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Life Sciences Commons

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Valparaiso University

2001

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Full-Text Articles in Life Sciences

A Conserved Ubiquitin Ligase Of The Nuclear Envelope/Endoplasmic Reticulum That Functions In Both Er-Associated And Mat A2 Repressor Degradation, Rob Swanson Aug 2001

A Conserved Ubiquitin Ligase Of The Nuclear Envelope/Endoplasmic Reticulum That Functions In Both Er-Associated And Mat A2 Repressor Degradation, Rob Swanson

Biology Faculty Publications

Substrate discrimination in the ubiquitin–proteasome system is believed to be dictated by specific combinations of ubiquitin–protein ligases (E3s) and ubiquitin-conjugating enzymes (E2s). Here we identify Doa10/Ssm4 as a yeast E3 that is embedded in the endoplasmic reticulum (ER)/nuclear envelope yet can target the soluble transcription factor Mat2. Doa10 contains an unusual RING finger, which has ubiquitin-ligase activity in vitro and is essential in vivo for degradation of 2 via its Deg1 degradation signal. Doa10 functions with two E2s, Ubc6 and Ubc7, to ubiquitinate Deg1-bearing substrates, and it is also required for the degradation of at least ...