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Life Sciences Commons

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Valparaiso University

2013

Articles 1 - 4 of 4

Full-Text Articles in Life Sciences

Circadian Rhythms In Saccharomyces Cerevisiae, Khyla Rose Alorro, Sean Mcnabney Jul 2013

Circadian Rhythms In Saccharomyces Cerevisiae, Khyla Rose Alorro, Sean Mcnabney

Symposium on Undergraduate Research and Creative Expression (SOURCE)

Circadian rhythms are endogenous, time-oriented cycles that cause physical or behavioral changes in organisms. While several studies suggest that such rhythms are ubiquitous for life, recent experiments demonstrate that the regulatory mechanisms behind them differ for each organism. Little is known about the molecular machinery that governs the circadian clock in Saccharomyces cerevisiae, but its output appears to directly influence the enzymes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and peroxiredoxin. This project centered on linking GAPDH concentrations to various stages of the circadian rhythm in order to inductively determine components of the circadian clock. Spectroscopic assays and Western blots were used to determine ...


The Effects Of Tarp Wrapping On Blow Fly Oviposition During Decomposition Of Pigs, Natasha Lobosky, Jordan Stein Apr 2013

The Effects Of Tarp Wrapping On Blow Fly Oviposition During Decomposition Of Pigs, Natasha Lobosky, Jordan Stein

Symposium on Undergraduate Research and Creative Expression (SOURCE)

The effects of blow fly oviposition on 12 young, recently deceased pigs were studied during a one month period in the fall semester of 2012. This project follows similar techniques that Dr. Bugajski used during her research project at Purdue. Using tarp-wrapped bodies as the sole variable, bare pigs were held as the control. The data that were collected included the presence or absence of larvae or adult flies, daily temperatures, samples of both larvae and adult flies, and the start and end of maggot migration. The samples of larvae and adult flies were later identified in the lab and ...


Method For Instant Saccharomyces Cerevisiae Kill Of Samples, Melissa Kohner, Sara Dick Apr 2013

Method For Instant Saccharomyces Cerevisiae Kill Of Samples, Melissa Kohner, Sara Dick

Symposium on Undergraduate Research and Creative Expression (SOURCE)

It is essential when studying the circadian rhythm in cells to be able to effectively stop them in time. In this experiment, we tested what would be the most successful killing agent on Saccharomyces cerevisiae. Six different agents were tested at different concentrations and amounts. After the S. cerevisiae was added to the test tube containing the agent, it was streaked on a plate after 5 and 10 minutes. The plates were incubated and then checked for growth. Ethanol was the most efficient killing agent. After an effective killing agent is determined, it can be used in further experiments measuring ...


Diversity And Abundance Of Aquatic Macroinvertebrates Associated With Different Benthic Substrates In The East Branch Of The Little Calumet River, Halina Hopkins, Ali Olson Apr 2013

Diversity And Abundance Of Aquatic Macroinvertebrates Associated With Different Benthic Substrates In The East Branch Of The Little Calumet River, Halina Hopkins, Ali Olson

Symposium on Undergraduate Research and Creative Expression (SOURCE)

As part of an ongoing study of the effect of removing logjams to open the East Branch of the Little Calumet River for recreational purposes, we worked with park staff at the Indiana Dunes National Lakeshore on a study of the abundance and diversity of aquatic macroinvertebrates. We used a quantitative sampling technique to obtain samples of macroinvertebrates from five substrates in reaches of the river above and below four focal logjams. Diversity as measured by the Shannon-Weiner index varied with river substrate (sand=1.467, root wad=1.854, wood=1.64). Sand had the lowest average number of ...