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University of Massachusetts Medical School

Cellular and Molecular Physiology

1999

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Full-Text Articles in Life Sciences

A Novel Membrane Protein Influencing Cell Shape And Multicellular Swarming Of Proteus Mirabilis, Nicole A. Hay, Donald J. Tipper, Daniel Gygi, Colin Hughes Apr 1999

A Novel Membrane Protein Influencing Cell Shape And Multicellular Swarming Of Proteus Mirabilis, Nicole A. Hay, Donald J. Tipper, Daniel Gygi, Colin Hughes

Microbiology and Physiological Systems Publications and Presentations

Swarming in Proteus mirabilis is characterized by the coordinated surface migration of multicellular rafts of highly elongated, hyperflagellated swarm cells. We describe a transposon mutant, MNS185, that was unable to swarm even though vegetative cells retained normal motility and the ability to differentiate into swarm cells. However, these elongated cells were irregularly curved and had variable diameters, suggesting that the migration defect results from the inability of these deformed swarm cells to align into multicellular rafts. The transposon was inserted at codon 196 of a 228-codon gene that lacks recognizable homologs. Multiple copies of the wild-type gene, called ccmA, for ...