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University of Nebraska - Lincoln

Dissertations, Theses, & Student Research in Food Science and Technology

QPCR

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Development Of A Rapid Detection And Quantification Method For Yeasts And Molds In Dairy Products, Brandon Nguyen Jan 2017

Development Of A Rapid Detection And Quantification Method For Yeasts And Molds In Dairy Products, Brandon Nguyen

Dissertations, Theses, & Student Research in Food Science and Technology

A rapid quantitative PCR (qPCR) method was developed for the detection and quantification of fungi that are potentially present in dairy commodities. Genes of interest that were considered and used in the method development were the following: 18S rRNA, actin, beta-tubulin, and elongation factor 1-alpha. The following organisms were screened in this method development: Galactomyces candidus, Debaryomyces hansenii, Yarrowia lipolytica, Penicillium roqueforti, Penicillium verrocosum and Cladosporium cladosporioides. The developed method has a standard curve based on the organism, Galactomyces candidus, and the primers based on the elongation factor 1-alpha gene. Using this yeast and primers, this method can detect fungal ...


Evidence Of Transmission Of Escherichia Coli O157:H7 To The Tissues Or Phyllo-Plane Of Wheat, From Contaminated Soil, Seeds Or Water, Bismarck Antonio Martinez Nov 2012

Evidence Of Transmission Of Escherichia Coli O157:H7 To The Tissues Or Phyllo-Plane Of Wheat, From Contaminated Soil, Seeds Or Water, Bismarck Antonio Martinez

Dissertations, Theses, & Student Research in Food Science and Technology

Escherichia coli O157:H7 is a human pathogen that can cause a wide spectrum of disease symptoms, such as bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS). Escherichia coli O157:H7 illness are mainly associated with undercooked beef; however, in recent years outbreaks have been linked to fresh produce such as spinach, lettuce, and sprouts. In 2009, flour was implicated as a contamination source in the consumption of raw cookie dough resulting in 77 illness-cases. The objective of this research was to determine the possible route of transmission of E. coli O157:H7 into the phyllo-plane of wheat using ...