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University of Nebraska - Lincoln

Dissertations, Theses, & Student Research in Food Science and Technology

Pathogens

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A Risk-Based Approach To Evaluate The Impact Of Interventions At Reducing The Risk Of Foodborne Illness Associated With Wheat-Based Products, Luis Sabillon Oct 2018

A Risk-Based Approach To Evaluate The Impact Of Interventions At Reducing The Risk Of Foodborne Illness Associated With Wheat-Based Products, Luis Sabillon

Dissertations, Theses, & Student Research in Food Science and Technology

Pathogens are emerging on previously unrecognized food vehicles, such as wheat flour, which is a widely consumed commodity. Despite its low water activity, wheat-based ingredients and products have been implicated in several foodborne outbreaks over the last few years, resulting not only in the recall of food products but also in consumer illnesses. As a raw agricultural commodity, wheat is exposed to naturally occurring microbiological contamination that may compromise the overall safety of flour and other derived products. To address the increased concern regarding the safety of wheat-based foods, the objectives of the present research study were designed to develop ...


Studies On The Adherence Properties Of Plant Lectins And Bacterial Adhesins And Their Inhibition By Prebiotic Oligosaccharides And Bovine Colostrum Fractions, Maria X. Maldonado-Gomez Nov 2011

Studies On The Adherence Properties Of Plant Lectins And Bacterial Adhesins And Their Inhibition By Prebiotic Oligosaccharides And Bovine Colostrum Fractions, Maria X. Maldonado-Gomez

Dissertations, Theses, & Student Research in Food Science and Technology

To initiate colonization and infection, most microbial pathogens must first recognize and adhere to cells in the host tissues. Adherence inhibition, therefore, can be used as a strategy for preventing infectious disease. In this research, we developed a simplified model using plant lectins and HEp-2 tissue culture cells to simulate bacterial adhesins-receptors interactions. The effect of lectin ligands and two prebiotic carbohydrates, galactooligosaccharide (GOS) and polydextrose (PDX) was measured with this approach. Lectins that were able to bind to the target cells were inhibited in the presence of the cognate ligands and at least one of the prebiotic carbohydrates. The ...