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Biology Master’s Theses

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Full-Text Articles in Life Sciences

The Effects Of Rad1 And Rad10 On Mitochondrial Stability In The Saccharomyces Cerevisiae, Christina Seger Jul 2016

The Effects Of Rad1 And Rad10 On Mitochondrial Stability In The Saccharomyces Cerevisiae, Christina Seger

Biology Master’s Theses

The mitochondria have long been known as the powerhouse of the cell due to their essential role in oxidative phosphorylation to create energy. Mitochondria have their own genome separate from the nucleus, and may undergo mutations that lead to neuromuscular disease as well as account for some effects of aging. The effects and repair mechanisms of mutations to nuclear DNA have long been studied in order to map out the specific proteins and pathways involved. Nucleotide excision repair is a pathway involving a single-strand break which allows the template strand to be copied after removing the damaged bases. Rad1p and ...


Arginine Methylation And Enzymatic Activity Of Tblpn, A Lipin Homologue From T. Brucei, Andrew R. Bumstead Jul 2016

Arginine Methylation And Enzymatic Activity Of Tblpn, A Lipin Homologue From T. Brucei, Andrew R. Bumstead

Biology Master’s Theses

Trypanosoma brucei is a flagellated protozoan parasite responsible for African Trypanosomiasis. T. brucei is a deadly immune-evasive parasite which circulates the bloodstream of mammalian hosts and requires the Tsetse fly as a transmission vector. The parasite is capable of antigenic variation which allows it to change its glycoprotein coat in order to escape immune detection. Of great concern is the parasite’s growing resistance to various chemotherapeutic treatments which may allow for reemergence. Protein-arginine methyltransferases (PRMTs) are a group of proteins responsible for transferring methyl groups to arginine residues within proteins. Protein methylation causes epigenetic modification of histones or changes ...


Mitochondrial Dna Stability And Maintenance In Saccharomyces Cerevisiae, Nicole Ashman Jun 2016

Mitochondrial Dna Stability And Maintenance In Saccharomyces Cerevisiae, Nicole Ashman

Biology Master’s Theses

This research involved studying mitochondrial DNA stability using Saccharomyces cerevisiae as a model organism. Gene knockout strains focused on the genes FIS1, DNM1, CLU1 and RTG1. Both FIS1 and DNM1 are involved in mitochondrial fission. The CLU1 gene encodes a protein that may associate with the core complex of eukaryotic translation initiation factor 3 (eIF3) in Saccharomyces cerevisiae. The eIF3 plays a role in initiation of mRNA translation. The specific function of the Clu1p in this process is undefined. The gene knockout of CLU1 does not affect growth or translation initiation. The knockout does however cause defects in mitochondrial distribution ...


The Effects Of Ku70 On Mitochondrial Stability In The Saccharomyces Cerevisiae, Allyson Burkhart Jun 2016

The Effects Of Ku70 On Mitochondrial Stability In The Saccharomyces Cerevisiae, Allyson Burkhart

Biology Master’s Theses

The purpose of this experiment is to determine the role of KU70, a nuclear gene, in maintaining mitochondrial DNA in the model organism Saccharomyces cerevisiae, the budding yeast. The mitochondrion is an organelle in eukaryotes that produces much of the ATP used by a cell. ATP, or adenosine-triphosphate, is a molecule within a cell that provides energy for cellular functions via its high energy holding phosphate bonds. Mitochondria have their own genomes, separate from nuclear DNA, which encodes many proteins needed for cellular respiration. Mutations can occur in the mitochondria of humans that could result in decrease or loss of ...


The Influence Of Osmolytes On Nucleic Acid Helical Conformations, Pakinee Phromsiri May 2016

The Influence Of Osmolytes On Nucleic Acid Helical Conformations, Pakinee Phromsiri

Biology Master’s Theses

In the cell, nearly 40% of the volume is occupied by macromolecular crowding agents and smaller osmolytes that accumulate in response to environmental stresses. The effects of these cosolutes were observed on the transition of helical conformation from B-DNA to Z-DNA. Distinct from the familiar, right-handed B-DNA, Z-DNA is a left-handed double helical structure with its phosphodiester backbone arranged in a pronounced zig-zag pattern. This pattern, unique to Z-DNA is formed from alternating purine-pyrimidine sequences in the DNA. Due to the correlation between Z-DNA formation potential and regions of active transcription, Z-DNA is believed to serve a vital role in ...


Behavioral Phenotype Of Vang6 Mutant Drosophila Melanogaster Pertaining To The Olfactory System, Sarah Lynn Austin Jan 2016

Behavioral Phenotype Of Vang6 Mutant Drosophila Melanogaster Pertaining To The Olfactory System, Sarah Lynn Austin

Biology Master’s Theses

There are many proteins that aid in the development of the olfactory system, specifically Wnt pathway and planar cell polarity (PCP) pathway proteins. It has been shown that Vang6 mutant flies have distinct olfactory abnormalities, as do Wnt5 mutant flies. In addition, Drosophila melanogaster (Drosophila) Wnt5 mutants have an improper olfactory response compared to wildtype Drosophila. After using a T-maze to explore the behavioral tendencies of Vang6 mutant Drosophila and wildtype WT1118 flies, it was shown that there is no significance between wildtype and Vang6 mutant Drosophila selecting air (control component) or Carbon Dioxide (CO2) (test component).


Characterization Of Melanin-Concentrating Hormone Receptor Desensitization, Andrew E. Goodspeed Jun 2013

Characterization Of Melanin-Concentrating Hormone Receptor Desensitization, Andrew E. Goodspeed

Biology Master’s Theses

Melanin-concentrating hormone (MCH) receptor 1-knockout mice have limited incidence of diet-induced obesity. This makes the MCH signaling pathway a potential pharmacological target to fight human obesity. MCHR1 is a G-protein coupled receptor (GPCR) that activates multiple signaling pathways, including ERK phosphorylation. Overstimulation of GPCR signaling is a hallmark of many diseases. Likewise, inadequate desensitization of MCH signaling could potentiate the obese phenotype. GPCR desensitization typically involves agonist-induced internalization of activated receptors, and subsequent degradation or receptor recycling. The broad aim of this study was to determine the length and intensity of ERK phosphorylation and it's desensitization to MCHR1 activation ...


A Study Of The Sequestration Of Melanin-Concentrating Hormone Receptor-1 In Caveolae: A Potential Mode Of Cell Signaling Regulation By An Appetite-Stimulating Hormone, Lauren D. Field Aug 2012

A Study Of The Sequestration Of Melanin-Concentrating Hormone Receptor-1 In Caveolae: A Potential Mode Of Cell Signaling Regulation By An Appetite-Stimulating Hormone, Lauren D. Field

Biology Master’s Theses

The prevalence of obesity in the United States of America has increased over the last twenty years. This prevalence has led to an increase in the study of the hormones involved control of metabolism and satiety to further understand the factors involved in obesity. One of these hormones is melanin-concentrating hormone (MCH). Many of the studies of MCH focus on the brain, while little work is done on the peripheral tissues. In adipose cells stimulation with MCH causes a release of leptin through activation of melanin-concentrating hormone receptor-1, a G-protein coupled receptor. MCHR1 becomes desensitized after activation with MCH, but ...


Gastrointestinal (Gi) Motility Is Unaltered By Feeding In Zebrafish, Amanda Marie Diamond Aug 2012

Gastrointestinal (Gi) Motility Is Unaltered By Feeding In Zebrafish, Amanda Marie Diamond

Biology Master’s Theses

Gastrointestinal motility patterns in humans are not constant and respond to the luminal contents and nutrient status (Degen and Phillips, 1996). The zebrafish is a new model system for human GI motility and regulation of motility patterns appears to be controlled by enteric neurons and interstitial cells of Cajal (Huizinga et al., 1995, Farrugia et. al., 2003, Sanders et.al., 2006, Rich et. al., 2007). Although several research laboratories investigate GI motility in zebrafish larvae, no standard protocol for feeding exists and experiments may be performed on fasted or fed larvae. The goal of this study was to examine the ...


The Role Of Kitlb On Development Of Coordinated Muscular Contractions In The Zebrafish Gastrointestinal Tract, Brittany A. Heatherington Aug 2012

The Role Of Kitlb On Development Of Coordinated Muscular Contractions In The Zebrafish Gastrointestinal Tract, Brittany A. Heatherington

Biology Master’s Theses

Gastrointestinal (GI) motility is the spontaneous rhythmic contractions of smooth muscles that mix and propel the contents of the GI tract. Regulation of the complex muscular contractions is controlled by smooth muscles, interstitial cells of the Cajal (ICC) and enteric neurons. ICC act as pacemaker cells in the GI tract and set the frequency of spontaneous contractions. Altering ICC density results in uncoordinated GI muscular contractions. Our lab examines the role of

ICC in GI motility and is focused on mechanisms that regulate ICC growth and development. Expression of the Kit receptor tyrosine kinase is used to identify ICC. Kit ...


The Role Of Kitlb On Development Of Coordinated Muscular Contractions In The Zebrafish Gastrointestinal Tract, Brittany A. Heatherington Aug 2012

The Role Of Kitlb On Development Of Coordinated Muscular Contractions In The Zebrafish Gastrointestinal Tract, Brittany A. Heatherington

Biology Master’s Theses

Gastrointestinal (GI) motility is the spontaneous rhythmic contractions of smooth muscles that mix and propel the contents of the GI tract. Regulation of the complex muscular contractions is controlled by smooth muscles, interstitial cells of the Cajal (ICC) and enteric neurons. ICC act as pacemaker cells in the GI tract and set the frequency of spontaneous contractions. Altering ICC density results in uncoordinated GI muscular contractions. Our lab examines the role of ICC in GI motility and is focused on mechanisms that regulate ICC growth and development. Expression of the Kit receptor tyrosine kinase is used to identify ICC. Kit ...


Characterization Of In Vitro And In Vivo Enzymatic Activity Of Tblpn And Its Role In Phospholipids Biosynthesis In Trypanosoma Brucei, Dominic Nyamai Munini Jun 2012

Characterization Of In Vitro And In Vivo Enzymatic Activity Of Tblpn And Its Role In Phospholipids Biosynthesis In Trypanosoma Brucei, Dominic Nyamai Munini

Biology Master’s Theses

Trypanosoma brucei belongs to a group of parasitic protozoan called flagellates. It causes African sleeping sickness and is transmitted by tsetse fly of genus Glossina. African sleeping is a devastating vector-borne disease which threatens over 60 million people in 36 Sub-Saharan Africa. It causes over 70,000 deaths annually and is fatal unless treated. Reemergence of the disease in recent years has been a concern for scientists. There is concern on design of new drugs for the treatment of the disease because of the toxic effects of drugs designed around 50 years ago. Trypanosomes have great importance over the other ...


Characterization Of Tblpn In Trypanosoma Brucei, Alyssa S. Frainier May 2012

Characterization Of Tblpn In Trypanosoma Brucei, Alyssa S. Frainier

Biology Master’s Theses

Trypanosoma brucei, is a flagellated, unicellular, parasitic protozoan transmitted by the tsetse fly. It is the source of African sleeping sickness in humans. African sleeping sickness has two different stages, the bloodstream and central nervous system stages, each characterized by different symptoms. Problems with treatment result from severe side effects of the drugs used to treat African sleeping sickness. No vaccine is available due to high antigenic variation. T. brucei exists as two forms. The procyclic fly form relies on oxidative phosphorylation, expresses procyclin as its surface protein, and is morphologically long and slender. In contrast, the mammalian bloodstream form ...


An Investigation Of Melanin-Concentrating Hormone Receptor Internalization – Or Lack Thereof, Jay I. Moden Jr. Jan 2012

An Investigation Of Melanin-Concentrating Hormone Receptor Internalization – Or Lack Thereof, Jay I. Moden Jr.

Biology Master’s Theses

Melanin-concentrating hormone (MCH), a cyclic peptide hormone involved in energy homeostasis, is known to bind to two G protein-coupled receptors (GPCRs) in mammals. These receptors, melanin-concentrating hormone receptor 1 (MCHR1) and melanin-concentrating hormone receptor 2 (MCHR2), have been a popular target for MCH antagonists in an effort to fight the ongoing epidemic of obesity. In the presence of prolonged stimulus it is common for GPCRs to undergo rapid desensitization. However, the desensitization mechanisms of MCHR1 and MCHR2 are as yet poorly understood. This study aims to create epitope-tagged expression vectors to allow for the expression of MCHR1 and MCHR2 in ...


Red Lionfish (Pterois Volitans) Invade San Salvador, Bahamas: Early Population Characteristics, And Comparisons Of The Coral And Fish Communities On Shallow Patch Reefs In 2001 And 2007, Amanda K. Alexander Aug 2011

Red Lionfish (Pterois Volitans) Invade San Salvador, Bahamas: Early Population Characteristics, And Comparisons Of The Coral And Fish Communities On Shallow Patch Reefs In 2001 And 2007, Amanda K. Alexander

Biology Master’s Theses

Biological invaders are a leading contributor to global losses of biodiversity. A recent invader to the waters surrounding San Salvador, Bahamas, the red lionfish, Pterois volitans, was first reported in 2006; by 2009 they were common in waters 2 - 40 m deep around the island. Among the 5,078 fish observed on shallow patch reefs in 2007, only two were P. volitans; they were much more prevalent in deeper water along San Salvador's "wall." Captured P. volitans ranged in size from 19-32 cm, all longer than maturity length. Pallid goby ( Coryphopterus eidolon), black cap basslet ( Gramma melacara) and red ...


Expression Level Of Kita, Kitb, Kitla, And Kitlb In Zebrafish Gastrointestional Tract, Jennifer B. Strouse Jun 2011

Expression Level Of Kita, Kitb, Kitla, And Kitlb In Zebrafish Gastrointestional Tract, Jennifer B. Strouse

Biology Master’s Theses

Gastrointestinal (GI) motility is the muscular contractions that move intestinal contents in an anterograde (mouth to anus) direction and is necessary for nutrient absorption and elimination of waste. GI motility is highly coordinated and rhythmic contraction patterns. Interstitial cells of Cajal (ICC), enteric neurons, and smooth muscle cells all regulate GI motility. ICC function as pacemaker cells and determine contraction frequency. ICC growth and development is influenced by Kit, a tyrosine kinase receptor located on the plasma membrane of ICC. TMEM16A is a calcium activated chloride channel which contributes to the slow wave in the GI tract. Constipation, delayed gastric ...


Regulation Of Actin Dynamics By Melanin-Concentrating Hormone Potentiates Downstrean Signaling To Extracellular Signal-Regulated Kinase In Cultured Cells, Scott Michael Portwood Mar 2011

Regulation Of Actin Dynamics By Melanin-Concentrating Hormone Potentiates Downstrean Signaling To Extracellular Signal-Regulated Kinase In Cultured Cells, Scott Michael Portwood

Biology Master’s Theses

Melanin concentrating hormone (MCH) binds and activates two G protein-coupled receptors involved in the control of appetite and energy expenditure in mammals. MCH receptor-I interacts with two cytoskeleton-binding proteins in vitro. One of these proteins, periplakin, has been shown to contribute to the desensitization of MCH signaling events by displacing the interacting G protein. While periplakin is an actin- and intermediate filament-binding protein, MCH is not known to signal cytoskeletal rearrangements. These studies ask whether MCH receptors mediate actin cytoskeletal rearrangements in response to hormone binding. 3T3-L l pre-adipocytes, endogenously expressing MCH receptors, were treated with MCH for varying times ...


Defining The Limits And Mapping The Control Sequences Of The Gene, Enhancer Of Rudimentary, In Drosophila Melanogaster, Nicholas Rizzo Nov 2009

Defining The Limits And Mapping The Control Sequences Of The Gene, Enhancer Of Rudimentary, In Drosophila Melanogaster, Nicholas Rizzo

Biology Master’s Theses

The enhancer of rudimentary gene, e(r) is a highly conserved gene located on the X chromosome in Drosophila melanogaster. The gene encodes a transcriptional co-factor of 104 amino acids in length, and although the exact function is still unclear it has been shown to be involved in pyrimidine biosynthesis, oogenesis, and the cell cycle. Mutations in e(r) are characterized by low viability and decreased fertility. They have also been shown to enhance phenotypes caused by mutations in two genes involved in neurogenesis, notch and deltex, suggesting a possible role in neurogenesis. In the present study, transgenes of e ...


The Role Of The Mrx Complex And The Non-Homologous End Joining Dna Repair Pathway In Mitochondrial Genome Stability And Repair, Garry L. Coles Aug 2009

The Role Of The Mrx Complex And The Non-Homologous End Joining Dna Repair Pathway In Mitochondrial Genome Stability And Repair, Garry L. Coles

Biology Master’s Theses

Mitochondria are required for cellular respiration, which is essential in the production of ATP. Mitochondrial genome maintenance is necessary for the continued function of the mitochondrion. Deletions within the mitochondrial DNA (mtDNA) have been shown to be associated with a variety of human neuromuscular and age-related diseases. In this study we investigated the role of the MRX complex and the non-homologous end joining (NHEJ) DNA repair pathway in mitochondrial genome stability and repair. Specifically, we investigated the role of the MRX complex and the NHEJ pathway in the occurrence of spontaneous mitochondrial direct repeat-mediated deletions, nuclear direct repeat-mediated deletions, mitochondrial ...


Habitat Selection Of Shrubland Birds In The Great Lakes Plains Region Of New York State, Kristina A. Klees Jul 2008

Habitat Selection Of Shrubland Birds In The Great Lakes Plains Region Of New York State, Kristina A. Klees

Biology Master’s Theses

Over the course of the last century, shrubland habitat in the northeastern United States has declined due to farmland abandonment, deforestation, reforestation, human population growth and increased anthropogenic efforts to limit natural disturbances. In turn, these landscape alterations have caused a decline in the shrubland guild of birds in the northeastern United States, specifically the Great Lakes Plain Region. Declines have been so significant that wildlife managers must actively conserve existing shrublands and create new habitat to support shrubland birds. Thus, to offer suggestions for conservation and management of shrubland habitats and the birds that rely on them, I studied ...


Melanin-Concentrating Hormone Receptor-1 Is Enriched In Lipid Rafts And The Effects Of Lipid Raft Integrity On Receptor Signaling, Elizabeth Delorme-Axford Jul 2008

Melanin-Concentrating Hormone Receptor-1 Is Enriched In Lipid Rafts And The Effects Of Lipid Raft Integrity On Receptor Signaling, Elizabeth Delorme-Axford

Biology Master’s Theses

The melanin-concentrating hormone receptor-1 (MCHR-1) is a member of the G protein-coupled receptor (GPCR) superfamily, and functions in the regulation of food consumption and energy metabolism. MCHR-1 is expressed in neural, pancreatic, and fat tissue. Fat cells begin their journey as pre-adipocytes, culminating in the formation of mature adipocytes as differentiation occurs. Of interest to note, fat cells accumulate caveolae markers -caveolin-1 and cholesterol -during this process. Certain GPCRs and their associated downstream signaling molecules co-localize with caveolae membranes.

This thesis seeks to demonstrate that MCHR-1 is enriched in lipid rafts and that a possible consequence of this may be ...


The Role Of Clu1 In Maintaining Mitochondrial Genome Stability And Morphology In S. Cerevisiae, Christine E. Hochmuth Feb 2008

The Role Of Clu1 In Maintaining Mitochondrial Genome Stability And Morphology In S. Cerevisiae, Christine E. Hochmuth

Biology Master’s Theses

Mitochondrial genome maintenance is essential for the normal function of the cell. Mitochondrial DNA (mtDNA) is located in the matrix, where it is in close proximity to the electron transport chain, which is within the inner mitochondrial membrane. During oxidative phosphorylation, the electron transport chain produces reactive oxygen species (ROS) that may damage the DNA and contribute to mutations within the genome. Mutations in the mitochondrial genome have long been hypothesized as a contributor to diseases, especially those of the neuromuscular system. Mitochondrial mutations have also been linked to some types of cancer, programmed cell death, and aging in humans ...


A New Functional Zebrafish Gastrointestinal Motility Assay, Stacey Hess Aug 2007

A New Functional Zebrafish Gastrointestinal Motility Assay, Stacey Hess

Biology Master’s Theses

Gastrointestinal (GI) motility is the coordinated contractions of smooth muscles resulting in mixing and propulsion of material through the GI tract. GI motility is influenced by smooth muscle, enteric neurons, and Interstitial cells of Cajal (ICC). New model systems for GI motility are needed because regulation of motility is poorly understood, and the number of drugs which can assist in GI motility disorders is insufficient. The Kit receptor is required for ICC development and maintenance in mammalian model systems and in humans. Mutations in the Kit receptor are associated with GI motility disorders. The objective of these experiments was to ...


A Fundamental Investigation Into The Presence Of Interstitial Cells Of Cajal Within The Gastrointestinal Tract Of Dania Rerio, Scott Leddon Jul 2007

A Fundamental Investigation Into The Presence Of Interstitial Cells Of Cajal Within The Gastrointestinal Tract Of Dania Rerio, Scott Leddon

Biology Master’s Theses

Coordinated gastrointestinal (GI) motility results from the complex interactions between interstitial cells of Cajal (ICC), enteric neurons, and smooth muscle cells. Kit positive immunoreactivity has been extensively utilized as a marker for ICC; these cells function to generate rhythmic depolarization in the GI smooth muscle termed the electrical slow wave. Coordinated GI motility is regulated by the electrical slow wave. Directly lesioning ICC populations with neutralizing antibodies results in GI dysmotility and loss of the electrical slow wave. Furthermore, GI dysmotility symptoms and other human pathologies have been correlated with ICC deficiencies within the GI tract. Currently, there are few ...


Sediment Phosphorus Cycling In A Managed System Irondequoit Bay, N.Y., Daniel J. White Dec 2006

Sediment Phosphorus Cycling In A Managed System Irondequoit Bay, N.Y., Daniel J. White

Biology Master’s Theses

The current status of internal phosphorus loading was evaluated in Irondequoit Bay, NY, an embayment of Lake Ontario. Sediment core incubation experiments and phosphorus water column profiles were used to quantify the release rate of phosphorus from the deepwater sediments of Irondequoit Bay during summer periods of low hypolimnetic oxygen. The top 25 cm of sediment cores collected monthly from May 2004 through September 2004 were analyzed for total phosphorus, total manganese, total iron, and total aluminum. Phosphorus fractionation of the sediment cores was determined through sequential extraction. Phosphorus water column data collected during the summer of 2003 and 2004 ...


A Comparison Of Created And Natural Wetlands And The Effect Of Landscape Characteristics On Vegetation, Amphibian And Bird Variables In Freshwater Marshes Of Upstate New York, David W. Porter Aug 2006

A Comparison Of Created And Natural Wetlands And The Effect Of Landscape Characteristics On Vegetation, Amphibian And Bird Variables In Freshwater Marshes Of Upstate New York, David W. Porter

Biology Master’s Theses

Wetlands provide a number of ecological and social functions, including flood abatement, improvement of water quality, recharge ground water and support for a great diversity of flora and fauna. Despite their many functions and values, wetlands have not always been appreciated. Since the 1780s, it is estimated that 53% of wetland acreage in the United States has been lost due to draining, filling and the subsequent development of land for roads or farms.

In 1972, the Clean Water Act (CWA) was established to try to reverse declining wetland acreage in the U.S. According to Section 404 of the CW ...


Fat Stores, Plumage Morphs, And Sex Of Migrant White-Throated Sparrows, Brendan J. Mccabe Aug 2006

Fat Stores, Plumage Morphs, And Sex Of Migrant White-Throated Sparrows, Brendan J. Mccabe

Biology Master’s Theses

Abstract - A between-season comparison of lipid stores carried by migrant White-throated

Sparrows, as determined by deuterium dilution and visible fat class.

I compared qualitative and quantitative measures of fat stores for 144 migrant White-throated

Sparrows (Zonotrichia albicolis) in fall and spring. Qualitative measures were visible fat classes and a morphometric index (100 * mass / wind chord). I calculated lipid index (fat mass/fat-free dry mass) for the quantitative measure of fat stores using the deuterium dilution technique. Spring migrants had significantly higher fat class (2.0 ± 0.1 vs. 1.0 ± 0.2), lipid index (1.06 ± 0.03 vs. 0 ...


The Role Of Ilv5p Interacting Factors In Mitochondrial Dna Stability, Anthony J. Mirando Jun 2006

The Role Of Ilv5p Interacting Factors In Mitochondrial Dna Stability, Anthony J. Mirando

Biology Master’s Theses

The ease of manipulating yeast allows for advanced studies on the factors affecting the mitochondrial DNA mutation rates. The control mechanisms of the mitochondrial DNA mutation rate has been determined to involve the dual function protein, Ilv5p. The Ilv5p plays an integral role in the proper segregation of newly replicated mitochondrial DNA into daughter cells during cell division. The focus of this study is to find unknown factors involved in mitochondrial DNA stability. This study uses the Ilv5p to pull unknown factors out of the many genes that comprise the yeast genome. The identification of interacting factors of the Ilv5p ...


Evaluation Of Gyp7 Protein Ability To Coordinate And Regulate Mitochondrial Genomes Stability, Louis Didone Jun 2006

Evaluation Of Gyp7 Protein Ability To Coordinate And Regulate Mitochondrial Genomes Stability, Louis Didone

Biology Master’s Theses

Cellular creation of adenosine triphosphate, ATP, is essential for eukaryotic cells to function properly. The ATP molecule drives most of the biochemical and metabolic pathways of the cell. The cell's ATP is produced in the mitochondria. Mutations within the genome of the mitochondria will alter the cell's ability to generate A TP. Preliminary work has shown that loss of the Gyp 7p in Saccharomyces cerevisiae blocks the ability of mitochondria to properly function. The Gyp 7 gene was isolated using a technique called two-hybrid analysis with a known mitochondrial protein called llvSp, which was used as 'bait'. We ...


Evaluation Of The Basis And Effectiveness Of Habitat Assessments In Wetland Functional Assessment Methods, Amy Elizabeth Gardner May 2006

Evaluation Of The Basis And Effectiveness Of Habitat Assessments In Wetland Functional Assessment Methods, Amy Elizabeth Gardner

Biology Master’s Theses

I studied the basis and effectiveness of wetland assessment methods in providing habitat assessments. While it is well understood that wetlands and riparian areas provide important ecological functions and habitat for a wide variety of wildlife species, much is still to be learned about providing meaningful, accurate and repeatable methods for assessing them. I examined and evaluated four assessment methods to determine their accuracy and usefulness in assessing a site's provision of habitat.

One hypothesis I tested is that if the assessment methods studied provide an accurate assessment of wetland functions, then the resulting site scores for the methods ...