2018–2027, Caird Rexroad, Jeffrey Vallet, Lakshmi Kumar Matukumalli, James Reecy, Derek Bickhart, Harvey Blackburn, Mark Boggess, Hans Cheng, Archie Clutter, Noelle Cockett, Catherine Ernst, Janet E. Fulton, John Liu, Joan Lunney, Holly Neibergs, Et Al.

Animal, Dairy, and Veterinary Science Faculty Publications

In 2008, a consortium led by the Agricultural Research Service (ARS) and the National Institute for Food and Agriculture (NIFA) published the “Blueprint for USDA Efforts in Agricultural Animal Genomics 2008–2017,” which served as a guiding document for research and funding in animal genomics. In the decade that followed, many of the goals set forth in the blueprint were accomplished. However, several other goals require further research. In addition, new topics not covered in the original blueprint, which are the result of emerging technologies, require exploration. To develop a new, updated blueprint, ARS and NIFA, along with scientists in ...


Altered Gene Response To Aflatoxin B1 In The Spleens Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe Jr. Apr 2019

Altered Gene Response To Aflatoxin B1 In The Spleens Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe Jr.

Animal, Dairy, and Veterinary Science Faculty Publications

Susceptibility and/or resistance to aflatoxin B1 (AFB1) is a threshold trait governed principally by glutathione S transferase (GST)-mediated detoxification. In poultry, domesticated turkeys are highly sensitive to AFB1, most likely due to dysfunction in hepatic GSTs. In contrast, wild turkeys are comparatively resistant to aflatoxicosis due to the presence of functional hepatic GSTAs and other possible physiological and immunological interactions. The underlying genetic basis for the disparate GST function in turkeys is unknown as are the broader molecular interactions that control the systemic response. This study quantifies the effects of dietary AFB1 on gene ...


Efficacy Of A Ml336 Derivative Against Venezuelan And Eastern Equine Encephalitis Viruses, Colleen B. Jonsson, Xufeng Cao, Jasper Lee, Jon D. Gabbard, Yong-Kyu Chu, Elizabeth A. Fitzpatrick, Justin G. Julander, Dong-Hoon Chung, Jennifer Stabenow, Jennifer E. Golden Apr 2019

Efficacy Of A Ml336 Derivative Against Venezuelan And Eastern Equine Encephalitis Viruses, Colleen B. Jonsson, Xufeng Cao, Jasper Lee, Jon D. Gabbard, Yong-Kyu Chu, Elizabeth A. Fitzpatrick, Justin G. Julander, Dong-Hoon Chung, Jennifer Stabenow, Jennifer E. Golden

Animal, Dairy, and Veterinary Science Faculty Publications

Currently, there are no licensed human vaccines or antivirals for treatment of or prevention from infection with encephalitic alphaviruses. Because epidemics are sporadic and unpredictable, and endemic disease is common but rarely diagnosed, it is difficult to identify all populations requiring vaccination; thus, an effective post-exposure treatment method is needed to interrupt ongoing outbreaks. To address this public health need, we have continued development of ML336 to deliver a molecule with prophylactic and therapeutic potential that could be relevant for use in natural epidemics or deliberate release scenario for Venezuelan equine encephalitis virus (VEEV). We report findings from in vitro ...


Evaluating The Impact Of Feed Supplementation On Productive And Reproductive Efficiency In Smallholder Dairy Cattle In Arusha, Tanzania, Hayley Hall Apr 2019

Evaluating The Impact Of Feed Supplementation On Productive And Reproductive Efficiency In Smallholder Dairy Cattle In Arusha, Tanzania, Hayley Hall

Independent Study Project (ISP) Collection

This study was performed to determine if supplementing concentrates and/or minerals significantly affect productive and reproductive efficiency in smallholder dairy cattle in Arusha, Tanzania. The goal was to estimate the necessity and effectiveness of supplementing a forage diet on production and reproduction. Data was collected through interviews of small scale dairy cattle owners in nine regions within and around Arusha. Productive efficiency was measured by milk yield per cow per day. Reproductive efficiency was evaluated by the amount of time to heat resumption after calving and reproductive disease status. This was studied because determining the factors that improve the ...


Mapping Of Dynamic Transcriptome Changes Associated With Silica-Triggered Autoimmune Pathogenesis In The Lupus-Prone Nzbwf1 Mouse, Melissa A. Bates, Abby D. Benninghoff, Kristen N. Gilley, Andrij Holian, Jack R. Harkema, James J. Pestka Mar 2019

Mapping Of Dynamic Transcriptome Changes Associated With Silica-Triggered Autoimmune Pathogenesis In The Lupus-Prone Nzbwf1 Mouse, Melissa A. Bates, Abby D. Benninghoff, Kristen N. Gilley, Andrij Holian, Jack R. Harkema, James J. Pestka

Animal, Dairy, and Veterinary Science Faculty Publications

Crystalline silica (cSiO2) is a widely recognized environmental trigger of autoimmune disease. In the lupus-prone female NZBWF1 mouse, airway exposure to cSiO2 triggers pulmonary ectopic lymphoid neogenesis, systemic autoantibody elevation, and glomerulonephritis. Here we tested the hypothesis that upregulation of adaptive immune function genes in the lung precedes cSiO2-triggering of autoimmune disease in this model. The study include three groups of mice, as follows: (1) necropsied 1 d after a single intranasal instillation of 1 mg cSiO2 or vehicle, (2) necropsied 1 d after four weekly single instillations of 1 mg cSiO2 or vehicle ...


Fermentation Of Prebiotics In Whole Food Powders By Probiotic Bacteria Strains, Michaela Brubaker, Sumira Phatak Mar 2019

Fermentation Of Prebiotics In Whole Food Powders By Probiotic Bacteria Strains, Michaela Brubaker, Sumira Phatak

Research on Capitol Hill

Colorectal cancer (CRC) is the second leading cause of cancer death in the United States. Modification of the gut microbiome offers potential for CRC prevention.


Generation Of H7n9-Specific Human Polyclonal Antibodies From A Transchromosomic Goat (Caprine) System, Hua Wu, Zhiqiang Fan, Michelle Brandsrud, Qinggang Meng, Molly Bobbitt, Misha Regouski, Rusty Stott, Alexis Sweat, Jackelyn Crabtree, Robert J. Hogan, Ralph A. Tripp, Zhongde Wang, Irina A. Polejaeva, Eddie J. Sullivan Jan 2019

Generation Of H7n9-Specific Human Polyclonal Antibodies From A Transchromosomic Goat (Caprine) System, Hua Wu, Zhiqiang Fan, Michelle Brandsrud, Qinggang Meng, Molly Bobbitt, Misha Regouski, Rusty Stott, Alexis Sweat, Jackelyn Crabtree, Robert J. Hogan, Ralph A. Tripp, Zhongde Wang, Irina A. Polejaeva, Eddie J. Sullivan

Animal, Dairy, and Veterinary Science Faculty Publications

To address the unmet needs for human polyclonal antibodies both as therapeutics and diagnostic reagents, building upon our previously established transchromosomic (Tc) cattle platform, we report herein the development of a Tc goat system expressing human polyclonal antibodies in their sera. In the Tc goat system, a human artificial chromosome (HAC) comprising the entire human immunoglobulin (Ig) gene repertoire in the germline configuration was introduced into the genetic makeup of the domestic goat. We achieved this by transferring the HAC into goat fetal fibroblast cells followed by somatic cell nuclear transfer for Tc goat production. Gene and protein expression analyses ...


Human Polyclonal Antibodies Produced From Transchromosomal Bovine Provides Prophylactic And Therapeutic Protections Against Zika Virus Infection In Stat2 Ko Syrian Hamsters, Venkatraman Siddharthan, Jinxin Miao, Arnaud J. Van Wettere, Rong Li, Hua Wu, Eddie Sullivan, Jinan Jiao, Jay W. Hooper, David Safronetz, John D. Morrey, Justin G. Julander, Zhongde Wang Jan 2019

Human Polyclonal Antibodies Produced From Transchromosomal Bovine Provides Prophylactic And Therapeutic Protections Against Zika Virus Infection In Stat2 Ko Syrian Hamsters, Venkatraman Siddharthan, Jinxin Miao, Arnaud J. Van Wettere, Rong Li, Hua Wu, Eddie Sullivan, Jinan Jiao, Jay W. Hooper, David Safronetz, John D. Morrey, Justin G. Julander, Zhongde Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) infection can cause severe congenital diseases, such as microcephaly, ocular defects and arthrogryposis in fetuses, and Guillain–Barré syndrome in adults. Efficacious therapeutic treatments for infected patients, as well as prophylactic treatments to prevent new infections are needed for combating ZIKV infection. Here, we report that ZIKV-specific human polyclonal antibodies (SAB-155), elicited in transchromosomal bovine (TcB), provide significant protection from infection by ZIKV in STAT2 knockout (KO) golden Syrian hamsters both prophylactically and therapeutically. These antibodies also prevent testicular lesions in this hamster model. Our data indicate that antibody-mediated immunotherapy is effective in treating ZIKV infection. Because ...


Differential Transcriptome Responses To Aflatoxin B1 In The Cecal Tonsil Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe Jr. Jan 2019

Differential Transcriptome Responses To Aflatoxin B1 In The Cecal Tonsil Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe Jr.

Animal, Dairy, and Veterinary Science Faculty Publications

The nearly-ubiquitous food and feed-borne mycotoxin aflatoxin B1 (AFB1) is carcinogenic and mutagenic, posing a food safety threat to humans and animals. One of the most susceptible animal species known and thus a good model for characterizing toxicological pathways, is the domesticated turkey (DT), a condition likely due, at least in part, to deficient hepatic AFB1-detoxifying alpha-class glutathione S-transferases (GSTAs). Conversely, wild turkeys (Eastern wild, EW) are relatively resistant to the hepatotoxic, hepatocarcinogenic and immunosuppressive effects of AFB1 owing to functional gene expression and presence of functional hepatic GSTAs. This study was designed to compare ...


Jackrabbit Dairy And Food Review, Vikram V. Mistry Jan 2019

Jackrabbit Dairy And Food Review, Vikram V. Mistry

The Jackrabbit Dairy & Food Review

This is the Winter 2017- 2018 Jackrabbit Dairy and Food Review. It contains the following articles and information: Message from the Department Head, 2017 Graduates, David A. Thompson Endowment, New Faculty and Visitors, information on the Dairy Club and Food Science Club, Judging Activities, Student Accomplishments, Professional Activities of Faculty and Staff, Distinguished Alumni, Dairy Facilities, a list of Scholarships and Dairy and Food Science Students, information on Departmental Outreach and Research Highlights.


Using An Implantable Microchip For Measuring Body Temperature In Dairy Calves, Megan Woodrum, Melissa Cantor, Joao H. C. Costa Jan 2019

Using An Implantable Microchip For Measuring Body Temperature In Dairy Calves, Megan Woodrum, Melissa Cantor, Joao H. C. Costa

Posters-at-the-Capitol Presentations

Body temperature is frequently used as a method for determining if illness is present with fever detection. Taking rectal temperature or alternatively tympanic temperature can be time consuming and requires restraint of calves. Alternatives, such as implantable microchips that can be passively read using a radio frequency identification (RFID) scanner may allow for easier monitoring of body temperature.

This study aim was to validate an implantable microchip and to determine the best implant site.


Perceptions Of On-Farm Emergency Slaughter For Dairy Cows In British Columbia, Katherine E. Koralesky, David Fraser Jan 2019

Perceptions Of On-Farm Emergency Slaughter For Dairy Cows In British Columbia, Katherine E. Koralesky, David Fraser

Slaughter and Slaughtering Practices Collection

Some jurisdictions permit on-farm emergency slaughter (OFES) as one end-of-life option for dairy cows and other animals that cannot be transported humanely but are deemed fit for human consumption. Anecdotal reports suggest that OFES is controversial among dairy industry professionals, but to date their perceptions of OFES have not been studied systematically. Twentyfive individual interviews and 3 focus groups with 40 dairy producers, veterinarians, and other professionals in British Columbia, Canada, revealed positive and negative perceptions of OFES influenced by (1) individual values, (2) the perceived operational legitimacy of OFES, and (3) concern over social responsibility and public perception of ...


A Potent Lassa Virus Antiviral Targets An Arenavirus Virulence Determinant, Ikenna G. Madu, Megan Files, Dima N. Gharaibeh, Amy L. Moore, Kieh-Hoon Jung, Brian B. Gowen, Dongcheng Dai, Kevin F. Jones, Shanthakumar R. Tyavanagimatt, James R. Burgeson, Marcus J. Korth, Kristin M. Bedard, Shawn P. Iadonato, Sean M. Amberg Dec 2018

A Potent Lassa Virus Antiviral Targets An Arenavirus Virulence Determinant, Ikenna G. Madu, Megan Files, Dima N. Gharaibeh, Amy L. Moore, Kieh-Hoon Jung, Brian B. Gowen, Dongcheng Dai, Kevin F. Jones, Shanthakumar R. Tyavanagimatt, James R. Burgeson, Marcus J. Korth, Kristin M. Bedard, Shawn P. Iadonato, Sean M. Amberg

Animal, Dairy, and Veterinary Science Faculty Publications

Arenaviruses are a significant cause of hemorrhagic fever, an often-fatal disease for which there is no approved antiviral therapy. Lassa fever in particular generates high morbidity and mortality in West Africa, where the disease is endemic, and a recent outbreak in Nigeria was larger and more geographically diverse than usual. We are developing LHF-535, a small-molecule viral entry inhibitor that targets the arenavirus envelope glycoprotein, as a therapeutic candidate for Lassa fever and other hemorrhagic fevers of arenavirus origin. Using a lentiviral pseudotype infectivity assay, we determined that LHF-535 had sub-nanomolar potency against the viral envelope glycoproteins from all Lassa ...


Comparing Strategies For Selection Of Low-Density Snps For Imputation-Mediated Genomic Prediction In U.S. Holsteins, Jun He, Jiaqi Xu, Xiao-Lin Wu, Stewart Bauck, Jungjae Lee, Gota Morota, Stephen D. Kachman, Matthew L. Spangler Dec 2018

Comparing Strategies For Selection Of Low-Density Snps For Imputation-Mediated Genomic Prediction In U.S. Holsteins, Jun He, Jiaqi Xu, Xiao-Lin Wu, Stewart Bauck, Jungjae Lee, Gota Morota, Stephen D. Kachman, Matthew L. Spangler

Faculty Papers and Publications in Animal Science

SNP chips are commonly used for genotyping animals in genomic selection but strategies for selecting low-density (LD) SNPs for imputation-mediated genomic selection have not been addressed adequately. The main purpose of the present study was to compare the performance of eight LD (6K) SNP panels, each selected by a different strategy exploiting a combination of three major factors: evenly-spaced SNPs, increased minor allele frequencies, and SNP-trait associations either for single traits independently or for all the three traits jointly. The imputation accuracies from 6K to 80K SNP genotypes were between 96.2 and 98.2%. Genomic prediction accuracies obtained using ...


Management Of Cull Dairy Cows—Consensus Of An Expert Consultation In Canada, Jane Stojkov, G. Bowers, M. Draper, Todd Duffield, P. Duivenvoorden, M. Groleau, Deb Haupstein, R. Peters, Jane Pritchard, C. Radom, N. Sillett, W. Skippon, H. Trépanier, David Fraser Dec 2018

Management Of Cull Dairy Cows—Consensus Of An Expert Consultation In Canada, Jane Stojkov, G. Bowers, M. Draper, Todd Duffield, P. Duivenvoorden, M. Groleau, Deb Haupstein, R. Peters, Jane Pritchard, C. Radom, N. Sillett, W. Skippon, H. Trépanier, David Fraser

Farm Animal Welfare Collection

Many cull dairy cows enter the marketing system and travel to widely dispersed and specialized slaughter plants, and they may experience multiple handling events (e.g., loading, unloading, mixing), change of ownership among dealers, and feed and water deprivation during transport and at livestock markets. The objectives of this study were to describe the diverse management of cull dairy cows in Canada and establish consensus on ways to achieve improvements. A 2-day expert consultation meeting was convened, involving farmers, veterinarians, regulators, and experts in animal transport, livestock auction, and slaughter. The 15 participants, recruited from across Canada, discussed regional management ...


Pathogenesis Of Rift Valley Fever Virus Aerosol Infection In Stat2 Knockout Hamsters, Brady T. Hickerson, Jonna B. Westover, Arnaud J. Van Wettere, Johanna D. Rigas, Jinxin Miao, Bettina L. Conrad, Neil E. Motter, Zhongde Wang, Brian B. Gowen Nov 2018

Pathogenesis Of Rift Valley Fever Virus Aerosol Infection In Stat2 Knockout Hamsters, Brady T. Hickerson, Jonna B. Westover, Arnaud J. Van Wettere, Johanna D. Rigas, Jinxin Miao, Bettina L. Conrad, Neil E. Motter, Zhongde Wang, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

Rift Valley fever virus (RVFV) is an emerging pathogen capable of causing severe disease in livestock and humans and can be transmitted by multiple routes including aerosol exposure. Several animal models have been developed to gain insight into the pathogenesis associated with aerosolized RVFV infection, but work with these models is restricted to high containment biosafety level (BSL) laboratories limiting their use for antiviral and vaccine development studies. Here, we report on a new RVFV inhalation infection model in STAT2 KO hamsters exposed to aerosolized MP-12 vaccine virus by nose-only inhalation that enables a more accurate delivery and measurement of ...


Coitus-Free Sexual Transmission Of Zika Virus In A Mouse Model, Chad S. Clancy, Arnaud J. Van Wettere, John D. Morrey, Justin G. Julander Oct 2018

Coitus-Free Sexual Transmission Of Zika Virus In A Mouse Model, Chad S. Clancy, Arnaud J. Van Wettere, John D. Morrey, Justin G. Julander

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) is an arboviral infection that may be sexually transmitted. The present study aims to determine if accessory sex glands are a potential source of infectious virus and important in sexual transmission. Male interferon type I receptor knockout (Ifnar−/−) mice were challenged subcutaneously with a Puerto Rican ZIKV isolate. Reproductive tissues were harvested seven days after viral challenge and artificial insemination fluid derived from epididymis or homogenized accessory sex glands (seminal plasma) was obtained. Naïve interferon type I and II receptor knockout (AG129) females were pretreated with progesterone, and inoculated intravaginally with either epididymal flush or seminal plasma ...


James Roger Thomas, B. 1950 And Doris (Carson) Thomas (Sc 3273), Manuscripts & Folklife Archives Oct 2018

James Roger Thomas, B. 1950 And Doris (Carson) Thomas (Sc 3273), Manuscripts & Folklife Archives

MSS Finding Aids

Finding aid only for Manuscripts Small Collection 3273. Speech of Roger and Doris Thomas, Smiths Grove, Kentucky, given at a dairymen's event. They describe the history and operation of their dairy farm and their family and community activities. The remarks were made in conjunction with their forthcoming participation in a competition as representatives of the group.


A Novel Cancer Syndrome Caused By Kcnq1-Deficiency In The Golden Syrian Hamster, Rong Li, Jinxin Miao, Alexandru-Flaviu Tabaran, M. Gerard O'Sullivan, Kyle J. Anderson, Patricia M. Scott, Zhongde Wang, Robert T. Cormier Oct 2018

A Novel Cancer Syndrome Caused By Kcnq1-Deficiency In The Golden Syrian Hamster, Rong Li, Jinxin Miao, Alexandru-Flaviu Tabaran, M. Gerard O'Sullivan, Kyle J. Anderson, Patricia M. Scott, Zhongde Wang, Robert T. Cormier

Animal, Dairy, and Veterinary Science Faculty Publications

BACKGROUND: The golden Syrian hamster is an emerging model organism. To optimize its use, our group has made the first genetically engineered hamsters. One of the first genes that we investigated is KCNQ1 which encodes for the KCNQ1 potassium channel and also has been implicated as a tumor suppressor gene.

MATERIALS AND METHODS: We generated KCNQ1 knockout (KO) hamsters by CRISPR/Cas9­-mediated gene targeting and investigated the effects of KCNQ1­-deficiency on tumorigenesis.

RESULTS: By 70 days of age seven of the eight homozygous KCNQ1 KOs used in this study began showing signs of distress, and on necropsy six ...


Intramammary Infusion Of Casein Hydrolysate For Involution Of Single Mastitic Mammary Quarters Elevating Cow-Level Somatic Cell Count, Justine Britten, Kerry A. Rood, David J. Wilson Aug 2018

Intramammary Infusion Of Casein Hydrolysate For Involution Of Single Mastitic Mammary Quarters Elevating Cow-Level Somatic Cell Count, Justine Britten, Kerry A. Rood, David J. Wilson

Animal, Dairy, and Veterinary Science Faculty Publications

Mastitis in a single quarter can cause high somatic cell counts (SCC), clinical mastitis, and death in dairy cows. Currently, management of these mastitic quarters presents a problem for the dairy industry. Casein hydrolysate (CH) is an intramammary (IMM) infusion treatment reported to induce mammary involution. The primary objectives of this study were to investigate whether IMM CH treatment of single high SCC quarters, followed by cessation of quarter milk production for the remainder of lactation, was effective in reducing cow–level SCC and whether that quarter resumed milk production following calving. Three treatment groups were used: CH, non-hydrolyzed casein ...


Early Events In Japanese Encephalitis Virus Infection: Viral Entry, Sang-Im Yun, Young-Min Lee Aug 2018

Early Events In Japanese Encephalitis Virus Infection: Viral Entry, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV), a mosquito-borne zoonotic flavivirus, is an enveloped positive-strand RNA virus that can cause a spectrum of clinical manifestations, ranging from mild febrile illness to severe neuroinvasive disease. Today, several killed and live vaccines are available in different parts of the globe for use in humans to prevent JEV-induced diseases, yet no antivirals are available to treat JEV-associated diseases. Despite the progress made in vaccine research and development, JEV is still a major public health problem in southern, eastern, and southeastern Asia, as well as northern Oceania, with the potential to become an emerging global pathogen. In ...