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University of Nebraska - Lincoln

Dissertations, Theses, & Student Research in Food Science and Technology

Prebiotics

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Adaptation And Validation Of Food Product Specific Analytical Methods For Monitoring Prebiotics Present In Different Types Of Processed Food Matrices, Rebbeca M. Duar Dec 2011

Adaptation And Validation Of Food Product Specific Analytical Methods For Monitoring Prebiotics Present In Different Types Of Processed Food Matrices, Rebbeca M. Duar

Dissertations, Theses, & Student Research in Food Science and Technology

Prebiotic carbohydrates are now added to a variety of processed foods to beneficially affect the gut microbial composition and activities. However, published data remain limited on the stability of prebiotics during food processes, such as baking, extrusion, pasteurization, high temperature heating, low pH condition, etc. As the complexity of the food matrix may also affect the ability to test for prebiotics, product specific analytical methods, including UV-vis spectroscopy, GC and HPLC, were developed and validated to monitor the stability of FOS, inulin and GOS in different types of processed food (breakfast cereal, cookie, muffin, sports drink and a nutritional bar ...


Studies On The Adherence Properties Of Plant Lectins And Bacterial Adhesins And Their Inhibition By Prebiotic Oligosaccharides And Bovine Colostrum Fractions, Maria X. Maldonado-Gomez Nov 2011

Studies On The Adherence Properties Of Plant Lectins And Bacterial Adhesins And Their Inhibition By Prebiotic Oligosaccharides And Bovine Colostrum Fractions, Maria X. Maldonado-Gomez

Dissertations, Theses, & Student Research in Food Science and Technology

To initiate colonization and infection, most microbial pathogens must first recognize and adhere to cells in the host tissues. Adherence inhibition, therefore, can be used as a strategy for preventing infectious disease. In this research, we developed a simplified model using plant lectins and HEp-2 tissue culture cells to simulate bacterial adhesins-receptors interactions. The effect of lectin ligands and two prebiotic carbohydrates, galactooligosaccharide (GOS) and polydextrose (PDX) was measured with this approach. Lectins that were able to bind to the target cells were inhibited in the presence of the cognate ligands and at least one of the prebiotic carbohydrates. The ...


The Bifidogenicity Of The Prebiotic Galactooligosaccharides, Lauren M. G. Davis Dec 2010

The Bifidogenicity Of The Prebiotic Galactooligosaccharides, Lauren M. G. Davis

Dissertations, Theses, & Student Research in Food Science and Technology

The goal of this research was to determine the effect of different doses of galactooligosaccharide (GOS) on the fecal microbiota of healthy adults, with a focus on bifidobacteria. The study was designed as a single-blinded study, with eighteen subjects consuming GOS-containing chocolate chews at four increasing dosage levels; 0, 2.5, 5.0, and 10.0 g. Subjects consumed each dose for 3 weeks, with a two-week baseline period preceding the study and a two-week washout period at the end. Cultural methods were used for bifidobacteria, Bacteroides, enterobacteria, enterococci, lactobacilli, and total anaerobes; culture-independent methods included denaturing gradient gel electrophoresis ...