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Articles 1321 - 1338 of 1338

Full-Text Articles in Life Sciences

The Effects Of Tarp Wrapping On Blow Fly Oviposition During Decomposition Of Pigs, Natasha Lobosky, Jordan Stein Apr 2013

The Effects Of Tarp Wrapping On Blow Fly Oviposition During Decomposition Of Pigs, Natasha Lobosky, Jordan Stein

Symposium on Undergraduate Research and Creative Expression (SOURCE)

The effects of blow fly oviposition on 12 young, recently deceased pigs were studied during a one month period in the fall semester of 2012. This project follows similar techniques that Dr. Bugajski used during her research project at Purdue. Using tarp-wrapped bodies as the sole variable, bare pigs were held as the control. The data that were collected included the presence or absence of larvae or adult flies, daily temperatures, samples of both larvae and adult flies, and the start and end of maggot migration. The samples of larvae and adult flies were later identified in the lab and ...


Method For Instant Saccharomyces Cerevisiae Kill Of Samples, Melissa Kohner, Sara Dick Apr 2013

Method For Instant Saccharomyces Cerevisiae Kill Of Samples, Melissa Kohner, Sara Dick

Symposium on Undergraduate Research and Creative Expression (SOURCE)

It is essential when studying the circadian rhythm in cells to be able to effectively stop them in time. In this experiment, we tested what would be the most successful killing agent on Saccharomyces cerevisiae. Six different agents were tested at different concentrations and amounts. After the S. cerevisiae was added to the test tube containing the agent, it was streaked on a plate after 5 and 10 minutes. The plates were incubated and then checked for growth. Ethanol was the most efficient killing agent. After an effective killing agent is determined, it can be used in further experiments measuring ...


Diversity And Abundance Of Aquatic Macroinvertebrates Associated With Different Benthic Substrates In The East Branch Of The Little Calumet River, Halina Hopkins, Ali Olson Apr 2013

Diversity And Abundance Of Aquatic Macroinvertebrates Associated With Different Benthic Substrates In The East Branch Of The Little Calumet River, Halina Hopkins, Ali Olson

Symposium on Undergraduate Research and Creative Expression (SOURCE)

As part of an ongoing study of the effect of removing logjams to open the East Branch of the Little Calumet River for recreational purposes, we worked with park staff at the Indiana Dunes National Lakeshore on a study of the abundance and diversity of aquatic macroinvertebrates. We used a quantitative sampling technique to obtain samples of macroinvertebrates from five substrates in reaches of the river above and below four focal logjams. Diversity as measured by the Shannon-Weiner index varied with river substrate (sand=1.467, root wad=1.854, wood=1.64). Sand had the lowest average number of ...


Circadian Rhythm In Yeast, Jake Yablonowski, Michael Borchert Aug 2012

Circadian Rhythm In Yeast, Jake Yablonowski, Michael Borchert

Symposium on Undergraduate Research and Creative Expression (SOURCE)

Circadian rhythms are found in a variety of organisms and are involved in controlling activity of the organism. However, a circadian rhythm for yeast has not been discovered yet. The goal of this study is to find evidence for a circadian rhythm in yeast by looking for changes in GAPDH concentrations and peroxiredoxin levels. GAPDH and peroxiredoxin have been shown to be controlled by the circadian clock in many organisms. A continuous yeast culture is sampled every two hours over a period of about 104 hours. Each sample is processed and tested for the concentration of GAPDH using GAPDH reagent ...


Modeling Of Early Siv/Hiv Infection, Krista Schaefer, Cecilia Noecker, Kelly Zaccheo, Vitaly Gunasov, Judy Day, Yiding Yang Apr 2012

Modeling Of Early Siv/Hiv Infection, Krista Schaefer, Cecilia Noecker, Kelly Zaccheo, Vitaly Gunasov, Judy Day, Yiding Yang

Symposium on Undergraduate Research and Creative Expression (SOURCE)

Although HIV has infected over 20 million people worldwide, it is a rather poorly transmitted virus since less than 1 out of 100 to 1,000 acts of sexual intercourse results in virus transmission. The factors that could potentially explain why the probability of transmission is so small are poorly understood. It is nearly impossible to study HIV replication in the first 2-3 weeks of infection because the virus is undetectable until after that duration. By using stochastic simulations of mathematical models of early virus replication, we investigate how the duration of the eclipse phase prior to virus production (eclipse ...


Impacts Of The Species Elaeagnus Umbellate On The Soil And Water Quality Of The Pierce Cedar Creek Institute Ecosystem, Yacoub Aljobeh, Kristin Engerer Apr 2011

Impacts Of The Species Elaeagnus Umbellate On The Soil And Water Quality Of The Pierce Cedar Creek Institute Ecosystem, Yacoub Aljobeh, Kristin Engerer

Symposium on Undergraduate Research and Creative Expression (SOURCE)

The species Elaeagnus umbellate, more commonly known as autumn olive, is a shrub that is invasive to the United States and indigenous to East Asia. Even though the autumn olive is not native to North America, it was able to thrive and adapt to the new environment by using its ability to fix nitrogen. Nitrogen-fixing is a process where plants intake molecular nitrogen from the atmosphere and convert it into other forms of nitrogen that can be used by the plants. One of the nitrogen-fixing by-products is nitrate. Excessive amounts of nitrate can easily leach from the plant’s root ...


Siltation Related To Beaver Dam Decomposition In The Little Kankakee River, Mckenzie Kelly, Chris Bitcheno, Alyssa Thacker, Jon Gardow, Arissa Wallis Apr 2011

Siltation Related To Beaver Dam Decomposition In The Little Kankakee River, Mckenzie Kelly, Chris Bitcheno, Alyssa Thacker, Jon Gardow, Arissa Wallis

Symposium on Undergraduate Research and Creative Expression (SOURCE)

The Little Kankakee River (LKR) in LaPorte County, Indiana is an uncommon example of a good, cold-water fishery in northwest Indiana. However, the river has variable sedimentation levels; deep silt often covers gravel and sand, smothering invertebrates, a key food source for higher trophic organisms. The LKR contains a naturally-decomposing, abandoned beaver dam. The purpose of this study is to monitor its impact upon upstream and downstream silt levels. This research intends to identify possible sources of variability in silt levels, benefiting restoration teams in determining effects of dam removals. Furthermore, understanding the impact and pattern of silt levels could ...


Testing The Potential Of Using Fungi To Convert Human Waste Into Protein, Alex Zapata, Elizabeth Phillippi, Blair Mitchell, Jon Schoer, Michael Watters Apr 2011

Testing The Potential Of Using Fungi To Convert Human Waste Into Protein, Alex Zapata, Elizabeth Phillippi, Blair Mitchell, Jon Schoer, Michael Watters

Symposium on Undergraduate Research and Creative Expression (SOURCE)

We report on the results of a pilot experiment designed to test the potential of filamentous fungi (mold) to reduce solid waste (feces) while converting it into a consumable, high protein food product. Feces represent an untapped resource. Filamentous fungi are natural decomposers with the ability to use this resource. Many filamentous fungi are safe to eat. We examined growth in order to determine the conditions which maximized the rate of conversion of solid waste into fungal biomass. For this pilot, we compared the effect of different lengths of incubation, different methods of aeration, and different available surface area. The ...


Testing The Potential Of Using Fungi To Convert Human Waste Into Protein, Alex Zapata, Elizabeth Phillippi, Blair Mitchell, Jon Schoer, Michael K. Watters Jan 2011

Testing The Potential Of Using Fungi To Convert Human Waste Into Protein, Alex Zapata, Elizabeth Phillippi, Blair Mitchell, Jon Schoer, Michael K. Watters

Biology Faculty Presentations

We report on the results of a pilot experiment designed to test the potential of filamentous fungi (mold) to reduce solid waste (feces) while converting it into a consumable, high protein food product. Feces represent an untapped resource. Filamentous fungi are natural decomposers with the ability to use this resource. Many filamentous fungi are safe to eat. We examined growth in order to determine the conditions which maximized the rate of conversion of solid waste into fungal biomass. For this pilot, we compared the effect of different lengths of incubation, different methods of aeration, and different available surface area. The ...


National Park Service Nonnative Plant Control In The Indiana Dunes National Lakeshore, Jacob Halpin, Laurie Eberhardt, Laura Thompson Jan 2011

National Park Service Nonnative Plant Control In The Indiana Dunes National Lakeshore, Jacob Halpin, Laurie Eberhardt, Laura Thompson

Symposium on Undergraduate Research and Creative Expression (SOURCE)

Invasive plants have become a growing threat to plant diversity and hydrology in the Indiana Dunes National Lakeshore. Invasive plants compete with native plants for nutrients and sunlight, and certain invasive species have been known to completely take over certain areas of wetlands, nearly destroying entire ecosystems. The Dunes Lakeshore contains over 1,400 plants species and is one of the top ten most diverse national parks in the United States. The mission statement of the National Park Service is topreserve for the educational, inspirational, and recreational use of the public certain portions of the Indiana Dunes.” In order ...


Deer Browse Monitoring Of The Lupine Population At John Merle Coulter Prairie And The Trillium Population At Hildebrand Lake, Jana C. Cram, Heather Dulaney Jan 2011

Deer Browse Monitoring Of The Lupine Population At John Merle Coulter Prairie And The Trillium Population At Hildebrand Lake, Jana C. Cram, Heather Dulaney

Symposium on Undergraduate Research and Creative Expression (SOURCE)

A facet of ecological restoration practices in northwest Indiana involves monitoring and supporting the growth of various plants native to the region, including wild lupine (Lupinus perennis) and large-flowering trillium (Trillium grandiflorum). White-tailed deer are a common threat to these species because they eat, or browse, the flowers from these plants. This project investigated the amount of wild lupine and large-flowering trillium browsed by white-tailed deer at Coulter Prairie and Hildebrand Lake, respectively, over a four-year period. Plant counts are taken from permanent transect lines of 30 and 50 meters in length and measured in one meter quadrats along both ...


Improving Ecological Monitoring Of Restoration Sites In Northwest Indiana Through A Glisten-Nirmi Partnership., Danyi Harper, Don Meola Jan 2011

Improving Ecological Monitoring Of Restoration Sites In Northwest Indiana Through A Glisten-Nirmi Partnership., Danyi Harper, Don Meola

Symposium on Undergraduate Research and Creative Expression (SOURCE)

Northwest Indiana is an area of active ecological restoration with 169 documented restoration projects. However, the ability to assess the success of restoration projects is incomplete because monitoring of the progress of these restorations does not exist. NIRMI was created in 2010 to establish a system of evaluating and quantifying plant species in local habitat restoration projects throughout Northwest Indiana. NIRMI uses a standardized approach to collect data that is placed within an open-access database for use by other researchers, restoration groups, and individuals in evaluation of restoration efforts. Data collection uses the CVS-EEP protocol for recording vegetation, a standard ...


Cyp704b1 Is A Long-Chain Fatty Acid V-Hydroxylase Essential For Sporopollenin Synthesis In Pollen Of Arabidopsis, Anna A. Dobritsa, Jay Shrestha, Marc Morant, Franck Pinot, Michiyo Matsuno, Rob Swanson, Birger Lindberg Møller, Daphne Preuss Jan 2009

Cyp704b1 Is A Long-Chain Fatty Acid V-Hydroxylase Essential For Sporopollenin Synthesis In Pollen Of Arabidopsis, Anna A. Dobritsa, Jay Shrestha, Marc Morant, Franck Pinot, Michiyo Matsuno, Rob Swanson, Birger Lindberg Møller, Daphne Preuss

Biology Faculty Publications

Sporopollenin is the major component of the outer pollen wall (exine). Fatty acid derivatives and phenolics are thought to be itsmonomeric building blocks, but the precise structure, biosynthetic route, and genetics of sporopollenin are poorly understood.Based on a phenotypic mutant screen in Arabidopsis (Arabidopsis thaliana), we identified a cytochrome P450, designatedCYP704B1, as being essential for exine development. CYP704B1 is expressed in the developing anthers. Mutations in CYP704B1result in impaired pollen walls that lack a normal exine layer and exhibit a characteristic striped surface, termed zebraphenotype. Heterologous expression of CYP704B1 in yeast cells demonstrated that it catalyzes v-hydroxylation of long-chainfatty ...


Incidence And Post-Pollination Mechanisms Of Nonrandom Mating In Arabidopsis Thaliana, Ann L. Carlson, Megan Telligman, Rob Swanson Jan 2009

Incidence And Post-Pollination Mechanisms Of Nonrandom Mating In Arabidopsis Thaliana, Ann L. Carlson, Megan Telligman, Rob Swanson

Biology Faculty Publications

Compatible pollinations from many differenttaxa display nonrandom mating. Here we describe a systemfor examining questions of nonrandom mating in Arabidopsisthaliana. Using this system, we demonstrate thatArabidopsis thaliana displays nonrandom mating betweendistinct accessions. Statistical analysis of these data demonstratesaspects of both pollen competition and male–female complementarity in these matings. Cytologicalexperiments implicate pollen germination and pollen tubegrowth rates as possible causal factors in these nonrandommating efficiencies.


Lap3, A Novel Plant Protein Required For Pollen Development, Is Essential For Proper Exine Formation, Anna A. Dobritsa, Shuh-Ichi Nishikawa, Daphne Preuss, Ewa Urbanczyk-Wochniak, Lloyd W. Sumner, Adam Hammond, Ann L. Carlson, Rob Swanson Jan 2009

Lap3, A Novel Plant Protein Required For Pollen Development, Is Essential For Proper Exine Formation, Anna A. Dobritsa, Shuh-Ichi Nishikawa, Daphne Preuss, Ewa Urbanczyk-Wochniak, Lloyd W. Sumner, Adam Hammond, Ann L. Carlson, Rob Swanson

Biology Faculty Publications

We isolated lap3-1 and lap3-2 mutants in ascreen for pollen that displays abnormal stigma binding.Unlike wild-type pollen, lap3-1 and lap3-2 pollen exine isthinner, weaker, and is missing some connections betweentheir roof-like tectum structures. We describe the mappingand identification of LAP3 as a novel gene that contains arepetitive motif found in b-propeller enzymes. Insertionmutations in LAP3 lead to male sterility. To investigatepossible roles for LAP3 in pollen development, we assayedthe metabolite profile of anther tissues containing developingpollen grains and found that the lap3-2 defect leadsto a broad range of metabolic changes. The largest changeswere seen in levels of a straight-chain ...


Strain-Dependent Relationship Between Growth Rate And Hyphal Branching In Neurospora Crassa, Michael K. Watters, Erik Lindamood, Margaret Meunich, Ryan Vetor Jan 2008

Strain-Dependent Relationship Between Growth Rate And Hyphal Branching In Neurospora Crassa, Michael K. Watters, Erik Lindamood, Margaret Meunich, Ryan Vetor

Biology Faculty Publications

In a previous study of branch frequency in Neurospora crassa focused on the wild-type, no relationship between growth rate and the frequency of hyphal branching was observed. In subsequent experiments, it became clear that while this independence is valid for the wild type and most mutant strains, it fails to hold for a subset of morphological mutants. This study distinguishes a subset of Neurospora morphological mutants for their morphological response to altered growth rate. Growth rates are altered using two different methods: reduced temperature and nutrient-deficient media. This should assure that the observed effect is not due to simple conditional ...


Callose (Β-1,3 Glucan) Is Essential For Arabidopsis Pollen Wall Patterning, But Not Tube Growth, Rob Swanson Oct 2005

Callose (Β-1,3 Glucan) Is Essential For Arabidopsis Pollen Wall Patterning, But Not Tube Growth, Rob Swanson

Biology Faculty Publications

Background: Callose (β-1,3 glucan) separates developing pollen grains, preventing their underlying walls (exine) from fusing. The pollen tubes that transport sperm to female gametes also contain callose, both in their walls as well as in the plugs that segment growing tubes. Mutations in CalS5, one of several Arabidopsis β-1,3 glucan synthases, were previously shown to disrupt callose formation around developing microspores, causing aberrations in exine patterning, degeneration of developing microspores, and pollen sterility. Results: Here, we describe three additional cals5 alleles that similarly alter exine patterns, but instead produce fertile pollen. Moreover, one of these alleles (cals5-3) resulted ...


A Conserved Ubiquitin Ligase Of The Nuclear Envelope/Endoplasmic Reticulum That Functions In Both Er-Associated And Mat A2 Repressor Degradation, Rob Swanson Aug 2001

A Conserved Ubiquitin Ligase Of The Nuclear Envelope/Endoplasmic Reticulum That Functions In Both Er-Associated And Mat A2 Repressor Degradation, Rob Swanson

Biology Faculty Publications

Substrate discrimination in the ubiquitin–proteasome system is believed to be dictated by specific combinations of ubiquitin–protein ligases (E3s) and ubiquitin-conjugating enzymes (E2s). Here we identify Doa10/Ssm4 as a yeast E3 that is embedded in the endoplasmic reticulum (ER)/nuclear envelope yet can target the soluble transcription factor Mat2. Doa10 contains an unusual RING finger, which has ubiquitin-ligase activity in vitro and is essential in vivo for degradation of 2 via its Deg1 degradation signal. Doa10 functions with two E2s, Ubc6 and Ubc7, to ubiquitinate Deg1-bearing substrates, and it is also required for the degradation of at least ...