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University of Tennessee, Knoxville

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Articles 1 - 30 of 4139

Full-Text Articles in Life Sciences

Developing A Hands-On Food Science Curriculum With Bilingual, Elementary-Aged, Hispanic Heritage Students, Jaime Leia Ragos May 2019

Developing A Hands-On Food Science Curriculum With Bilingual, Elementary-Aged, Hispanic Heritage Students, Jaime Leia Ragos

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

In the United States alone there are over 48 million cases of foodborne illness, with most of these cases deriving from food cooked in the home (Young 2016). Many themes have been assessed such as confidence, knowledge, habits, taste in food preferences, and societal/social influences (Young 2016). However, when food safety curricula are targeted toward younger populations, including young adults and children, attitudes and behavior change (Mullan 2018 and Young 2016). As there is a lack in home economics education in today’s current curriculum, students and their families may not know food safety behaviors (Finch 2005; Young 2016 ...


Effect Of Turmeric On The Promoter Activity Of The Cyp6a8 Gene Of Drosophila Melanogaster, Alexa Stroh May 2019

Effect Of Turmeric On The Promoter Activity Of The Cyp6a8 Gene Of Drosophila Melanogaster, Alexa Stroh

Chancellor’s Honors Program Projects

No abstract provided.


Observing The Effects Of Aspergillus Oryzae On Combating The Consequences Of Heat Stress In Lactating Dairy Cows, Abigail Lorraine Kesterson May 2019

Observing The Effects Of Aspergillus Oryzae On Combating The Consequences Of Heat Stress In Lactating Dairy Cows, Abigail Lorraine Kesterson

Chancellor’s Honors Program Projects

No abstract provided.


A Profile Of Physical Activity In Knoxville Parks, Margaret R. Bailey May 2019

A Profile Of Physical Activity In Knoxville Parks, Margaret R. Bailey

Chancellor’s Honors Program Projects

No abstract provided.


The Role Of T-Cell Lymphoma Invasion And Metastasis 2 (Tiam2) In The Barrier Function Of Mammary Epithelial Cells Following Streptococcus Uberis Inoculation, Catherine E. Donnell May 2019

The Role Of T-Cell Lymphoma Invasion And Metastasis 2 (Tiam2) In The Barrier Function Of Mammary Epithelial Cells Following Streptococcus Uberis Inoculation, Catherine E. Donnell

Chancellor’s Honors Program Projects

No abstract provided.


The Impact Of Race And Affect On Infant Visual Attention To Faces, Emily Kathryn Grimes May 2019

The Impact Of Race And Affect On Infant Visual Attention To Faces, Emily Kathryn Grimes

Chancellor’s Honors Program Projects

No abstract provided.


Developing A Hands-On Food Science Curriculum With Bilingual Elementary-Aged Hispanic Heritage Students, Jaime Leia Ragos May 2019

Developing A Hands-On Food Science Curriculum With Bilingual Elementary-Aged Hispanic Heritage Students, Jaime Leia Ragos

Chancellor’s Honors Program Projects

No abstract provided.


Food Acquisition Strategies Within Theresienstadt Ghetto: An Analysis Of Oral Testimonies, Jaime L. Marquis May 2019

Food Acquisition Strategies Within Theresienstadt Ghetto: An Analysis Of Oral Testimonies, Jaime L. Marquis

Chancellor’s Honors Program Projects

No abstract provided.


Acoustic Monitoring Of American Woodcock, Evan J. Buck May 2019

Acoustic Monitoring Of American Woodcock, Evan J. Buck

Chancellor’s Honors Program Projects

No abstract provided.


Applications Of Optimization Modeling In Multi-Disciplinary Engineering Research, Anton F. Astner, Ekramul Haque Ehite, Yang Li, Colin Sasthav Apr 2019

Applications Of Optimization Modeling In Multi-Disciplinary Engineering Research, Anton F. Astner, Ekramul Haque Ehite, Yang Li, Colin Sasthav

Biosystems Engineering and Soil Science Publications and Other Works

Optimization modeling is the process of selection of the best solution to a design problem using predetermined constraints from a set of prospective solutions. Increased computing power has made optimization solvers readily available for business/research needs. For example, Microsoft Excel has a simple, but robust solver. Such solvers can model linear, nonlinear, and integer programming problems that are limited in size. This study shows the use of the optimization model solvers in various research contexts.


Exploring The Structure-Activity Relationship Of Various Seh Inhibitors In Promoting Brown Adipogenesis, Sue Lim Choi Apr 2019

Exploring The Structure-Activity Relationship Of Various Seh Inhibitors In Promoting Brown Adipogenesis, Sue Lim Choi

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Background: Brown adipose tissue has emerged as a novel target for obesity prevention and treatment due to its responsibility for heat production. Soluble epoxide hydrolase (sEH) is a cytosolic enzyme that degrades epoxy fatty acids (EpFAs) (lipid signaling molecules) into inactive diols. Potent sEH inhibitors (sEHIs) are beneficial for many chronic diseases as they stabilize endogenous EpFAs by blocking the degradation. Our preliminary results have shown that trans-4- [4-(3-adamantan-1-yl-ureido)-cyclohexyloxy]-benzoic acid (t-AUCB), a potent sEH inhibitor, dose-dependently promotes brown adipogenesis.

Objectives: The objective of this study is to investigate the structure and activity relationship of various sEHIs with ...


The Function Of Ecdysone And Inhibiting Programmed Cell Death In Death Class Iii Neurons Of Drosophila Melanogaster, Nikolas Richard Likourentzos Apr 2019

The Function Of Ecdysone And Inhibiting Programmed Cell Death In Death Class Iii Neurons Of Drosophila Melanogaster, Nikolas Richard Likourentzos

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

The inhibition of programmed cell death is a factor believed to be responsible for the survival of cancer cells. Using Drosophila as models, factors contributing to the process of programmed cell death can be studied. Neurons die throughout Drosophila melanogaster development to allow the addition of new neurons. The groups of neurons programmed to die are Death Class I neurons, Death Class II neurons, and Death Class III neurons. Ecdysone is a hormone responsible for the timing of programmed cell death (PCD) in the Death Class neurons. Elevated levels of ecdysone are associated with the initiation of PCD of Death ...


The Full Genome Sequence Of An Antarctic Microbe Constructed Using A Rapid, Portable Sequencer And A Hybrid Assembly, Bruce Wyatt Boles Apr 2019

The Full Genome Sequence Of An Antarctic Microbe Constructed Using A Rapid, Portable Sequencer And A Hybrid Assembly, Bruce Wyatt Boles

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Microbial genomes contain combinations of nucleotides that code for genes which subsequently determines the function of the cell. This information provides significant insight into the unique niche of microbes from extreme environments. Here we use two sequencing technologies, the Oxford Nanopore MinION with accompanying software and Illumina HiSeq, to generate a hybrid genome for a Shewanella strain isolated from an Antarctic glacier. Bioinformatic programs, Albacore and SPAdes, allowed us to decrease the time of genome assembly while also obtaining a large quantity of information related to the Shewanella sp. Our combined approach yielded a high quality genome assembly 5.3 ...


What’S For Dinner? Different Carbon Compounds Influence Host Metabolism In A Model Roseobacter-Roseophage System, Kaylee Rae Jacobs Apr 2019

What’S For Dinner? Different Carbon Compounds Influence Host Metabolism In A Model Roseobacter-Roseophage System, Kaylee Rae Jacobs

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Viruses that infect bacteria (temperate phages) engage in complex interactions with their hosts. These phages can have two life cycles: lytic and lysogenic. For the latter, the phage can integrate its genome into that of its host and harmlessly replicate alongside with it. Conversely, upon induction, these integrated viruses can excise from their host genome and initiate the lytic cycle. A current paradigm is that phage induction is in response to host cell stress. However, in the absence of stressors, a low level of induction in a population occurs. This poorly understood phenomenon is referred to as spontaneous prophage induction ...


A Cell Cycle Cue Triggers Cell Growth Resumption After Division, Afton R. Russell Apr 2019

A Cell Cycle Cue Triggers Cell Growth Resumption After Division, Afton R. Russell

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

The decoupling of the fundamental processes of cell division and growth is important for maintaining cell integrity. Through a chemical approach, we delayed the clock that controls when cells separate, uncoupling cell division and cell growth. This led to polarized cell growth before the cells completed separation. Using fluorescent markers to denote the cell’s stage in the cell cycle, we observed that only cells that were in mitosis exhibited this uncoupling. Previously it was thought that growth resumption occurred after completion of cell division, but this observation suggests that growth is triggered earlier, from a mitotic cue. This mitotic ...


Cell Separation Delay And Membrane Trafficking Defects In Cdc42 Gap Mutants, Haylee Grace Young Apr 2019

Cell Separation Delay And Membrane Trafficking Defects In Cdc42 Gap Mutants, Haylee Grace Young

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Cytokinesis is the final step in cell division, where a cell separates into two daughter cells. Cytokinesis involves many steps that must be organized in a spatiotemporal manner. In many eukaryotes, it involves the assembly and constriction of an actomyosin ring. The fission yeast Schizosaccharomyces pombe serves as a good model system to study cytokinesis because they divide via actomyosin-dependent-cytokinesis.

The Rho-family of small GTPases are molecules involved in the regulation of cell growth and division. The GTPase Cdc42 helps promote timely onset of ring constriction and septum formation in fission yeast. Studies with many other organisms show that Cdc42 ...


Circadian Rhythmicity And Neurodevelopment Of Disco And Grim Mutations In Drosophila Melanogaster, John Patrick Story Apr 2019

Circadian Rhythmicity And Neurodevelopment Of Disco And Grim Mutations In Drosophila Melanogaster, John Patrick Story

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

The death gene grim and its pathway for apoptosis has been studied extensively in Drosophila Melanogaster. The effects of grim mutations on circadian neurodevelopment and locomotor assays have yet to be investigated. Mutations in the gene disconnected (disco) has been shown to disrupt the normal development of the circadian circuitry, specifically the small ventro-lateral neurons (s-LNv’s). Which has shown to severely decrease rhythmicity during free-running periods. Alternatively, we have observed an increase in rhythmicity during free-running periods in grim mutations. Our goal is to investigate the neurodevelopment of the circadian circuitry and their associated locomotor activities in these Drosophila ...


Microbe Hunters: Searching For Anammox Bacteria In The Tennessee Aquarium, Claire Elbon Apr 2019

Microbe Hunters: Searching For Anammox Bacteria In The Tennessee Aquarium, Claire Elbon

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Ammonium and nitrite are toxic metabolic waste products generated by aquatic macroorganisms. They are of particular concern in closed systems, such as commercial aquaria. Typically, biological filtration systems are employed to regulate levels of toxic N species as they are more cost-efficient compared to water removal and replacement. Microbial communities that reside in these systems play vital roles in transformation of toxic N species. Commonly, nitrite and ammonium are converted into nitrate via nitrification. However, even nitrate is toxic at higher concentrations. Bacteria belonging to the phylum Planctomycetes can transform ammonium and nitrite to N2 via anaerobic ammonium oxidation (anammox ...


Ultramicrobacteria Genome Database Project, Abdullah A. Salim, Tien Tran, Andrew Putt, Terry C. Hazen Apr 2019

Ultramicrobacteria Genome Database Project, Abdullah A. Salim, Tien Tran, Andrew Putt, Terry C. Hazen

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Ultramicrobacteria (UMB) are a largely uncultured, globally abundant, and metabolically active group of bacteria. UMB have cell diameters ≤0.3μm, cell volumes ≤0.1 μm3, and small streamlined genomes. Recent findings indicate that UMB aid in bioremediation and nutrient cycling, but future investigations and comprehension of current findings are skewed by highly variable nomenclature and a lack of databases for functional, genomic, geochemical, or spatial data specific to candidate UMB. We aim to develop a user-friendly open-access database of various UMB candidates linked to an open-access online map where researchers can gather genomic, spatial, and geochemical data. Our comprehensive review ...


Acute Social Defeat-Induced Neuroinflammation In The Vmpfc Of Syrian Hamsters Via Microglial Activation, Thomas Clarity Apr 2019

Acute Social Defeat-Induced Neuroinflammation In The Vmpfc Of Syrian Hamsters Via Microglial Activation, Thomas Clarity

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Research suggests causal relationships between neuroinflammation and stress-related psychopathologies. Exposure to moderate or chronic psychological stress in rodents leads to increased activation of microglia, the brain’s resident immune cells. The ventral medial prefrontal cortex (vmPFC) is a key limbic region involved in top-down regulation of psychological stress and mediates the deleterious effects of microglial activity following prolonged restraint stress. While there is a growing body of literature indicating that chronic social defeat increases microglial activity in the vmPFC, there has been little research investigating the effects of acute social defeat stress. Here, we used an acute social defeat paradigm ...


Development Of A Molecular Genetic Method For Characterizing Amphibian Diets, Alexander T. Funk Apr 2019

Development Of A Molecular Genetic Method For Characterizing Amphibian Diets, Alexander T. Funk

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

DEVELOPMENT OF A MOLECULAR GENETIC METHOD FOR CHARACTERIZING AMPHIBIAN DIETS.Alexander Funk* and Todd W. Pierson, Department of Ecology and Evolutionary Biology, University of Tennessee Knoxville, afunk4@vols.utk.edu

Dietary studies can provide key insights into the ecology and behavior of animals. Methods common in dietary studies of amphibians (e.g., gastric lavage, fecal analysis, dissection) often fail to identify prey beyond the level of Order, are time intensive, and can be biased against soft-bodied prey. Here, we are developing a DNA metabarcoding assay to characterize amphibian diets from non-invasive fecal samples. In DNA metabarcoding, barcoding loci from mixed ...


The Neural Circuitry Of Sweaty Palms: A Neuroimaging Meta-Analysis, Sydnie Colette Toler, Derek C. Wenger Jan 2019

The Neural Circuitry Of Sweaty Palms: A Neuroimaging Meta-Analysis, Sydnie Colette Toler, Derek C. Wenger

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Emotion and cognitive processes often cause autonomic nervous system responses. A common example is when the palms of your hands become sweaty before giving a speech or when a police officer is driving behind you. Increased sweat on the palms is a marker of sympathetic nervous system (SNS) activity. The aim of this study was to identify networks of brain activity that make our palms sweat. We compiled a list of neuroimaging studies that report brain activity associated with palm sweat. Our search resulted in sixteen studies, comprised of 251 participants. We used activation likelihood estimation analysis to determine nonrandom ...


Differentiation Of Neurons And Glia For Use In Cellular Connectomics, Jacob T. Brettin Dec 2018

Differentiation Of Neurons And Glia For Use In Cellular Connectomics, Jacob T. Brettin

Chancellor’s Honors Program Projects

No abstract provided.


Aspects Of The Reproductive Biology And Growth Of The Mississippi Silvery Minnow, Hybognathus Nuchalis (Agassiz, 1855) (Teleostei: Cyprinidae) From The Pearl River, Louisiana., Arely Ramírez-García, Kyle R. Piller Nov 2018

Aspects Of The Reproductive Biology And Growth Of The Mississippi Silvery Minnow, Hybognathus Nuchalis (Agassiz, 1855) (Teleostei: Cyprinidae) From The Pearl River, Louisiana., Arely Ramírez-García, Kyle R. Piller

Southeastern Fishes Council Proceedings

The reproductive biology and growth of the Mississippi Silvery Minnow, Hybognathus nuchalis, is described from multiple sites in the Pearl River, Louisiana. Individuals were collected from August 2011 to August 2012. Ovarian weights, expressed as a percentage of body weights, peaked in December. Size structure ranged from 29.0 to 60.0 mm SL for females and 25.0 to 56.0 mm SL for males. Mature ova were found from November to January. Females reached first maturity (L50) at 37.0 mm SL and L50 for males is at 41.0 mm SL. Sex ratio (females:males) is biased ...


Multi-Metric Conservation Assessment For The Imperiled Clinch Dace, Michael James Moore, Donald J. Orth, Eric M. Hallerman Nov 2018

Multi-Metric Conservation Assessment For The Imperiled Clinch Dace, Michael James Moore, Donald J. Orth, Eric M. Hallerman

Southeastern Fishes Council Proceedings

Planning frameworks allow managers to spatially prioritize actions to promote species conservation. Traditional aquatic conservation planning frameworks are often organized at the ecological community or ecosystem level, which often neglect imperiled taxa occupying species-poor assemblages. In this study, we develop a multi-metric conservation assessment for the 15 geographically distinct candidate conservation areas (CCAs) occupied by the imperiled Clinch Dace (Chrosomus sp. cf. saylori). Clinch Dace habitat is threatened by anthropogenic landscape alterations, especially for coal mining and timber harvest. Our framework used four metrics to assess the conservation value of each subpopulation of Clinch Dace namely: “habitat condition”, “viability”, conservation ...


Reproductive Timing Of The Scarlet Shiner (Lythrurus Fasciolaris) In Northern Alabama, Bruce Stallsmith, Toacca Taylor, Chelsie Smith Sep 2018

Reproductive Timing Of The Scarlet Shiner (Lythrurus Fasciolaris) In Northern Alabama, Bruce Stallsmith, Toacca Taylor, Chelsie Smith

Southeastern Fishes Council Proceedings

The Scarlet Shiner (Lythrurus fasciolaris) is a cyprinid species widely distributed in parts of the Ohio, Cumberland and Tennessee river drainages of the United States. The objective of this study was to determine the Scarlet Shiner’s reproductive schedule. Maturation of ovaries and oocytes was determined through the categorization of developmental stages from early maturing to ripe, along with the calculation of monthly gonadosomatic index (GSI) measurements over two reproductive seasons in 2012 and 2015. In both years reproductive competence began in April. Average monthly GSI for females peaked in May, followed by a slow decline through August. Average clutch ...


Updated Distributional Records Of Selected Kentucky Fishes, David J. Eisenhour, Matthew R. Thomas, J. Jacob Culp, Michael C. Compton, Stephanie L. Brandt, Rodney Pierce Aug 2018

Updated Distributional Records Of Selected Kentucky Fishes, David J. Eisenhour, Matthew R. Thomas, J. Jacob Culp, Michael C. Compton, Stephanie L. Brandt, Rodney Pierce

Southeastern Fishes Council Proceedings

Distribution records for 15 fish species of conservation interest are included for Kentucky. Notropis dorsalis, previously undocumented in Kentucky, is reported for the first time (Mayfield Creek drainage). Also reported are state rediscoveries of three species thought to be extirpated from Kentucky: Ichthyomyzon gagei (Tennessee River drainage), Hemitremia flammea (Lower Cumberland and Green river drainages), and Hybopsis amnis (upper Cumberland River drainage). Unreported drainage records comprise nine species: Ichthyomyzon castaneus (Salt River drainage and Upper Green River drainage); Ichthyomyzon unicuspis (Salt River drainage and upper Big Sandy River drainage); Campostoma pullum (Tennessee River drainage); Umbra limi (Obion Creek drainage); Lepomis ...


Investigating The Role Of The Rough Deal Protein In Spindle Assembly Complex Signaling, Ryan P. Mihealsick Aug 2018

Investigating The Role Of The Rough Deal Protein In Spindle Assembly Complex Signaling, Ryan P. Mihealsick

Chancellor’s Honors Program Projects

No abstract provided.


Improving The Analysis Of T Cell Movement, Viktor Zenkov May 2018

Improving The Analysis Of T Cell Movement, Viktor Zenkov

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Vaccine-induced T cells play an important role in combating malaria by eliminating infection in the liver stage. However, as millions of hepatocytes inhabit a mouse liver and only some are infected, how T cells locate the infection site and eliminate infection remains poorly understood. Are T cells moving intentionally toward parasites, or randomly successful? To answer this, I used timed position data of malaria-specific T cells, non-specific control T cells, and a parasite, obtained from experiments in a mouse liver; I performed analyses with the null hypothesis that T cells move randomly. I used two metrics, based on distances from ...


Acute Social Defeat Stress Induces Microglial Activation In Key Limbic Regions, Thomas Clarity May 2018

Acute Social Defeat Stress Induces Microglial Activation In Key Limbic Regions, Thomas Clarity

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Research suggests a causal relationship between neuroinflammation and stress-related psychopathologies. Exposure to moderate psychological stress in rodent models leads to elevated markers of immune activity in the brain, for example, microglia. Research has shown that tail shock stress can prime the subsequent, immune-challenged activation of microglia, which can lead to a degradative, proinflammatory response. Although social defeat is an ethologically relevant model of acute stress, there has been little research investigating the effects of acute social defeat stress on immune activity. Here, we used an acute social defeat paradigm in Syrian hamsters consisting of three, 5-minute aggressive encounters in the ...