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University of Nebraska - Lincoln

Dissertations, Theses, & Student Research in Food Science and Technology

Rapid method

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Evaluation Of Qualitative Food Allergen Detection Methods And Cleaning Validation Approaches, Rachel C. Courtney May 2016

Evaluation Of Qualitative Food Allergen Detection Methods And Cleaning Validation Approaches, Rachel C. Courtney

Dissertations, Theses, & Student Research in Food Science and Technology

Allergen control plans are increasingly used by the food industry to prevent allergen cross-contact and validation of these plans involves methods to detect allergen protein residues. A commonly used rapid allergen detection method is lateral flow devices, although research about their validation is lacking. The objective of this research was to investigate lateral flow devices, their specificity and sensitivity to milk proteins and milk-derived ingredients, swabbing conditions, and applications in cleaning validation.

Several lateral flow devices advertised to detect total milk did not detect whey proteins or whey-derived ingredients. The overload level of the kits was highly variable (ranging from ...


Identification And Characterization Of Salmonella Serotypes Isolated From Pork And Poultry From Commercial Sources, Yulie Edith Meneses-GonzáLez Nov 2010

Identification And Characterization Of Salmonella Serotypes Isolated From Pork And Poultry From Commercial Sources, Yulie Edith Meneses-GonzáLez

Dissertations, Theses, & Student Research in Food Science and Technology

Salmonella is one of the leading causes of foodborne illness worldwide, and it is estimated that 1.4 million infections occur annually in the U.S. alone. The Premi®Test Salmonella (PTS), is a potential tool for rapid detection and identification of Salmonella serovars. The objective of this project was to evaluate the use of the PTS system as a serotyping tool to identify pork and poultry isolates obtained from vertically integrated operations and to characterize their antibiotic resistance. Two hundred isolates were evaluated. All isolates were serotyped using the traditional Kaufmann-White scheme and the PTS system. Among the isolates ...