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Full-Text Articles in Life Sciences

Examination Of Egg White Proteins And Effects Of High Pressure On Select Physical And Functional Properties, Andrew Hoppe Dec 2010

Examination Of Egg White Proteins And Effects Of High Pressure On Select Physical And Functional Properties, Andrew Hoppe

Dissertations, Theses, & Student Research in Food Science and Technology

Egg white proteins have become an important and desirable ingredient to the food industry due to their functional properties which include gelling, foaming, and emulsification. Egg white is also well recognized as an excellent source of nutrition. The goal of this work was to determine the effects of high pressure (HP) treatment on egg white proteins. Specifically, experiments were conducted using Raman spectroscopy and pepsin digestibility to investigate structural changes. Pressure treatment at 400 to 800 MPa (5 minutes at 4°C) resulted in increased pepsin digestibility of egg white proteins ovalbumin, ovotransferrin, and lysozyme compared to heat-treated (85 to ...


The Bifidogenicity Of The Prebiotic Galactooligosaccharides, Lauren M. G. Davis Dec 2010

The Bifidogenicity Of The Prebiotic Galactooligosaccharides, Lauren M. G. Davis

Dissertations, Theses, & Student Research in Food Science and Technology

The goal of this research was to determine the effect of different doses of galactooligosaccharide (GOS) on the fecal microbiota of healthy adults, with a focus on bifidobacteria. The study was designed as a single-blinded study, with eighteen subjects consuming GOS-containing chocolate chews at four increasing dosage levels; 0, 2.5, 5.0, and 10.0 g. Subjects consumed each dose for 3 weeks, with a two-week baseline period preceding the study and a two-week washout period at the end. Cultural methods were used for bifidobacteria, Bacteroides, enterobacteria, enterococci, lactobacilli, and total anaerobes; culture-independent methods included denaturing gradient gel electrophoresis ...


Identification And Characterization Of Salmonella Serotypes Isolated From Pork And Poultry From Commercial Sources, Yulie Edith Meneses-GonzáLez Nov 2010

Identification And Characterization Of Salmonella Serotypes Isolated From Pork And Poultry From Commercial Sources, Yulie Edith Meneses-GonzáLez

Dissertations, Theses, & Student Research in Food Science and Technology

Salmonella is one of the leading causes of foodborne illness worldwide, and it is estimated that 1.4 million infections occur annually in the U.S. alone. The Premi®Test Salmonella (PTS), is a potential tool for rapid detection and identification of Salmonella serovars. The objective of this project was to evaluate the use of the PTS system as a serotyping tool to identify pork and poultry isolates obtained from vertically integrated operations and to characterize their antibiotic resistance. Two hundred isolates were evaluated. All isolates were serotyped using the traditional Kaufmann-White scheme and the PTS system. Among the isolates ...


Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For The Detection Of Pistachio Residues In Processed Foods, Pei Wen Lim Nov 2010

Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For The Detection Of Pistachio Residues In Processed Foods, Pei Wen Lim

Dissertations, Theses, & Student Research in Food Science and Technology

Pistachios (Pistacia vera) are popular snacks and consumption of pistachios is notably increasing due to their usage as ingredients for confections, ice cream, and baked goods. The increased consumption of pistachios may lead to a higher frequency of allergic sensitization and an increased prevalence of allergic reactions to pistachios. Trace amounts of undeclared pistachio allergens can pose serious health risks for food-allergic consumers, including severe anaphylactic reactions. A highly sensitive analytical method, sandwich-type enzyme-linked immunosorbent assay (ELISA) is applied as a critical tool for food manufacturers and food scientists to detect minute amounts of allergenic food residues in processed foods ...


The Effects Of High Pressure Processing On Peanut Sauce Inoculated With Salmonella, Tara K. Stiles Jul 2010

The Effects Of High Pressure Processing On Peanut Sauce Inoculated With Salmonella, Tara K. Stiles

Dissertations, Theses, & Student Research in Food Science and Technology

Recent Salmonella outbreaks have prompted the need for new processing options for peanut products. Traditional heating kill-steps have shown to be ineffective in lipid-rich matrices such as peanut products. High pressure processing is one such option for peanut sauce because it has a high water activity, which has proved to be a large contributing factor in microbial lethality due to high pressure processing. Four different formulations of peanut sauce were inoculated with a five strain Salmonella cocktail and high pressure processed. Results indicate that increasing pressure or increasing hold time increases log10 reductions. The Weibull model was fitted to ...


Development Of Fourier Transform Mid-Infrared Spectroscopy As A Metabolomic Technique For Characterizing The Protective Properties Of Grain Sorghum Against Oxidation, Emily Diane Sitorius May 2010

Development Of Fourier Transform Mid-Infrared Spectroscopy As A Metabolomic Technique For Characterizing The Protective Properties Of Grain Sorghum Against Oxidation, Emily Diane Sitorius

Dissertations, Theses, & Student Research in Food Science and Technology

DEVELOPMENT OF FOURIER TRANSFORM MID-INFRARED SPECTROSCOPY AS A METABOLOMIC TECHNIQUE FOR CHARACTERIZING THE PROTECTIVE PROPERTIES OF GRAIN SORGHUM AGAINST OXIDATION

Emily D. Sitorius, M.S. University of Nebraska, 2010 Advisor: Vicki L. Schlegel

Cellular oxidative damage has been linked to many chronic diseases including Alzheimer’s, diabetes, and cancer. Recent studies have shown that foods rich in antioxidants (i.e., grain sorghum) can protect against oxidative stress and thus reduce the risk of disease. However, a significant gap in knowledge exists in that dietary antioxidants are usually characterized by methods that determine free radical and reactive oxygen species’ scavenging capacity ...


Dynamic Predictive Model For The Growth Of Salmonella Spp. In Liquid Whole Egg And Risk Evaluation Of Egg White Hydrolysates Manufacturing Process For Spore Formers: Bacillus Cereus And Clostridium Perfringens., Aikansh Singh May 2010

Dynamic Predictive Model For The Growth Of Salmonella Spp. In Liquid Whole Egg And Risk Evaluation Of Egg White Hydrolysates Manufacturing Process For Spore Formers: Bacillus Cereus And Clostridium Perfringens., Aikansh Singh

Dissertations, Theses, & Student Research in Food Science and Technology

A dynamic model for the growth of Salmonella spp. in liquid whole egg under continuously varying temperature was developed and validated. A primary model (Baranyi model) was fitted to each temperature growth data and corresponding maximum growth rates were estimated. Pseudo-R2 values were greater than 0.97 for primary models. The maximum growth rates obtained from each primary model were then plotted against temperature and modeled using the modified Ratkowsky model. The pseudo-R2 and root mean square error were 0.99 and 0.06 h-1, respectively for the secondary model. A dynamic model for the prediction of ...