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Faculty Publications

2016

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Articles 1 - 30 of 77

Full-Text Articles in Life Sciences

Multilayered Structure Of Tension Wood Cell Walls In Salicaceae Sensu Lato And Its Taxonomic Significance, Barbara Ghislain, Eric-André Nicolini, Raïssa Romain, Julien Ruelle, Arata Yoshinaga, Mac H. Alford, Bruno Clair Dec 2016

Multilayered Structure Of Tension Wood Cell Walls In Salicaceae Sensu Lato And Its Taxonomic Significance, Barbara Ghislain, Eric-André Nicolini, Raïssa Romain, Julien Ruelle, Arata Yoshinaga, Mac H. Alford, Bruno Clair

Faculty Publications

Salicaceae have been enlarged to include a majority of the species formerly placed in the polyphyletic tropical Flacourtiaceae. Several studies have reported a peculiar and infrequently formed multilayered structure of tension wood in four of the tropical genera. Tension wood is a tissue produced by trees to restore their vertical orientation and most studies have focused on trees developing tension wood by means of cellulose-rich, gelatinous fibres, as in Populus and Salix (Salicaceae s.s.). This study aims to determine if the multilayered structure of tension wood is an anatomical characteristic common in other Salicaceae and, if so, how its ...


Tlr8 Couples Socs-1 And Restrains Tlr7-Mediated Antiviral Immunity, Exacerbating West Nile Virus Infection In Mice, Amber M. Paul, Dhiraj Acharya, Linda Le, Penghua Wang, Dobrivoje S. Stokic, A. Arturo Leis, Lena Alexopoulou, Terrence Town, Richard A. Flavell, Erol Fikrig, Fengwei Bai Dec 2016

Tlr8 Couples Socs-1 And Restrains Tlr7-Mediated Antiviral Immunity, Exacerbating West Nile Virus Infection In Mice, Amber M. Paul, Dhiraj Acharya, Linda Le, Penghua Wang, Dobrivoje S. Stokic, A. Arturo Leis, Lena Alexopoulou, Terrence Town, Richard A. Flavell, Erol Fikrig, Fengwei Bai

Faculty Publications

West Nile virus (WNV) is a neurotropic ssRNA flavivirus that can cause encephalitis, meningitis, and death in humans and mice. Human TLR7 and TLR8 and mouse TLR7 recognize viral ssRNA motifs and induce antiviral immunity. However, the role of mouse TLR8 in antiviral immunity is poorly understood. In this article, we report that TLR8-deficient (Tlr8−/−) mice were resistant to WNV infection compared with wild-type controls. Efficient WNV clearance and moderate susceptibility to WNV-mediated neuronal death in Tlr8−/− mice were attributed to overexpression of Tlr7and IFN-stimulated gene-56 expression, whereas reduced expression of the proapoptotic gene coding Bcl2-associated X protein was observed ...


Running Decreases Knee Intra-Articular Cytokine And Cartilage Oligomeric Matrix Concentrations: A Pilot Study, Robert D. Hyldahl, Alyssa Evans, Sunku Kwon, Sarah T. Ridge, Eric Robinson, J. Ty Hopkins, Matthew K. Seeley Dec 2016

Running Decreases Knee Intra-Articular Cytokine And Cartilage Oligomeric Matrix Concentrations: A Pilot Study, Robert D. Hyldahl, Alyssa Evans, Sunku Kwon, Sarah T. Ridge, Eric Robinson, J. Ty Hopkins, Matthew K. Seeley

Faculty Publications

Introduction Regular exercise protects against degenerative joint disorders, yet the mechanisms that underlie these benefits are poorly understood. Chronic, low-grade inflammation is widely implicated in the onset and progression of degenerative joint disease.

Purpose To examine the effect of running on knee intra-articular and circulating markers of inflammation and cartilage turnover in healthy men and women.

Methods Six recreational runners completed a running (30 min) and control (unloaded for 30 min) session in a counterbalanced order. Synovial fluid (SF) and serum samples were taken before and after each session. Cytokine concentration was measured in SF and serum using a multiplexed ...


Genome Sequence Of Mycobacterium Phage Waterfoul, Paige N. Jackson, Ella K. Embry, Christa O. Johnson, Jessica R. Douglas, J. Michael Sellers, William A. D'Angelo, Dmitri V. Mavrodi Nov 2016

Genome Sequence Of Mycobacterium Phage Waterfoul, Paige N. Jackson, Ella K. Embry, Christa O. Johnson, Jessica R. Douglas, J. Michael Sellers, William A. D'Angelo, Dmitri V. Mavrodi

Faculty Publications

Waterfoul is a new isolated temperate siphovirus of Mycobacterium smegmatis mc2155. It was identified as a member of the K5 cluster of Mycobacterium phages and has a 61,248-bp genome with 95 predicted genes."


Historical Fire In Longleaf Pine (Pinus Palustris) Forests Of South Mississippi And Its Relation To Land Use And Climate, Charles Raymond White, Grant L. Harley Nov 2016

Historical Fire In Longleaf Pine (Pinus Palustris) Forests Of South Mississippi And Its Relation To Land Use And Climate, Charles Raymond White, Grant L. Harley

Faculty Publications

We characterized historical fire regimes in Pinus palustris (longleaf pine) forests of southern Mississippi with regard to global and regional coupled climate systems (e.g., El Niño–Southern Oscillation) and past human activity. The composite fire chronology spanned 1756–2013 with 132 individual scars representing 89 separate fire events. The mean fire interval was 2.9 yr, and mean intervals were significantly different between identified time periods (e.g., settlement period vs. management period). Evidence of biannual fire activity (up to three fires occurring within a 12‐ to 15‐month period) was found coeval with a peak in livestock grazing ...


Pyruvate Oxidase Of Streptococcus Pneumoniae Contributes To Penumolysin Release, Joseph C. Bryant, Ridge C. Dabbs, Katie L. Oswalt, Lindsey R. Brown, Jason W. Rosch, Keun S. Seo, Janet R. Donaldson, Larry S. Mcdaniel, Justin A. Thornton Nov 2016

Pyruvate Oxidase Of Streptococcus Pneumoniae Contributes To Penumolysin Release, Joseph C. Bryant, Ridge C. Dabbs, Katie L. Oswalt, Lindsey R. Brown, Jason W. Rosch, Keun S. Seo, Janet R. Donaldson, Larry S. Mcdaniel, Justin A. Thornton

Faculty Publications

Background

Streptococcus pneumoniae is one of the leading causes of community acquired pneumonia and acute otitis media. Certain aspects of S. pneumoniae’s virulence are dependent upon expression and release of the protein toxin pneumolysin (PLY) and upon the activity of the peroxide-producing enzyme, pyruvate oxidase (SpxB). We investigated the possible synergy of these two proteins and identified that release of PLY is enhanced by expression of SpxB prior to stationary phase growth.

Results

Mutants lacking the <>spxB gene were defective in PLY release and complementation of spxB restored PLY release. This was demonstrated by cytotoxic effects of sterile filtered ...


Fluctuating Asymmetry In Two Common Freshwater Fishes As A Biological Indicator Of Urbanization And Environmental Stress Within The Middle Chattahoochee Watershed, William I. Lutterschmidt, Samantha L. Martin, Jacob F. Schaefer Nov 2016

Fluctuating Asymmetry In Two Common Freshwater Fishes As A Biological Indicator Of Urbanization And Environmental Stress Within The Middle Chattahoochee Watershed, William I. Lutterschmidt, Samantha L. Martin, Jacob F. Schaefer

Faculty Publications

Deviations in bilateral symmetry or fluctuating asymmetry of an organism may result under environmental stressors that reduce developmental homeostasis and stability. Anthropogenic stressors such as increased urbanization can negatively impact environmental quality of aquatic ecosystems. Researchers have stressed the value in finding easy, accurate and inexpensive methods for assessing potential stress within ecosystems. Here we use fluctuating asymmetry (FA) as a useful quantitative tool in assessing the environmental quality and potential urban-based stressors within eight creeks of the Bull and Upatoi Creeks Watershed within the larger watershed of the Middle Chattahoochee. Using Geographic Information System (GIS), we characterize land-use patterns ...


A Polyaniline-Based Sensor Of Nucleic Acids, Partha Sengupta, Jared Gloria, Marcus K. Parker, Alex S. Flynt Nov 2016

A Polyaniline-Based Sensor Of Nucleic Acids, Partha Sengupta, Jared Gloria, Marcus K. Parker, Alex S. Flynt

Faculty Publications

Detection of nucleic acids is at the center of diagnostic technologies used in research and the clinic. Standard approaches used in these technologies rely on enzymatic modification that can introduce bias and artifacts. A critical element of next generation detection platforms will be direct molecular sensing, thereby avoiding a need for amplification or labels. Advanced nanomaterials may provide the suitable chemical modalities to realize label-free sensors. Conjugated polymers are ideal for biological sensing, possessing properties compatible with biomolecules and exhibit high sensitivity to localized environmental changes. In this article, a method is presented for detecting nucleic acids using the electroconductive ...


Diversity As Opportunity: Insights From 600 Million Years Of Ahr Evolution, Rebeka R. Merson, Mark E. Hahn, Sibel I. Karchner Oct 2016

Diversity As Opportunity: Insights From 600 Million Years Of Ahr Evolution, Rebeka R. Merson, Mark E. Hahn, Sibel I. Karchner

Faculty Publications

The aryl hydrocarbon receptor (AHR) was for many years of interest only to pharmacologists and toxicologists. However, this protein has fundamental roles in biology that are being revealed through studies in diverse animal species. The AHR is an ancient protein. AHR homologs exist in most major groups of modern bilaterian animals, including deuterostomes (chordates, hemichordates, echinoderms) and the two major clades of protostome invertebrates [ecdysozoans (e.g. arthropods and nematodes) and lophotrochozoans (e.g. molluscs and annelids)]. AHR homologs also have been identified in cnidarians such as the sea anemone Nematostella and in the genome of Trichoplax, a placozoan. Bilaterians ...


A Snapshot Of The Microbiome Of Ambylomma Tuberculatum Ticks Infesting The Gopher Tortoise, An Endangered Species, Khemraj Budachetri, Daniel Lyle Gaillard, Jaclyn Bo Williams, Nabanita Mukherjee, Shahid Karim Oct 2016

A Snapshot Of The Microbiome Of Ambylomma Tuberculatum Ticks Infesting The Gopher Tortoise, An Endangered Species, Khemraj Budachetri, Daniel Lyle Gaillard, Jaclyn Bo Williams, Nabanita Mukherjee, Shahid Karim

Faculty Publications

The gopher tortoise tick, Amblyomma tuberculatum, has a unique relationship with the gopher tortoise, Gopherus polyphemus, found in sandy habitats across the southeastern United States. We aimed to understand the overall bacterial community associated with A. tuberculatum while also focusing on spotted fever group Rickettsia. These tortoises in the Southern Mississippi region are a federally threatened species; therefore, we have carefully trapped the tortoises and removed the species-specific ticks attached to them. Genomic DNA was extracted from individual ticks and used to explore overall bacterial load using pyrosequencing of bacterial 16S rRNA on 454-sequencing platform. The spotted fever group of ...


Biomechanical Analysis Of Gait Termination In 11–17 Year Old Youth At Preferred And Fast Walking Speeds, Sarah T. Ridge, John Henley, Kurt Manal, Freeman Miller, James G. Richards Oct 2016

Biomechanical Analysis Of Gait Termination In 11–17 Year Old Youth At Preferred And Fast Walking Speeds, Sarah T. Ridge, John Henley, Kurt Manal, Freeman Miller, James G. Richards

Faculty Publications

In populations where walking and/or stopping can be difficult, such as in children with cerebral palsy, the ability to quickly stop walking may be beyond the child’s capabilities. Gait termination may be improved with physical therapy. However, without a greater understanding of the mechanical requirements of this skill, treatment planning is difficult. The purpose of this study was to understand how healthy children successfully terminate gait in one step when walking quickly, which can be challenging even for healthy children. Lower extremity kinematic and kinetic data were collected from 15 youth as they performed walking, planned, and unplanned ...


Nitrogen Loading Leads To Increased Carbon Accretion In Both Invaded And Uninvaded Coastal Wetlands, Jason P. Martina, William S. Currie, Deborah E. Goldberg, Kenneth J. Elgersma Sep 2016

Nitrogen Loading Leads To Increased Carbon Accretion In Both Invaded And Uninvaded Coastal Wetlands, Jason P. Martina, William S. Currie, Deborah E. Goldberg, Kenneth J. Elgersma

Faculty Publications

Gaining a better understanding of carbon (C) dynamics across the terrestrial and aquatic landscapes has become a major research initiative in ecosystem ecology. Wetlands store a large portion of the global soil C, but are also highly dynamic ecosystems in terms of hydrology and N cycling, and are one of the most invaded habitats worldwide. The interactions between these factors are likely to determine wetland C cycling, and specifically C accretion rates. We investigated these interactions using MONDRIAN, an individual-based model simulating plant growth and competition and linking these processes to N and C cycling. We simulated the effects of ...


Environmental Conditions Of 2 River Drainages Into The Northern Gulf Of Mexico During Successful Hatching Of Alabama Shad (Alosa Alabamae), Paul F. Mickle, Jacob F. Schaefer, Susan B. Adams, Brian R. Kreiser, William T. Slack Sep 2016

Environmental Conditions Of 2 River Drainages Into The Northern Gulf Of Mexico During Successful Hatching Of Alabama Shad (Alosa Alabamae), Paul F. Mickle, Jacob F. Schaefer, Susan B. Adams, Brian R. Kreiser, William T. Slack

Faculty Publications

In recent years, the Alabama shad (Alosa alabamae) has experienced dramatic declines and extirpations from portions of its native range. Habitat degradation and barriers to migration are considered contributing factors to contraction in the distributional range this species. To identify conditions during successful spawning, river temperatures and discharges in 2 drainages of the northern Gulf of Mexico (the Apalachicola and Pascagoula rivers) were characterized during successful hatching “windows.” Sampling during 2005–2009 yielded 400 juvenile Alabama shad of which 261 were aged from counts of rings on sagittal otoliths. Results from logistic regression revealed that successful spawning coincided with increases ...


Condition Of Larval Red Snapper (Lutjanus Campechanus) Relative To Environmental Variability And The Deepwater Horizon Oil Spill, Frank J. Hernandez Jr., J.E. Filbrun, J. Fang, John Timothy Ransom Sep 2016

Condition Of Larval Red Snapper (Lutjanus Campechanus) Relative To Environmental Variability And The Deepwater Horizon Oil Spill, Frank J. Hernandez Jr., J.E. Filbrun, J. Fang, John Timothy Ransom

Faculty Publications

The Deepwater Horizon oil spill(DWHOS)spatially and temporally overlapped with the spawning of many fish species, including Red Snapper, one of the most economically important reef fish in the Gulf of Mexico. To investigate potential impacts of the DWHOS on larval Red Snapper, data from a long-term ichthyoplankton survey off the coast of Alabama were used to examine: (1)larval abundances among pre-impact (2007–2009), impact(2010), and post-impact (2011, 2013) periods; (2) proxies for larval condition (size-adjusted morphometric relationships and dry weight) among the same periods; and (3) the effects of background environmental variation on larval condition. We ...


The Mitochondrial Cytochrome Oxidase Subunit I Gene Occurs On A Minichromosome With Extensive Heteroplasmy In Two Species Of Chewing Lice, Geomydoecus Aurei And Thomomydoecus Minor, Lucas L. Pietan, Theresa A. Spradling, James W. Demastes Sep 2016

The Mitochondrial Cytochrome Oxidase Subunit I Gene Occurs On A Minichromosome With Extensive Heteroplasmy In Two Species Of Chewing Lice, Geomydoecus Aurei And Thomomydoecus Minor, Lucas L. Pietan, Theresa A. Spradling, James W. Demastes

Faculty Publications

In animals, mitochondrial DNA (mtDNA) typically occurs as a single circular chromosome with 13 protein-coding genes and 22 tRNA genes. The various species of lice examined previously, however, have shown mitochondrial genome rearrangements with a range of chromosome sizes and numbers. Our research demonstrates that the mitochondrial genomes of two species of chewing lice found on pocket gophers, Geomydoecus aurei and Thomomydoecus minor, are fragmented with the 1,536 base-pair (bp) cytochrome-oxidase subunit I (cox1) gene occurring as the only protein-coding gene on a 1,916–1,964 bp minicircular chromosome in the two species, respectively. The cox1 gene of ...


Epstein-Barr Viral Micrornas Target Caspase 3, Cecelia Harold, Diana Cox, Kasandra J. Riley Aug 2016

Epstein-Barr Viral Micrornas Target Caspase 3, Cecelia Harold, Diana Cox, Kasandra J. Riley

Faculty Publications

The Epstein-Barr virus (EBV) is a ubiquitous herpesvirus that transforms B cells and causes several malignancies including Burkitt’s lymphoma. EBV differentially expresses at least 49 mature microRNAs (miRNAs) during latency in various infected epithelial and B cells. Recent high-throughput studies and functional assays have begun to reveal the function of the EBV miRNAs suggesting roles in latency, cell cycle control, and apoptosis. In particular, the central executioner of apoptosis, Caspase 3 (CASP3), was proposed as a target of select EBV miRNAs. However, whether CASP3 is truly a target of EBV miRNAs, and if so, which specific miRNAs target CASP3 ...


An Ultrasensitive Electrogenerated Chemiluminescence-Based Immunoassay For Specific Detection Of Zika Virus, Dhiraj Acharya, Pradip Bastola, Linda Le, Amber M. Paul, Estefania Fernandez, Michael S. Diamond, Wujian Miao, Fengwei Bai Aug 2016

An Ultrasensitive Electrogenerated Chemiluminescence-Based Immunoassay For Specific Detection Of Zika Virus, Dhiraj Acharya, Pradip Bastola, Linda Le, Amber M. Paul, Estefania Fernandez, Michael S. Diamond, Wujian Miao, Fengwei Bai

Faculty Publications

Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. As such, highly sensitive, specific, and cost-effective diagnostic methods are urgently needed. Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. We loaded polystyrene beads (PSB) with a large number of ECL labels and conjugated them with anti-ZIKV monoclonal antibodies to generate anti-ZIKV-PSBs. These anti-ZIKV-PSBs efficiently captured ZIKV in solution forming ZIKV-anti-ZIKV-PSB complexes, which were subjected to measurement of ECL intensity after further magnetic beads separation. Our results show that the ...


Integrative Approach For Inference Of Gene Regulatory Networks Using Lasso-Based Random Featuring And Application To Psychiatric Disorders, Dongchul Kim, Mingon Kang, Ashis Biswas, Chunyu Liu Aug 2016

Integrative Approach For Inference Of Gene Regulatory Networks Using Lasso-Based Random Featuring And Application To Psychiatric Disorders, Dongchul Kim, Mingon Kang, Ashis Biswas, Chunyu Liu

Faculty Publications

Background Inferring gene regulatory networks is one of the most interesting research areas in the systems biology. Many inference methods have been developed by using a variety of computational models and approaches. However, there are two issues to solve. First, depending on the structural or computational model of inference method, the results tend to be inconsistent due to innately different advantages and limitations of the methods. Therefore the combination of dissimilar approaches is demanded as an alternative way in order to overcome the limitations of standalone methods through complementary integration. Second, sparse linear regression that is penalized by the regularization ...


Virulence Factors Of Aeromonas Hydrophila: In The Wake Of Reclassification, Cody R. Rasmussen-Ivey, Maria J. Figueras, Donald Mcgarey, Mark R. Liles Aug 2016

Virulence Factors Of Aeromonas Hydrophila: In The Wake Of Reclassification, Cody R. Rasmussen-Ivey, Maria J. Figueras, Donald Mcgarey, Mark R. Liles

Faculty Publications

The ubiquitous “jack-of-all-trades,” Aeromonas hydrophila, is a freshwater, Gram-negative bacterial pathogen under revision in regard to its phylogenetic and functional affiliation with other aeromonads. While virulence factors are expectedly diverse across A. hydrophila strains and closely related species, our mechanistic knowledge of the vast majority of these factors is based on the molecular characterization of the strains A. hydrophila AH-3 and SSU, which were reclassified as A. piscicola AH-3 in 2009 and A. dhakensis SSU in 2013. Individually, these reclassifications raise important questions involving the applicability of previous research on A. hydrophila virulence mechanisms; however, this issue is exacerbated by ...


Functional Diversity Of Small And Large Trees Along Secondary Succession In A Tropical Dry Forest, Lucía Sanaphre-Villanueva, Juan Manuel Dupuy, José Luis Andrade, Casandra Reyes-García, Horacio Paz, Paula C. Jackson Jul 2016

Functional Diversity Of Small And Large Trees Along Secondary Succession In A Tropical Dry Forest, Lucía Sanaphre-Villanueva, Juan Manuel Dupuy, José Luis Andrade, Casandra Reyes-García, Horacio Paz, Paula C. Jackson

Faculty Publications

Functional Diversity is considered an important driver of community assembly in environmental and successional gradients. To understand tree assembly processes in a semideciduous tropical forest, we analyzed the variation of Functional Richness (FRic), Functional Divergence (FDiv), and Functional Evenness (FEve) of small vs. large trees in relation to fallow age after slash-and-burn agriculture and topographical position (flat sites vs. hills). FRic of small trees was lower than null model predicted values across the successional gradient, and decreased unexpectedly in older successional ages. FRic of large trees was higher than null model predictions early in succession and lower in late-successional stands ...


Identification Of Preferred Dna-Binding Sites For The Thermus Thermophilus Transcriptional Regulator Sbtr By The Combinatorial Approach Repsa, Michael W. Van Dyke, Matthew D. Beyer, Emily Clay, Jonathan L. Mcmurry Jul 2016

Identification Of Preferred Dna-Binding Sites For The Thermus Thermophilus Transcriptional Regulator Sbtr By The Combinatorial Approach Repsa, Michael W. Van Dyke, Matthew D. Beyer, Emily Clay, Jonathan L. Mcmurry

Faculty Publications

One of the first steps towards elucidating the biological function of a putative transcriptional regulator is to ascertain its preferred DNA-binding sequences. This may be rapidly and effectively achieved through the application of a combinatorial approach, one involving very large numbers of randomized oligonucleotides and reiterative selection and amplification steps to enrich for high-affinity nucleic acid-binding sequences. Previously, we had developed the novel combinatorial approach Restriction Endonuclease Protection, Selection and Amplification (REPSA), which relies not on the physical separation of ligand-nucleic acid complexes but instead selects on the basis of ligand-dependent inhibition of enzymatic template inactivation, specifically cleavage by type ...


Quantifying The Top-Down Effects Of Grazers On A Rocky Shore: Selective Grazing And The Potential For Competition, Diana Lascala-Gruenewald, Luke Miller, Matthew Bracken, Bengt Allen, Mark Denny Jul 2016

Quantifying The Top-Down Effects Of Grazers On A Rocky Shore: Selective Grazing And The Potential For Competition, Diana Lascala-Gruenewald, Luke Miller, Matthew Bracken, Bengt Allen, Mark Denny

Faculty Publications

The effect of grazers on the diversity, distribution, and composition of their principal food source has rarely been described for the high intertidal zone of rocky shores, a model system for studying the potential effects of climate change. Along rocky, wave-swept shores in central California, the microphytobenthos (MPB) supports diverse assemblages of limpets and littorine snails, which, at current benign temperatures, could potentially partition food resources in a complementary fashion, thereby enhancing secondary productivity. Two limpet species in particular, Lottia scabra and L. austrodigitalis, may partition components of the MPB, and are likely to affect the composition of the MPB ...


The Effect Of Post-Resistance Exercise Amino Acids On Plasma Mcp-1 And Ccr2 Expression, Adam J. Wells, Jay R. Hoffman, Adam R. Jajtner, Alyssa N. Varanoske Jul 2016

The Effect Of Post-Resistance Exercise Amino Acids On Plasma Mcp-1 And Ccr2 Expression, Adam J. Wells, Jay R. Hoffman, Adam R. Jajtner, Alyssa N. Varanoske

Faculty Publications

The recruitment and infiltration of classical monocytes into damaged muscle is critical for optimal tissue remodeling. This study examined the effects of an amino acid supplement on classical monocyte recruitment following an acute bout of lower body resistance exercise. Ten resistance-trained men (24.7 ˘ 3.4 years; 90.1 ˘ 11.3 kg; 176.0 ˘ 4.9 cm) ingested supplement (SUPP) or placebo (PL) immediately post-exercise in a randomized, cross-over design. Blood samples were obtained at baseline (BL), immediately (IP), 30-min (30P), 1-h (1H), 2-h (2H), and 5-h (5H) post-exercise to assess plasma concentrations of monocyte chemoattractant protein 1 (MCP-1), myoglobin ...


Comparison Of High-Intensity Vs. High-Volume Resistance Training On The Bdnf Response To Exercise, David D. Church, Jay R. Hoffman, Gerald T. Mangine, Adam R. Jajtner Jul 2016

Comparison Of High-Intensity Vs. High-Volume Resistance Training On The Bdnf Response To Exercise, David D. Church, Jay R. Hoffman, Gerald T. Mangine, Adam R. Jajtner

Faculty Publications

This study compared the acute and chronic response of circulating plasma brain-derived neurotrophic factor (BDNF) to high-intensity low-volume (HI) and low-intensity high volume (HV) resistance training. Twenty experienced resistance-trained men (23.5 ± 2.6 y, 1.79 ± 0.05 m, 75.7 ± 13.8 kg) volunteered for this study. Before the resistance training program (PRE), participants performed an acute bout of exercise using either the HI [3-5 reps; 90% of one repetition maximum (1RM)] or HV (10–12 reps; 70% 1RM) training paradigm. The acute exercise protocol was repeated after 7 wk of training (POST). Blood samples were obtained at ...


Structural Characterization Of Tick Cement Cones Collected From In Vivo And Artifical Membrane Blood-Fed Lone Star Ticks (Ambylomma Americanum), Rebekah Bullard, Paige Allen, Chien-Chung Chao, Jessica Douglas, Pradipta K. Das, Sarah E. Morgan, Wei-Mei Ching, Shahid Karim Jul 2016

Structural Characterization Of Tick Cement Cones Collected From In Vivo And Artifical Membrane Blood-Fed Lone Star Ticks (Ambylomma Americanum), Rebekah Bullard, Paige Allen, Chien-Chung Chao, Jessica Douglas, Pradipta K. Das, Sarah E. Morgan, Wei-Mei Ching, Shahid Karim

Faculty Publications

The Lone Star tick, Amblyomma americanum, is endemic to the southeastern United States and capable of transmitting pathogenic diseases and causing non-pathogenic conditions. To remain firmly attached to the host, the tick secretes a proteinaceous matrix termed the cement cone which hardens around the tick’s mouthparts to assist in the attachment of the tick as well as to protect the mouthparts from the host immune system. Cement cones collected from ticks on a host are commonly contaminated with host skin and hair making analysis of the cone difficult. To reduce the contamination found in the cement cone, we have ...


Traditional Vs. Flipped Library Instruction For The Life Sciences, Michael C. Goates, Megan Frost, Gregory M. Nelson Jun 2016

Traditional Vs. Flipped Library Instruction For The Life Sciences, Michael C. Goates, Megan Frost, Gregory M. Nelson

Faculty Publications

We compared search statement development between traditional lecture and flipped instruction sessions using two separate flipped models. Students in lecture sessions scored significantly higher on developing search statements than those in the flipped model 1 sessions. However, student scores were not significantly different between the lecture and the flipped model 2 sessions. Reasons for lower flipped-session scores may include a lack of student accountability, strong preference for a live demonstration, and disconnections between online tutorial content and in-class collaborative activities. Students in all sessions expressed a strong preference for pedagogies that incorporate elements from both lecture and flipped methodologies. Librarians ...


Local Versus Generalized Phenotypes In Two Sympatric Aurelia Species: Understanding Jellyfish Ecology Using Genetics And Morphometrics, Luciano M. Chiaverano, Keith Bayha, William M. Graham Jun 2016

Local Versus Generalized Phenotypes In Two Sympatric Aurelia Species: Understanding Jellyfish Ecology Using Genetics And Morphometrics, Luciano M. Chiaverano, Keith Bayha, William M. Graham

Faculty Publications

For individuals living in environmentally heterogeneous environments, a key component for adaptation and persistence is the extent of phenotypic differentiation in response to local environmental conditions. In order to determine the extent of environmentally induced morphological variation in a natural population distributed along environmental gradients, it is necessary to account for potential genetic differences contributing to morphological differentiation. In this study, we set out to quantify geographic morphological variation in the moon jellyfish Aurelia exposed at the extremes of a latitudinal environmental gradient in the Gulf of Mexico (GoM). We used morphological data based on 28 characters, and genetic data ...


Canned Vegetable And Fruit Consumption Is Associated With Changes In Nutrient Intake And Higher Diet Quality In Children And Adults: National Health And Nutrition Examination Survey 2001-2010, Marjorie Freedman, Victor Fulgoni Jun 2016

Canned Vegetable And Fruit Consumption Is Associated With Changes In Nutrient Intake And Higher Diet Quality In Children And Adults: National Health And Nutrition Examination Survey 2001-2010, Marjorie Freedman, Victor Fulgoni

Faculty Publications

Background Canned vegetables and fruit (CV+CF) are ubiquitous throughout the food supply. Yet information regarding their specific contribution to nutrient intake and health measures is lacking.Objective The objective of this study was to examine the association of CV+CF with nutrient intake, diet quality, anthropometric indicators of overweight/obesity, and blood pressure in a nationally representative population.Design and participants A secondary analysis of cross-sectional data from 17,344 children and 24,807 adults in the National Health and Nutrition Examination Survey 2001-2010 was conducted. A dataset was developed that distinguished CV+CF consumers from nonconsumers.Main outcome ...


Assessment Of Tick Antioxidant Responses To Exogenous Oxidative Stressors And Insight Into The Role Of Catalase In The Reproductive Fitness Of The Gulf Coast Tick, Amblyomma Maculatum, D. Kumar, K. Budachetri, V.C. Meyers, Shahid Karim Jun 2016

Assessment Of Tick Antioxidant Responses To Exogenous Oxidative Stressors And Insight Into The Role Of Catalase In The Reproductive Fitness Of The Gulf Coast Tick, Amblyomma Maculatum, D. Kumar, K. Budachetri, V.C. Meyers, Shahid Karim

Faculty Publications

As obligate blood‐sucking ectoparasites, to avoid tissue damage, ticks must neutralize the reactive oxygen species (ROS) generated from uptake and digestion of a bloodmeal. Consequently, ticks utilize a battery of antioxidant molecules, including catalase (CAT), an enzyme that converts hydrogen peroxide (H2O2) into water and oxygen. Here, we investigated the tick antioxidant machinery by exogenous injection of sublethal doses of H2O2 or paraquat. The relative transcript levels of selected Amblyomma maculatum antioxidant targets in tissues were determined by quantitative reverse transcriptase PCR following treatment. The results showed 2–16‐fold increases in target ...


Rickettsia Parkeri Colonization In Ambylomma Maculatum: The Role Of Superoxide Dismutases, Gary Crispell, Khemraj Budachetri, Shahid Karim May 2016

Rickettsia Parkeri Colonization In Ambylomma Maculatum: The Role Of Superoxide Dismutases, Gary Crispell, Khemraj Budachetri, Shahid Karim

Faculty Publications

Background

The Gulf Coast tick (Amblyomma maculatum) is an arthropod vector of Rickettsia parkeri, the causative agent of American boutonneuse fever and an infectious agent of public health significance. In this study, we evaluated the biological significance of the superoxide dismutases (SODs) of A. maculatum in hematophagy and R. parkeri colonization within the tick host.

Methods

An RNA interference approach was used to measure the functional roles of tick SODs (Cu/Zn-SOD and Mn-SOD) in R. parkeri colonization of the tick vector. Total microbial load, R. parkeri infection rate, and compensatory mechanisms by tick genes were examined using quantitative polymerase ...